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Chestnut내과피를 이용한 수용액중 중금속의 흡착제거에 관한 연구
박성은,고건일,김재백 圓光大學校 藥品硏究所 1990 藥品硏究所報 Vol.5 No.1
Adsorption of metal ions(Cd^2+, Pb^2+) in water by atomic absorption spectrophotometer. Rate of adsorption by the endocarp of chestnut was evaluated under the condition of variation in temperature, shaking time, solution pH, adsorbent amount, and metal ion concentration. The results are as follows: 1. Adsorption rate of Cd^2+, Pb^2+ in water by the endocarp coarse powder of chestnut was each 55∼90%, 22∼99% in the concentration of each Cd^2+ 2.5∼20μg/ml, Pb^2+ 2.5∼200μg/ml. 2. Endocarp coarse powder of chestnut has greater adsorbability than water extracted sample, i.e., 2.2 times in Cd^2+, 2.5 times in Pb^2+. 3. Adsorption of Cd^2+, Pb^2+ by the endocarp coarse powder of chestnut roughly following Freundlich isotherms in the concentration range of each Cd^2+∼20μg/ml, Pb^2+ 2.5∼200μg/ml.
김인주,박창언,윤복현,김일수 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-
According to the rising of national economic level, domestic consumption of vegetables having high additive values is increased continuously due to increased consumption of meat in last decade. These vegetables are produced almost in this country and are limited to import from neighbor countries in due of high transportation expenses for storing in refrigerated container. It is very important to mechanize the harvest work, forming more than 30% for their production cost, in order to cultivate variable vegetables at the same time according to their harvesting seasons. In this state its former harvest methods, with using of human power or semi-automatic harvest, caused to increase their production cost due to high labor cost and low working efficiency.
Extrusion 가공처리 계분사료의 첨가수준이 재래산양의 육성능력, 영양소 소화율 및 체조성에 미치는 영향
김창혁,라창식,고병대,박재인,임광철,신종서 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.6
본 시험은 계분의 가축 사료화의 일환으로 계분, 옥수수 및 타피오카를 50:30:20의 비율로 혼합하여 extrusion 가공한 계분사료(EPM)의 급여수준이 반추가축의 증체량, 사료요구율, 영양소 소화율 및 도체성적에 미치는 영향을 구명하기 위하여 평균체중이 10㎏ 내외의 한국재래산양 수컷 15두를 이용하여 EPM을 시험사료중에 각각 0, 10, 20, 30 및 40%씩 첨가 급여하여 6주간 사양시험을 실시하였다. Extrusion 가공처리 계분사료의 수분, 조지방 및 조섬유 함량는 extrusion 가공처리 전보다 각각 10.8, 0.2 및 0.5% 감소하였고, NFE는 1.7% 증가하였다. 사료섭취량은 대조구의 596.1g/day에 비해 EPM 20 및 30% 첨가구에서 각각 620.6 및 698.5g/day로 증가하는 경향을 보였으나, 40% 첨가구에서는 590.7g/day로 감소하였다. 일당 증체량은 대조구에서 119.8g으로 EPM 10, 20 및 30% 첨가구의 96.8, 98.3 및 108.2g/day와 거의 유사한 수준을 보였으나 EPM 40% 첨가구에서는 78.3g/day로 대조구에 비해 유의적으로 낮았다(p<0.05). 사료요구율은 대조구(5.01)에 비하여 EPM 첨가수준이 증가할수록 높아지는 경향을 보였으며, 특히 EPM 40% 첨가구에서는 대조구에 비해 현저하게 높았다(p<0.05). 건물 및 조단백질 소화율은 각 처리간에 큰 차이는 인정되지 않았으나, 조섬유 소화율은 대조구의 63.7%에 비해 EPM 10, 20, 30 및 40% 첨가구에서 각각 65.3, 67.5, 70.4 및 71.8%로 증가하는 경향을 보였다. 조지방 소화율은 EPM 40% 첨가구에서 68.5%로 대조구에 비해 유의적으로 낮았고(p<0.05). 다른 EMP 첨가구에서는 거의 유사한 수준을 나타내었다. 도체율은 대조구의 49.7%에 비해 EPM 10, 20, 30 및 40% 첨가구에서 각각 49.7, 48.3, 47.8 및 45.2로 감소하는 경향을 나타내었다. 산양고기중의 지방 함량은 대조구의 16.3%에 비해 EPM 첨가구 모두에서 유의적으로 감소하였다(p<0.05). This experiment was conducted to investigate the effects of supplementary level of extruded poultry manure, corn and tapioca mixture (EPM) on growth performance, nutrient digestibility and body composition in korean native goats. Total 15 heads of Korean native goats (10 ㎏ B.W.) were randomly assigned into five treatment groups (EPM 10, 20, 30, 40% and control.) and feeding trial was done for six weeks with ad libitum. Protein level of the extrudate with poultry manure was directly proportional to corn supplements rate. NFE and Ca content in EPM also were functional of the tapioca supplements levels. Daily body weight gain was significantly (p<0.05) reduced in EPM 40% group, but no significant differences were observed in other groups. Concentrate feed intake was high in EPM 10 to 30% groups, compared to the control. Feed conversion ratio also was relatively higher in EPM groups than in the control. As EPM level increased, dry matter digestibility was proportionally decreased, but that of crude protein was enhanced. Dressed carcass percentage was significantly (p<0.05) reduced with the increase of EPM level, showing control 49.7, EPM 10% 49.8, EPM 20% 48.3, EPM 30% 47.9 and EPM 40% 45.2, respectively.
