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Proteomic analysis of mammalian basic proteins by liquid-based two-dimensional column chromatography
Shin, Yu-Kyong,Lee, Hyoung-Joo,Lee, Joon Seok,Paik, Young-Ki WILEY-VCH 2006 Proteomics Vol. No.
<P>To develop a standard method for separating highly basic proteins in mammalian cells, we established a 2-D LC separation system coupled with chromatofocusing/nonporous RP column chromatography (CF/NPRPC) in a ProteomeLab PF2D system. After standardizing conditions for 2-D LC, a 2-D liquid protein map of uninfected macrophage proteins with pH range 8.3–11.3 was constructed, and then compared with a macrophage protein map made after infection with Candida albicans. The results demonstrate that 2-D LC offers both high resolution and reproducibility for separation of highly basic, macrophage proteins. After protein identification using a nano 2-D LC-MS/MS Proteomics Solution System, quantitative determination of the changes in the differentially expressed proteins (e.g., galectin-3) in C. albicans-infected macrophages was also accomplished by measuring the peak area of the chromatogram in 2-D LC. The result from this measurement of galectin-3 expression shows a 3.41-fold decrease in the infected macrophage cells, which was further confirmed by that from the RT-PCR of mRNA of galectin-3. Thus, 2-D LC coupled with CF/NPRPC could be applicable to common analysis of highly basic proteins in a high-throughput manner.</P>
Alterations of protein expression in macrophages in response to Candida albicans infection.
Shin, Yu-Kyong,Kim, Ki-Young,Paik, Young-Ki Korean Society for Molecular Biology 2005 Molecules and cells Vol.20 No.2
<P>Although macrophages are an important first line of cellular defense, they are unable to effectively kill phagocytosed C. albicans. To determine the physiological basis of this inability, we investigated the alterations of macrophage proteins caused by C. albicans infection. Since the formation of C. albicans hyphae caused cell death, proteins were prepared 3 h after infection and examined by two-dimensional gel electrophoresis (2-DE). The most prominent changes were in glycolytic enzymes, which could have caused energy depletion of the infected cells. Also changed were proteins involved in maintenance of cellular integrity and NO production. Treatment of the macrophages with either cytochalasin D or taxol did not alter their inability to kill C. albicans. Our results indicate that multiple factors contribute to cell death as the pathogenic form of C. albicans becomes fully active inside macrophage cells.</P>
Kim, Yu Kyong,Lee, Juyoung,Oh, Jaeseong,Rhee, Su-jin,Shin, Seung Han,Yoon, Seo Hyun,Lee, SeungHwan,Kim, Han-Suk,Yu, Kyung-Sang American Society for Microbiology 2019 Antimicrobial Agents and Chemotherapy Vol.63 No.6
<P>Fluconazole is an antifungal agent with reported evidence for its prophylactic effect against systemic fungal infection in preterm infants. The aim of this study was to build a population pharmacokinetic model to evaluate the pharmacokinetic characteristics of intravenous and oral fluconazole in preterm infants with the current prophylactic fluconazole dosing regimen.</P><P>Fluconazole is an antifungal agent with reported evidence for its prophylactic effect against systemic fungal infection in preterm infants. The aim of this study was to build a population pharmacokinetic model to evaluate the pharmacokinetic characteristics of intravenous and oral fluconazole in preterm infants with the current prophylactic fluconazole dosing regimen. A pharmacokinetic model was developed using 301 fluconazole concentrations from 75 preterm infants with a baseline body weight (WT) ranging from 0.5 to 1.5 kg and an estimated glomerular filtration rate (eGFR) ranging from 12.9 to 58.5 ml/min/1.73 m<SUP>2</SUP>. Eligible infants received an intravenous or oral dose of 3 mg/kg of body weight of fluconazole, twice weekly with a ≥72-h dose interval, for 4 weeks. The model was qualified with basic goodness-of-fit diagnostics, visual predictive checks, and bootstrapping. The fluconazole pharmacokinetics was well described with a one-compartment linear model with a proportional residual error. The population clearance (CL) and volume of distribution (<I>V</I>) were derived as 0.0197 × (WT/1.00)<SUP>0.746</SUP> × (eGFR/25.0)<SUP>0.463</SUP> × exp(η) and 1.04 × WT × exp(η), respectively. Such covariate analyses augment the awareness of the need for personalized dosing in preterm infants. (This study has been registered at ClinicalTrials.gov under identifier NCT01683760.)</P>
Pharmacokinetics and tolerability of eletriptan hydrobromide in healthy Korean subjects
Kim, Yu Kyong,Shin, Kwang-Hee,Alderman, Jeffrey,Yu, Kyung-Sang,Jang, In-Jin,Lee, SeungHwan Dove Medical Press 2018 Drug design, development and therapy Vol.12 No.-
