Dynamics of a Mobile RNA of Potato Involved in a Long-distance Signaling Pathway

Hye Lin Park,Sang-gon Suh 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Cost-Effectiveness of Anti-Viral Treatment in Patients with Immune-Tolerant Phase Chronic Hepatitis B

( Hye-lin Kim ),( Gi-ae Kim ),( Jae-a Park ),( Hye-rim Kang ),( Eui- Kyung Lee ),( Young-suk Lim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Currently, antiviral therapy for chronic hepatitis B (CHB) patients in immune tolerant (IT) phase is generally not recommended. There has been a need for studies assessing benefits of antiviral therapy in IT-phase. A recent study showed that untreated IT-phase patients had higher risk of hepatocellular carcinoma (HCC) than treated immune active (IA) phase patients. We aimed to evaluate the cost-effectiveness of starting antiviral treatment from IT-phase (IT-Tx) compared to delaying the treatment to IA-phase (IA-Tx). Methods: We designed a Markov model to compare expected costs and quality-adjusted life-years (QALYs) between IT-Tx group and IA-Tx group from healthcare system and societal perspectives. Transition probabilities and costs were obtained from a cohort of 4,965 HBeAg-positive, treatment-naive CHB patients at Asan Medical Center. Literature review was conducted for other parameters. Cost and effectiveness were discounted at a 5% annual rate, and incremental cost-effectiveness ratio (ICER) was calculated for 10-year horizon and evaluated with various HCC risks. Results: The cost-effectiveness analysis showed that IT-Tx group had ₩6,996,562 incremental costs and additional 0.294 QALY per patient compared to IA-Tx group with 10-year cumulative HCC risk of 10% (base-case). ICER was ₩23,819,529/QALY, which was borderline high of the cost-effectiveness threshold (₩20,000,000/QALY) in Korea. As HCC risk increased, IT-Tx became acceptable in cost-effectiveness. When the HCC risk increased over 11.8%, ICER went below the threshold. The analysis including the cost of lost productivity showed that IT-Tx was dominant with HCC risk greater than 4.6% (ICER<0). Conclusions: To start antiviral therapy for CHB patients in IT-phase was borderline high cost-effective from healthcare system perspective dealing with the only medical costs, however, it was a dominant strategy in view of societal perspective covering also the costs for lost productivity.

Association between Dietary Inflammatory Index and the Risk for Protein Energy Wasting in Male Hemodialysis Patients

Hye Lin Ahn,Yun Jung Yang,Se Lin Kim,Sumin Heo,Sol Been Park,Yeo Gyeong Han,Hyunjin Ryu,Curie Ahn,Kook-Hwan Oh,Soo Jin Yang 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Molecular Characterization of Phytochrome-interacting Nascent Polypeptide Associated Complex Protein in Arabidopsis thaliana

Park, Hye-Lin,Cho, Man-Ho,Shin, Dong-Ho,Bhoo, Seong-Hee,Hahn, Tae-Ryong The Korean Society for Applied Biological Chemisty 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.2

Nascent polypeptide associated complex (NAC) proteins bind to newly synthesized polypeptide chains from ribosome and are involved in various physiological processes. An in vitro pull-down assay demonstrated that the previously identified NAC protein interacts with phytochromes. Phytochrome-interacting NAC protein (PNAC) was expressed in the cytosol, and translocation of the PNAC protein into nucleus by irradiation was not observed. These findings suggest that PNAC is a possible cytosolic partner protein to phytochromes. Histochemical analysis of PNAC promoter-${\beta}$-glucuronidase (GUS) transgenic plants showed that PNAC expression was increased by light treatment, especially red light, indicating that phytochromes likely modulate PNAC gene expression.

