Optimization of Ni-zirconia based anode support for robust and high-performance 5 x 5 cm² sized SOFC via tape-casting/co-firing technique and nano-structured anode

Myung, J.h.,Shin, T.h.,Kim, S.D.,Park, H.G.,Moon, J.,Hyun, S.H. Pergamon Press ; Elsevier Science Ltd 2015 International journal of hydrogen energy Vol.40 No.6

High-performance Ni-zirconia based anode supported cells were developed via a cost-effective tape-casting/co-firing technique and a nano structured anode. The fundamental properties for conventional NiO-YSZ anode supports - such as porosity, shrinkage, electrical conductivity and mechanical strength - were measured as a function of the proportion of NiO and YSZ (coarse and fine powders). Electrical conductivity, shrinkage, porosity, strength were found to be 1200 S/cm, 22%, 45% and 55 MPa, respectively, for a composition of NiO:YSZ (60:40 wt%) and coarse:fine YSZ (50:50 wt%). However, warping of the cell and delamination was frequently observed between the anode and the electrolyte after the co-firing step. The NiO/YSZ-ScSZ (40/30-30 wt%) nano-composite anode was synthesized to increase the connectivity of Ni phase, the sinter-ability of YSZ phases and to match the shrinkage with ScSZ electrolyte. It displayed strength of 95 MPa, an electrical conductivity of 1400 S/cm with thermal stability after cycling 10 times, 50% porosity, and 28% shrinkage; the latter being particularly similar to the ScSZ electrolyte. Moreover, the 5 x 5 cm<SUP>2</SUP> sized single cell consisting of the NiO/YSZ-ScSZ anode, ScSZ electrolyte and an LSM-YSZ cathode showed 19 @?/5 cm of flatness and a power of over 13.3 W (0.83 W/cm<SUP>2</SUP>) with hydrogen at 700 <SUP>o</SUP>C.

FRTL-5 세포의 세포주기(Cell Cycle)에 TSH 및 그레이브병 IgG가 미치는 영향에 관한 Flow Cytometry를 이용한 분석

박춘식,김극배,유명희,이희발 대한내분비학회 1992 Endocrinology and metabolism Vol.7 No.3

Controversy exists in the question that Graves' disease TSI (Thyroid stimulating immunoglobuin) and TGI (Thyroid growth immunoglobulin) coexist as different molecules or are the properties of a single IgG and current methods measuring TGI activity had been criticized, We tried to measure TGI activitty using FRTL-5 cells by flow ytometric analysis. The results were as follows. 1) Cell cycle analysis of FRTL-5 cells cultured without hormones (5% CS) showed 85.5±0.5% cells in Go and GI phase, 8.6±0.4% cells in S phase and 5.4±0.2% cells in G2M phase. When cultured with TSH(6 hormone medium), cells were 64.0±1.1% in Go and GI phase, 26.2±0.7% in S phase and 9.8±1.2% in G2M phase which means increased cell division. 2) When cultured with untreated Graves' IgG, the cell cycle analysis showed variable patterns. S phase percent in patients with goiter volume more than 40 ml was increasd (GII, 11.96±2.70 %) when compared with that of the patients with goiter volume less than 40 ml(GI, 9.90±2.57%). Overall, i8) of GI and 43%(7/16) of GII, Graves' IgG increased S phase percents when compared with normal IgG. These resluts suggested that flow cytometric analysis of the cell cycle might be a valuable method of TGI assay providing direct measurements of cell division.(J Kor Soc Endocrinol 7:228~233.1992)

Conserved sequences of thrombospondin‐related adhesive protein gene of <i>Plasmodium vivax</i> in clinical isolates from Korea

Nam, Myung H.,Jang, Jin W.,Kim, Hanna,Han, Eun T.,Lee, Won J.,An, Seong S. A.,Park, Ae S. D.,Lim, Chae S. Blackwell Publishing Ltd 2011 Tropical medicine & international health Vol.16 No.8

<P><B>Summary</B></P><P>Thrombospondin‐related adhesive protein (TRAP) from <I>Plasmodium vivax</I> (<I>P.?vivax</I>) became one of the important vaccine candidates for malaria, because <I>P.?vivax</I> TRAP (PvTRAP) is responsible for the sporozoite–host interactions. PvTRAP polymorphisms in the isolates from Republic of Korea (ROK) were analysed, setting the valuable baseline data for the future vaccine developments and clinical trials with PvTRAP, as a strong vaccine candidate. A total of 54 isolates were collected in 2010. PvTRAP genes from above isolates were amplified and sequenced, and the results were analysed and compared against Sal‐1 strain. Sequencing analysis of 1424‐bp‐size PvTRAP PCR products revealed one major allelic type with six non‐synonymous substitutions, where S81T, E95D, I121V and T127R substitutions were found in region II, and K371N and A425E substitutions from region IV. The ROK isolates revealed the limited sequence polymorphisms in PvTRAP in comparison with the reported isolates from other nations.</P>

