癩菌檢出에 對한 skin smear와 Nuclepore法의 比較

張明雄,崔大卿,朴成均 大韓癩學會 1980 癩學 會誌 Vol.13 No.1

Ureaplasma urealyticum과 Mycoplasma hominis가 精子 運動性에 미치는 影響

張明雄,白太鉉,崔大卿 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

The human spermatozoal motility was significantly affected by the whole culture solution and slightly less significantly by the microbial cell suspension of Ureaplasma urealyticum but not the microbial metabolites(culture supernatant). The same was observed with the preparation of Mycoplasma hominis in somewhat minor degree.

CFCM 기반 적응 뉴로-퍼지 시스템에 의한 비선형 시스템 모델링

곽근창,김성수,유정웅,전명근 충북대학교 컴퓨터정보통신 연구소 2002 컴퓨터정보통신연구 Vol.10 No.2

본 논문에서는 여러 분야에서 널리 응용되고 있는 적응 뉴로-퍼지 시스템에서의 효과적인 퍼지 규칙 생성 방법을 제안한다. 기존의 입력공간 그리드 분할을 이용한 ANFIS의 규칙 생성에 있어서는 얻어진 규칙의 수가 지수적으로 증가하는 단점이 있다. 이에, 본 연구에서는 조건부적인 퍼지 클러스터링(CFCM)을 이용하여 입·출력 데이터의 특성을 잘 반영할 수 있는 클러스터를 구하고, 퍼지 균등화 방법을 적용하여 출력변수의 소속함수를 자동 생성하도록 하였다. 이렇게 함으로써 적은 규칙 수를 갖으면서도 효율적인 퍼지 규칙을 얻을 수 있도록 하였다. 이들 방법의 유용함을 보이고자 정수장 응집제주입결정 모델링에 적용하여 제안된 방법이 기존의 연구보다 좋은 결과를 보임을 알 수 있었다. In this paper, an efficient fuzzy rule generation scheme for Adaptive Network-based Fuzzy Inference System(ANFIS) using the conditional fuzzy c-means(CFCM) and fuzzy equalization(FE) methods is proposed. Usually, the number of fuzzy rules exponentially increases by applying the grid partitioning of the input space, in conventional ANFIS approaches. Therefore, GFCM clustering method is adopted to render the clusters which represent the given input and output fuzzy data and FE method is used to automatically construct the fuzzy membership functions. From this, one can systematically obtain a small size of fuzzy rules which shows satisfying performance for the given problems. Finally, we applied the proposed method to the nonlinear system modeling problems and obtained a better performance than conventional works.

2차 및 3차 병원에서 분리된 균종들에 대한 항생제 감수성

이숙경,장명웅 고신대학교 의학부 1999 高神大學校 醫學部 論文集 Vol.14 No.1-2

Objective : This study was carried out to compare the isolation rates of antibiotic resistant bacteria between the secondary hospital (B) and the tertiary hospital (P). Materials and Methods : The 3,813 strains of bacteria were isolated from various specimens in the B and P hospital. Antibiotic susceptibility test was performed by disc diffusion method with ampicillin, carbenicillin, cefamandole, cefotaxime, ciprofloxacin, gentamicin, imipenem, ampicillin-sulbactam, trimethoprim-sulfamethoxazole, cephalothin, ceftazidime, piperacillin, amikacin, tobramycin, vancomycin, erythromycin, oxacillin, penicillin, and clindamycin. Result : The major pathogenic bacteria with high isolation rate in both the B and the P hospital were E. coli, K pneumoniae, S. aureus, and P. aeruginosa. The isolation rate of E coli, a resistance to cefamandole, ciprofloxacin, gentamicin, ampicillin-sulbactam, and trimethoprim-sulfamethoxazole was attentively higher in the P hospital than in the B hospital. The isolation rate of K. pneurnoniae, a resistance to ampicillin, carbenicillin, ciprofloxacin, gentamicin, ampicillin-sulbactam, and trimethoprim-sulfamethoxazole was attentively high in the P hospital. The isolation rate of S. aureus, a resistance to ampicillin-sulbactam, cephalothin, tnmethoprim-sulfamethoxazole, oxacillin, and enicillin was attentively high in the P hospital. The isolation rate of P. aeruginosa, a resistance to gentamicin, imipenem, and amikacin was attentively high in the P hospital. The isolation rate of S. epidermidis, a resistance to ampicillin-sulbactam, cephalothin and trimethoprim-sulfamethoxazole was attentively high in the P hospital. The isolation rate of P. mirabilis, a resistance to cefamandole, cefotaxime, gentamicin, trimethoprim-sulfamethoxazole and cephalothin was attentively high in the P hospital. Conclusion : The isolation frequency of the antibiotic resistant bacteria from the P hospital was higher than that of the B hospital. These results demonstrate that the isolation frequency of the antibiotic resistant bacteria from both B and P hospital showed some difference according to the kinds of antibiotics and bacterial strains. These results attracted our attention to use of antibiotics, and it is urgent to arrange a countermeasure against the abuse of the antibiotic agents.

