WK-101 이 생쥐 초기 배아 발생 및 인체 임파구 염색체에 미치는 영향(제2보) : 인체 임파구 염색체에 미치는 영향 The Effect of WK-101 on the Human Lymphocyte Chromosome in vitro

정연태,한두석,박승택,김정중,김재백 圓光大學校 藥品硏究所 1987 藥品硏究所報 Vol.2 No.1

In order to investigate the effect of WK-101 on the human chromosomes, the human chromosomes, the frequency of sister chromatid exchange were examined after cultured human periphral lymphocyte: exposed to WK101 and acetaldehyde. The results obtained were as followings; 1.The acetaldehyde treated groups showed does-dependent increase of the frequency of sister chromatid exchange. 2.The WK-101 treated groups showed similar frequency of sister chromatid exchange as compared with the control group. 3.The WK-101 and acetaldehyde treated groups showed markedly increase of the frequency of sister chromatid exchange. In these case, increase of the frequency of sister chromatid exchange might be due to cytotoxicity by acetaldehyde. From the above results, it is suggested that WK-101 did not show the remarkable effect for the exchange of sister chromatid.

Glycyrrhizin 및 감초엑스제제의 생체이용성에 관한 연구

조내규,김재백 圓光大學校 藥品硏究所 1990 藥品硏究所報 Vol.5 No.1

The pharmacokinetic characteristics of glycyrrhizin was examined using rabbits with i.v. injection of glycyrrhizin. And the present study was carried out to elucidate the difference in the concentration of glycyrrhizin in the rabbit plasma and its absorption after oral administration of glycyrrhizin, licorice exract, licorice exract granule (used Avice)?? as diluent : SEG-A) and commercial licorice extact granule(used lactose and corn starch as diluents : SEG-L). The concentration of glycyrrhizin in the plasma was determined by high performance liquid chromatography. As a result, the plasma decay in the rabbits following i.v. dose 100㎎/㎏ of glycyrrhizin was biphasic (A=1563.11±215.09μg/ml, α=8.07±4.59 hr, B=915.48±97.39μg/ml, β=0.121±0.03 0hr^-1). The time required for a maxium concentration (T_max) of glycyrrhizin was 6hrs after administration of glycyrrhizin. On the other hand, the T_max of giveyrrhizin was about 8 hrs after administration of licorice exract, LEG-A and LEG-L. The C_max of glycyrrhizin in the plasma after administration of glycyrrhizin, licorice exract, SEG-A and LEG-L was each 15.63±3.2, 11.38±1.7, 10.38±2.1 and 9.06±1.9μg/ml. The AUC of glycyrrhizin after the oral administration of glycyrrhizin was almost the same as that of licorice exract and LEG-A. But the AUC of glycyrrhizin after administration of LEG-L was significantly smaller than that of the others.

Scopolamine Hydrobromide 및 l-Hyoscyamine과 制酸劑 및 消化酸素劑와의 相互作用에 관한 硏究

김창영,김재백 圓光大學校 藥品硏究所 1990 藥品硏究所報 Vol.5 No.1

The effects of antacids on the bioavailability of Scopolamine Hydrobromide and l-Hyscyamine were studied in rabbits. The plasma concentration of Scopolamine and l-Hyocsyamine were determined by selected ion monitoring in GC/MS(Scopolamine ; m/z= 138, l-Hyoscyamine; m/z=124). Biphasic elimination was shown by intravenous injection. The AUC of Scopolamine and l-Hyoscyamine was increased as 1.2∼1.3 times by the oral coadministration with antacids. The stability of Scopolamine and l-Hyoscyamine in artificial gastric juicewas increased by antacids, the degradation of Scopolamine and l-Hyoscyamine in the artificial intestinal juice and the optimal pH condition (pH 7.4) was accelerated by Lipase.

