Rapid preparation and characterization of chitosan nanoparticles for oligonucleotide

Mu-hua Cheng,Yao-xiong Huang,Han-jian Zhou,Zhi Liu,Jian-fang Li 한국물리학회 2010 Current Applied Physics Vol.10 No.3

Chitosan is regarded as one of the potential candidates as a gene carrier. However, the poor solubility of chitosan is the major limiting factor in its utilization as a gene carrier. The purpose of this study was to simplify the method of preparing the nanoparticles of chitosan linked with antisense oligonucleotide (asON). The main step was preparing the derivatives of chitosan phosphate (CSP) in order to easily dissolve in aqueous solution. The nanoparticles were formed using a simple mixed method for CSP and asON, and the nanoparticle’s forming condition was optimized so that the nanoparticle’s characterization could be examined. Results showed that it was simple to make the nanoparticles under the optimal condition of 2:1 M proportion of CSP and asON. The size of the nanoparticles was 102.6 ± 12.0 nm, its zeta potential was 1.45 ± 1.75, and the encapsulated ratio of the chitosan crosslinked the asON was 87.6 ± 3.5%. The infrared spectra and electron microscope displayed that chitosan may combine with the asON to form equirotal nanoparticles. In conclusion, it was simple and feasible to form chitosan nanoparticles for asON using the CSP, and the CSP can efficiently encapsulate asON.

Clinical Outcomes of Basaloid Squamous Cell Carcinoma of the Esophagus: A Retrospective Analysis of 142 Cases

Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.

Review on Silkworm (Bombyx mori)Sex Control in China

An-Ying Xu,Mu-Wang Li,Ping-Jiang Sun,Yue-Hua Zhang,Cheng-Xiang Hou 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2

Normally, silkworms, Bombyx mori, generate offspring by sexual activity. As we known, the hybrids of the first generation of the silkworm have higher cocoon production than pure lines. During the sericulture production, many processes are related with sex control. For example, sex sorting in the egg grainages, rearing of only male silkworm to save the mulberry leaf consumption and increase silk output and quality. Therefore it is very interested in understanding the sex control of the silkworm in theory and practice. Chinese sericultural scientists have been being engaged in the researches in the fields of artificifial parthogenesis, dispermic androgenesis, sex-limited varieties, sex linkage balanced lethal strain and high temperature sensitive male stocks for several decades and gained substantial achievement. Some of the achievements have been used in the commercial production. In this review, the authors introduced that the methods for control of the silkworm sex, and regulate the silkworm sex ratio according to different producing aim in the world and especially in China.

Review : Review on Silkworm (Bombyx mori) Sex Control in China

( An Ying Xu ),( Mu Wang Li ),( Ping Jiang Sun ),( Yue Hua Zhang ),( Cheng Xiang Hou ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2

Normally, silkworms, Bombyx mori, generate offspring by sexual activity. As we known, the hybrids of the first generation of the silkworm have higher cocoon production than pure lines. During the sericulture production, many processes are related with sex control. For example, sex sorting in the egg grainages, rearing of only male silkworm to save the mulberry leaf consumption and increase silk output and quality. Therefore it is very interested in understanding the sex control of the silkworm in theory and practice. Chinese sericultural scientists have been being engaged in the researches in the fields of artificifial parthogenesis, dispermic androgenesis, sex-limited varieties, sex linkage balanced lethal strain and high temperature sensitive male stocks for several decades and gained substantial achievement. Some of the achievements have been used in the commercial production. In this review, the authors introduced that the methods for control of the silkworm sex, and regulate the silkworm sex ratio according to different producing aim in the world and especially in China.

Review on Silkworm (Bombyx mori) Sex Control in China

Xu, An-Ying,Li, Mu-Wang,Sun, Ping-Jiang,Zhang, Yue-Hua,Hou, Cheng-Xiang Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.2

Normally, silkworms, Bombyx mori, generate offspring by sexual activity. As we known, the hybrids of the first generation of the silkworm have higher cocoon production than pure lines. During the sericulture production, many processes are related with sex control. For example, sex sorting in the egg grainages, rearing of only male silkworm to save the mulberry leaf consumption and increase silk output and quality. Therefore it is very interested in understanding the sex control of the silkworm in theory and practice. Chinese sericultural scientists have been being engaged in the researches in the fields of artificifial parthogenesis, dispermic androgenesis, sex-limited varieties, sex linkage balanced lethal strain and high temperature sensitive male stocks for several decades and gained substantial achievement. Some of the achievements have been used in the commercial production. In this review, the authors introduced that the methods for control of the silkworm sex, and regulate the silkworm sex ratio according to different producing aim in the world and especially in China.

