수면 부유 방법에 의한 연초 육묘

洪淳達,石泳善,朴孝澤 충북대학교 엽연초연구소 1999 煙草硏究 Vol.17 No.-

Carbonized rice hull, perlite, and vermiculite were mixed and investigated for the probability of tobacco transplant production by means of plug and transfer float system. These seedling bed materials in condition of float system have gaseous phase ranged from 6% to 13% showing a proper growth of tobacco seedling. However compost media mixed with soil, sand, and compost in condition of float system led to the shortage of respiration in root system by very low proportion of gaseous phase skewing a wicked growth of seedling. Absorption of heat by floating water for the daytime increased rhizosphere temperature in condition of float system during the night and low temperature season resulting in better growth of tobacco seedling. Consequently transplant production of tobacco seedling by float system with mixed materials of carbonized rice hull, perlite, and vermiculite was thought to be desirable method for manufacturing production system of transplant considering the effective management of water, nutrients, and temperature of seedling bed materials.

[Gln]1.4,[Phe]11 - Neurotensin 의 합성

박두천,홍남주,박영택 國立 昌原大學校 産業技術硏究所 1989 産技硏論文集 Vol.3 No.-

인체 대장암 및 자궁경부암에서 PCR-SSCP법을 이용한 Ki-ras 암유전자의 점돌연변이에 관한 연구

박영홍,백낙환,김현찬,김상효,홍관희,김기태,이기영 인제대학교 1994 仁濟醫學 Vol.15 No.2

인체 대장암 20례와 자궁경부암 10례의 암조직을 대상으로 c-Ki-ras 유전자 codon 12와 13에서의 점돌연변이를 알아보고자 polymerase chain reaction(PCR)과 single-strand conformation polymorphism(SSCP) 검사법을 시행하였다. 대장암의 경우 20례중 9례(45%)에서, 자궁경부암의 경우 10례중 1례(10%)에서 양성으로 나왔다. 대장암에서는 codon 12에서 GGT가 TGT로의 치환이 4례로서 가장 많았으며, AGT, CGT로의 치환이 각 1례였고 codon 13에서는 GGC가 GAC로의 치환이 2례, TGC로의 치환이 1례인 것으로 나타났다. 자궁경부암의 경우 codon 12에서 GGT가 AGT로의 치환이 1례인 것으로 나타났다. In an attempt to clarify the role of genetic alteration in the genesis of human colorectal and cervical cancers, tissue specimens from 20 patients with colorectal cancer and 10 patients with cervical cancer were examined for the presence of point mutation in K-ras2 exon 1 by single strand conformation polymorphism analysis of PCR product. Exon I of c-Ki-ras2 was amplified by polymerase chain reaction(PCR) and comparison was made between the normal and mutated genes by nondenaturing polyacrylamide gel electrophoresis(PAGE) of PCR product and nucleotide sequence analysis using asymmetric PCR with direct sequencing. Genomic DNA from white blood cells were used as normal control and those from A427 cell line were used as mutated control. 1.PCR product from A427 cell line showed a distinct migration shift pattern compared to the normal control in PAGE and the direct sequencing indicated that nucleotide sequence of codon 12 was mutated from GGT to GAT. In addition, there was a loss of normal allele in A427 cell line. 2.PCR product from 9 cases(45%) out of a 20 colon cancer patients showed migration shifts in PAGE, and all of these 9 patients invariably demonstrated mutation of ras gene, either in codon 12(6 cases:30%) or 13(3 cases:15%). The base substitutions in codon 12 were: from GGT to AGT(1), CGT(1), or TGT(4). Base changes in codon 13 were from GGC to TGC(1) or GAC(2). 3.PCR product from 1 cases(10%) out of a 10 cervical cancer patients showed migration shrifts in PAGE, Invariably demonstrated mutation of ras gene in codon 12. The base substitutions was from GGT to AGT(1) TQE incidence of point mutation of c-Ki-ras in colon cancer was high, however, it was low in cervical cancer, which showed the relation of ruts gene mutation with colon cancer. PCR -SSCP analysis is a simple, rapid and efficient method of detection of point mutation, especially when dealing with multiple samples.