<P><B>Background</B></P><P>Migraine is one of the most common headache disorders that greatly affect the quality of life. Selective serotonin (5-HT) receptor agonists such as triptamine-based drugs called triptans are used for treatment of migraine.</P><P><B>Purpose</B></P><P>This study aimed to evaluate the pharmacokinetic (PK) and tolerability profiles of eletriptan hydrobromide (eletriptan HBr), a selective 5-hydroxytryptamine (also known as serotonin) 1B/1D receptor agonist, in Koreans and compare the results to those observed in non-Koreans in a previously published study.</P><P><B>Patients and methods</B></P><P>A randomized, open-label, single, and repeated-dose study was conducted in 16 healthy Korean male subjects using a four-treatment, four-period, and four-sequence crossover design (NCT01139515). The subjects received one of the following four treatments in each period: a single dose of 20, 40, 80 mg eletriptan HBr or a repeated oral dose of 40 mg 2 h apart. Blood samples were collected before and up to 26 h after dosing for quantification of plasma eletriptan concentration by high-performance liquid chromatography tandem–mass spectrometry. The PK parameters were estimated using noncompartmental methods. Ethnicity differences between Korean and non-Korean subjects were identified using geometric mean ratios and 90% confidence intervals (CIs) of dose-normalized maximum plasma concentration (C<SUB>max</SUB>) and dose-normalized area under the plasma concentration versus time curve from 0 h to the last measurable concentration (AUC<SUB>0–t</SUB>).</P><P><B>Results</B></P><P>After single-dose administration of eletriptan HBr to Korean subjects, the mean C<SUB>max</SUB> and AUC<SUB>0–t</SUB> increased linearly with dose. Comparable total systemic exposures were observed in the 2 h apart 40 mg repeated and single 80 mg dose. The geometric mean ratios (90% CIs) of the dose-normalized C<SUB>max</SUB> and AUC<SUB>0–t</SUB> of Korean subjects were similar to those of non-Korean subjects reported in the literature. The adverse events observed were transient and mild in severity.</P><P><B>Conclusion</B></P><P>Eletriptan HBr showed linear PK and was well tolerated in Korean subjects. The PK and tolerability of eletriptan HBr did not differ between Korean and non-Korean subjects.</P>
Kyong-Jin Min,A-Min Kwak,E-Eun Jeong,Hae-lin Yu,Mi-Rae Shin,Hee-Wan Kang 한국버섯학회 2017 버섯 Vol.21 No.1
This study aimed to investigate antioxidant activity of various extracts from fruiting bodies and mycelia of two Phellinus linteus strains and P. baumii. The Phellinus strains have cultivated on oak and mulberry logs. The fruiting bodies species were harvested from each Phellinus strain and used in this study. The tested items include: 2, 2’-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS), free radical scavenging assay and determination of total phenolics contents (TPC), ferric reducing antioxidant power assay (FRAP), and DPPH (1, 1-diphenyl-2- picrylhydrazyl) radical-scavenging activities. Different extractions with 60% Ethyl alcohol, 70% methyl alcohol and heat water were done on the mycellial and fruiting bodies samples of the mushroom species. The methyl alcohol extraction from fruiting body of P. linteus KACC93057P displayed the highest antioxidant activity on ABTS, FRAP, and DPPH assays. The ethyl acetate fraction was concentrated and subjected to an ODS column chromatography, followed by Sephadex LH-20 column chromatography. Finally six compounds 1-6 were detected by preparative reversed-phase HPLC.
Obstetric factors associated with non-cephalic presentation in late pregnancy
( Yu Mi Shin ),( Eun Ji Oh ),( Kyong-no Lee ),( Hyeon Ji Kim ),( Jee Yoon Park ),( Kyung Joon Oh ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-
Objective: To determine the factors affecting fetal presentation through prediction model Methods: A retrospective cohort study was performed in pregnant women who had visited Seoul National University Bundang Hospital for prenatal check-ups from October 2018 to November 2021. Triplet pregnancies (n=27) and the cases without ultrasonography results (n=5) were excluded. The ultrasonography reports with fetal presentation throughout the pregnancy were collected and anayzed. Various obstetric information was reviewed through electronic medical records. Results: There were 2,543 cases of fetuses with ultrasonography after 32 weeks of gestation and the proportions of cephalic, breech, and transverse presentation were 84.7% (2,154), 13.1% (332), and 2.2% (57), respectively. In the analysis to compare the obstetric factors between those with cephalic presentation and with non-cephalic presentation in late pregnancy, maternal age, pre-pregnancy body mass index, the rates of nulliparity, twin pregnancy, hypertensive disorder in pregnancy, history of previous non-cephalic presentation and small-for-gestational age (SGA) were significantly higher in non-cephalic group than cephalic group (all p-value < 0.05). However, the rates of previous vaginal delivery and alleged myoma uteri were significantly lower in non-cephalic group (both p-value < 0.05). In multivariate analysis, twin pregnancy was associated with higher rate of non-cephalic presentation with odds ratio (OR) 2.8, 95% confidence interval (CI) 2.0-4.0, and p-value <0.001. The non-presenting twin (Twin B) (OR 1.5, 95% CI 1.1-2.1, p=0.012) and previous non-cephalic presentation (OR 4.5, 95% CI 1.7-11.6, p=0.002) were significantly associated with increased rate of non-cephalic presentation as well. Conclusion: Twin pregnancy and previous history of non-cephalic presentation baby seemed to increase the possibility of non-cephalic presentation in late pregnancy. In twin pregnancy, especially the second twin was the independent risk factor for non-cephalic presentation