Transcriptome profiling of olive flounder responses under acute and chronic heat stress

Woo‑Jin Kim,이규빈,Dain Lee,Hyun‑Chul Kim,Bo‑Hye Nam,Hyungtaek Jung,이선주,Kyunghwan Kim 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background The olive founder (Paralichthys olivaceus) is a saltwater fsh, which is valuable to the economy. The olive founder strives to adapt to environmental stressors through physiological, biochemical, and transcriptional responses. The rise in water temperature threatens the growth, development, reproduction, and survival of olive founder. Each organ in the olive founder can diferentially respond to heat stress. Objectives The purpose of this study is to investigate organ-specifc transcriptional changes in olive founder tissues during heat stress. Methods In this study, transcriptome dynamics of the gill, liver, and muscle of olive founder to acute or chronic heat stress were investigated. Results Principal component analysis plotting revealed that the transcriptome of each organ is quite separated. K-means clustering, gene ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed the diferential transcriptome responses of each organ to heat stress. Heat stress commonly afects the pathways involved in the correct protein folding, DNA repair, and cell cycle. Conclusion Our results may provide a valuable molecular basis of heat acclimation in fshes.

제6차와 제7차 교육과정에서 초등학교 과학 교과서 삽화 비교 분석 : Centered on the 3rd Year Class of the Elementary School

남철우,권영길 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.1

This study was intended to examine the difference and the suggestion through comparative analysing the illustrations in the 3rd elementary science textbooks of the 6th with the 7th curriculum. The findings of this research were as follows. (1) Compared with the 6th curriculum, 448 more illustrations are presented in the 7th curriculum science textbook and the teacher's teaching techniques should be, therefore, improved the using ability of the illustration in the science education. (2) Although more than 96% of the illustrations are those of photographs and pictures in the 6th curriculum, those in the 7th consist of many kinds of the illustrations-photographs, picture, diagrams and cartoons. Especially, the more frequent introductions of the cartoons in the textbook give familiarity to the students, and it suggests that the textbooks were constructed in consideration of the student's intellectual development. (3) The roles of illustrations in the 6th and the 7th curriculum are mostly guidance for experiments and the supply of the learning materials. but considering that the learning motivation is very important in the teaching-learning process. the percentage of the illustrations in accordance with the motivations must be increased, (4) As the result of the analysis of illustration in the each domains, in the 6th curriculum there are the most numbers of illustrations in the biology domain and the least in the physics domain. But contrastively in the 7th curriculum there are the most in the energy and the least in physics, and namely those are distributed widely. (5) Analysing the kinds of illustrations in the each domains, in the both sixth and seventh curriculum the photographs are used in the physics mostly, and it is, therefore, interpreted because of the reason that the more realistic material supply should be needed. (6) According to the roles of illustrations in the each domains, there are the many illustrations for guidance of the experiments in the both curriculum, and contrastively in the biology part and the earth science, the many illustrations are introduced for material supply. In this viewpoint it is known that the illustrations of the physics domains and the energy are constructed for experiment, and those of the biology domain and earth science for investigation and observation of the given materials. (7) the average numbers of illustrations to be taught in a period or in a page are introduced more twice in the sixth text-book than the seventh. It is, naturally, concluded that the teachers' training process for using the illustrations is needed urgently.

産業勤勞者의 健康增進 및 行態에 관한 關聯要因分析

姜永佑,南喆鉉 韓國保健敎育學會 1997 보건교육건강증진학회지 Vol.14 No.2

From September 2, 1995 through October 31, this questionnaire was made by the 1,200 industrial workers who work for 15 enterprises of 5 types of business. And it was for helping to devise a policy and to develop a program for industrial workers' health promotion by understanding the consciousness behavior level of industrial workers in our country and the related factors which are under the influence of it and health promotion behavior. The summary and conclusions are as follows. 1. In health promotion score level by related factors, the health diagmosis level score was 7.37(31.9/100) of the perfect score 9, nutrition level score was 7.00(77.8/100), health education behavior level score was 6.00(66.7/100), exercise behavior level score was 6.01(66.8/100), occupational disease knowledge level score was 6.00(66.7/100). 2. Health diagnosis behavior level was significantly high when the age is older, when the occupation term is longer, when the economic status is better. And wjem tje satosfoed degree for vocational aptitude, working environment, and the education contents. 3. Nutrition (dietary habit) status level was high in men, in the age group of 40 over, in the group of having their spouse, in the group of being paid over one million won a month, in the upper economic classes(P<0.001). It was also high in graduates middle school and in daytime workers(P<0.05). 4. Health education behavior level was high in the older ahe hroup, in a single life (separation by death, divouce, separation) and in the longer occupation term(P<0.001). 5. Exercises behavior level was high in men, in the workers who are paid 500∼990 thousand won a month, in the better economic classes(P<0.01). 6. Knowledge level on an occupational disease was high in men, in the older age group, in the group of having a spouse, in the workers who are paid 500∼990 thousand won a month, in group of having a longer occupation term, and in the residents living not in a large city(P<0.01). 7. When health status was higher, health promotion behavior level, health diagnosis (P<0.001), nutrition(P<0.05), health education behavior(P<0.05), exercise behavior (P<0.01)and the knowledge level on an occupation disease was high. 8. The main factors which are under the influence on the degree of practicing healthy life were the level of knowledge and behavior, sex, his/her health status, and the satisfied degree of working environment. These variables could explain it 18.0%. 9. The factors which are under the influence on health promotion behavior and behavior levels were the variables of the satisfied degerr of education contents, sex, health knowledge, economic status, health status, occupation terms, monthly income, working tiredness. These variables could explain it 21.3%. By these results, it is important for industrial workers' health promotion to level up the health diagnosis behavior, dietary habit considering nutrition, behavior on health education, behavior for exercise, and knowledge on an occupational disease. Especially we should develop the proper program considered sex, health status, satisfied degree of working environment and education contents, economic status, eccupation terms, knowledge on health, and behavior level. Because health promotion business gies in gear with productivity promotion.

