S-143 Spontaneous rupture of hepatic metastasis after curative resection of malignant thymoma

( Ho Jae Kim ),( Se Ju Lee ),( Min Seo Ki ),( Yong Eun Park ),( Seoun Hun Beom ),( Dai Hoon Han ),( Young Nyun Park ),( Jun Yong Park ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Spontaneous hepatic hemorrhage is a rare condition that may be caused by an underlying hepatic tumor, most commonly a hepatocellular carcinoma or a hepatic adenoma. Spontaneous rupture of hepatic metastasis from a thymoma is extremely rare. Here, we report the case of a 62-year-old man with spontaneous rupture of hepatic metastasis from a thymoma that presented as hypovolemic shock. At the first hospital admission, the patient had a 45-mm anterior mediastinal mass that was eventually diagnosed as a type A thymoma. The mass was excised, and the patient was disease-free for 6 years. He experienced sudden-onset right upper quadrant pain and was again admitted to our hospital. We noted large hemoperitoneum with a 10-cm encapsulated mass in S5/8 and a 2.3-cm nodular lesion in the right upper quadrant of the abdomen. He was diagnosed with hepatic metastasis from the thymoma, and he underwent chemotherapy and surgical excision.

Atomoxetine and Fluoxetine Activate AMPK-ACC-CPT1 Pathway in Human SH-SY5Y and U-87 MG Cells

Songhee Jeon(Songhee Jeon),Jeong-Eun Park(Jeong-Eun Park),Young Ho Do(Young Ho Do),Renata Santos(Renata Santos ),Seong Mi Lee(Seong Mi Lee),Bung-Nyun Kim(Bung-Nyun Kim),Jae Hoon Cheong(Jae Hoon Cheong 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.3

Objective Atomoxetine and fluoxetine are psychopharmacologic agents associated with loss of appetite and weight. Adenosine monophosphate-activated protein kinase (AMPK) is the cellular energy sensor that regulate metabolism and energy, being activated by fasting and inhibited by feeding in the hypothalamus. Methods Human brain cell lines (SH-SY5Y and U-87 MG cells) were used to study the outcome of atomoxetine and fluoxetine treatment in the activity of AMPK-acetyl-CoA carboxylase (ACC)- carnitine palmitoyl transferase 1 (CPT1) pathway and upstream regulation by calcium/calmodulin-dependent kinase kinase β (CaMKKβ) using immunoblotting and CPT1 enzymatic activity measures. Results Phosphorylation of AMPK and ACC increased significantly after atomoxetine and fluoxetine treatment in the first 30-60 minutes of treatment in the two cell lines. Activation of AMPK and inhibition of ACC was associated with an increase by 5-fold of mitochondrial CPT1 activity. Although the neuronal isoform CPT1C could be detected by immunoblotting, activity was not changed by the drug treatments. In addition, the increase in phospho-AMPK and phospho-ACC expression induced by atomoxetine was abolished by treatment with STO-609, a CaMKKβ inhibitor, indicating that AMPK-ACC-CPT1 pathway is activated through CaMKKβ phosphorylation. Conclusion These findings indicate that at the cellular level atomoxetine and fluoxetine treatments may activate AMPK-ACC-CPT1 pathways through CaMKKβ in human SH-SY5Y and U-87 MG cells.

Antimicrobial activity and protective effect of Geranium thunbergii against oxidative DNA damage via antioxidant effect

권태형,이수진,박재호,김태완,박정자,박년호,Kwon, Tae-Hyung,Lee, Su-Jin,Park, Jae-Ho,Kim, Taewan,Park, Jung-Ja,Park, Nyun-Ho The Korean Society of Food Preservation 2017 한국식품저장유통학회지 Vol.17 No.5

