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Na Liu,Qiujin Zhu,Xuefeng Zeng,Bowen Yang,Meilian Liang,Li Deng,Laping He,Cai Liang,Ruping Zhang,Juan Zhou 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.3
The study aims to determine the optimum sterilization rate and water activity of Chinese traditional smoke-cured bacon product (Larou) in the preservation with natural coating solution. With the response surface methodology (RSM), we analyzed 3 factors of processing conditions (the concentration of lysozyme, concentration of sodium alginate, and concentration of chitosan) and 2 response factors (sterilization rate and water activity). Sterilization rate and water activity of Larou were largely affected by the concentration of lysozyme, concentration of sodium alginate, and concentration of chitosan. The final optimum concentrations of lysozyme, sodium alginate, and chitosan were 0.09, 1.40, and 1.60% and realized the high sterilization rate. Water activity of sliced Larou was significantly correlated with the sterilization rate. Low-field nuclear magnetic resonance analysis verified the optimum processing conditions. The coating resulted in 99.69% rate of reducing bacteria after 30-day storage. The data of the total number of colony, peroxidation value, moisture content, pH, and sensory evaluation provided the theoretical basis for extending the shelf life of Chinese traditional smoke-cured bacon product (Larou) with natural coating solution.
Na-na Li,Jian-liang Lu,Xin-qiang Zheng,Yue-rong Liang 한국차학회 2015 한국차학회지 Vol.- No.S
Albino tea cultivars have precious white shoots or yellow shoots and are widely cultivated in China to produce high quality green tea with fresh taste and high aroma, including temperature-sensitive albino mutants and light-sensitive albino mutants. Physiological and biochemical indicators showed that the levels of pigments, total catechins and caffeine decreased, the content of total amino acids, especially theanine increased, and the chloroplast structure were abnormal in albino leaves; molecular studies displayed that expression of genes encoding chloroplast structure and chlorophyll synthesis, such as light harvesting chlorophyll a/b-binding protein, glutamyl-tRNA reductase, Mg-chelatase H subunit, S-adenosylmethionine synthetase and glutamine synthetase, suppressed in albino period
Susceptibility Loci Associations with Prostate Cancer Risk in Northern Chinese Men
Wang, Na-Na,Xu, Yong,Yang, Kuo,Wei, Dong,Zhang, Yao-Guang,Liu, Ming,Shi, Xiao-Hong,Liang, Si-Ying,Sun, Liang,Zhu, Xiao-Quan,Yang, Yi-Ge,Tang, Lei,Zhao, Cheng-Xiao,Wang, Xin,Chen, Xin,Hui, Juan,Zhang, Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Background: KLK3 gene products, like human prostate-specific antigen (PSA), are important biomarkers in the clinical diagnosis of prostate cancer (PCa). G protein-coupled receptor RFX6, C2orf43 and FOXP4 signaling plays important roles in the development of PCa. However, associations of these genes with PCa in northern Chinese men remain to be detailed. This study aimed to investigate their impact on occurrence and level of malignancy. Methods: All subjects were from Beijing and Tianjin, including 266 cases with prostate cancer and 288 normal individuals as controls. We evaluated associations between clinical covariates (age at diagnosis, prostate specific antigen, Gleason score, tumor stage and aggressive) and 6 candidate PCa risk loci, genotyped by PCR- high resolution melting curve and sequencing methods. Results: Case-control analysis of allelic frequency of PCa associated with PCa showed that one of the 6 candidate risk loci, rs339331 in the RFX6 gene, was associated with reduced risk of prostate cancer (odds ratio (OR) = 0.73, 95% confidence interval (CI) =0.57-0.94, P = 0.013) in northern Chinese men. In addition, subjects with CX (CC+TC) genotypes had a decreased risk for prostrate cancer compared to those carrying the TT homozygote (OR =0.64, 95% CI = 0.45- 0.90, P = 0.008). The TT genotype of 13q22 (rs9600079, T) was associated with tumor stage (P=0.044, OR=2.34, 95% CI=0.94-5.87). Other SNPs were not significantly associated with clinical covariates in prostate cancer (P > 0.05). Conclusions. rs339331 in the RFX6 gene may be associated with prostate cancer as a susceptibility locus in northern Chinese men.