자유드로잉 제품과 패드를 설치한 드로잉 제품의 특성

고관영,명노훈,하종성,이창수,최창권,최병용,허기영,김광수,김동수,장종훈,장윤철,김세환,오정민,하민정,박향자 울산과학대학 2000 연구논문집 Vol.27 No.1

본 연구는 자체 제작한 전체 패드가 부착된 드로잉 금형을 이용하여 SUS, 연강, AI, 6:4황동 판재를 드로잉 가공하여 생산된 제품의 특성을 자유드로잉 하여 얻은 제품의 특성과 비교 분석하였다. SUS 판재의 경우 제품에 발생하는 현상은 벽 주름, 구변주름, 수직 파단, 드로잉상처, 이어링이 발생하였으며 길이변화는 펀치어깨부분에서 가장 많이 늘어남을 볼 수 있었고 벽 주름 부분에 있어서 접혀진 부분은 상대적으로 적게 늘어남을 볼 수 있었다. 연강 판재는 SUS 판재에서 보여준 현상을 나타내었으나 SUS 판재에 비해 수직 파단은 적게 일어난 반면 이어링이 더 크게 일어났다. AI 판재의 경우 수직 파단은 일어나지 않았으나 플랜지 주름과 밑면터짐이 발생하였다. 6:4황동의 경우는 전체패드의 AI 판재 드로잉에서와 같이 밑면터짐과 플랜지 주름의 발생이 일어났다.

G₁ to S phase transition protein 1 induces apoptosis signal-regulating kinase 1 activation by dissociating 14-3-3 from ASK1

Lee, J A,Park, J E,Lee, D H,Park, S G,Myung, P K,Park, B C,Cho, S Nature Publishing Group 2008 Oncogene Vol.27 No.9

Apoptosis signal-regulating kinase 1 (ASK1), a member of the mitogen-activated protein kinase kinase kinase family, plays a critical role in mediating apoptosis signals initiated by a variety of death stimuli such as hydrogen peroxide and tumor necrosis factor-α. Owing to its critical role in inducing apoptosis, the activity of ASK1 is tightly regulated by various mechanisms such as post-translational modifications and protein–protein interactions. Here we describe the identification of G<SUB>1</SUB> to S phase transition protein 1 (GSPT1), which is associated with protein translation, as a regulator of ASK1. GSPT1 interacts with ASK1 and enhances ASK1-induced apoptotic activity through the activation of caspase-3. In vitro kinase assay data show that GSPT1 enhances ASK1 autophosphorylation and its kinase activity. Cell cycle-dependent GSPT1 induction and small interfering RNA analyses show that ASK1 autophosphorylation is dependent on the expression level of endogenous GSPT1 in cells. GSPT1 inhibits the binding of ASK1 to the 14-3-3 protein, an ASK1 inhibitor, while GSPT1 has no effect on the interaction between ASK1 and TRAF2, a C-terminal-binding activator of ASK1. Thus, our results reveal a novel role of GSPT1 in the regulation of ASK1-mediated apoptosis.Oncogene (2008) 27, 1297–1305; doi:10.1038/sj.onc.1210740; published online 20 August 2007

Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

Kim, Hak-Gon,Song, Hyun-Jin,Jeong, Mi-Jin,Sim, Seon-Jeong,Park, Dong-Jin,Yang, Jae-Kyung,Yoo, Seok-Bong,Yeo, Jin-Ki,Karigar, Chandrakant S.,Choi, Myung-Suk Institute of Forest Science 2011 Journal of Forest Science Vol.27 No.3

The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

The effects of topical mesenchymal stem cell transplantation in canine experimental cutaneous wounds

Kim, Ju-Won,Lee, Jong-Hwan,Lyoo, Young S,Jung, Dong-In,Park, Hee-Myung Blackwell Publishing Ltd 2013 Veterinary dermatology Vol.24 No.2

