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Protein sialylation by sialyltransferase involves radiation resistance.
Lee, Minyoung,Lee, Hae-June,Bae, Sangwoo,Lee, Yun-Sil American Association for Cancer Research 2008 Molecular cancer research Vol.6 No.8
<P>Previously, we identified beta-galactoside alpha(2,6)-sialyltransferase (ST6Gal I) as a candidate biomarker for ionizing radiation. The expression of ST6Gal I and the level of protein sialylation increased following radiation exposure in a dose-dependent manner. Radiation induced ST6Gal I cleavage and the cleaved form of ST6Gal I was soluble and secreted. Sialylation of integrin beta1, a glycosylated cell surface protein, was stimulated by radiation exposure and this increased its stability. Overexpression of ST6Gal I in SW480 colon cancer cells that initially showed a low level of ST6Gal I expression increased the sialylation of integrin beta1 and also increased the stability of the protein. Inhibition of sialylation by transfection with neuraminidase 2 or neuraminidase 3 or by treatment with short interfering RNA targeting ST6Gal I reversed the effects of ST6Gal I overexpression. In addition, ST6Gal I overexpression increased clonogenic survival following radiation exposure and reduced radiation-induced cell death and caspase 3 activation. However, removal of sialic acids by neuraminidase 2 or knockdown of expression by short interfering RNA targeting ST6Gal I restored radiation-induced cell death phenotypes. In conclusion, radiation exposure was found to increase the sialylation of glycoproteins such as integrin beta1 by inducing the expression of ST6Gal I, and increased protein sialylation contributed to cellular radiation resistance.</P>
S-58 H. pylori infection is associated with subclinical coronary atherosclerosis in healthy subjects
( Minyoung Lee ),( Haeri Baek ),( Jong Suk Park ),( Sohee Kim ),( Chanhee Kyung ),( Sangbae Lee ),( Jihong You ),( Su Jung Baik ),( Byoung Kwon Lee ),( Jie-hyun Kim ),( Chul Woo Ahn ),( Kyung Rae Kim 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Background/Aims: Helicobacter pylori (H. pylori) is a gastrointestinal pathogen known to be associated with cardiovascular disease (CVD). However, most reports about the effect of H. pylori on CVD were investigated in patients with a previous history of CVD and little is known in healthy subjects. We evaluated the association between H. pylori infection and subclinical atherosclerosis using cardiac multidetector computed tomography (MDCT) in healthy subjects without CVD history. Methods: From December 2007 to February 2014, 471 subjects who underwent all of rapid urease test (CLO test), pulse-wave velocity (PWV) and MDCT for health check-up were enrolled. H. pylori infection was defined by CLO test positivity on endoscopic gastric biopsy. Significant coronary artery stenosis was defined as ≥50% stenosis in any major epicardial coronary vessel on MDCT. Results: The CLO-positive subjects had a lower high density lipoprotein-cholesterol (HDL-cholesterol) level than the CLO-negative subjects. Although there was no difference in the mean value of PWV, the incidence of significant coronary stenosis was higher in CLO-positive group (7.9% versus 2.9%, p=0.01). Also, the number of subjects with coronary artery calcium score>0 and Log{(Number of segments with plaque)+1} were slightly higher in CLO-positive group. CLO-positive group was 3-fold more likely to have significant coronary artery stenosis even after adjusting confounding factors (adjusted OR 3.081, 95% confidence interval 1.169-8.119, p=0.02). Conclusions: In a healthy population, current H. pylori infection was associated with significant subclinical coronary artery stenosis. The causal effect of H. pylori on subclinical atherosclerosis in a ‘healthy’ population remains to be investigated further.