Li^+이온이 주입된 D_2O 얼음의 열자극 발광 현상에 관한 연구

박영우,황정남,이철주 연세대학교 자연과학연구소 1987 學術論文集 Vol.19 No.-

저온에서 편극시킨 탈이온수 얼음의 열자극 편극 소거 전류(Thermally Stimulated Depolarization Current ; TSDC)를 77K∼250K 범위에서 측정하여 Li^+ 이온이 주입된 D_2O 얼음의 이온공간전하 열자극 전류(Ionic Space Charge Thermocurrent ; ITC)와 열자극 발광(Thermoluminescence ; TL)을 측정하였다. D_2O 얼음의 TSDC인 경우는 173K에서 heterocharge가 나오고 200K에서 homocharge가 나온다. 이 때 heterocharge는 1차 kinetic임을 알았고, initial rise법으로 구한 활성화에너지는 0.16eV이다. D_2O 얼음의 heterocharge는 bulk에 의한 효과이고, homocharge는 공간전하에 의한 TSDC임을 확인하기 위하여, 가속기를 이용해 Li^+이온을 주입시킨 D_2O 얼음의 TL을 조사해본 결과 114K(A-peak), 180K(C-peak)에서 3개의 TL peak가 형성되는데 각각의 활성화에너지는 0.03eV, 0.15eV 및 0.29eV이다. 이중에 B-peak의 활성화에너지 값과 거의 일치하여 그 결과로 부터 B-peak의 근원이 이온공간전하에 의한 쌍극자 재정렬임을 최초로 확인하였다. Thermally stimulated depolarization current(TSDC) has been measured in distilled and deionized water ice in the temperature range of 77∼250K at a constant heating rate, and the ionic space charge thermocurrent(ITC) and thermoluminescence(TL) has been measured from Li^+ion implanted D_2O ice. In the case of TSDC of D_2O ice, the heterocharge give rise to 173K, while the homocharge give rise to 200K TSDC peak. The heterocharge peak TSDC is found to be 1st kinetic and that the activation energy is o.16eV using the initial rise method. In order to investigate that the heterocharge is due to the polarization in the D_2O ice and the homocharge due to the ionic space charge, TL curve from Li^+ion implanted D_2O ice and the homocharge due to the ionic space charge, TL curve from Li^+ion implanted D_2O ice shows three peaks at 114K, 148K and 183K. The activation energy for these TL peaks are 0.03eV, 0.15eV and 0.29eV, respectively. The activation energy for B-peak(0.16eV) is coincide with that of ITC peak which means that both TL peak and ITC peak are caused by the dipole reorientation due to ionic space charge. This approach to clarifies for the first time to my knowledge.

농촌지역사회의 보건교육 요구도에 관한 연구

김종우,남철현,김성우 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.2

At the opening of a new millennium and a new century, health promotion and education services in Korea are in the early developmental stage. The National Health Promotion Act legislated in 1995 was a milestone for initiating a national and local health promotion program in Korea. And since then local governments and health centers have been developing and providing health promotion program for the community populations. The short history of health promotion and education in Korea has meant that local governments and health centers have a limited experience and organizational capacity for health promotion and education planing and practice. This study was attempted to measure health education need of rural community and to analyze the factors for health education need assessment. Surveyors interviewed 1250 subjects randomly selected. Subjects were 2.17% of men and women in Changnyung county and older then 20 years old. Data were collected from April 17, 2000 through April 27, 2000. The questionnaire consisted of general characteristics, health educational experiences, health educational method, health educational content and health educational needs for rural community residents. The questions on the health educational needs of content consist of 36 questions in 8 fields. The statistical methods used for the analysis were χ2 -test, t-test, F-ratio and ANOVA using SPSS program. In conclusion, despite more needs to the respondents who are in the low education level and socioeconomic state, in the old age, in the low health knowledge, they required less health education. To enjoy a more healthy life after more community residents actively understand and are interested in health education and health promotion, we certainly require a designed and systemic health education. The resources of health department in Korea are limited and the investment involved in health promotion and health edcation is severely reduced. Particularly this situation is more severe in the rural community. To select and perform an effective health education methods that the nature and reality of the rural community are considered, we'll use the resources to invest in health promotion affairs as effectively as possible and then they will take the responsibility of healthy community.