본 연구에서는 현초의 식품소재 적용과 기능성 소재의 개발을 위해 현초 에틸 아세테이트 분획물을 이용하여 활성산소종의 소거활성과 산화적 DNA 손상 보호효과 및 항균활성에 대해 검증하였다. 현초를 메탄올로 추출하여 얻어진 추출물에 대해 n-hexane, chloroform, ethyl acetate, n-butanol, water의 용매를 이용하여 순차분획을 실시하였고, 얻어진 결과물에 대하여 다양한 항산화 측정 방법을 통하여 항산화 효능을 측정한 결과 에틸 아세테이트 분획물의 경우 DPPH 라디칼 소거능, ABTS 라디칼 소거능 및 환원력에서 효과가 높게 측정 되었으며, $50{\mu}g/mL$의 농도에서 각각 80.88%, 80.12%, 28%를 저해하는 것으로 측정되었다. 이러한 항산화 효능과 함께 산화적 DNA 손상 보호효과를 검증하였고 농도별로 억제하는 경향을 나타냈다. 또한 식품 소재 및 다양한 첨가 소재로 이용하기 위하여 항균활성을 측정하였으며, 에틸 아세테이트 분획물에서 연구에 사용된 모든 균주에 대하여 저해 활성을 보였다. 이러한 활성을 가진 현초 에틸 아세테이트 분획물의 활성물질을 검증하기 위하여 phenolic compound 및 flavonoid 대조군을 이용하여 LC 분석을 하였다. 그 결과 ellagic acid와 gallic aicd가 검출 되었으며 각각 55.14 mg/g, 5.42 mg/g 측정 되었다. 이는 결과적으로 현초는 다양한 식품소재로서의 활용될 수 있으며, 본 논문은 기능성 물질로 활용을 위한 기초자료가 될 것으로 사료 된다. This study aimed to investigate the various biological activities of Geranium thunbergii such as antimicrobial activity and protective effect against oxidative damage. To evaluate its antioxidant and antimicrobial activities, we first performed methanol extraction; this methanol extract was further partitioned using various solvents. And then, its antioxidant activity was measured using various assays including total phenolic content and protection against oxidative DNA damage, and antimicrobial activities were examined using minimum inhibiting concentration (MIC) test, and paper disc method. In addition, high-performance liquid chromatography was performed to analyze the major chemical components of ethyl acetate fraction. The G. thunbergii fraction with ethyl acetate exhibited higher antioxidant and antimicrobial activities than the other fractions. The results showed that G. thunbergii ethyl acetate fraction at $50{\mu}g/mL$ had strong DPPH and ABTS radical scavenging activities of 80.88% and 80.12%, respectively. In addition, the ethyl acetate fraction protected DNA from the oxidative damage induced by ferrous ion and hydroxyl radicals and showed high antimicrobial activity with diameter of inhibition zones ranging from 13.33 to 15.67 mm. High-performance liquid chromatography analysis revealed the major phenolic compounds of G. thunbergii to be ellagic acid and gallic acid. These results suggest that G. thunbergii might protect DNA against oxidative stress induced by reactive oxygen species and can be utilized as a natural source of antioxidant and antimicrobial agent in the food industry.

若年者의 卵巢腫瘍

朴在錫,全相年,洪君植,鄭奇俊,李台鎬 慶北大學校 醫科大學 1970 慶北醫大誌 Vol.11 No.1

Ovarian tumors in children present difficult problems in diagnosis and management. Clinicians has difficult problems in distinguishing the functional varients from the pathologic aberrations which make up ovarian tumors in childhood and in distinguishing benign or malignant neoplasms. The authors undertook this study because of clinical survey of juvenile ovarian tumors as observed 40 cases. The results obtained are as follows: 1) The incidence of ovarian tumors under age of 20(1960-1968) was 5.79% of all ovarian tumors. 2) The incidence of benign ovarian cystadenoma under age of 20 was dermoid cyst 43.5%, mucinous cyst 26%, serous cyst 19.5%. 3) Age distribution of juvenile ovarian tumors was between 9-20 years. The tumors occured frequently between 18-20 years. 4) The incidence of malignant ovarian tumors was about 10%. 5) Among the subjective symtpoms complained of by the patient, about 80% noticed abdominal mass, 15% complained of G-I troubles. 6) As to the objective signs among the patients, about 97% noticed abdominal mass. Abdominal tenderness was observed in 27%. 7) Benign ovarian tumors were observed 44.4% in right ovary, 38.8% in left and 16.6% in bilateral ovaries. We can see the greate roccurence of tumors in right ovary in benign ovarian tumors. 8) The size of benign and malignant ovarian tumors were nearly over adult fist size if the lutein cyst was excluded. The mucinous cysts and serous cysts generally were the largest. 9) The incidence of pedicle torsion of all ovarian tumors was 22.4%. 10) The treatment for benign ovarian tumors of 36 cases was unilateral salpingo-oophorectomy in 30 cases and bilateral salpingo-oophorectomy in 6 cases, and for malignant was surgical primarily and the principle of it was total hysterectomy with bilateral salpingo-oophorectomy with or without omentectomy, Abjuvant treatment, X-ray irradiation was applied to all malignant ovarian tumors.

