Research Articles : Trichoderma asperellum Chi42 Genes Encode Chitinase

( Nguyen Hoang Loc ),( Hoang Tan Quang ),( Nguyen Bao Hung ),( Nguyen Duc Huy ),( Truong Thi Bich Phuong ),( Tran Thi Thu Ha ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.

Analytical Asymptotic Expressions for the Green’s Function of the Electron in a Single-Level Quantum Dot at the Kondo and the Fano Resonances

Nguyen Van Hieu,Nguyen Bich Ha,V. P. Gerdt,O. Chuluunbaatar,A. A. Gusev,Yu. G. Palii,Nguyen Van Hop 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.6

The Kondo and the Fano resonances in the two-point Green’s function of the single-level quantum dot were found and investigated in many previous works by means of different numerical calculation methods. In this report, we present a derivation of the analytical forms of the resonance terms in the expression of the two-point Green’s function. For that purpose, the system of Dyson equations for the two-point nonequilibrium Green’s functions in the complex-time Keldysh formalism was established to the second order in the tunneling coupling constants with the mean field approximation for the statistical averages of the products of four electron creation and destruction operators. This system of Dyson equations was solved exactly by means of the computer algebra and the analytical expressions for the resonance terms are derived. The conditions for the existence of the Kondo or the Fano resonances were found.

Four New Compounds Isolated from the Aerial Part of <i>Belamcanda chinensis</i> (L.) and Their Effect on Vascular Smooth Muscle Cell (VSMC) Proliferation

Ha, Do Thi,Binh, Bui Thi,Thu, Nguyen Thi,Bich Thu, Nguyen Thi,Thanh Tung, Pham Ha,Oh, Won Keun Pharmaceutical Society of Japan 2019 Chemical & pharmaceutical bulletin Vol. No.

Critical Factors Affecting Employers' Satisfaction with Accounting Graduates in Hanoi

NGUYEN, Hoan,NGUYEN, Lien Thi Bich,NGUYEN, Hong Nhung,LE, Thanh Ha,DO, Duc Tai Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.8

In recent years, many firms have built a good recruitment policy, focusing on the requirements set for candidates to meet the employers' satisfaction; they often have certain requirements for each accounting job's position. The study aims to identify and measure factors affecting the employers' satisfaction with accounting graduates in Hanoi, the important locus of firms' labor force. We conducted a questionnaire consisting of 16 observation variables with a 5-point Likert scale. Independent variables were measured from 1 "without effect" to 5 "strongly". Based on the literature review and results of interviews, a total of 150 questionnaires were sent to participants; 135 of them met the standards and were subject to be analyzed. The results of Cronbach's alpha, Exploratory Factor Analysis (EFA) and Multiple Regression Analysis (MRA) identify three main determinants influencing the employers' satisfaction with accounting graduates in Hanoi, including students' experience before graduating (SEG), reputation of universities (RU), and university's recruitment support policy (RSP). Based on the findings, some recommendations have been proposed to help universities design training programs for creating better satisfactions for employers in the future. On this basis, the authors propose a number of recommendations to improve the employers' satisfaction with accounting graduates in Hanoi.

Zanthoxylum rhetsa Stem Bark Extract Inhibits LPS-induced COX-2 and iNOS expression in RAW 264.7 Cells via the NF-kB Inactivation

Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Nguyen Viet Than,Thipthaviphone Soulinho,Nguyen Hai Nam,Tran Thi Phuong,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4

The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-a (TNF-a), the mRNA expression levels of pro-inflammatory cytokines, including TNF-a and IL-1b, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-kB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.

Screening of Vietnamese Medicinal Plants for Cytotoxic Activity

Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Tran Viet Hung,Tran Thi Hien,Yim Namhui,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.1

Thirty-two methanol extracts of thirty-one Vietnamese medicinal plants were evaluated for the cytotoxic activity against five human cancer cell lines, including A549, MCF-7, HT 1080, Huh-7, and HepG2. Of these, the nine extracts of Acanthopanax trifoliatus (4), Acanthopanax gracilistylus (5), Siegesbeckia orientalis (10), Betula alnoides (11), Passiflora edulis (18), Zanthoxylum simulans (leaf, 23), Adenosma caeruleum (26), Solanum verbascifolium (29), and Alpinia malaccensis (31), exhibited high potent cytotoxic activity showing a certain degree of selectivity against the different cell types, with IC50 values ranging from 2.1 to 3.8 mg/mL.

Trichoderma asperellum Chi42 Genes Encode Chitinase

Loc, Nguyen Hoang,Quang, Hoang Tan,Hung, Nguyen Bao,Huy, Nguyen Duc,Phuong, Truong Thi Bich,Ha, Tran Thi Thu The Korean Society of Mycology 2011 Mycobiology Vol.39 No.3

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.

