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Converting a Host Receptor into Sustained Intranasal Virucides against SARS-CoV-2 Using Nanodiscs
Jaehyeon HWANG,Wonbeom PARK,Soomin KIM,SeungJoo KIM,Suhyun KIM,Nayoon CHOI,Eunkhang PARK,Hwanju KIM,Mina KIM,Hyunjoo CHOO,Soyun CHOI,MinKyeom KIM,YeonJin CHO,Dae-Hyuk KWEON 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.10
화장품 브랜드 CMF 디자인에 나타난 브랜드 아이덴티티 색채 적용에 관한 분석
김나윤(Nayoon Kim) 한국색채학회 2018 한국색채학회 학술대회 Vol.2018 No.5
본 연구에서는 화장품 시장의 브랜드 경쟁 속에서 기업들이 가지는 브랜드 아이덴티티의 유지 및 발전에 대한 한계점을 해결하고자 CMF 색채마케팅을 하나의 중요 전략으로 브랜드별 CMF 디자인의 BI색채 및 색채 적용에 관한 현황을 분석하였다. 연구의 진행 방법은 선정된 4개의 국내 및 해외 화장품 브랜드를 대상으로 홈페이지 및 컨셉을 바탕으로 BI색채를 분석하고 Facial-Makeup 제품을 카테고리에 따라 분류한 뒤 KSCP(Korean Standard Color Palette)를 통해 먼셀 색값과 sRGB색값을 도출, KS A 0011을 기준으로 요약하였다. I.R.I 형용사 이미지 스케일로 브랜드별 이미지를 찾아내고 NCS색체계로 브랜드 색값을 요약하였다. 본 연구는 브랜드 색채를 단편적으로 사용하는 방법이 아닌 다양한 색상과 톤으로 브랜드 이미지를 표현하기 위한 방안을 마련하고자 한다.
백교림,Nayoon Hur,Min Jhi Kim,Jun-Ho Choe,Jung Han Kim,Jee Soo Kim 대한갑상선-내분비외과학회 2019 The Koreran journal of Endocrine Surgery Vol.19 No.4
Purpose: Energy devices, such as Harmonic Focus Plus (Ethicon; ultrasonic scalpel; Harmonic) and Ligasure Small Jaw (Covidien; bipolar sealer; Ligasure) are widely used in thyroid surgery as substitutes for Bovie (electrocautery) and sutures. They reduce operation time and peri-operative complications. Thunderbeat Open Fine Jaw (Olympus; Thunderbeat) is a recently developed energy device, which combines the functions of both ultrasonic scalpel and bipolar sealer. We prospectively evaluated the safety and feasibility of Thunderbeat during open thyroidectomy compared with Harmonic and Ligasure. Methods: From September to November 2016, patients with papillary thyroid cancer or benign thyroid disease who underwent unilateral or bilateral thyroidectomy at Samsung Medical Center were enrolled. Thyroidectomies were performed by a single surgeon who specializes in endocrine surgery and is experienced in using energy devices. The patients were randomly allocated to one of the 3 groups according to the energy device used during the operation: Harmonic, Ligasure, and Thunderbeat group. Results: No significant differences were detected among the 3 groups in intra-operative bleeding, drainage amount, incidence of transient hypocalcemia, and transient vocal cord palsy. The surgical time for thyroidectomy in the T group was shorter than in the other 2 groups (P=0.031). However, the H group showed reduced surgical time for central lymph node dissection. Conclusion: Among the 3 energy devices, Thunderbeat and Harmonic shortened the surgical time for thyroidectomy and central lymph node dissection, respectively. However, each energy device has its own strengths and weaknesses, and we recommend the selection of devices based on the respective features.
Development of Fc-conjugated Nanodisc for Enhanced Virucidal Efficacy against Influenza A Viruses
KyeongWon KIM,Jaehyeon HWANG,Younghun JUNG,Jeong Hyeon YOON,Soomin KIM,Nayoon CHOI,Dae-Hyuk KWEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Several studies have conducted on neutralizing antibodies (NAbs) against influenza A viruses causing loss of life and economic damage. However, these virustatic antibodies lacking virucidal activity showed limited antiviral potency. Here, we develop a nanodisc providing the immunoglobulins with virucidal efficacy by rupturing the viral envelope, resulting in enhancing the antiviral effects. Mechanistically, the nanodiscs carrying Fc-binding peptides bind to NAbs targeting stem region of hemagglutinin are co-endocytosed into host cells. The nanodisc-based nanotransformer (NT) perforate the viral membranes at low pH in the endosome. Administration of the NT, especially with pegylated nanotransformer, reduce morbidity and mortality compared with the NAbs in mouse models. Our results suggest a new class adjuvant of applicable to other neutralizing antibodies and convalescent plasma therapy that confer virucidal potency by antibody-dependent perforation. In conclusion, the membrane nanostructure gives another prospective for concepts of antibody conjugated molecules.
Producing Full-length Botulinum Neurotoxin (BoNT) Using Protein Conjugation
Minju KIM,Jihwan CHUN,Wonbeom PARK,SeungJoo KIM,Yong Hwan LEE,Nayoon CHOI,Dae-Hyuk KWEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Botulinum neurotoxin is the most lethal biological toxin in nature. Regarding its mechanism of action, BoNT has been greatly favored not only for cosmetic purposes, but also for disease treatment. Conventionally, production of BoNT relied on the tedious cultivation of C. botulinum, which yielded BoNT complex containing NAPs (nontoxic neurotoxin-associated proteins). However, the existence of NAPs is detrimental since 1) it greatly increases the molecular weight of the final product (about 900 kDa), and 2) it may reduce the efficacy of BoNT by facilitating resistance development. To resolve such issues, we designed a novel approach to produce BoNT by expressing separated fragments of BoNT (LC-HN and HC domains) followed by protein conjugation. Each domain was fused with conjugative protein fragments and the conjugation efficiency under various conditions including molar ratio, temperature, and time. As a result, we confirmed that the maximal reaction efficiency is achieved when equivalent amount of protein fragments were combined and incubated at 37℃ for 10 min. Moreover, specific sequences of LC-HN were almost completely cleaved by thrombin treatment, confirming the formation of a disulfide bond between the separated LC and HN domains. In this study, we suggest that BoNT can be produced more safely and efficiently through a novel approach employing protein conjugation and specific cleavage.