결핵균 검출의 개선에 관한 연구

백태현,최대경,장명웅 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

Sputum specimen from the patients with active cases of tuberculosis frequently contains so few tubercle bacilli that they are difficult to detect. For the improved detection of tubercle bacilli from sputum specimens, author applied practically filter smear method by using membrane filter. In this experiment, Millipore membrane filters(0.45㎛ in pore size, 25㎜ in diameter) were used to concentrate 208 sputum specimens for detecting tubercle bacilli by microscopic examination. These results were compared with those of direct and centrifugate smears on the same specimens. The results were summarized as follows: 1. Out of 208 sputum specimens, the number of positive specimens by microscopy was 151(72.6%) on direct smears, 172(82.7%) on centrifugate smears and 190(91.3%) on filter smears. 2. Out of 208 sputum specimens, the average Gaffky number was 2.60 on direct smears, 3.64 on centrifugate smears and 5.35 on filter smears. 3. Out of 57 negative specimens by direct smears, 26(45.6%) were positive on centrifugate smears and 39(68.4%) were positive on filter smears and the average Gaffky number was 1.69 and 2.79, respectively. 4. Out of 29 specimens which are Gaffky number 1 by direct smears, the average Gaffky number was 2.62 on centrifugate smears and 3.45 on filter smears. From the above results, filter smear method was more sensitive than centrifugate smear method. Furthermore, the procedure was more simple and rapid that of centrifugate smear. Therefore, it may be a useful primary bacteriological technique to detect tubercle bacilli from the patients of pulmonary tuberculosis.

대퇴 전자간 골절에서 금속정 고정술 시행결과 : 감미정과 근위 대퇴정의 비교 Comparision Gamma Nail and proximal Femoral Nail