Pharmasol 基劑를 使用한 Indomethacin 結晶多形의 直腸吸收에 關한 硏究

이동호,김재백,Lee, D.H. 圓光大學校 藥品硏究所 1986 藥品硏究所報 Vol.1 No.1

Suppositories may be particulary important where a drug cannot be tolerated orally, where the patient cannot swallow, or where the drug may be decomposed or inactivated by the pH and enzyme present in the gastrointestinal tracts Rectal suppositories are used for the introduction of medicaments to be adsorbed into the circulatory system. In order to study on the bioavailability of Acetaminophen rectal suppository. we make two types of suppository; one is a new gelatin capsule (Rectocap) and the other is common suppository (Rectal suppository) After the rectal administration of Rectocap and Rectal suppository, we experiment the serum concentration of Acetaminophen by High Pressure Liquid Chromatography. In serum concentration, the Rectocap is higher and persisted longer than the Rectal suppository This greater serum concentration of Rectocap may be related to a variery of factors including point, the solubility chemical reactivity and the composition in the suppository bases.

담도결찰에 의해 유도된 간섬유화모델에 대한 생물약제학적 연구

한상수,함성호,김기영,손동환,김재백 圓光大學校 藥品硏究所 1992 藥品硏究所報 Vol.7 No.1

The model of liver fibrosis by bile duct ligation was investigated using the aminoterminal procollagen type Ⅲ peptide(PIIINP), total hydroxyproline in the liver and light microscopical histology. Four weeks after bile duct ligation there was an increase in total collagen content of the liver to 199% of initial values accompanied by an increase of serum-PIIINP(385%). Also to assess the potential for utilization related to the pharmacokinetic model of liver fibrosis, We examined the pharmacokinetics of theophylline in the rat of bile duct ligation induced fibrosis, as an indicator drug. An i.v. dose of 10㎎ of aminophylline per ㎏ of body weight was administered, and samples of serum were collected from 0 to 12 hours and were assayed by high performance liquid chromatography. The serum concentration and AUC of theophylline were increased significantly in fibrotic rats compared with those of control. The half-life of theophylline in fibrotic rats was prolonged, with a mean of 17.55±5.53 hours compared to 6.56±1.11 hours in normal rats. The total clearance of theophylline in fibrotic rats was low, averaging 11.19±1.72㎖/㎏/hr in the control rats and the normal rats, respectively. From the above results it was considered that bile duct ligation model was useful for screening method of antifibrotic agents and for pharmacokinetic model of liver fibrosis.

합성 핵산 염기의 피부투과에 관한 연구

이영미,이성희,손동환 圓光大學校 藥品硏究所 1994 藥品硏究所報 Vol.9 No.1

Antisense oligonucleotides have been shown to act as specific inhibitors of gene expression in a variety of in vitro system, Oligonucleotide can be sufficiently protected from degradation in this compartment either by structural modified antisense phosphorothioate oligonucleotide (ASPS) was markedly reduced compared to control. After topical application the in vitro skin transport study and optimal preformulation were investigated for the purpose of effective of ASPS with partially modified ASPS. The cumulative amount penetrated of partially modified ASPS across normal rat skin in PBS containing 6% PF-68 was 0.234 ± 0.041㎍/㎠ and damaged rat skin in PBS containing 6% PF-68 was 1.077 ± 0.301㎍/㎠. The permeation of partially modified ASPS through excised human cadaver and hairless mouse were compared to that of rat skin.

Quercetin이 마우스의 면역반응에 미치는 영향

안영근,박영길,김정훈 원광대학교 식품약품안전성연구소 1991 食品藥品安全性硏究 Vol.4 No.-

Effects of quercetin on the specific and non-specific immune responses were studied in vivo. Quercetin at a dose of 2.5, 5, 10, 20 and 40 ㎎/㎏ were orally administered to ICR male mice once daily for 28 consecutive days. Cuclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5㎎/㎏ 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by humoral and cellular immune reponses and non-specific immune response. The results of this study were summerized as followings: 1. Quercetin significantly decreased the body weight, and introduced the atrophy of liver, spleen and thymus gland dose-dependently, but increased the numbers of white blood cell. 2. Querectin significantly depressed the hemagglutination titer, Arthus reaction and hemolythtic plaque forming cell. 3. Querectin significantly depressed the delayed type hypersensitivity and rosette forming cell. 4. Quercetin at a dose of 2.5, 5 and 40㎎/㎏ significantly depressed phagocytic activity. 5. Quercetin at d dose of 10 and 20 ㎎/㎏ significantly increased natural killer cell activity.