소아와 성인의 HBV 관련 신병증에서 HBV pre-S/S DNA 돌연변이의 분석

박영홍,박기호,정우영 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Purpose: The aim of this study was to investigate the mutations of HBV pre-S/S gene in HBV-associated nephropathy patients and to compare the patterns of mutations between child and adult patient, which may suggest the relationship of HBV with these diseases. Method: Seventeen fresh frozen or paraffin-embedded renal tissue samples were obtained from child or adult patients with HBsAg-positive and nephropathy. HBV pre-S/S regions were amplified with PCR and subjected to direct sequencing. Mutations were analyzed at pre-S1, pre-S2 and S gene regions and the difference of pattern was evaluated between adult and child. Results: Seventeen PCR products were direct-sequenced, of which twelve(70.6%) had the mutations at pre-S1 region. Point mutations were detected from all of 12 cases(100.0%) and resulted in amino acid substitutions as Q8OP(CAG->CCG) and L112I(CTA→ATA). Deletion mutations were detected in 2 patients(16.7%) of which one had 27 bp(3149-3175) deletion and the other 2 deletions including l5 bp(2847-2861) and 60 bp(3143-3202) deletions. The mutations at pre-S2 region were detected in 11 cases(64.7%) of which 10(90.9%) had point mutations. Amino acid substitution of T37I due to the point mutation from ACT to ATT was found in 4 cases. Deletion mutations were detected in 3 cases(27.3%) of which one had 66 bp(3203-3213,1-55) deletion which was continuous with the pre-S1 deletion. The other two had 48 bp(nt8-55) and 60 bp(nt3208-3213, 1-54) deletions, respectively. The mutations at S gene were found from all of 17 cases and are exclusively the point mutations but not the deletion mutation. At 'a' determinant(aa124-147), three amino acid substitutions including Ⅰ126N, T131A and G145R were detected. Most of mutations with high frequency and/or amino acid substitution were detected from children, including at Q8OP at pre-S1, T37l at pre-S2 and T131A at S gene. Conclusion: We could find the various mutations including point mutations and deletion mutations at HBV pre-S/S gene in kidney tissue from patients with HBV-associated nephropathy. The comparison of the mutation between the child and adult patients showed the significant difference of mutation pattern.

Restriction fragment length polymorphism에서 Liquid handling system과 Denaturing High performance liquid chromatography를 이용한 돌연변이의 검출

박영홍 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

There had been a great need for the more efficient and rapid method to detect the mutations in DNA. The advance of molecular biology and biotechnology enable the processing and analysis automated including liquid handling system and denature HPLC. The author tried to investigate the efficiency and efficacy of liquid handling system and denaturing HPLC for the detection of the mutations from mitochondrial DNA. Using liquid handling system, author purified the genomic and the PCR-amplified mitochondrial DNA, which were subjected to the treatment of restriction enzyme HaeⅢ, which were analysed by denature HPLC. Restriction fragment length polymorphism showed the four band including 174bp, 242bp, 285bp and 545bp at both cell lines. However 50:50 mixture revealed single-base mutation through the two peaks at 285bp band. These results demonstrated that the combination of both machines was very rapid and efficient for the detection of mutations and introduced the more convenient and reliable data. Especially, large-scale processing using 96 well can make the combination of liquid handling system and denaturing HPLC more valuable.

