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최재영(Jae Young Choi),이무형(Mu Hyoung Lee),허충림(Choong Rim Haw) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.4
Phakomatosis pigmentovascularis was first reported in 1947 as adiease complex with a distinct association of pigmentary nevus such as nevus of Ota, nevus of Ito, bilon golian spots, nevus spilus and cutaneous hemangioma such as nevus flammeus, which wss assoeisted with systemic organ involvment, We report a case of phakomatosis pigmentovascularis in a 11-year-old girl who had nevus flammeus, nevus spilus, and n.evus of Ota over various parts of her bo by since birth. She was also associated with leg-length discrepancy, but other systemic involvement was absent, (Kor J Dermatol 1994; 32(4): 707-712)
최천필 ( Chun Pill Choi ),김낙인 ( Nack In Kim ),허충림 ( Choong Rim Haw ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.7
Cutaneous larva migrans (CLM) is a rare serpiginous creeping eruption caused by accidental penetration and migration in the skin with a larval form of nematodes. It is caused by hookworm larvae, which are present in the feces of infected dogs and cats. Most cases of CLM in Korea have been shown to involve travelers returning from tropical areas or hot climates. We experienced four patients who had pruritic serpiginous linear eruptions on their skin after traveling to the endemic areas (Boracay island in Philippine). After treatment with albendazole, the skin lesions resolved with post-inflammatory hyperpigmentation. We report herein these four cases, together with a literature review of CLM cases in Korea. (Korean J Dermatol 2006;44(7):843~846)
유한동(Han Dong Yoo),이무형(Mu Hyoung Lee),최중명(Joong Myung Choi),허충림(Choong Rim Haw) 대한피부과학회 1994 大韓皮膚科學會誌 Vol.32 No.4
Background : Chrome has been known to be a toxic and poten sensitizer and is contained in materials with which we are in frequent contact. But we have few reports detailing chrome contact dermatitis by clothes or the chrome content of materials in out use. Objectivee : Our purpose was to evaluate the chrome content in rassieres. Methode : We analyzed chrome in brassieres by using atomied absorption spectrophotometer, quantitatively and diphenylcarbazide method, qualitatively. Results : The content of chrome in a 24 hour saline extracted g roup, a 48 hour saline extracted group and a pure nitric acid extracted group in the black was more abundant than in the reds or in the whites. But there was no aignificant difference in the different fabrics of the blacks. Conclusion : Although, in reality, it is hard to change chrome to an other substrate as the mordant of dyes, a limitation needed to prevent diseases induced by chrome during the process of dyeing. If someone has a sensitivity to chrome, it is necessary to wash new clothes before waring and to avoid black colored underwear. (Kor J Oermatol 19S4; 32(4)': 620-625)
향신재료를 이용한 Oleoresin제조에 관한 연구 - 1. 고추 Oleoresin의 추출
배태진(Tae-Jin Bae),최옥수(Ok-Soo Choi),박재림(Jae-Rim Bahk),김무남(Mu-Nam Kim),한봉호(Bong-Ho Han) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.6
고추의 효율적인 이용의 한 방안으로서 고추 oleoresin추출조건의 구명(究明)과 저장중의 품질변화에 대해서 실험하였다. 고추 oleoresin의 최적 추출용매로는 사용한 8가지 용매중에서 acetone이 가장 좋았으며, 분쇄한 건조고추의 입자크기는 100 mesh, 시료중량에 대한 용매의 혼합비율은 1 : 3(w/w) 및 추출온도는 25℃에서 가장 효과적으로 추출되었다. 추출수율을 기준으로 적정 추출시간은 5시간이였으며, 이상의 최적 추출조건에서의 oleoresin 수율은 18.7%였다. To improve the utilization of red pepper, the extracting conditions of oleoresin, such as kind of solvents, particle sizes of a sample, sample to solvent ratios, extracting temperatures and times, were studied. Among eight solvents used for oleoresin extraction from red pepper, the optimal solvent was acetone. The most appropriate particle size of red pepper powder, extracting temperature and mixing ratio of red pepper to acetone were 100 mesh, 25℃ and 1 to 3(w/w), respectively. The basis of yield in oleoresin extraction, optimum extracting time was about 5 hours. The yield of oleoresin under the above-mentioned conditions was 18.7%.
High-yield Purification and Characterization of Recombinant Human Leukotactin-1 in Pichia pastoris
정경환,윤엽,박두홍,In Hwan Lim,Kong Ju Lee,Eun Kyoung Lee,Mu Rim Choi,Gue-Wha Lee 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.1
The human chemokine, the short version of leukotactin-1 (shLkn-1; molecular weight = 7.2 kD and 66 amino acids), was expressed and secreted into a culture medium using the methylotrophic yeast, Pichia pastoris. The recombinant shLkn-1 was purified from the culture supernatant using a simple two-step procedure consisting of cation exchange and reverse phase chromatography (RPC), in which shLkn-1 was highly purified (99.5%) with a high recovery yield of 82.7%. The C-terminal truncated derivative of shLkn-1 was found in the supernatant and was separated by RPC. The physicochemical properties of the purified shLkn-1 were verified to be the same as expected. The biological activity of the purified recombinant shLkn-1 was also quantified using a chemotaxis assay. It was observed that the recombinant shLkn-1 had the maximum migration activity at a concentration of 10 nM, as potent as MIP-1.