Effects of pyridoxine on a high‐fat diet‐induced reduction of cell proliferation and neuroblast differentiation depend on cyclic adenosine monophosphate response element binding protein in the mouse dentate gyrus

Yoo, Dae Young,Kim, Woosuk,Yoo, Ki‐,Yeon,Nam, Sung Min,Chung, Jin Young,Yoon, Yeo Sung,Won, Moo‐,Ho,Hwang, In Koo Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.90 No.8

<P><B>Abstract</B></P><P>In this study, we challenged pyridoxine to mice fed a high‐fat diet (HFD) and investigated the effects of pyridoxine on HFD‐induced phenotypes such as blood glucose, reduction of cell proliferation and neuroblast differentiation in the dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a commercially available low‐fat diet (LFD) as control diet or HFD (60% fat) for 8 weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was administered for 3 weeks. The administration of pyridoxine significantly decreased body weight in the HFD‐treated group. In addition, there were no significant differences in hepatic histology and pancreatic insulin‐immunoreactive (‐ir) and glucagon‐ir cells of the HFD‐treated group after pyridoxine treatment. In the HFD‐fed group, Ki67‐positive nuclei and DCX‐ir neuroblasts were significantly decreased in the dentate gyrus compared with those in the LFD‐fed mice. However, the administration of pyridoxine significantly increased Ki67‐positive nuclei and DCX‐ir neuroblasts in the dentate gyrus in both LFD‐ and HFD‐fed mice. In addition, the administration of pyridoxine significantly increased the protein levels of glutamic acid decarboxylase 67 (GAD67) and brain‐derived neurotrophic factor (BDNF) and the immunoreactivity of phosphorylated cyclic AMP response element binding protein (pCREB) compared with the vehicle‐treated LFD‐ and HFD‐fed mice. In contrast, the administration of pyridoxine significantly decreased HFD‐induced malondialdehyde (MDA) levels in the hippocampus. These results showed that pyridoxine supplement reduced the HFD‐induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA. © 2012 Wiley Periodicals, Inc.</P>

농업용 저수지 퇴적물 준설과 처리 시범사업의 경제성 평가

유진채,여순식,공기서 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.2

The objectives of this study were evaluating economic efficiency of pilot projects for dredging and treatment in agricultural reservoirs using methods of costs benefits analysis. The pilot projects areas included four locals: Giheung, Seolseong, Dogo and Habin. The projects period assumed 20 years. The net present value of whole pilot projects was estimated 9,143 million Won. The costs benefits ratio was estimated 3.08. The internal rate of returns was estimated 53.4%. The results of evaluating economic efficiency of pilot projects were feasible based on costs benefits analysis

SRM 구동용 인버터 토폴로지의 특성비교

여진기,성세진,이흥호 충남대학교 공업교육연구소 1998 論文集 Vol.21 No.1

This paper presents a drive circuit and comparision of property of inverter topology for SRM with improved torque, as rapidity reducing current within maximum inductance region. Not considered in this paper is another class of inverter topologies for motor with bifilar windings. The proposed converter is applied to 3phase, 6/4 SRM.

분심기음의 항암작용 및 면역기능에 관한 연구

여대원,김진성,윤상협,류봉하,류기원 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

This study was performed to investigate the effect of Bunsimgieum on antitumor effect after sarcoma-180 cells transplantation into peritoneal cavity or left groin and immune responses on the depressed immunity induced by methotrexate in mice. The Bunsimgieum extract of 10㎎/㎏ was orally administered 14 days for antitumor effects and 21 days for immune responses. 50% inhibitory concentration(IC_(50)) of SUN-1, SUN-C4. and SUN-396 cancer cell, mean sunvival days and body weight of tumor bearing mice, and growth of tumor mass for antitumor effect; delayed type hypersentivity, hemagglutinin titer, hemolysis titer, rosette forming cells, natured killer cell activity, lymphocyte transformation. productivity of interleukin-2 and phagocytic activity for their immune responses were measured in ICR mice. Significance in antitumor effect is noted in the enlongation of mean life days and inhibition of tumor growth(p<0.01 respectively). Significance of immune responses is also noted in hemolysis titer, lymphocyte transfumotion IL-2 productivity, phagocytic activity, and natural killer cell activity at E/T ratio 100 : 1(p<0.01, respectively). Significant in rosette cell formation was seen dosage of 20㎎/㎏(p<0.01) However, Difference of body weight as antitumor effect, delayed type hypersensitivity, and hemagglutinin titer were not shown significantly. According to the above results, it could be suggested that Bunsimgieum has prominent antitumor and immunity enhancing effect.