鐵棒 1回, 2回 및 3回 뒤공중돌아내리기의 比較 分析

申榮吉,金柄斗,安禹洪,金正子,吳東燮,朴宇奎 慶北大學校 體育科學硏究所 1987 체육과학연구지 Vol.3 No.-

The purpose of this study was to offer gymnasts data for successfully performing triple back somersault dismount(TBSD) and for designing training programs to progress gymnasts to the advanced dismount skills from the horizontal bar through comparative analysis of selected biomechanical factors of single back somersault dismount(SBSD), double back somersault dismount(DBSD) and TBSD. The subjects employed were a total of 4 male middle and high school gymnasts aged between 15 and 16 years. A motion-picture camera, fliming at a nominal rate of 54 frames/s, was used to record the performance of subjects on each trial. This camera was placed at a distance of 15 m from the inward pole of horizontal bar with its optical axis at right angle to this pole. The results obtained are summarized as follows: The mean release angle was 80.5° for the SBSD, 77.3° for the DBSD and 71.3° for TBSD. There were significant differences between the SBSD and the TBSD(p<0.01), the DBSD and the TBSD(p<0.05) but no significant difference between the SBSD and the DBSD. The angular velocity at release was 4.533 ㎭/s for the SBSD, 5.338 ㎭/s for the DBSD and 6.020 ㎭/s for the TBSD. There was a significant difference among these velocities(p<0.05, p<0.01). The linear velocity at release was 5.458m/s for the SBSD, 5.750m/s for the DBSD and 6.083m/s for the TBSD. In this case, we had a significant difference only between the SBSD and the other two somersaults(p<0.05, p<0.01). The shoulder angle at release was 170.5° for the SBSD, 167.0° for the DBSD and 154.3° for the TBSD. There were significant differences between the SBSD and the TBSD(p<0.05), the DBSD and the TBSD(p<0.05) but no significant difference between the DBSD and the TBSD. The hip and knee angle at release were increased respectively by increasing the number of somersault but showed no significant difference among these angles. The mean flight time from release to landing for each somersault was calculated; it took the subjects 1.315s to perform the SBSD, 1.345s to perform the DBSD and 1.416s to perform the TBSD. There was a significant difference among these somersaults with respect to time(p<0.05, p<0.01). The mean maximum height was 3,633m for the SBSD, 3,720m for the DBSD and 3,780m for the TBSD. There was a significant difference only between the SBSD and the other two somersaults. The mean horizontal distance was decreased respectively by increasing the number of somersault but showed no significant difference among these somersaults with respect to distance. The smallest hip angle during flight in the SBSD was 115.3° at 0.317s after the subject left the bar, for the DBSD it was 48.2° at 0.621s while that of the TBSD was 32.3° at 1,102s after release. The relationship between the time the smallest hip angle observed and the total flight time was determined; it took 24.1% of the total flight time of the SBSD to reach the smallest hip angle. In the DBSD, it took 46.2% of the total flight time to reach for the smallest hip angle and in the TBSD 77.8% of the total flight time to reach the smallest hip angle. From the above results, the gymnast would need to (1) increase angular velocity before release by manipulating of body shape, (2) change the release angle and (3) stay tuck position longer by shortening as quickly as possible the angle of each body joint to perform successfully the TBSD. Also to be able to perform quadruple back somersault dismount, a gymnast would need flight time of a minimum 1,782s.

한국형 후각검사 KVSS Test의 개발

홍석찬,유영석,김은서,김석천,박수홍,김진국,강성호 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

한국인의 간과 콩팥조직 내 수은 함유량의 참고치

최병선,박영주,권일훈,홍연표,박정덕 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.2

This study was conducted to investigate the reference value of mercury (Hg) in liver and kidney of Korean population. The mercury concentration in 244 'sudden and unexpected death' autopsies (male: 180, female: 64) aged from 0 to87 years was analyzed. The concentration of mercury was measured by atomic absorption spec(Perkin -Elmer Model 5100) with mercury/hydride generating system (FIAS 400). The contents of mercury in liver and kidney fitted well the log-normal distribution rather than normal distribution. Geometric mean concentration of mercury in liver and kidney was 0.115 ㎍/g wet weight and 0.149 μg/g wet weight, respectively. Geometric mean concentration of mercury in female was higher than in male (p<0.01). The mercury content in liver and kidney increased with age up to the forties and slightly decreased there-after, The regression model of mercury deposit in liver and kidney by age was predicted as the following equation : Log KHg=-1.0576+0.0045·Age -0.0001·Age²+0.0873·Sex, Log KHg=-1.0576+0.0152·Age-0.0002·Age²+0.1935·Sex. The liver burden of mercury was estimated to be 158.3~161.3μg in male and 163.0-166.9 ㎍ in female. The kidney burden of mercury was estimated to be 42.0~42.9 ㎍ in male and 55.5 ∼57.1 μg in female.