고상봉,조명래,김태훈,장일웅 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적: 대퇴 전자간 골절 환자에서 감마정과 근위 대퇴정으로 내고정을 시행하고 방사선학적, 임상적 결과 및 합병증을 비교하여 각각의 장단점을 분석하고자 하였다. 대상 및 방법: 1999년 1월부터 2002년 2월까지 대퇴 전자간 골절로 감마정과 근위 대퇴정을 이용하여 고정한 환자 중 방사선학적 및 임상적으로 6개월 이상 추시가 가능하였던 63예를 대상으로 하였으며 감마정군이 31예, 근위 대퇴정군이 32예이었다. 연령, 골절의 형태와 안정성에 따른 분류, 동반 질환, 골다공증의 정도, 환자의 활동 능력 등이 양 군에서 차이가 없었다. 평가방법으로는 두 군 사이의 수술시간, 출혈량, 안정정복 획득의 감입 정도 등을 비교하였다. 결과: 수술시간은 감마정군은 평균 103±40분, 근위 대퇴정군에서 101±28분으로 통계적인의미는 없었으며 (p=0.801), 수술 중 출혈량은 근위 대퇴정군에서 적은 경향을 보였으나 통계적인 의미는 없었다(p=0.601). 술 후 골절의 안정성 획득 여부는 감마정군 30%, 근위 대퇴정군 31%에서 안정 정복을 얻어 사용기구에 따른 고정의 안정성 획득에는 유의한 차이가 없었으며(p=0.934), 술 후 내반 고정의 빈도와 골절부위의 1.28단계의 보행능력 감소를, 근위 대퇴정군에서 평균 0.86단계의 보행능력 감소를 보여 역시 통계적으로 의미있는 차이는 없었다(p=0.383). 결론: 대퇴 전자간 골절치료에 있어서 임상적 및 방사선학적인 결과에서는 의미있는 차이는 없었으나, 근위 대퇴 골수정이 수술시간, 출혈량 등의 수술 수기의 측면에서 조금 더 우수한 경향을 보였다. Purpose: To evaluate the clinical and radiologic results of the fracture using the Proximal Femoral Nail(PFN) or Gamma nail in patient with the intertrochanteric fracture. Materials and Methods: From January 1999 to February 2002, we reviewed 63 patients of intertrochanteric fractures including 31 patients managed with Gamma nail and 32 patients with PFN. In variate analysis of age, the pattern and stability of fracture, bone density(Singh index), preoperative patient activity, there was no difference between two groups. We evaluated the operation time, blood loss, stable reduction rate and varus reduction rate as operation-related factors, and radiologically investigated union, time, the frequency of delayed union and nonunion, failure of fixation, status of the fracture line and complications. Functional results were evaluated by the possibility of early ambulation, ambulation ability at last follow up, and the pre and postoperative difference of ambulatory ability. Results: Mean operation time was 103±40 minutes in Gamma nail group 101±28 minutes in PFN group. It was not statistically significant(p=0.801). Even though intraoperative blood loss was less in PFN group, it was significant(p=0.601). Postoperative stable reduction rate was 31% in Gamma nail group and 30% in PFN group(p=0.934), suggesting not significant. Postoperative varus reduction and impacted amount of the fracture ling was not significant. Delayed union developed in one case of Gamma nail group and 2 cases of PFN group. Mean loss of ambulation ability was 1.28 grade in Gamma nail group and 0.86 grade in PFN(p=0.383). Statistical analysis was performed with the use of Student T test and Chi-square test. Analyses resulting in a p value of <0.05 were considered to show significance. Conclusion: Generally, the radiologic and clinical results of intertrochanteric fractures were comparable for the PFN group and Gamma nail group. Even though it was not statistically significant, PFN group had superior results in operation time blood than Gamma nail group for the fixation of the patients with interchanteric fracture.

Phytol과 들미나리추출물이 Sarcoma 180마우스의 T Subset에 미치는 효과

류태형,박건영,장명웅,이숙희,선우양일,김광혁 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

본 연구는 녹황색채소류에서 추출되어 항암효과를 나타내는 활성물질로 보고 되어 있는 phytol과 들미나리추출물을 sarcoma 180마우스에 주사한 후 적출한 비장세포내 T 임프구와 T subset, 그리고 asialo ?? 세포를 정량하여 다음과 같은 결과를 얻었다. 1) 종양 마우스에 phytol을 투여하였을 때 비장세포내의 T cell과 T-subset은 종양세포이식에 의하여 상승된 치를 더욱 증가시켰다. 그러나 들미나리추출물의 경우는 대동소이하였다. 2) Asialo ?? 세포는 종양마우스에 phytol이나 들미나리추출물을 주사하였을 때 모두 상승하였으며 정상마우스에 phytol을 투여하였을 때도 대조군에 비하여 상승했지만 들미나리추출물을 작용시켰을 때는 저하되었다. 3) ??/?? 세포비는 종양마우스에 phytol을 주사하였을 때 감소를 보였지만 정상 마우스에 투여하였을 때는 더욱 크게 낮아졌다. 그러나 들미나리추출물을 정상마우스에 투여하였을때는 크게 감소하던 것이 종양마우스에 적요 시켰을때는 증가현상을 보였다. 이상의 결과로 미루어 볼 때 phytol이나 들미나리추출물은 종양마우스에서 작용자세포인 자연살해세포(natural killer cell)의 활성인자로서 작용할 가능성이 높을 것으로 사료된다. Several studies have shown that phytol isolated from perilla leaf and small water dropwort(Oenanthe javanica(BL)D.C, wild type)extract reveal antitumor activities. In the present study we investigated the effect of phytol and the small water dropwort extract on the T subset in the sarcoma 180-transplanted mice in order to elucidate the immunological mechanism of antitumor activity of these substances. The results obtained from the experiment were as follows : When phytol was injected into the sarcoma-180 transplanted mice(control), the levels of T cell and T subset by inoculation of the tumor cells were enhanced, but that of T cells in case of small water dropwort extract was similar to the control. Asialo ?? cells were increased when phytol or small water dropwort extract was injected into tumor-transplanted mice. In normal mice the number of asialo ?? cells increased with phytol injection and decreased with small water dropwort extract injection. ??/?? cell ratios were decreased when phytol was injected into tumor-transplanted mice, but increased in case of small water dropwort extract injection. In normal mice the ratios showed large decreases with phytol or small water dropwort extract injection. These results indicate that phytol or small water dropwort extract can activate the proliferation of natural killer cells that are effector cells in tumor-bearing mice.