Long-term outcomes of simple crossover stenting from the left main to the left anterior descending coronary artery

( Ho Myung Lee ),( Chang Wook Nam ),( Yun Kyeong Cho ),( Hyuck Jun Yoon ),( Hyoung Seob Park ),( Hyung Seop Kim ),( In Sung Chung ),( Yun Seok Heo ),( Seung Ho Hur ),( Yoon Nyun Kim ),( Kwon Bae Kim ) 대한내과학회 2014 The Korean Journal of Internal Medicine Vol.29 No.5

Background/Aims: Although complex bifurcation stenting in patients with nonleftmain (LM) bifurcation lesions has not yielded better clinical outcomes thansimpler procedures, the utility of complex bifurcation stenting to treat LM bifurcationlesions has not yet been adequately explored. Methods: In the present study, patients who underwent LM-to-left anterior descending(LAD) coronary artery simple crossover stenting to treat significantde novo distal LM or ostial LAD disease, in the absence of angiographically significantostial left circumflex (LCX) coronary artery disease, were consecutivelyenrolled. The frequencies of 3-year major adverse cardiovascular events (MACEs;cardiac death, myocardial infarction, and target lesion revascularization), wereanalyzed. Results: Of 105 eligible consecutive patients, only 12 (11.4%) required additionalprocedures to treat ostial LCX disease after main vessel stenting. The mean percentagediameter of ostial LCX stenosis increased from 22.5% ± 15.2% to 32.3% ±16.3% (p < 0.001) after LM-to-LAD simple crossover stenting. The 3-year incidenceof MACEs was 9.7% (cardiac death 2.2%; myocardial infarction 2.2%; target lesionrevascularization 8.6%), and that of stent thrombosis 1.1%. Of seven cases (7.5%)requiring restenosis, pure ostial LCX-related repeat revascularization was requiredby only two. Conclusions: Simple crossover LM-to-LAD stenting without opening of a struton the LCX ostium was associated with acceptable long-term clinical outcomes.

Expression of Recombinant Human Bone Morphogenetic Protein 2 (rhBMP2) in Silkworm Pupae and Cells.

Ho Sun SUNG,Jung-Hee WOO,Nyun-Ho PARK,Sun Mee HONG 한국생물공학회 2013 한국생물공학회 학술대회 Vol.2013 No.4

한국인 주의력결핍 과잉행동장애와 도파민 D5 수용체 유전자 다형성의 관련성 : 가족기반 연구 및 환자-대조군 연구

박태원,김붕년,임명호,유희정,강대희,조수철,유일한,조은정,백소영,배치운,서영주 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

Objectives : Recent genetic studies have suggested a preferential transmission of the Dopamine D5 receptor gene (DRD5) 148bp marker allele. The aim of this study is to test the association between DRD5 and ADHD. Methods : 106 Korean children with ADHD and their parents were analyzed using the transmission disequilibrium test (TDT) and haplotype-based haplotype relative risk test (HHRR). And also the ADHD children were compared with 212 ageand gender matched normal controls. Results : We found the evidence for an association of short alleles of DRD5 dinucleotide repeat polymorphism in both Case-control and family based studyies. Additionally, we observed some evidence for biased transmission of allele 152 bp and 144 bp. Conclusion : Our results lend credence to the notion that the relationship between ADHD and DRD5 is complex. The number of cases and informative transmissions in our study were small, therefore it would be premature to make any conclusions Con-ceming the role of DRD5 in ADHD. Further work is needed to support these findings.