Zhao, Cheng-Xiao,Liu, Ming,Xu, Yong,Yang, Kuo,Wei, Dong,Shi, Xiao-Hong,Yang, Fan,Zhang, Yao-Guang,Wang, Xin,Liang, Si-Ying,Zhao, Fan,Zhang, Yu-Rong,Wang, Na-Na,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
Background: Evidence supporting an association between the 8q24 rs4242382-A polymorphism and prostate cancer (PCa) risk has been reported in North American and Europe populations, though data from Asian populations remain limited. We therefore investigated this association by clinical detection in China, and meta-analysis in Asian, Caucasian and African-American populations. Materials and Methods: Blood samples and clinical information were collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=335) and from age-matched normal controls (n=347). The 8q24 (rs4242382) gene polymorphism was genotyped by polymerase chain reaction-high-resolution melting analysis. We initially analyzed the associations between the risk allele and PCa and clinical covariates. A meta-analysis was then performed using genotyping data from a total of 1,793 PCa cases and 1,864 controls from our study and previously published studies in American and European populations, to determine the association between PCa and risk genotype. Results: The incidence of the risk allele was higher in PCa cases than controls (0.222 vs 0.140, $P=7.3{\times}10^{-5}$), suggesting that the 8q24 rs4242382-A polymorphism was associated with PCa risk in Chinese men. The genotypes in subjects were in accordance with a dominant genetic model (ORadj=2.03, 95%CI: 1.42-2.91, $Padj=1.1{\times}10^{-4}$). Presence of the risk allele rs4242382-A at 8q24 was also associated with clinical covariates including age at diagnosis ${\geq}65$ years, prostate specific antigen >10 ng/ml, Gleason score <8, tumor stage and aggressive PCa, compared with the non-risk genotype ($P=4.6{\times}10^{-5}-3.0{\times}10^{-2}$). Meta-analysis confirmed the association between 8q24 rs4242382-A polymorphism and PCa risk (OR=1.62, 95%CI: 1.39-1.88, $P=1.0{\times}10^{-5}$) across Asian, Caucasian and African American populations. Conclusions: The replicated data suggest that the 8q24 rs4242382-A variation might be associated with increased PCa susceptibility in Asian, Caucasian and African American populations. These results imply that this polymorphism may be a useful risk biomarker for PCa in multi-ethnic populations.
( Na Young Yoon ),( Cheng Liang Xie ),( Kil Bo Shim ),( Yeon Kye Kim ),( Doo Seog Lee ),( Ho Dong Yoon ) 한국수산과학회(구 한국수산학회) 2011 Fisheries and Aquatic Sciences Vol.14 No.4
The antioxidant and cholinesterase inhibitory activities of methanol, pretanol, and acetone extracts of Eupausia superba were investigated and their bioactivities compared. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2`-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid] (ABTS+) radical-scavenging activities and reducing power assays were used to determine antioxidant activities, and Ellman`s colorimetric methods were applied to evaluate cholinesterase inhibitory activity. Although all extracts were positive, Acetone extract of E. superba showed the highest activities. However, these showed moderate or no inhibitory activity against butyrylcholinesterase. Moreover, the total carotenoid contents of the organic solvent extracts followed the same order as their antioxidant and acetylcholinesterase inhibitory activities. These results suggest that E. superba is a potential source of natural antioxidants and cholinesterase inhibitors.
Liang, Hui,Na, Young Mee,Chun, In Sung,Kwon, Soon Sik,Lee, Young-A,Jung, Ok-Sang Chemical Society of Japan 2007 Bulletin of the Chemical Society of Japan Vol.80 No.5
<P>Dinuclear 1,2-semiquinonato/catecholatocobalt complexes containing 1,1,4,7,10,10-hexamethyltriethylenetetramine (hmdeta) as a potential tetradentate N<SUB>4</SUB> coligand, [Co<SUB>2</SUB>(hmteta)(dbbq)<SUB>4</SUB>], (dbbq = 3,5- and 3,6-di-<I>tert</I>-butyl-1,2-benzoquinone (3,5-dbbq and 3,6-dbbq)) were synthesized and characterized. The crystal structures proved that [Co<SUB>2</SUB>(hmteta)(3,6-dbbq)<SUB>4</SUB>]·2C<SUB>6</SUB>H<SUB>5</SUB>CH<SUB>3</SUB> ([3,6]) exists as low spin [(3,6-dbsq)(3,6-dbcat)Co<SUP>III</SUP>(hmteta)Co<SUP>III</SUP>(3,6-dbsq)(3,6-dbcat)] (3,6-dbsq = 3,6-di-<I>tert</I>-butyl-1,2-semiquinonato; 3,6-dbcat = 3,6-di-<I>tert</I>-butylcatecholato), while [Co<SUB>2</SUB>(hmteta)(3,5-dbbq)<SUB>4</SUB>]·C<SUB>6</SUB>H<SUB>5</SUB>CH<SUB>3</SUB> ([3,5]) approximates to [(3,5-dbsq)<SUB>2</SUB>Co<SUP>II</SUP>(hmteta)Co<SUP>II</SUP>(3,5-dbsq)<SUB>2</SUB>] in the solid state at ambient temperature. On the basis of the effective magnetic moments, the [Co<SUP>III</SUP>] → [Co<SUP>II</SUP>] conversion of [3,6] underwent a relatively abrupt transition around 330 K while that of [3,5] occurs in a wide range of temperature. Electronic absorption spectra showed that [3,6] shifts predominantly to [Co<SUP>III</SUP>] whereas [3,5] shifts to [Co<SUP>II</SUP>] valence tautomer in solution at room temperature. The charge distribution of [3,6] exhibited significant solvent effects at room temperature. These prominent features between [3,5] and [3,6] appeared to be associated with difference between delicate electronic and steric effects of the two dbbq ligands.</P>
Statistical-based evaluation of design codes for circular concrete-filled steel tube columns
Na Li,Yi-Yan Lu,Shan Li,Hong-Jun Liang 국제구조공학회 2015 Steel and Composite Structures, An International J Vol.18 No.2
This study addresses the load capacity prediction of circular concrete-filled steel tube (CFST) columns under axial compression using current design codes. Design methods given in the Chinese code CECS 28:2012 (2012), American code AISC 360-10 (2010) and EC4 (2004) are presented and described briefly. A wide range of experimental data of 353 CFST columns is used to evaluate the applicability of CECS 28:2012 in calculating the strength of circular CFST columns. AISC 360-10 and EC4 (2004) are also compared with the test results. The comparisons indicate that all three codes give conservative predictions for both short and long CFST columns. The effects of concrete strength, steel strength and diameter-to-thickness ratio on the accuracy of prediction according to CECS 28:2012 are discussed, which indicate a possibility of extending the limitations on the material strengths and diameter-to-thickness ratio to higher values. A revised equation for slenderness reduction factor in CECS 28:2012 is given.
( Na Young Yoon ),( Sang Hoon Lee ),( Kil Bo Shim ),( Chi Won Lim ),( Moon Hee Lee ),( Hyun Ah Cho ),( Cheng Liang Xie ) 한국수산과학회(구 한국수산학회) 2013 Fisheries and Aquatic Sciences Vol.16 No.1
To assess the feasibility of phlorotannins from Eisenia bicyclis as cancer chemopreventative agents, we tested whether they induced quinone reductase (QR) in Hepa1c1c7 cells. The ethyl acetate (EtOAc) soluble fraction obtained from E. bicyclis exhibited a QR induction activity in Hepa1c1c7 cells. Successive column chromatography of the active EtOAc fraction resulted in the isolation of four phlorotannins. Their structures were elucidated using one- and two-dimensional nuclear magnetic resonance spectroscopic techniques and characterized as phloroglucinol (1), dioxinodehydroeckol (2), dieckol (3), and fucofuroeckol-A (4). Among these compounds, fucofuroeckol-A (4) showed moderate QR induction activity, and dioxinodehydroeckol (2) exhibited potent QR induction potency with 2.05 ± 0.04 fold induction at a concentration of 50 μM compared to the dimethyl sulfoxide solvent-treated control cells. However, phloroglucinol (1) and dieckol (3) exerted no detectable QR induction activity in Hepa1c1c7 cells. These results suggest that dioxinodehydroeckol could serve as a useful cancer chemopreventive chemical.
Na Sun,Tong Yu,Jun-Xing Zhao,Yaogui Sun,Junbing Jiang,Zhi-Bian Duan,WenkuiWang,Yuan-Liang Hu,Hai-Min Lei,HongquanLi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
Twenty natural compounds isolated fromtraditional Chinese medicines were examined for theirantiviral activities against PCV2 in vitro. Antiviral activitywas analyzed by the assays of blocking of virus entry,inhibition of virus replication and inactivation of virusparticles in the virus suspension. 50% cytotoxic concentration(CC50) and 50% effective concentration (EC50) for eachcompound were determined by using the MTT method andimmunofluorescence assay (IFA), respectively. The resultsobtained from the inhibition of virus replication andinactivation of virus particles showed that the maximuminhibition rates for matrine and scutellarin were 57 and72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virusentry and inhibition of virus replication demonstrated thatboth aesculetin and arecoline hydrochloride stimulatedPCV2 replication, with the maximum stimulation rate of387.3 and 219.4%. In summary, both matrine and scutellarinhad antiviral activity against PCV2, while aesculetin andarecoline hydrochloride promoted PCV2 infection replicationin vitro.