産婦人科 患者에서 分離된 Ureaplasma urealyticum의 生物學的 性狀

梁熙燦,崔大卿,張明雄,白太鉉,朴鼎圭 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

Isolation rates of Ureaplasma urealyticum from vaginal swab of 216 patients, 105 prostitutes and 100 college females was investigated. Also, minimal inhibitory concentration of isolated strains about tetracycline and minocycline was studied. The results were summarized as follows; 1. Out of 70 vaginitis patients, isolation rates of Ureaplasma urealyticum and Mycoplasma hominis were 34.3% and 15.7%, respectively. 2. Out of 54 cervicitis patients, isolation rates of Ureplasma urealyticum and Mycoplasma hominis were 37.0% and 11.0%, respectively. 3. Out of 92 other gynecological patients, isolation rates of Ureaplasma urealyticum and mycoplasma hominis were 44.6% and 16.3%, respectively. 4. Out of 105 prostitues, isolation rates of Ureaplasma urealyticum and Mycoplasma hominis were 54.3% and 75.2%, respectively. 5. Out of 100 college students, isolation rates of Ureaplasma urealyticum and Mycoplasma hominis were 23.0% and 7.0% respectively. 6. In the susceptibility patterns of isolated Ureaplasma urealyticum, resistant strains about tetracycline in patients, prostitues and college students were 75.2%, 73.6% and 69.5%, respectively 7. In the susceptibility patterns of isolated Ureaplasma urealyticum, resistant strains about minocycline in patients, prostitues and college students were 91.7%, 87.7% and 73.9%, respectively.

하시모토 병과 동반된 악성 빈혈 1 예

한 호,안명주,최정혜,이창범,이영열,최일영,김인순,이웅수 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.2

Pernicious anemia is the most common cause of vitamin B12 deficiency and is caused by autoimmune destruction of parietal cell containing gastric mucosa region. This disease is significantly associated with autoimmune endocrinopathies and anti-receptor autoimmune disease. We report here a case of 26-year old man who was diagnosed pernicious anemia with high level of autoantibody titer (antithyroglobulin antibody 1:25,600 and antimicromosomal antibody 1:6,400) indicating subclinical Hashimoto's thyroiditis. Since other autoimmune diseases are frequently associated with pernicious anemia even without evidence of clinical symptoms, screening test for the presence of autoimmune disease should be evaluated in these patients.

ANTIBIOTIC SUSCEPTIBILITY TEST AGAINST U. UREALYTICUM ISOLATED FROM CLINICAL SPECIMEN.

Chang, Myung Woong,Kim, Kwang Hyuk,Park, InDal,Bae, Kwang Sung 고신대학교 의학부 1987 高神大學校 醫學部 論文集 Vol.3 No.1

비뇨생식기계 감염환자로부터 분리된 203주의 Ureaplasma urealyticum을 대상으로 oxytetracycline, minocycline 및 josamycin에 대한 감수성 정도를 시험관 희석법에 의한 최저발육 저지 농도로 조사하였다. Oxytetracycline, minocycline 및 josamycin의 최종 발육저지농도의 평균치는 각각 4.0, 8.0 및 0.5 ㎍/㎖였다. Oxytetracycline에 대해서는 분리균주의 11.9%만이 감수성이었고 13.8%는 중등도의 내성균이었으며, 74.3%는 고도 내성균이었다. Minocycline에 대해서는 분리균주의 12.9%만이 감수성이었고, 2.0%는 중등도의 내성균이었으며 85.1%는 고도 내성균이었다. Josamycin에 대해서는 분리균주의 87.7%가 감수성이었으며 12.3%만이 내성균이었다. 분리균주중 10주는 3가지 약제에 모두 내성이었다.