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Perez, Moises Roberto Vallejo,Contreras, Hugo Ricardo Navarro,Herrera, Jesus A. Sosa,Avila, Jose Pablo Lara,Tobias, Hugo Magdaleno Ramirez,Martinez, Fernando Diaz-Barriga,Ramirez, Rogelio Flores,Vazqu The Korean Society of Plant Pathology 2018 Plant Pathology Journal Vol.34 No.5
Clavibacter michiganensis subsp. michiganesis (Cmm) is a quarantine-worthy pest in $M{\acute{e}}xico$. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy ($532nm\;{\lambda}$ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint. Our experiment was conducted with tomato plants in a completely randomized block experimental design (13 plants ${\times}$ 4 rows). The Cmm infection was confirmed by 16S rDNA and plants showed symptoms from 48 to 72 h after inoculation, the evolution of the incidence and severity on plant population varied over time and it kept an aggregated spatial pattern. The contagion process reached 79% just 24 days after the epidemic was induced. Micro-Raman spectroscopy proved its speed, efficiency and usefulness as a non-destructive method for the preliminary detection of Cmm. Carotenoid specific bands with wavelengths at 1146 and $1510cm^{-1}$ were the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively).
Perla Leon-Flores,Nayelli Najera,Elizabeth Perez,Blanca Pardo,Fiacro Jimenez,Dylan Diaz-Chiguer,Francisco Villarreal,Isabel Hidalgo,Guillermo Ceballos,Eduardo Meaney 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.7
Therapeutic approaches to decrease serum triglyceride (TG) concentrations are not successful mainly due to poor adherence or adverse effects of therapies. In consequence, the search for new low-cost and safer therapeutic alternatives is mandatory. Dark chocolate and cacao have shown promising results improving lipid profiles. Recently, using cacao by-products to reduce elevated cardiometabolic risk markers in an animal model of obesity induced by a high-fat diet and fructose, we showed that TGs, low-density lipoprotein cholesterol, and the TG/high-density lipoprotein (HDL) ratio decreased, suggesting that cacao by-products improved the metabolic function of obese animals. Based on these results, as a proof of concept, a blinded placebo-controlled study was implemented to explore the effects of cacao by-products on anthropometric and biochemical variables in a group of overweight subjects participating in a program composed of reduced-calorie-diet counseling plus a simple aerobic exercise plan. The results showed that counseling induced weight and abdominal circumference reductions in both groups. TGs did not change in the control group; however, TG decreased significantly by 54.9 mg/dL (27.9%) in the experimental group. The TG/HDL cholesterol ratio changed markedly (1.5) in the experimental group. The results reported suggest the use of cacao by-products as an alternative for the treatment of hypertriglyceridemia.
Eslava, Carlos,Sainz, Teresita,Perez, Julia,Fresan, Ma.Cristina,Flores, Veronica,Jimenez, Luis,Hernandez, Ulises,Herrera, Ismael The Microbiological Society of Korea 2002 The journal of microbiology Vol.40 No.2
Enteroaggregative E. coil (EAEC) is an important aethiological causal agent of diarrhea in people of developed and undeveloped countries. Different in vitro and in vivo models have been proposed to study the pathdgenic and immune mechanisms of EAEC infaction. The aim of this study was to analyze whether BALB/c mice could be used as an animal model to study EAEC pathogenesis Six-week-old BALB/c mice were inoculated with EAEC strain 042 (044:H88) nalidixic acid resistant, and re-inoc-ulated ten days after. Mice feces were monitored for the presence of the EAEC strain over a period of 20 days . Bacteria were enumerated on MacConkey agar containing 100$\mu$g of nalidixic acid per ml. Results showed that 35% of the animals were colonized for 3 days, 15% for 5 and 10% for more than 7 days . After re-inoculation only 16% of the animals remained colonized for more than 3 days. During the necropsy, the intestinal fluid of same of the infected animals presented mucus and blood. Six of these fluids showed the presence of IgA antibodies againset Pet toxin and IgG natibodies raised against the toxin were also detected in the animal serum. Histopathologic evidence confirms the stimulation of mucus hypersecretion, an increased amount of goblet cells and the presence of bacterial aggregates in the apical surfaces of intestinal epithelial cells. Edema was present in the submucosa. These results suggest that BALB/c mice could be used as an animal model for in vivo study of EAEC infection.
Single-step genomic evaluation for growth traits in a Mexican Braunvieh cattle population
Jonathan Emanuel Valerio-Hernandez,Agustin Ruiz-Flores,Mohammad Ali Nilforooshan,Paulino Perez-Rodriguez Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.7
Objective: The objective was to compare (pedigree-based) best linear unbiased prediction (BLUP), genomic BLUP (GBLUP), and single-step GBLUP (ssGBLUP) methods for genomic evaluation of growth traits in a Mexican Braunvieh cattle population. Methods: Birth (BW), weaning (WW), and yearling weight (YW) data of a Mexican Braunvieh cattle population were analyzed with BLUP, GBLUP, and ssGBLUP methods. These methods are differentiated by the additive genetic relationship matrix included in the model and the animals under evaluation. The predictive ability of the model was evaluated using random partitions of the data in training and testing sets, consistently predicting about 20% of genotyped animals on all occasions. For each partition, the Pearson correlation coefficient between adjusted phenotypes for fixed effects and non-genetic random effects and the estimated breeding values (EBV) were computed. Results: The random contemporary group (CG) effect explained about 50%, 45%, and 35% of the phenotypic variance in BW, WW, and YW, respectively. For the three methods, the CG effect explained the highest proportion of the phenotypic variances (except for YW-GBLUP). The heritability estimate obtained with GBLUP was the lowest for BW, while the highest heritability was obtained with BLUP. For WW, the highest heritability estimate was obtained with BLUP, the estimates obtained with GBLUP and ssGBLUP were similar. For YW, the heritability estimates obtained with GBLUP and BLUP were similar, and the lowest heritability was obtained with ssGBLUP. Pearson correlation coefficients between adjusted phenotypes for non-genetic effects and EBVs were the highest for BLUP, followed by ssBLUP and GBLUP. Conclusion: The successful implementation of genetic evaluations that include genotyped and non-genotyped animals in our study indicate a promising method for use in genetic improvement programs of Braunvieh cattle. Our findings showed that simultaneous evaluation of genotyped and non-genotyped animals improved prediction accuracy for growth traits even with a limited number of genotyped animals.