Paraquat 毒性이 Mouse의 免疫反應에 미치는 影響

洪사욱,安榮根,金鍊判 원광대학교 식품약품안전성연구소 1992 食品藥品安全性硏究 Vol.5 No.-

In this paper we have reported that effect of the glycyrrhizin which contained main component in the licorice root on the immunotoxicity of paraquat. The paraquat and glycyrrhizin was administered intraperitoneally by injection in this experiment. Both of treated and untreated mice with paraquat were sensitized and challenged with s-RBC. The immune responses in the untreated mice were evaluated by antibody production, that is, arthus reation, delayed type hypersensitivity (DTH), rosette forming cell and macrophage activity. Although the immune responses in the group treated with paraquat decreased markedly, but in the group treated paraquat with glycyrrhizin significantly restored or potentially reduced humoral and cellular immune response and phagocyte activity.

Effects of Squalene on the Immune Responses in Mice(Ⅰ) : Humorial Immune Responses of Squalene

Ahn, Young Keun,Kim, Joung Hoon 원광대학교 식품약품안전성연구소 1992 食品藥品安全性硏究 Vol.5 No.-

Effects of squalene on humoral immune system in mice were investigated. Squalene exhibited significant increases in the circulating leukocyte counts and relative pleen and thymus weights of the mice. However the relative live weight was slightly decreased. Hemagglutination titers (HA) were signficantly enhanced by squalene while Arthus reaction was not affected. Splenic plaque forming cells (PFC) were also greatly increased by squalene. especially at doses of 50 and 100 ㎎/㎏ of it.

마우스에 있어서 Isoniazid와 Rifampicin의 免疫毒性에 대한 酒淸 및 高麗人蔘의 影響

안영근,김정훈,유항묵,강영세 원광대학교 식품약품안전성연구소 1987 食品藥品安全性硏究 Vol.1 No.-

結核의 一般的인 藥物療法으로 利用되고 있는 Isonicotinic acid hydrazide(INAH)와 Rifampicin의 倂用投與는 結核의 治療效果를 改善시킬 수 있는 反面에 INAH와 Rifampicin의 長期 倂用投與가 肝毒性과 免疫抑制作用을 出現시킨다고 新告된 바 있다. 한편 酒精의 長期間 暴露는 肝毒性과 T-Iymphocyte機能抑制 및 體液性 免疫에 손상을 가져 온다고 알려진 바 있고 高麗人蔘은 細胞性 및 體液性 免疫 修飾作用이 있으며 特히 石油 ether 分劃은 그 作用이 强하여 細胞性 및 體液性 免疫 macrophage의 活性 등을 현저하게 亢進시킨다고 本人 等이 밝힌 바 있다. 本 硏究의 結果 INAH와 Rifampicin倂用與時 이들 藥物에 의한 免疫 毒性에 對하여 酒精 및 高麗人蔘을 倂用하였을 때 INAH와 Rifampicin은 細胞性 및 體液性 免疫을 低下시켰으며 酒精의 倂用投餘는 免疫 毒性을 增加하였으나 高麗人蔘의 倂用投與는 細胞性 및 體液性 免疫과 大食細胞의 活性을 增加시킴을 밝혔다. 1. Rifampicin and INAH exposure decreased relative spleen weight, humoral and cellular immune responses and macrophage activity. 2. Alcohol combined administration significantly increased the cellular immunotoxicity of Rifampicin and INAH. Especially, 4% alcohol combined administration significantly suppressed the cellular immune response and macrophage activity. 3. Generally, Korean giseng extracts reduced of removed the decline of humoral and cellular immune responses and macrophage activity by immunotoxicity of Rifampicin and INAH.