Pak, K.,Shin, M. J.,Hwang, S. J.,Shin, J. H.,Shin, H. K.,Kim, S. J.,Kim, I. J. Elsevier Science B.V., Amsterdam. 2016 Molecular imaging and biology Vol.18 No.5
<P>Electrodiagnostic studies can obtain information 2 or 3 weeks after an acute nerve injury. Previous studies have shown increased glucose metabolism in denervated muscles 1 week after injury using 2-deoxy-2-[F-18]fluoro-D-glucose ([F-18]FDG) positron emission tomography (PET). Therefore, this study aimed to evaluate the changes in glucose metabolism in denervated muscles using serial monitoring by [F-18]FDG PET scans. Denervation was induced in eight male Sprague-Dawley rats (aged 7 weeks old) weighing 200-250 g. The right legs of the rats were denervated by resecting the sciatic nerve in the thigh after the initial skin incision. Two rats were sacrificed 1 and 10 weeks after denervation. Skeletal muscles (gastrocnemius and tibialis anterior) were excised from both the right and left legs of the rats. Staining with hematoxylin and eosin, glucose transporter (GLUT)-1, GLUT-4, and hexokinase II was undertaken. PET/computed tomography (CT) scans were performed on the six remaining rats a total of five times at 1, 2, 5, 8, and 10 weeks after denervation. Regions of interest were drawn on integrated PET/CT images to measure the degree of [F-18]FDG uptake in the right and left lower leg muscles. Target-to-background ratios (TBRs) were calculated by dividing the FDG uptake of the lower leg muscles by that of the upper leg muscles. The TBRs of the denervated muscles were higher than those of the control muscles at both 1 (6.84 +/- 1.98 vs. 1.18 +/- 0.11, p = 0.009) and 2 (4.10 +/- 2.05 vs. 1.86 +/- 0.73, p = 0.0374) weeks after denervation. After 5 (2.18 +/- 0.78 vs. 1.35 +/- 0.47, p = 0.1489), 8 (1.76 +/- 0.18 vs. 1.69 +/- 0.18, p = 0.5127), and 10 (1.76 +/- 0.52 vs. 1.56 +/- 0.37, p = 0.5637) weeks, the difference in the TBRs between the denervated and controls became non-significant. [F-18]FDG PET can visualize increased glucose metabolism in a denervated muscle early as 1 week after injury. Therefore, PET could be adopted as a noninvasive imaging modality for acute nerve injuries. In addition, [F-18]FDG PET may help to understand the role of the nervous system in the control of peripheral tissues.</P>
효소 소화법에 의한 밍크 진피내 Proteoglycan 의 모 성장주기에 따른 함량 변화
박재인 ( J . I . Pak ) 한국축산학회 1998 한국축산학회지 Vol.40 No.5
This study was conducted to investigate the changes in contents of dermal proteoglycans(PGs) on enrymolytic measurement during the hair growth cycle in mink. The results obtained from this research are as follows The total PG contents of mink dermis were high in infants(5 weeks old) and winter anagen(22 weeks old), while the contents were low in summer and winter telogens(15 weeks and 30 weeks). The ratios of PG to collagen content in infants and winter anagen were higher(30 and 25%) than those in summer and winter telogens(16 and 17%). Hyaluronic acid contents of mink dermis were low in infants and anagen, while high in summer and winter telogens. The contents of condroitin sulfate, dermatan sulfate and heparan sulfate of mink dennis were high in infants and anagen, while low in the both telogens. Comparing the PG digestibility by protein resolving enzyme such as pepsin, the PG in the anagen was more hardly dissoved than that in the telogen. The tolerance of PG to protein-denaturant such as guanidine-HCl was higher in the anagen than in the telogen.
Antiarrhythmic Potential of Mesenchymal Stem Cell Is Modulated by Hypoxic Environment
Hwang, H.J.,Chang, W.,Song, B.W.,Song, H.,Cha, M.J.,Kim, I.K.,Lim, S.,Choi, E.J.,Ham, O.,Lee, S.Y.,Shim, J.,Joung, B.,Pak, H.N.,Kim, S.S.,Choi, B.R.,Jang, Y.,Lee, M.H.,Hwang, K.C. Elsevier Biomedical 2012 JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY - Vol.60 No.17
Objectives: The purpose of this study was to evaluate the antiarrhythmic potential of mesenchymal stem cells (MSC) under a different environment. Background: Little is known about how environmental status affects antiarrhythmic potential of MSCs. Methods: To investigate the effect of paracrine factors secreted from MSCs under different circumstances on arrhythmogenicity in rats with myocardial infarction, we injected paracrine media (PM) secreted under hypoxic, normoxic conditions (hypoxic PM and normoxic PM), and MSC into the border zone of infarcted myocardium in rats. Results: We found that the injection of hypoxic PM, but not normoxic PM, markedly restored conduction velocities, suppressed focal activity, and prevented sudden arrhythmic deaths in rats. Underlying this electrophysiological alteration was a decrease in fibrosis, restoration of connexin 43, alleviation of Ca<SUP>2+</SUP> overload, and recovery of Ca<SUP>2+</SUP>-regulatory ion channels and proteins, all of which is supported by proteomic data showing that several paracrine factors including basic fibroblast growth factor, insulinlike growth factor 1, hepatocyte growth factor, and EF-hand domain-containing 2 are potential mediators. When compared with PM, MSC injection did not reduce or prevent arrhythmogenicity, suggesting that the antiarrhythmic or proarrhythmic potential of MSC is mainly dependent on paracrine factors. Conclusions: A hypoxic or normoxic environment surrounding MSC affects the type and properties of the growth factors or cytokines, and these secreted molecules determine the characteristics of the electro-anatomical substrate of the surrounding myocardium.