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H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향
정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4
Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.
Ryou, Myung-Hyun,Lee, Dong-Jin,Lee, Je-Nam,Lee, Hong-Kyeong,Seo, Myung-Won,Lee, Hye-Won,Shin, Weon-Ho,Lee, Yong-Min,Choi, Jang-Wook,Park, Jung-Ki The Korean Electrochemical Society 2011 Journal of electrochemical science and technology Vol.2 No.4
A polymer electrolyte was prepared by using polyvinylidenefluoride-co-hexafluoropropylene (PVdF-HFP) or poly(ethylene glycol) dimethacrylate (PEGDMA) as polymer matrices, succinonitrile as an additive, and lithium perchlorate as a lithium salt. Compared to the polymer electrolyte employing PVdF-HFP, the PEGDMA-based polymer electrolyte exhibits substantially superior thermal stability when exposed to high temperatures. Nonetheless, the ionic conductivity of the PEGDMA-based polymer electrolyte was preserved in a wide temperature range between $-20^{\circ}C$ and $80^{\circ}C$.
일별 기상자료를 이용한 농경지 물 수지 및 토양수분 예측모형(AFKAE0.5) 개발
서명철(Myung-Chul Seo),허승오(Seung-Oh Hur),손연규(Yeon-Kyu Sonn),조현숙(Hyeon-Suk Cho),전원태(Weon-Tai Jeon),김민경(Min-Kyeong Kim),김민태(Min-Tae Kim) 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.6
밭 재배면적이 증가하면서 토양내 수분함량에 따른 적정관개가 작물생산성에 중요한 역할을 하기 때문에 손쉽게 농가에서 포장의 토양수분함량을 파악하고 대처하는 것이 중요하다. 본 시험은 농경지에서 간단한 일 기상자료와 관개이력으로 물 수지 및 토양수분함량을 일단위로 추정하기 위하여 AFKAE0.5 토양수분추정모형을 개발하고 이를 검증을 하고자 하였다. 다양한 토양의 특성을 단순화 하기 위해 대상토양을 300mm의 균일한 토양으로 가정하였고 대상 토양지하에서 물 이동을 정의하기 위하여 토양수분장력에 따른 3개의 수분영역을 설정하여 투수량과 상승량을 산출하여 물 수지에 반영하였다. 토양 물수지에서 투입요인으로는 강우, 관개, 수분 상승량을 설정하였고 산출요인으로는 증발산, 유거, 투수량을 설정하여 모형을 개발하였다. 각 요인의 물량 산출을 위한 추정모형은 문헌 및 보고서를 이용하여 작성하였으며 모형을 검증하기 위해 공주지역 고추포장을 대상으로 작부기간 동안 AFKAE0.5 모형을 이용하여 모의하여 물 수지를 산출하였으며 모형의 검증을 위하여 실제 포장에서 층위별 센서를 이용하여 측정한 토양수분함량 변화값을 비교하였다. 대상 포장에 대해 AFKAE0.5 모형의 모의 결과 투입부분으로 연간 강우, 관개, 수분상승량은 각각 1,043, 0, 207 mm이었으며 산출요인으로 증발산, 유거, 투수량은 각각 831, 309, 161 mm로 산출되어 투입된 물보다 산출된 물의 양이 88 mm 많았다. 또한 작부기간중 증발산량에 대해 지하에서 상승하는 수분량이 24.7%에 해당되어 건조기에 지하에서 모세관 등을 통해 상승하는 양도 상당한 것을 알 수 있었다. 작부기간 중 토양수분 측정값과 AFKAE0.5 모형의 모의 값과 토양수분함량의 변화를 일별로 비교한 결과 포화되었을 때의 수분함량이 유사하였으며 건조에 따른 수분함량 일별 감소비율이 매우 유사하게 나타나고 있어 모형이 적합한 것으로 평가되었다. 개발된 AFKAE0.5 토양 물수지 및 수분추정 모형은 농가에서 손쉽게 자가 포장에 대한 기본적인 영농이력을 알고 있으며 포장의 수분상태를 알 수 있고 적정관개를 할 수 있는 유용한 도구로서 역할을 할 것으로 기대된다. As the area of upland crops increase, it is become more important for farmers to understand status of soil water at their own fields due to key role of proper irrigation. In order to estimate daily water balance and soil water content with simple weather data and irrigation records, we have developed the model for estimating water balance and soil water content, called AFKAE0.5, and verified its simulated results comparing with daily change of soil water content observed by soil profile moisture sensors. AFKAE0.5 has two hypothesis before establishing its system. The first is the soil in the model has 300 mm in depth with soil texture. And the second is to simplify water movement between the subjected soil and beneath soil dividing 3 categories which is defined by soil water potential. AFKAE0.5 characterized with determining the amount of upward and downward water between the subjected soil and beneath soil. As a result of simulation of AFKAE0.5 at Gongju region with red pepper cultivation in 2005, the water balance with input minus output is recorded as ? 88 mm. the amount of input water as precipitation, irrigation, and upward water is annually 1,043, 0, and 207 mm, on the other, output as evapotranspiration, run-off, and percolation is 831, 309, and 161 mm, respectively.
Dong-Myung Lee,Jin-Woo Jung,Seo Weon Heo,Tae Heoung Kim 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.1
This paper proposes an analytic model for four-switch inverter (FSI)-driven wye (Y) or delta (Δ)-connected motors with a current ripple reduction algorithm. FSIs employ four switches in controlling three-phase load instead of using six switches. They have split dc-link stage, and due to this inherent structure there exists the voltage difference between upper and lower capacitors, which results in distortion of the inverter output voltage. To study characteristics of FSIs, this paper presents an advanced simulation models of FSI-driven control system for 3-phase motor that can has a wire connection either Y or Δ. In addition, this paper introduces a current ripple reduction scheme that mitigates degradation of control performance due to the voltage difference between the dc-link capacitors. The validity of the proposed method and the analytic model is verified by simulations and experiments carried out with 1-HP induction machine with Y or Δ-connection