구순구개열 환아에서의 치조골이식

조해성,박재홍,김광철,최성철,이긍호,최영철 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

치조파열 및 구개열과 같은 선천성 기형은 이환된 환자에게 기능적, 심미적으로 많은 문제점을 야기하므로 정상으로 회복시켜 주는 것은 중요한 문제이다. 장기간 방치될 경우 영양장애, 구강위생 불량, 호흡기 간염, 언어 장애, 악안면 변형, 그리고 정신적인 문제 등이 복합적으로 발생할 수 있다. 따라서 구순구개열 환자의 치료는 여러 전문 치료 분야의 복합적인 접근이 필요하다. 골이식은 구순구개열 환자에 있어 중요한 치과치료 단계이다. 치아의 맹출과 치열의 안정화를 위해 치열궁은 골격손이 없이 완전해져야 한다. 또한 치열궁의 파열이 있는 부위로는 정상적인 교정적 치아이동이 곤란하다. 따라서 구순구개열 환자에 있어 골이식은 광범위하게 적용되고 있는 외과적 술식이다. 치조골을 이식함으로써 치조열은 안정화되고, 견치 또는 절치가 이식부위로 이동할 수 있게 된다. 그리고 골 이식 후, 교정을 통해 치조열 부위의 공간을 폐쇄함으로써 보철 치료 없이 치열을 재형성할 수 있다. 골이식술에는 다양한 이식재료가 사용되었다. 자가골을 이식할 경우 장골이 가장 선호되며, 그 밖에 경골, 늑골, 두개골, 하악골을 이용하기도 한다. 그리고 골이식은 골이식 시기에 따라 일차골이식, 조기 이차골이식, 이차골이식, 만기 이차골이식으로 구분할 수 있다. 이차골이식은 혼합치열기 말경에 시행되는 것을 말하며 가장 좋은 것으로 받아들여지고 있다. 영구 견치가 맹출되기 전에 이차골이식을 시행하면 인접치아의 맹출과 보존에 도움이 된다. 본 증례에서는 치조구개파열 환자의 장골에서 골을 채취하여 골 이식을 시행한 후, 골이 채워진 이식 부위에 인접 치아가 성공적으로 이동하거나 치축이 개선되고 교정치료를 통해 치열궁 배열의 만족스러운 결과를 얻었기에 이를 보고하는 바이다 Cleft lip and palate are congenital craniofacial malformation. Reconstruction of dental arch in patient with alveolo-palatal clefts is very important, because they have many problems in functions and esthetics. Malnutrition, poor oral hygiene, respiratory infections, speech malfunctions, maxillofacial deformity, and psychological problems may be occured without proper treatment during the long period of management of the cleft lip and palate. So the treatment should be managed with a multidisciplinary approach. Bone grafting is a consequential step in the dental rehabilitation of the cleft lip and palate patient. A complete alveolar arch should be achieved of the teeth to erupt in and to form a stable dentition. And the presence of the cleft complicate the orthodontic treatment. Therefore bone grafting in patients with cleft lip and palate is a widely adopted surgical procedure. Grafted bone stabilizes the alveolar process and allows the canine or incisor to move into the graft site. After the bone grafting, orthodontic closure of the maxillary arch has become a common practice for achieving dental reconstruction without any prosthodontic treatment. Various grafting materials have been used in alveolar clefts. Iliac bone is most widely fovoured, but tibia, rib, cranial bone, mandible have also been used. And according to its time of occurrence, the bone graft may be divided into primary, early secondary, secondary, late secondary. Bone grafting is called secondary when performed later, at the end of the mixed dentition. It is the most accepted procedure and has become part of treatment of protocol. A secondary bone graft is performed preferably before the eruption of the permanent canine in order to provide adequate periodontal support for the eruption and preservation of the teeth adjacent to the cleft. In this report, we report here on a patient with unilateral cleft lip and palate, who underwent iliac bone graft. The cleft was fully obliterated by grafted bone in the region of the alveolar process. The presence of bone permitted physiologic tooth movement and the orthodontic movement of adjacent tooth into the former cleft area. Satisfactory arch alignment could be achieved in by subsequent orthodontic treatment.

시냅토좀(synaptosome), 성장원추(growth cone), 성장원추막(growth cone membrane)의 분리

이상경,박영홍 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Under particular conditions of nervous tissue homogenization, the presynaptic terminals of neurons separate from the axons, forming structures called synaptosomes, which contain mainly mitochondria. cytoplasm, and synaptic vesicles. They are surrounded by intact presynaptic membrane, which sometimes may be attached to post-synaptic membrane fragments. These synaptosomes maintain the morphological characteristics, the chemical composition, and the metabolism of the intact synapses, thus allowing isolation of synaptic plasma membranes. Growth cone is a specialized region at the tip of a growing neurite that is responsible for guiding neuronal projections to their proper targets, a goal achieved through its capacity to interpret and integrate a large number of extracellular and intracellular signals. The purpose of this review article is to summarize the methods of collecting synaptosome, growth cone granule, and growth cone membrane.