관상동맥 성형술이 QT 간격분산에 미치는 영향

신이철,김윤년,현대우,허승호,박남의 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.11

B_(2)O_(3)첨가에의한 LNT(Li_(1/2)Nd_(1/2)TiO_(3))-CT(CaTiO_(3)) 세라믹의 저온 소결 및 마이크로파 유전특성

이찬우,최명호,김남철 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

The effect of B_(2)O_(3) addition on the microstructures and the microwave dielectric properties of 0.5LNT(Li_(1/2)Nd_(1/2)TiO_(3)) - 0.5CT(CaTiO_(3)) Ceramics have been investigated. It is found that B_(2)O_(3)(up to 5wt%) can significantly improve the density and dielectric properties of 0.5LNT(Li_(1/2)Nd_(1/2)TiO_(3)) - 0.5CT(CaTiO_(3)) Ceramics. 0.5LNT-0.5CT Ceramics with additives could be sintered to a density higher then 95% at 900℃, due to the liquid phase effect of B_(2)O_(3) addition, as observed by Scanning electron microscopy(SEM). A secondary pahse was not observed at the level of 5 ~ 10wt% B_(2)O_(3) addition. The microwave dielectric properties of 0.5LNT(Li_(1/2)Nd_(1/2)TiO_(3)) - 0.5CT(CaTiO_(3)) with 5wt%B_(2)O_(3) were as follows : ε_(r) = 85, Qxf_(0) = 2,500 and Tcf = -9ppm/℃, sintered at 900℃/2hr.

TMAH에 의한 이방성 식각을 이용한 3차원 자기센서의 제작

정우철,남태철 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

This paper present an anisotropic etching in TMAH technique used in the fabrication of three-dimensional magnetic field vector sensor based on angled Hall plate structure. This sensor design relies on simultaneously detecting all magnetic field vector components using Hall plates that are imbedded into the silicon [111] sloped-surface of bulk micromachined cavity by the anisotropic etching of [100] silicon. The fabricated Hall elements has relatively improved sensitivity compare to convensional Hall elements for three-dimensional magnetic field sensing. The product sensitivity of 547V/AT at the supply current of 1.0mA has been achieved.

도시지역 한방의료이용자의 질병양상 조사연구

강영우,조태현,김병하,남철현,조남춘,안창수,이재홍 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

To provide the basic data for improving of curative measures in oriental medicine and for developing of health education and preventive program after understanding of classification of diseases in oriental medicine users, questionnaire interview was carried out with city dwellers, over 20 years old, selected randomly in big cities from 2nd Oct. to 11th Nov. 1995. The results were summarried as follows: 1. In the general characteristics of subjects, the highest portion of each part was 61.2% of female, 25.5% of thirties, 28.5% of high school graduate, 63.3% of middle class and 11.8% of lower class in economic status. 31.9% was housewife, 73.5% was merried and 55.1%, 38.1% came from big or small cities each. 2. By 21 classification of diseases, 19.7% was blood disorders, 17.6% was pregnancy & delivery complications and 15.2% was endorine & nutritional disorders. 3. As for age 22.6% of endocrine & nutritional disorders in twenties, 20.8%, 28.1%, 27.8% of blood & defence mechanism disorders in thirties, forties, sixties each and 24.9% of pregnancy & delivery complications in fifties were the higher than the other groups. 4. By educational level, 47.8%, 30.5% of blood & defence mechanism disorders in uneducated and middle school graduate were the higher than the other groups. 5. As for occupation, 20.8%, 32.9% of blood & defence mechanism disorders in housewives and sales each, were the higher than the other groups. 6. By economic status, 20.2% of blood & defence mechanism disorders in middle and high class, were the higher than the other groups. 7. By marital status, 21.3% of endocrine & nutritional disorders in singles, 22.0%, 25.7% of blood disorders in having partners, were the higher than the other groups. 8. As for living areas, 18.2% of pregnancy & delivery complications in big city dwellers, 21.3% of endocrine & nutritional disorders in town dwellers were the higher than the other groups. The distribution of diseases in oriental medicine users wes very different by chracteristics according the results. So It's important and essential project to improve curative technics and to develop.

임의의 횟수의 휴가를 갖는 M^x/G/1 및 Geo^x/G/1 대기행렬의 분석

채경철,김남기,이호우 한국경영과학회 2002 韓國經營科學會誌 Vol.27 No.2

By using the arrival time approach of Chase et al. [6], we derive various performance measures including the queue length distributions (in PGFs) and the waiting time distributions (in LST and PGF) for both M^x/G/1 and Geo^x/G/1 queueing systems, both under the assumption that the server, when it becomes idle, takes multiple vacations up to a random maximum number. This is an extension of both Choudhury[7] and Zhang and Tian[11]. A few mistakes in Zhang and Tian are correscied and meaningful interpretations are supplemented.