The Effect of Level Statistics and Fluctuations on the Specific Heat of Small Metallic Superconducting Grains

Yong Yao,Li-Hua Ma,Yu-Xiao Liu,Deng-Mu Cheng,Yi Qiu,Li-Sha Wu,Yun Qi 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.5

The thermodynamic properties of small metallic superconducting grains are affected by fluctuations in the order parameter and the level statistics, especially in the critical region. Here, the static path approximation was applied to deal with the fluctuations and the thermodynamic contribution of quasiparticles, and random matrix theory was applied to deal with level statistics. The specific heats were calculated numerically in the cases of even and odd number of electrons and different spins.

Research Articles : A Biovoltine Silkworm Variety, Huayuan × Dongshen, That is Resistant to Fluoride Contamination

( An Ying Xu ),( Chang Qi Lin ),( Cheng Xiang Hou ),( Yue Hua Zhang ),( Mu Wang Li ),( Ping Jiang Sun ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.13 No.1

Implementation of the Land Surface Processes into a Vector Vorticity Equation Model (VVM) to Study its Impact on Afternoon Thunderstorms over Complex Topography in Taiwan

Chien-Ming Wu,Hsiao-Chun Lin,Fang-Yi Cheng,Mu-Hua Chien 한국기상학회 2019 Asia-Pacific Journal of Atmospheric Sciences Vol.55 No.4

In this study, we aim to evaluate the impact of fast land-atmosphere interactions on the afternoon thunderstorm in Taiwan through high-resolution meteorological simulations. For this purpose, the Noah land surface model (LSM) is implemented into the vector vorticity equation cloud-resolving model (VVM) with corresponding realistic land surface data of Taiwan into the coupling system, called TaiwanVVM. Two idealized experiments are conducted by giving the same surface forcing but one with direct land-atmosphere coupling from Noah LSM (called Coupled experiment) and the other with prescribed surface fluxes (called Prescribed experiment). Our results show that the fast land-atmosphere interaction over complex topography has a significant influence on rainfall intensity, especially in the heavy precipitating region where the interaction is strong.Without direct coupling between the land surface and the atmosphere in the Prescribed experiment, the diurnal intensity is suppressed by 50%over whole Taiwan and 70% for East Taiwan. Our findings demonstrate that the intensity of the afternoon thunderstorm is sensitive to fast land-atmosphere interactions by modifying local circulation in the mountainous region of Taiwan.

Prognostic value of metabolic tumor volume and total lesion glycolysis from 18F-FDG PET/CT in lymph node metastases and risk stratification of endometrial carcinoma

Dou-dou Liu,Jian-fang Li,Xiaomao Li,Liangjun Xie,Luping Qin,Fangyu Peng,Mu-hua Cheng 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.6

Objective: To investigate the prognostic value of metabolic tumor volume (MTV) and totallesion glycolysis (TLG), measured by preoperative 18F-fluorodeoxyglucose positron emissiontomography/computed tomography (18F-FDG PET/CT), in risk stratification of patients withendometrial carcinoma (EC). Methods: The patients with pathological diagnosis of EC who underwent preoperative18F-FDG PET/CT imaging were retrospectively selected for analysis of the prognostic valuesof PET parameters in risk classification and lymph node metastases (LNMs). Receiveroperating-characteristic analysis was used to analyze the correlation of PET parameters cutoffvalues with deep myometrial invasion (MI), lymphovascular space involvement and LNM forprognostic values in risk stratification. Results: The sensitivity, specificity, positive predictive value, negative predictive value andaccuracy for detection of LNM are 83.3%, 99.7%, 90.9%, 99.5% and 99.2%, respectively. TheMTV and TLG of primary lesion of EC in the patients with LNM are notably higher than thosein patients without LNM, p<0.010. The MTV and TLG of the EC primary lesions in high-riskpatients are significantly higher than those in low-risk patients (p<0.010), but the maximumstandardized uptake value (SUVmax) is not. The MTV and TLG of primary lesions weresuperior to SUVmax for predicting of deep MI, LNM and high-risk of EC (p<0.005). Conclusion: MTV and TLG of primary lesions are more valuable in predicting riskstratification of EC patients. Preoperative 18F-FDG PET/CT imaging is useful in predictingthe LNM of EC and may help guide pelvic lymphadenectomy to avoid unnecessary pelviclymphadenectomy in EC patients with low-risk stratification.