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한국 재래산양에서 Bovine Viral Diarrhea, Bovine Herpesvirus-1, Parainfluenza Type-3 바이러스에 대한 혈청학적 연구
한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3
The 948 goat sera were serologically tested for ten pathogens including bovine viral diarrhea (BVD), bovine herpes virus type-1 (BHV-1) and parainfluenza virus type-3 (PI-3). By viral neutralizaion test, three pathogens including BVD virus, BHV-1, PI-3 were applied for screening each neutralizing antibody and its titration. Among 948 sera, there were 215 seropositives (22.7%) against BVD virus, 25 (2.6%) against BHV-1 virus, and 161 (17.0%) against PI-3 virus, respectively. In the seropositive cases to BVD virus, provincial prevalence of them were recorded as Gyeongnam(30.8%) for the highest one, while as Jeonbuk (15.8%) and Gyeongbuk(l6.7%) relatively for the lower ones. Among them, higher than 1:8 in antibody titration was classified as 74 cases (34.4%) which were suspected the cycle of its infection in their herds. Among the seropositives to BHV-1, it was relatively lower prevalence rate ranging from 1.7% to 6.7% that those of BVD virus and PI-3, but more importantly they were prevailing across the country. Most of the seropositive cases (42.9%) to PI-3 were classified as 1:4-1:16 in titration, but also distributed all around the country. The prevalence in goats varied according to areas (15.8 to 30.8%). Related to the provincial prevalence of three viral agents in Korean goats, it seemed that three viral pathogens would be directly or indirectly involving in various disorders as primary or secondary causative agents resulting in decreased productivity.
한국 재래 산양에서 Leptospira spp.에 대한 혈청역학적 연구
한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3
Between January 1999 and September 2001, 948 sera (120 from each province except 108 from Chungbuk) from 8 provinces were sampled from Korean goats. All the samples were serologically tested for Leptospira spp. By the microscopic agglutination test against four L. interrogans serovars including canicola, icterohaemorrhagiae, pomona and hardjo, 948 Korean goats were confirmed as 11 seropositives (1.2%) only infected with L. icterohemorrhagiae. All positive sera were collected from Gyeonggi (5), Gangwon (4) and Chungbuk (2). Titration of them were classified as 1:100 of 10 sera except one case with 1:200 from Gyeonggi. It was meaningful that the pathogen in Korean goats was mainly distributed in the northen part of the country. Consequently, the results in the study were confirmed and analyzed the recent status of infection of leptospirosisas zoonotic agentsome in Korean goats.
( Jin San Moon ),( Keum Chan Jang ),( Suk Chan Jung ),( Yi Seok Joo ),( Ji Ho Kim ),( Sung Hwan Wee ),( Hye Cheong Koo ),( Yong Ho Park ) 한국수의공중보건학회 2012 예방수의학회지 Vol.36 No.3
The current standard solutions for somatic cells used for calibration of electronic somatic cell counts as reference material in raw milk are preserved with bronopol, boric acid, sodium azide, or potassium dichromate, and have a shelf-life of only up to 6 days at 4 ± 2℃. In the present study, a set of somatic cell standard solutions (SCSS) with a stability of 5 months for calibration of electronic instruments was developed. Somatic cells collected from cow`s milk and stored in a bulk tank at a dairy plant were treated with 10% formaldehyde in order to improve stability, and then separated by centrifugation. The resulting somatic cell suspension was preserved with glycerin, thimerosal, and dimethyl sulfoxide, and diluted in 3% processed skim milk solution ranging from 200,000~250,000 (low level), 350,000~450,000 (medium level), and 550,000~650,000 (high level) cells/㎖. Each SCSS was verified by direct microscope somatic cell counting (DMSCC), C-reader, and commercial standard samples. The average somatic cell count determined by DMSCC was 248, 214, 226 × 103 cells/㎖, 436, 382, 420 × 103 cells/㎖, and 612, 595, 609 × 103 cells/㎖. The coefficient of variation representing the repeatability of DMSCC decreased as the number of cells increased, and was <10.0% in almost all SCSS samples (range 4.6~7.1%). No statistically significant difference in somatic cell concentration was observed after storage at refrigeration temperature (2~6℃) over a period of 22 weeks (5 months). The stabilized SCSS may be useful as a reference material for determination of somatic cell count and quality control in testing of bovine raw milk.
젖소에서 유성분 분석을 통한 영양상태 평가 및 건강관리에 관한 연구 I. 우유중 단백질 및 요소태질소 분석시 변화 요인
문진산(Jin San Moon),주이석(Yi Seok Joo),임숙경(Suk Kyung Im),장금찬(Gum Chan Jang),윤용덕(Yong Dhuk Yoon),위성환(Sung Hwan Wee),이보균(Bo Kyeun Lee),박용호(Young Ho Park),손창호(Chang Ho Son) 한국예방수의학회 2000 예방수의학회지 Vol.24 No.2
Milk urea nitrogen(MUN) and milk protein(MP) are increasingly used as indicators of the protein-energy balance, the actual farm feeding practice and the related nutritional disorders. The main purpose of this study was to investigate the factors affecing on the MUN and MP concentration by sampling procedures, condition of mastitis and addition of preservatives such as potassium dichromate and azidiol. MUN and MP contents were determined using automated infrared procedures. The association between morning and evening MP and MUN contents was determined in 83 individual cow samples from different herds. The mean±standard deviation of morning MUN concentration (17.4±2.1mg/dl) in the 6 herds was similar to that of evening MUN concentration of the lactating cows from the same herd. A significant relationship between morning and evening milk samples of these parameters was found, r=0.93. However differences in MUN level by sampling procedures and addition of presevative such as 0.2% potassium dichromate were observed. A high somatic cell over 3,000,000 in individual cow milk was generally associated with a low MUN value. Thereby, the difference of MUN level on clinical mastitic milk was observed. Therefore, these non-nutritional factors need to be considered when MUN and MP analysis is used for gross diagnosis of protein-energy balance and feeding mangement practice at the farm level. 우유중 요소태질소(MUN) 분석시 검사결과에 영향을 줄 수 있는 여러 요인 즉, 시료 채취방법과 시기, 분석시 원유처리 온도, 보존제 종류, 유방염 감염유무에 따른 영향 등을 비교ㆍ조사하기 위하여 경기도 등 전국소재 951개 목장 27,597개의 분방별, 개체별 우유를 대상으로 유성분을 측정하였다. 우유 채취시기 즉 아침ㆍ저녁우유간의 차이는 거의 없었으나, 첫 젖과 끝 젖 사이의 차이는 매우 심한 것으로 조사되어 시료 채취는 종합우유가 매우 바람직한 것으로 조사되었다. 또한 체세포수가 300만 이상의 원유는 낮은 MUN 수준을 보였으며, 보존제가 처리되지 않는 우유에 비하여 potassium dichromate 첨가시는 대조군보다 평균 3mg/dl의 높은 결과를 나타내어 MUN 검사시 보존제로 potassium dichromate를 사용할 경우에는 결과 판독시 보정 factor를 설정하여 사용해야 함을 고려해야 할 것이며, 보존제로는 azidiol이 좀 더 적합한 것으로 나타났다. 이와 같이 젖소의 영양상태를 평가하기 위해 유단백질과 MUN를 분석할 때에는 정확한 기기 보정과 올바른 시료채취 및 결과해석이 필요로 한다.