若年者의 卵巢腫瘍

朴在錫,全相年,洪君植,鄭奇俊,李台鎬 慶北大學校 醫科大學 1970 慶北醫大雜誌 Vol.11 No.1

내시경적 용종절제술로 진단된 Gastritis Cystica Polyposa 2예

박상진,박인서,박영년,정재복,정준표,송시영,김호근 대한소화기내시경학회 1993 Clinical Endoscopy Vol.13 No.3

Gastritis cystica polyposa(GCP) is a polypoid cystic lesion showing all the histological features of the hyperplastic polyps and the cysts penetrating through the mucularis mucosae. Most reported GCP lesions were developed at gastraenterostomy stomas. It is rare that GCP develops without history of previous gastroenterostomy. In 1990, however, Kim et al reported a GCP presenting as a submucosal tume-like lesion in a 69-year-old man. Recently, we had experienced two consecutive cases of GCP diagnosed by endoscopic polypectomy. The first case was 49-year-old housewife without history of previous gastric surgery. Esophagogastroduodenoscopy(EGD) showed a 2.0x1.5x1.5cm sized lobulated polypoid lesion with a broad stalk on the anterior wall of the distal antrum. The second case was 45-year-old woman without history of previous gastric surgery. EGD revealed a 1.0 x 1.0 x l.0 cm sized polypoid lesion with a pedicle on the greater curvature aspect of the upper body and two duodenal ulcers with pseudodiverticulum formation. These lesions were endoscopically polypectomized and diagnosed as GCP by the histologic examination.

SALL4 Expression in Hepatocellular Carcinomas Is Associated with EpCAM-Positivity and a Poor Prognosis

Park, Hyunjin,Lee, Hyejung,Seo, An Na,Cho, Jai Young,Choi, Young Rok,Yoon, Yoo-Seok,Han, Ho-Seong,Park, Young Nyun,Kim, Haeryoung The Korean Society of Pathologists and the Korean 2015 Journal of Pathology and Translational Medicine Vol.49 No.5

<P><B>Background:</B></P><P>There is increasing interest in hepatocellular carcinomas (HCC) expressing “stemness”-related markers, as they have been associated with aggressive behavior and poor prognosis. In this study, we investigated the usefulness of Sal-like protein 4 (SALL4), a recently proposed candidate marker of “stemness.”</P><P><B>Methods:</B></P><P>Immunohistochemical stains were performed for SALL4, K19, and epithelial cellular adhesion molecule (EpCAM) on tissue microarrays constructed from 190 surgically resected HCCs, and the results were correlated with the clinicopathological features and patient survival data.</P><P><B>Results:</B></P><P>Nuclear SALL4 expression was observed in 39/190 HCCs (20.5%), while K19 and EpCAM were expressed in 30 (15.9%) and 92 (48.7%) HCCs, respectively. The nuclear expression was generally weak, punctate or clumped. SALL4 expression was significantly associated with a poor overall survival compared to SALL4-negative HCCs (p = .014) compared to SALL4-negative HCCs. On multivariate analysis adjusted for tumor size, multiplicity, vascular invasion, and pathological tumor stage, SALL4 remained as a significant independent predictor of decreased overall survival (p= .004). SALL4 expression was positively correlated with EpCAM expression (p = .013) but not with K19 expression. HCCs that expressed both SALL4 and EpCAM were associated with significantly decreased overall survival, compared to those cases which were negative for both of these markers (p = .031).</P><P><B>Conclusions:</B></P><P>Although SALL4 expression was not significantly correlated with other clinicopathological parameters suggestive of tumor aggressiveness, SALL4 expression was an independent predictor of poor overall survival in human HCCs, and was also positively correlated with EpCAM expression.</P>