Lee, Ga-Hang,Yoo, Ki-Chun,An, Yoojeong,Lee, Hae-June,Lee, Minyoung,Uddin, Nizam,Kim, Min-Jung,Kim, In-Gyu,Suh, Yongjoon,Lee, Su-Jae Nature Publishing Group UK 2018 Oncogene Vol.37 No.14
<P>Basal type breast cancer is the most aggressive and has mesenchymal features with a high metastatic ability. However, the signaling node that determines the basal type features in breast cancer remains obscure. Here, we report that FYN among SRC family kinases is required for the maintenance of basal type breast cancer subtype. Importantly, FYN enhanced NOTCH2 activation in basal type breast cancer cells through STAT5-mediated upregulation of Jagged-1 and DLL4 NOTCH ligands, thereby contributed to mesenchymal phenotypes. In addition, we found that high levels of FYN persist in basal type breast cancer cells by a positive feedback loop between FYN and STAT5. FYN interacted directly with STAT5 and increased p-STAT5 that further acts as a transcription factor for FYN. Taken together, our findings demonstrate a pivotal role of FYN and its downstream effectors in maintaining the basal type features in breast cancer.</P>
Therapeutic Potential of Stem Cells Strategy for Cardiovascular Diseases
Lee, Chang Youn,Kim, Ran,Ham, Onju,Lee, Jihyun,Kim, Pilseog,Lee, Seokyeon,Oh, Sekyung,Lee, Hojin,Lee, Minyoung,Kim, Jongmin,Chang, Woochul Hindawi Publishing Corporation 2016 Stem cells international Vol.2016 No.-
<P>Despite development of medicine, cardiovascular diseases (CVDs) are still the leading cause of mortality and morbidity worldwide. Over the past 10 years, various stem cells have been utilized in therapeutic strategies for the treatment of CVDs. CVDs are characterized by a broad range of pathological reactions including inflammation, necrosis, hyperplasia, and hypertrophy. However, the causes of CVDs are still unclear. While there is a limit to the currently available target-dependent treatments, the therapeutic potential of stem cells is very attractive for the treatment of CVDs because of their paracrine effects, anti-inflammatory activity, and immunomodulatory capacity. Various studies have recently reported increased therapeutic potential of transplantation of microRNA- (miRNA-) overexpressing stem cells or small-molecule-treated cells. In addition to treatment with drugs or overexpressed miRNA in stem cells, stem cell-derived extracellular vesicles also have therapeutic potential because they can deliver the stem cell-specific RNA and protein into the host cell, thereby improving cell viability. Here, we reported the state of stem cell-based therapy for the treatment of CVDs and the potential for cell-free based therapy.</P>
Intercepted Hitchhiking Moths through International Vessel at the Korean Port of Entry
Minyoung Kim,Heung-Sik Lee,Jong-Ho Lee,Ki-Jeong Hong,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
Six unknown hitchhiking moths intercepted at the Korean port of entry were detected from an international vessel by the personnels of the International Plant-Quarantine Accreditation Board (IPAB) in 2012. Among them, there was a noctuid moth, Noctua pronuba (Linnaeus), which is a quarantine pest in Korea. Other five species are three erebids (Lophocampa maculata Harris, Lemyrarhodo philodes (Hampson), and Trigonodes cephise (Cramer)), one sphingid (Hippotion rosetta Swinhoe), and one zygaenid (Histia flabellicornis nigrinus Jordan). They are mostly distributed in the subtropical regions. In addition to these hitchhiking moths from the vessel, other interception records from inanimate pathways are provided here on the bases of Pest Information System, Animal and Plant Quarantine Agency (QIA). Thus, it is necessary to monitor inanimate pathways and to adopt comprehensive border surveillance system, in order to protect further invasions of alien insect species.
Soluble form of ST6Gal is critical for radiation-induced migration of colon cancer cells
Minyoung Lee,Jung-Jin Park,Yeung Bae Jin,Yoon-Jin Lee,Young-Gyu Ko,Yun-Sil Lee 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
The β-galactoside α 2,6-sialyltransferase (ST6Gal I) is an enzyme that adds sialic acids to N-linked oligosaccharides of glycoproteins. It is highly expressed in tumors, which probably the basis for the increase of α (2, 6)-sialylation found in cancer cells. Previously we found that ionizing radiation (IR) increased the expression of ST6Gal I, cleaved it into catalytically active form as comparable to enzyme localized in golgi, and these soluble forms were secreted into the culture media. Induction of ST6Gal I significantly increasedthe adhesion and migration of colon cancer cells via sialylation of integrin β1 that is mostlyrelated to cell adhesion and migration. In this study, we further investigated the function of ST6Gal I underlying cleavage, solubilization and release from the cells, especially focusing on the metastasis. ST6Gal I was cleaved by Alzheimer's beta-site amyloid precursor protein-cleaving enzyme (BACE), which was activated by IR, and besides golgi-localized ST6Gal I, soluble form of ST6Gal I was released into the culture media. Although soluble form of ST6Gal I also had sialylation activity, these forms enhanced the migration and invasion of colon cancer cells which was less dependent of intergrin b1 sialylation as compared with golgi-anchored form. Especially, we found that ST6 Gal I repress the E-cadherin promoter activity via induction of Snail. These data suggests that the glycoconjugation with unknown function of soluble sialyltransferase may involve in the process of cancer progression and metastasis which may be in cooperation with golgi-form.