Cost-Effectiveness of Hepatocellular Carcinoma Surveillance with Magnetic Resonance Imaging (MRI) in Cirrhosis Patients Using a Prospective Cohort Data

( Hye-lin Kim ),( Jihyun An ),( Seung-hoo Park ),( Jae-a Park ),( Young-suk Lim ),( Eui-kyung Lee ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hepatocellular carcinoma (HCC) surveillance using ultrasonography (US) is recommended for cirrhotic patients to detect HCC in earlier stages. But in high-risk patients with cirrhosis, surveillance by magnetic resonance imaging (MRI) with liver-specific contrast results in earlier detection of HCC than US. The study aimed to estimate the cost-effectiveness of semiannual surveillance with MRI compared to US in cirrhotic patients at high risk for HCC. Methods: We designed a Markov model to compare expected costs and quality adjusted life years (QALY) between MRI and US over 20 years of time horizon from the limited societal perspective. The starting age of the cohort was 50 years old, and the cycle length was 6 months. Transition probabilities and costs were obtained mainly from a prospective cohort - the PRIUS study (NCT01446666). Literature review was conducted for HCC detection rate of US and utility weights. Effectiveness was measured life year (LY) gained and QALY. Cost and effectiveness were discounted at a 5% annual rate. An incremental cost-effectiveness ratio (ICER) was calculated and tested with deterministic and probabilistic sensitivity analyses. Results: The cost-effectiveness (CE) analysis indicated that MRI had ₩3,219,387 incremental costs, 0.521 incremental LY, and 0.313 incremental QALY per patient as compared to US. ICER were ₩6,175,874/LY and ₩10,621,005/QALY. Sensitivity analysis demonstrated that the annual HCC incidence was the most sensitive, ICER was estimated below the CE threshold (₩20,000,000/QALY) when the annual HCC incidence was over 4%. In sensitivity analysis, the annual HCC incidence and the cost ratio of MRI/US were varied, which showed that as HCC incidence increased, higher MRI/US cost ratio became acceptable in cost-effectiveness. Conclusions: This study demonstrated that semiannual MRI surveillance for HCC in cirrhotic patients at high-risk cost-effective compared to US. This results is helpful in determining the surveillance method considering the extent of patient's risk for HCC.

Molecular Characterization of Phytochromeinteracting Nascent Polypeptide Associated Complex Protein in Arabidopsis thaliana

Hye Lin Park,조만호,신동호,부성희,Tae-Ryong Hahn 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.2

Nascent polypeptide associated complex (NAC) proteins bind to newly synthesized polypeptide chains from ribosome and are involved in various physiological processes. An in vitro pulldown assay demonstrated that the previously identified NAC protein interacts with phytochromes. Phytochrome-interacting NAC protein (PNAC) was expressed in the cytosol, and translocation of the PNAC protein into nucleus by irradiation was not observed. These findings suggest that PNAC is a possible cytosolic partner protein to phytochromes. Histochemical analysis of PNAC promoter-β-glucuronidase (GUS) transgenic plants showed that PNAC expression was increased by light treatment,especially red light, indicating that phytochromes likely modulate PNAC gene expression.

Cancer Prevalence among Physicians in Korea: A Single Center Study

Hye Lin Kim,Hae Jin Park,Yun Hye Sim,Eun Young Choi,Kyung Won Shim,Sang Wha Lee,Hong Soo Lee,전혜진 대한가정의학회 2016 Korean Journal of Family Medicine Vol.37 No.2

Background: There is little research regarding whether working as a physician affects cancer risk. Moreover, thereis no research on cancer prevalence among physicians in Korea. This study utilized the Korea National Cancer IncidenceDatabase to determine whether the prevalence of cancer among physicians differs from the prevalence ofcancer within the general population. Methods: We analyzed the medical records of a representative sample of 382 doctors who underwent a health examinationbetween 2010 and 2013 at a health examination center in a Ewha Womans University Medical Center. Cancer incidence was measured as cases that were eventually diagnosed as cancer according to a biopsy. Results: We collected medical records from 382 physicians (mean age, 51.9±8.1 years) and calculated the standardizedprevalence ratios compared to the general population. Thirty physicians (9 male and 21 female) were identifiedas having cancer. Physicians had a significantly higher prevalence of cancer compared to the general population. Cancer prevalence in male physicians was found to be 2.47 times higher than the prevalence expected withinthe general population (P=0.006). Among female physicians, cancer prevalence was 3.94 times higher than that inthe general population (P<0.001). Conclusion: This study revealed that physicians had a higher prevalence of cancer compared to the general populationin Korea, which suggests that there may be a problem present in the health care of physicians. Changes to theworking environment of physicians will be needed to reduce the high prevalence of cancer among physicians.