Macarena GROSS ARIZA,Reyes ABAD FLORES,Maria F. Carrascal Perez CARRA 서울대학교 환경대학원 2020 環境論叢 Vol.66 No.-
Spread throughout the city, the “corralas” are essential pieces of Sevilla’s urban fabric (Spain). These spaces that were particularly shaped for an affordable co-life and craftwork activities have become a referent for contemporary creative communities, which have not just appropriated them but have emulated them. In obsolete industrial spaces, these social groups recreate this traditional way of life around a courtyard: the key element of vernacular residential architecture in this region. Corrala’s project of colife has allowed current creative workers to live and develop their practices, while, in a reciprocal process, they have become protectors of its meaning and mixed forms, an ethnological heritage in danger. This research focuses on the specific case of “El Pelícano”, a former cork factory inaugurated in the decade of the 1920’s, and its suitability for transformation into a contemporary creative venue assuming the corrala concept. The analysis is based on crossed methodologies: direct observation, interviews to the creators, participation in collective meals and other activities. A photo-shoot of El Pelícano by artist Macarena Gross presents its atmosphere and its phenomenological values in an attempt to reclaim the place of the creative corrala in the memory of this city.
Female Breast Cancer Incidence and Mortality in Mexico, 2000-2010
Anaya-Ruiz, Maricruz,Vallejo-Ruiz, Veronica,Flores-Mendoza, Lilian,Perez-Santos, Martin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3
The objective of this study was to investigate the recent incidence and mortality trends for breast cancer in Mexican females. Data between 2000 and 2010 from the Department of Epidemiology of the Ministry of Health, and International Agency for Research on Cancer (IARC) were analyzed. Age-standardized rates (ASRs) and annual percent changes (APCs) were calculated. The absolute incidence and mortality rates of breast cancer increased: 3,726 and 4,615 in 2000 to 8,545 and 4,966 in 2010, respectively. Incidence increased over time in all age groups tested, the 60-64 age group had the highest ASR (57.4 per 100,000 women in 2010), while the 20-44 age group had the lowest ASR (12.3 in 2010). The results show that incidence of breast cancer has increased in Mexico during last one decade, especially among older women, while the downturn observed in mortality mainly reflects improved survival as a result of earlier diagnosis and better cancer treatment.
Essential oil impregnation into graphene sponges with electric desorption control
Mendez, Jose Antonio Cabello,Bueno, Jose de Jesus Perez,Valencia, Jorge Ivan Mendoza,Soto, Jonathan Soto,Lopez, Maria Luisa Mendoza,Guerrero, Mizraim Uriel Flores Techno-Press 2022 Advances in nano research Vol.12 No.6
This work shows the impregnation of scents using a graphene sponge (GS). This was functionalized by the modified Hummers method, pursuing to add different functional groups. It is proposed to achieve the release and seek to control it through electrical potential applied to the graphene sponge with essential oils. The graphene sponge was functionalized and steeped with two kinds of oil. The electrochemical study demonstrates the variation in the electrochemical behaviour of the functionalized graphene sponge without and impregnated with oil. The release of the oil and its aromatic scents was carried out by applying an electrical potential of 30 V, with a release rate of 1.86 mg/min. The heating of the sample that causes the release of oil, associated with the electrical resistance of the system, reaches temperatures of about 150℃. The essential oils, graphene sponge, surfactant, graphene sponge with essential oils, graphene sponge recuperated after applying electric potential, graphene sponge recovered by temperature and dipropylene glycol (DPG) were characterized using Fourier transformed infrared spectroscopy (FTIR), digital microscopy, and x-ray photoelectron spectroscopy (XPS).
N.A. Medellin-Castillo,R. Leyva-Ramos,E. Padilla-Ortega,R. Ocampo Perez,J.V. Flores-Cano,M.S. Berber-Mendoza 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
The adsorption of fluoride from water on bone char (BC) was investigated in this work, and the fluorideadsorption capacity of BC was compared to that of hydroxyapatite (HAP). The adsorption capacity of BCand HAP drastically increased while decreasing the pH from 7.0 to 5.0. Furthermore, the fluorideadsorption on BC was due to its HAP content and was not considerably affected by the presence of theanions Cl-, HCO3-, CO32-, SO42-, NO3- and NO2-. The mechanism of fluoride adsorption on BC wasattributed to electrostatic interactions between surface charge of BC and fluoride ions in solution.
Infertility in Pediatric Cancer Survivors: A Necessary Evil or a Potentially Avoidable Sequel?
Torres-Roman, Smith Junior,Conislla-Espinoza, Ismael,Gutierrez-Flores, Katherin Estefany,Bazalar-Palacios, Janina,Paredes-Perez, Napoleon Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.10