http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Steady and Dynamic Shear Rheology of Fucoidan-Buckwheat Starch Mixtures
Cho, MyoungLae,Choi, Won-Seok,You, SangGuan WILEY-VCH Verlag 2009 Die Stärke Vol.61 No.5
<P>Rheological properties of fucoidan (F) and buckwheat starch (B) mixtures (3% or 6%) at different blending ratios of fucoidans (0, 0.1, 0.2, 0.5 and 1.0%) were investigated in steady and dynamic shear. Steady shear viscosity measurement revealed that aqueous pastes of the BF blends (3%, w/v) had a pseudoplastic and shear-thinning behavior with flow behavior index (n) values of 0.61–0.68. The substitution of starch with fucoidan polymers significantly lowered the apparent viscosities compared with the pure starch paste and, when mixed with less than 0.5% of fucoidan, the viscosities of the pastes were even lower than those of the starch pastes at the corresponding starch concentrations. According to dynamic viscoelastic measurement performed at 6% total carbohydrate concentration, buckwheat starch mixtures behaved like weak gels and the BF blends containing less than 0.5% fucoidan had considerably lower storage (G') and loss (G') moduli than the starch paste at the corresponding starch concentrations. However, the magnitude of G' increased with fucoidan concentrations over 0.5%, suggesting that a concentration of fucoidans > 0.5% might enhance the formations of three-dimensional networks and crosslinking of the starch samples, probably because of the mutual exclusion between starch and fucoidan polymers through the phase separation process. This study indicates that it is possible to obtain the BF blends having various rheological properties by changing the concentration of fucoidan polymers.</P>
Immunomodulatory activity of sulfated polysaccharides from Agarum cribrosum
MyoungLae Cho,Dong-Jin Lee,SangGuan You 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Sulfated polysaccharides extracted from Agarum cribrosum and its fractionated using ion-exchange chromatography (crude, F1 and F2) were investigated to determine their molecular characteristics and biological activities. The crude and fractionated polysaccharides (F1 and F2) contained mostly of carbohydrates (52.4-56.0%), sulfates (12.7-23.0%) and uronic acid (14.1-21.8%) with small amount of proteins (3.9-9.3%). The monosaccharides levels of sulfated polysaccharides were mainly included fucose (43.1-44.2%), mannose (17.9-29.2%) and galactose (5.6-33.0%) with minor amount of rhamnose (2.3-4.6%) and glucose (0.9-3.5%). The sulfated polysaccharides contained one or two subfractions with average molecular weights (Mw) ranging from 123 × 103 to 765 × 103 g/mol. The sulfated polysaccharides, especially the F1 and F2 fractions, strongly stimulated mouse macrophage cells, Raw 264.7 cells, producing considerable amount of nitric oxide (NO), tumor necrosis factor-α (TNF-a) and various cytokines (IL 1-β, COX-2 and iNOS) which suggested that they could be strong immunostimulators. The overall results implies that the strong immunomodulatory activity of sulfated polysaccharides from A. cribrosum might be good sources of natural healthcare products, such as supplements and nutraceuticals.
조명래,이종석,이사라,손연경,배창환,여주홍,이해석,마진경,이옥환,김종예,Cho, MyoungLae,Lee, Jong Seok,Lee, Sarah,Son, Youn Kyoung,Bae, Chang-Hwan,Yeo, Joohong,Lee, Hae-Sock,Ma, Jin-Gyeong,Lee, Ok-Hwan,Kim, Jong-Yae 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.6
This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.
Ham, Sangwoo,Kim, Hyojung,Hwang, Seojin,Kang, Hyunook,Yun, Seung Pil,Kim, Sangjune,Kim, Donghoon,Kwon, Hyun Sook,Lee, Yun-Song,Cho, MyoungLae,Shin, Heung-Mook,Choi, Heejung,Chung, Ka Young,Ko, Han Seo Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.6
Aggregates of disease-causing proteins dysregulate cellular functions, thereby causing neuronal cell loss in diverse neurodegenerative diseases. Although many in vitro or in vivo studies of protein aggregate inhibitors have been performed, a therapeutic strategy to control aggregate toxicity has not been earnestly pursued, partly due to the limitations of available aggregate models. In this study, we established a tetracycline (Tet)-inducible nuclear aggregate (${\beta}23$) expression model to screen potential lead compounds inhibiting ${\beta}23$-induced toxicity. High-throughput screening identified several natural compounds as nuclear ${\beta}23$ inhibitors, including peucedanocoumarin III (PCIII). Interestingly, PCIII accelerates disaggregation and proteasomal clearance of both nuclear and cytosolic ${\beta}23$ aggregates and protects SH-SY5Y cells from toxicity induced by ${\beta}23$ expression. Of translational relevance, PCIII disassembled fibrils and enhanced clearance of cytosolic and nuclear protein aggregates in cellular models of huntingtin and ${\alpha}$-synuclein aggregation. Moreover, cellular toxicity was diminished with PCIII treatment for polyglutamine (PolyQ)-huntingtin expression and ${\alpha}$-synuclein expression in conjunction with 6-hydroxydopamine (6-OHDA) treatment. Importantly, PCIII not only inhibited ${\alpha}$-synuclein aggregation but also disaggregated preformed ${\alpha}$-synuclein fibrils in vitro. Taken together, our results suggest that a Tet-Off ${\beta}23$ cell model could serve as a robust platform for screening effective lead compounds inhibiting nuclear or cytosolic protein aggregates. Brain-permeable PCIII or its derivatives could be beneficial for eliminating established protein aggregates.