A Taguchi approach with electron-beam irradiation to optimize the efficiency of removing enrofloxacin from aqueous media

Hong Thi Bich Truong,Hiep Nghia Bui,Hieu Trung Nguyen,Thanh-Luu Pham,Duy Ngoc Nguyen,Yuan-Shing Perng,Linh Thi My Lam,Thi-Dieu-Hien Vo,Van-Truc Nguyen,Ha Manh Bui 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.4

Electron-beam (EB) irradiation was employed to degrade enrofloxacin (ENR) in an aqueous solution. Thealgal growth inhibition test revealed that ENR exhibited low toxicity against the cyanobacterium Arthrospira sp., with anEC50-96 h value of 5.17mg/L. The Taguchi design also involved finding the best optimum for ENR treatment using EB. Results revealed that the high-efficiency removal of ENR in an aqueous solution was approximately 98.53% under theoptimum conditions of an absorbed dose of 5 kGy, a pH of 5.0, and an initial ENR concentration of 10 mg/L and anH2O2 concentration of 2mM. The ERR degradation under a couple of EB irradiation and H2O2 followed pseudo-firstorderkinetics, with an R2 of ~0.970. The major degradation pathways of ENR were suggested by density functional theory,natural bond orbital calculations, and liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis. Lifecycle assessment (LCA) was also performed to evaluate the impact of the EB on removing ENR; the industrial processwas designed based on laboratory tests aimed with the ReCiPe tool. The obtained results indicated that energy consumptionand H2O2 affect environmental impacts with order human health, ecology systems, and natural resource. The LCAalso proved that EB could be a green and efficient method for eliminating pharmaceutical contaminants in water.

General Theory of Decoherence of Qubit Systems in the Born-Markov Approximation

R. Joynt,Nguyen Bich Ha,Nguyen Hung Son,Ho Cong Son,Nguyen Dang Vu,Nguyen Van Hieu 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.1

The general theory of the decoherence of a two-level system as a qubit and a four-level system as a two-qubit system based on the Born-Markov approximation is presented. The derived formulae in the case of the two- level system are applied to two examples of the qubit: a system of electron states in a double quantum dot as a charge qubit and that of Zeeman split electron states in a quantum dot as a spin qubit. A generalization to the study of the decoherence of n-qubit systems is discussed.

한국 소비자의 온라인 식품구매 결정요인과 식품 공급사슬 다각화에 관한 연구

정재은(Jae-Eun Chung),Nguyen Thi Thu Ha(Thi Thu Ha Nguyen),Nguyen Thi Bich Loan(Thi Bich Loan Nguyen),문희철(Hee-Cheol Moon) 중앙대학교 한국전자무역연구소 2020 전자무역연구 Vol.18 No.1

연구목적: 최근 온라인 공급업체들은 마케팅 방법과 유통방법의 다각화를 통하여 소비자 만족도 향상에 기여하고 있으며, 이에 따라 온라인 식품시장이 급격히 성장하고 있다. 본 연구는 이러한 온라인 식품시장에서 한국 소비자의 식품구매 결정요인을 실증적으로 분석해 식품 공급사슬 현황에 대한 시의 적절한 시사점을 제공하고자 한다. 논문구성/논리: 연구의 논리성 및 시의 적절성을 제시하기 위해 본 연구에서는 이론적인 연구배경과 선행연구에 대해 제시한 후 이를 참고로 연구모형과 연구가설을 구축하였다. 연구가설 검증에는 SPSS 통계패키지와 AMOS 프로그램이 활용되었으며, 요인의 타당성과 신뢰성 및 측정모형의 적합도를 검증한 후 가설검증을 이행하여 실증분석 결과의 신뢰도를 확보하였다. 본 연구의 차별성을 확보한 조절효과 검증에는 다집단분석 방법이 활용되었다. 결과: 본 연구의 실증분석 결과에 의하면 온라인 공급업자가 제공하는 식료품과 관련정보의 높은 신뢰도는 온라인 식품구매 방법에 대한 소비자의 태도에 대해 긍정적인 영향을 미치는 것으로 나타났다. 또한 온라인 식품 구매 방법에 대해 소비자가 유용하다고 인지할수록 소비자가 온라인 식품구매에 대해 긍정적인 태도를 가지게 되며, 이는 소비자의 구매하고자 하는 의도에도 직접적인 영향을 미치는 것으로 분석되었다. 마지막으로 식료품의 구분에 따른 조절효과가 유의한 것으로 검증된 것에서 제품구분에 따른 소비자의 구매 결정에 대한 영향요인이 다르며, 이는 한국 식품시장의 공급사슬이 다변화, 다각화되고 있어 다른 시각적 접근의 필요성을 제시하였다. 독창성/가치: 본 연구는 최근 소비가 급증하고 있는 한국의 온라인 식품시장에 대해 새로운 시각적 접근의 필요성을 제시하고 식료제품의 구분을 조절적으로 분석해 소비자의 결정요인이 다르게 작용하고 있음을 실증적으로 제시하였다. Purpose: Recently, online suppliers have contributed to improving customer satisfaction through diversification of marketing methods and distribution methods. As a result, the online food market is growing rapidly. This study empirically analyzed the determinants of food purchase of Korean consumers in this online food market, and tried to provide timely implications for the current Korean food supply chain status. Composition/Logic: In order to suggest the logic and timeliness of the study, this study first described the theoretical background and previous studies. Based on this, the research model and hypotheses were constructed. To test the hypothesis, SPSS statistical package and AMOS program were used. After verifying the validity and reliability of the factors and the model fit, the hypothesis verification was conducted to secure the reliability of the empirical analysis results. The multi-group SEM analysis was used to verify the moderating effect, which secured the differentiation of this study. Findings: According to the empirical results of this study, the high reliability of food products and related information provided by online suppliers has a positive effect on consumers attitudes toward online food purchase methods. In addition, as consumers perceive the usefulness of online food purchase method, consumers have a positive attitude toward online food purchase, which has a direct effect on their purchase intention also. Lastly, it was analyzed that the moderating effect of food products classification was significant. This suggests the need for a different approach as the supply chain of the Korean online food market is diversifying. Originality/Value: This study suggested the necessity of a new approach to Korea s online food market, which has recently been increasing rapidly, and empirically suggested that consumers determinants are working differently by analyzing the moderate effect of food products classification.