<P><B>Background</B></P><P>Adult stem cells have been widely investigated in bioengineering approaches for tissue repair therapy. We evaluated the clinical value and safety of the application of cultured bone marrow-derived allogenic mesenchymal stem cells (MSCs) for treating skin wounds in a canine model.</P><P><B>Hypothesis</B></P><P>Topical allogenic MSC transplantation can accelerate the closure of experimental full-thickness cutaneous wounds and attenuate local inflammation.</P><P><B>Animals</B></P><P>Adult healthy beagle dogs (<I>n</I> = 10; 3–6 years old; 7.2–13.1 kg) were studied.</P><P><B>Methods</B></P><P>Full-thickness skin wounds were created on the dorsum of healthy beagles, and allogenic MSCs were injected intradermally. The rate of wound closure and the degree of collagen production were analysed histologically using haematoxylin and eosin staining and trichrome staining. The degree of cellular proliferation and angiogenesis was evaluated by immunocytochemistry using proliferating cell nuclear antigen-, vimentin- and α-smooth muscle actin-specific antibodies. Local mRNA expression levels of interleukin-2, interferon-γ, basic fibroblast growth factor and matrix metalloproteinase-2 were evaluated by RT-PCR.</P><P><B>Results</B></P><P>Compared with the vehicle-treated wounds, MSC-treated wounds showed more rapid wound closure and increased collagen synthesis, cellular proliferation and angiogenesis. Moreover, MSC-treated wounds showed decreased expression of pro-inflammatory cytokines (interleukin-2 and interferon-γ) and wound healing-related factors (basic fibroblast growth factor and matrix metalloproteinase-2).</P><P><B>Conclusion and clinical importance</B></P><P>Topical transplantation of MSCs results in paracrine effects on cellular proliferation and angiogenesis, as well as modulation of local mRNA expression of several factors related to cutaneous wound healing.</P><P><B>Résumé</B></P><P><B>Contexte</B></P><P>Les cellules souches adultes ont été largement étudiées dans les approches de bio-ingénierie pour la thérapie de réparation tissulaire. Nous évaluons l'efficacité clinique et la sécurité de l'application de cellules souches mésenchymateuses allogéniques en culture dérivées de moelle osseuse (MSCs) pour le traitement de plaies cutanées dans un modèle canin.</P><P><B>Hypothèse</B></P><P>La transplantation de MSC allogénique topique peut accélérer la fermeture en toute épaisseur de plaies cutanées expérimentales et atténuer l'inflammation locale.</P><P><B>Sujets</B></P><P>Des chiens beagles adultes sains (<I>n</I> = 10; 3–6 ans; 7.2–13.1 kg) ont été étudiés.</P><P><B>Méthodes</B></P><P>Des plaies cutanées en pleine épaisseur ont été crées sur la face dorsale des beagles sains et des MSCs allogènes ont été injectées par voie intradermique. Le taux de cicatrisation et le degré de production de collagène ont été analysés sur le plan histologique par colorations à l'hématoxyline et éosine et par trichrome. Le degré de prolifération cellulaire et d'angiogénèse ont été évalués par immunohistochimie à l'aide d'anticorps spécifiques d'antigène nucléaire de prolifération cellulaire, de vimentine et d'actine de muscle lisse α. Les taux d'expression local d'ARNm d'interleukine-2, d'interféron-γ, du facteur de croissance basique de fibroblaste et de métalloprotéinase-2 de matrice, ont été évalués par RT-PCR.</P><P><B>Résultats</B></P><P>Comparé avec les plaies traité

Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

Hak-Gon Kim,Hyun-Jin Song,Mi-Jin Jeong,Seon-Jeong Sim,Dong-Jin Park,Jae-Kyung Yang,Seok-Bong Yoo,Jin-Ki Yeo,Chandrakant S. Karigar,Myung-Suk Choi 강원대학교 산림과학연구소 2011 Journal of Forest Science Vol.27 No.3

The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

가연성 가스 검출용 Figaro형 Pd/Al₂O₃/SnO₂계 센서 제조와 그 감응특성

명광식,한상도,이상호,박기배,손영목 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

Figaro type gas sensor was fabricated with sensing materials in which mixed with SnO_(2) , Al_(2)O_(3) binder and palladium catalyst and, developed electronic parts. The SnO_(2) powders obtained by sol-gel method had a mean particle size of 0.93 p m and specific surface area of 32.5 m^(2)/g. And the γ-Al_(2)O_(3)with surface area of 180 m^(2)/g and a solution of 1M. PdCl_(2) were used for the synthesis of the sensing material. Mullite tubes of 0.8mm diamrter, Au pad electrodes of 0.3mm clearance, Au-Pd alloy lead wire of 80μm dia, heating element coiled with Kanthal wire of 60 μm dia were developed to use the gas sensor. The sensitivity of the sensors was excellent for the detection of methane, propane and carbon monoxide at about 490℃, 440℃ and 140℃, respectively.

N,N'-비스[(2,2-디메틸-1,3-디옥솔-4-일)메틸]우레아의 간편한 합성

박명숙,Gibson,Frank S.,Rapoport, Henry 德成女子大學校 藥學硏究所 1994 藥學論文誌 Vol.5-6 No.1

Carbodinmide는 유기 합성에 유용한 시약이다. 상업적으로 이용할 수 있는 dicyclohexylcarbodiimide(DCC)는 peptide synthesis에 널리 사용되어 왔다. Diisopropylcarbodiimide (DIC)나 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride(EDC)와 같은 carbodiimide는 peptide coupling reagent로 쓰이고 있다.