국제해운항만로지스틱스에 있어서 항만경쟁력의 평가에 관한 연구 : 계층퍼지분석법의 적용 An Application of Hierarchical Fuzzy Process

김진구,여기태,이종인 한국로지스틱스학회 2002 로지스틱스연구 Vol.10 No.2

본 연구의 목적은 국제해운항만로지스틱스에 있어서 근간이 되는 경쟁항만의 현황을 조사하고, 실제 경쟁을 주도하는 구성요소를 파악하며, 항만경쟁력 평가모델을 개발하고, 평가모델에 각 항만의 구성요소별 data를 투입하여 실제 경쟁능력을 파악하는 것이다. 연구의 방법론은 속성간 중복도를 고려하여 연산 할 수 있는 계층퍼지분석법(HFP : Hierarachical Fuzzy Process)을 도입하였다. 그리고, 연구의 범위는 급변하는 국제해운항만로지스틱스에 있어서 가장 관심의 대상이 되고 있는 동북아시아국가 및 동남아시아국가의 컨테이너항문 중 2000년 컨테이너 처리실적 상위 20위 내에 위치한 항만을 대상으로 하였다. 연구의 결과 싱가포르 항만이 연구대상지역에서는 가장 경쟁력이 뛰어난 것으로 나타났다. 향후 연구과제로, 본 연구의 항만경쟁력 평가기법으로 채택된 HFP방법에 있어서 엄밀히 고려하지 못한 다수의사결정그룹(Multiple Decision-Making Group : MDMG)의 해결을 위한 보완적 연구가 더 필요하다. The purpose of this study is to grasp the real competitiveness of ports in international shipping and port logistics, by 1) examining the status of competitive ports, 2) grasping the components that lead the actual competition, 3) developing evaluation models of port competitiveness, and 4) putting component data of each port into these same evalation models. The research method which was used in this study accounted for overlapping between attributes, and introduced the HEP(Hierarchical Fuzzy Process) method that can perform an operation. The scope of this study covers the top 20 Northeast and Southeast Asian ports with respect to container handling in 2000. This has direct competition in attracting cargo from East Asia. The results of this study showed that the competitive power of Singapore Port was the highest among the ports of East Asia. Derived from the empirical study, the attempt to grasp the real port competitiveness in dynamic port activities is the merit of this study, especially in the rapidly changing environments of international shipping and port logistics. In order to reinforce this study, it needs further compensative research because the HFP method adopted as an evaluation model of port competitiveness in this study was not strictly considered enough to solve the problems such as MDMG(Multiple Decision-Making Group).

트리암시놀론 아세토니드를 함유한 구강점막 부착성 패치의 제조 및 평가

엥흐자야,여동기,박진석,이은주,이경록,박정숙 충남대학교 약학대학 의약품개발연구소 2013 藥學論文集 Vol.28 No.-

The objective of this study was to prepare triamcinolone acetonide-loaded buccal adhesive patch. The adhesive patch was formulated by casting method using aqueous soluble polymer povidone K17 (PVP 17PF) as a film-forming agent and hydroxypropylmethylcellulose (HPMC) as an adhesive agent. To compare the effect of HPMC type, different molecular weight of K4M and K15M HPMC was used. The physicochemical properties of patches such as appearance, thickness, in vitro release, and adhesiveness were investigated. The concentration of triamcinolone acetonide was determined spectrophotometrically at a wavelength of 240 nm. The appearance of prepared patches was semi-transparent, light-yellow or almost colorless, and odorless. Thickness of each patches (n=6) was 0.942 ± 0.026 mm for K4M patch and 0.703 ± 0.036 mm for K15M patch, respectively. In vitro release test, both K4M and K15M patches showed over 20% release within 30 min. At 120 min, K4M and K15M patches demonstrated 80% and 76% release of triamcinolone acetonide, respectively, and up to 240 min, both patches released drug completely. Maximum adhesive force of K4M and K15M patches was 4.66 ± 0.76 gf and 2.69 ± 0.90 gf, respectively. Moreover, it took 28.73 ± 0.44 sec and 28.68 ± 0.61 sec for K4M and K15M patch to peel off them after adhesion, showing no significant difference. In conclusion, thickness, in vitro release, and maximum adhesive force could be modulated by alteration of polymer types.

In situ Gel Forming Stereocomplex Composed of Four-Arm PEG-PDLA and PEG-PLLA Block Copolymer

Yeo Jin Jun,Kyung Min Park,Yoon Ki Joung,Ki Dong Park,Seung Jin Lee 한국고분자학회 2008 Macromolecular Research Vol.16 No.8

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesdthe major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-a, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.1

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesd-the major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.

대도시 교통혼잡도 산정에 관한 연구

여혁진(Yeo Hyuk Jin),정진혁(Chung Jin Hyuk),김익기(Kim Ik Ki) 대한토목학회 2005 대한토목학회논문집 D Vol.25 No.5D