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        Flower cultivation regimes aff ect apocarotenoid accumulation and gene expression during the development of saff ron stigma

        Gui-Fen Zhou,Li-Qin Li,Jiemiao Lu,Jing Li,Chong Yao,Peng Sun,Kaohua Liu,Yan Dong,Luping Qin,Xiao-Dong Qian 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.3

        The quality of commercial saff ron largely depends on apocarotenoid levels. Whether they are aff ected by diff erent fl owercultivation regimes remains unknown. In present study, apocarotenoid levels and the expression profi les of 12 genes relatedto apocarotenoid biosynthesis between fi eld- and indoor-cultivated Crocus sativus L. were analyzed during stigma development. Under both cultivation regimes, apocarotenoid accumulation and gene expression showed similar profi les, fi rstsharply increasing and then gradually decreasing with stigma development, which suggested the coordinated function ofgene expression and apocarotenoid accumulation. However, the rate of increase/decrease varied widely between two blossomregimes at diff erent developmental stages. Based on correlation analysis, βCH1 and ZDS seemed to play a more crucial rolein apocarotenoid accumulation under both cultivation regimes. At anthesis, the total apocarotenoid level in saff ron cultivatedin the fi eld was higher than that cultivated indoors. This may be due to lower gene expression levels in the apocarotenoidpathway, especially βCH1 , ZDS , and CCD2 , and higher CCD4a and NCED expression in the competitive pathway in saff roncultivated indoors than that in the fi eld, which indicated higher conversion in apocarotenoid biosynthesis fl ux from crocinsand picrocrocin synthesis to β-ionone and the ABA biosynthetic branch in saff ron cultivated indoors. These results providevaluable information for modifying planting mode to enhance saff ron quality.

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        Prognostic value of metabolic tumor volume and total lesion glycolysis from 18F-FDG PET/CT in lymph node metastases and risk stratification of endometrial carcinoma

        Dou-dou Liu,Jian-fang Li,Xiaomao Li,Liangjun Xie,Luping Qin,Fangyu Peng,Mu-hua Cheng 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.6

        Objective: To investigate the prognostic value of metabolic tumor volume (MTV) and totallesion glycolysis (TLG), measured by preoperative 18F-fluorodeoxyglucose positron emissiontomography/computed tomography (18F-FDG PET/CT), in risk stratification of patients withendometrial carcinoma (EC). Methods: The patients with pathological diagnosis of EC who underwent preoperative18F-FDG PET/CT imaging were retrospectively selected for analysis of the prognostic valuesof PET parameters in risk classification and lymph node metastases (LNMs). Receiveroperating-characteristic analysis was used to analyze the correlation of PET parameters cutoffvalues with deep myometrial invasion (MI), lymphovascular space involvement and LNM forprognostic values in risk stratification. Results: The sensitivity, specificity, positive predictive value, negative predictive value andaccuracy for detection of LNM are 83.3%, 99.7%, 90.9%, 99.5% and 99.2%, respectively. TheMTV and TLG of primary lesion of EC in the patients with LNM are notably higher than thosein patients without LNM, p<0.010. The MTV and TLG of the EC primary lesions in high-riskpatients are significantly higher than those in low-risk patients (p<0.010), but the maximumstandardized uptake value (SUVmax) is not. The MTV and TLG of primary lesions weresuperior to SUVmax for predicting of deep MI, LNM and high-risk of EC (p<0.005). Conclusion: MTV and TLG of primary lesions are more valuable in predicting riskstratification of EC patients. Preoperative 18F-FDG PET/CT imaging is useful in predictingthe LNM of EC and may help guide pelvic lymphadenectomy to avoid unnecessary pelviclymphadenectomy in EC patients with low-risk stratification.

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