Biochemical characterization of a regiospecific flavonoid 3'-O-methyltransferase from orange

Park Hye Lin,Bhoo Seong Hee,Lee Sang-Won,Cho Man-Ho 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Citrus plants have diverse methoxyflavonoids including, chrysoeriol, isosakuranetin, and nobiletin. In plants, O-methyltransferases (OMTs) participate in the methylation of a vast array of secondary metabolites, including flavonoids, phenylpropanoids, and alkaloids. To identify functional OMTs involved in the formation of methoxyflavonoids, orange (Citrus sinensis) OMT (CsOMT) genes were retrieved from the Citrus Genome Database. The phylogenetic relationships with functional OMTs suggested that three CsOMTs, CsOMT15, CsOMT16, and CsOMT30, are possible candidates for flavonoid OMTs (FOMTs). These CsOMTs were heterologously expressed in Escherichia coli, and their OMT activity was examined with flavonoid substrates. Of the examined CsOMTs, CsOMT16 catalyzed the regiospecific 3’-O-methylation of flavonoids to the respective 3’-methoxyflavonoids. A kinetic study demonstrated that CsOMT16 accepts diverse flavonoids as a substrate with a comparable preference. The flavonoids eriodictyol, luteolin, and quercetin were efficiently converted to homoeriodictyol, chrysoeriol, and isorhamnetin by CsOMT16- transformed E. coli cells, respectively. These findings suggest that CsOMT16 contributes to the methoxyflavonoid formation in orange and is applicable to the biotechnological production of 3’-methoxyflavonoids.

Biogas Upgrading with Trickling Pall ring Filter

Hye Lin Park,Hang Bae Jun 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

구리전극이 설치된 미생물 전기화학적 혐기성 소화조에서 메탄올 생성

박혜린 ( Hye-lin Park ),전항배 ( Hang-bae Jun ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2019 No.-

혐기성 소화는 런던 협약이후 유기성 폐기물의 분해와 온실가스 배출량 감축을 동시에 대응할 수 있는 방법중 하나로 주목받고 있다. 하지만 혐기성 미생물의 느린 성장 속도와 긴 수리학적 체류시간이 요구되며 환경조건에 민감하여 안정적인 운전이 쉽지 않다. 이를 극복하기 위한 방안으로 생물전기 화학적 혐기성 소화조(BEAD-SBR_Cu)를 이용하여 전압을 걸어줌으로써 혐기성 소화 미생물의 활성을 높이고 안정화에 단계에 빨리 도달하도록 하는 연구가 활발히 진행되고 있다. 또한, Cu가 코팅된 환원 전극에서는 CO₂와 H₂가 메탄올로 환원되기 쉽다고 알려져 있다. 따라서 본 연구에서는 구리가 코팅된 환원 전극을 사용한 BEAD-SBR_Cu에서 메탄올의 대사와 미생물 군집의 변화를 조사 하였다. BEAD-SBR_Cu에서는 구리가 코팅된 환원 전극에 전압을 걸어 음식물폐기물에서 메탄올을 생성하였다. BEAD-SBR_Cu는 혐기성 소화조(AD-SBR)보다 메탄올 제거 및 메탄 생산 효율이 더 높았다. BEAD-SBR_Cu의 메탄 생성과 pH는 높은 유기 부하율 (OLR) 하에서도 안정적이었다. 높은 OLR에서 BEAD-SBR_Cu는 hydrogenotrophic methanogens는 32.2%에서 60.0%로 증가하였고, hydrogen-dependent methylotrophic methanogens는 19.5%에서 37.7%로 증가하였다. 메탄올을 BEAD-SBR_Cu에 단일 기질로 직접 주입한 경우 메탄올 생성의 주요 대사는 생물 전기 화학 반응을 통해 산화 전극에서 메탄올을 산화시킴으로써 방출되는 이산화탄소와 수소를 사용하는 hydrogenotrophic methanogenesis다.