Transcript Profiling of Toll-Like Receptor mRNAs in Selected Tissues of Mink (Neovison vison)

( Mingwei Tong ),( Li Yi ),( Yuening Cheng ),( Miao Zhang ),( Zhigang Cao ),( Jianke Wang ),( Hang Zhao ),( Peng Lin ),( Yong Yang ),( Shipeng Cheng ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.

Practical Integrated Guidance and Control for the High-Speed Anti-Ship Missile to Counter Switching Targets Under a Short Time-to-go Condition

Yunxi Zhang,Yu Fan,Mingwei Sun,Zenghui Wang,Zengqiang Chen 한국항공우주학회 2022 International Journal of Aeronautical and Space Sc Vol.23 No.1

The high-speed anti-ship missile might encounter a challenge in the terminal phase if there are mixed targets, including false and true ones. There is only a quite short time to take action after the onboard radar can distinguish which targets are true or false. In this study, a tunable acceleration feedback gain is used to realize a unique integrated guidance and control scheme in a natural way by maintaining advantages of the traditionally practical proportional navigation and three-loop acceleration autopilot without introducing additional dynamics as the existing methods in the literature. The gain range can be determined using a numerical calculation scheme. The adjoint method is employed to reveal the advantage of the tunable gain and can then obtain the optimal gain. The proposed method can achieve a trade-off between the effective navigation gain and the settling time of the autopilot during the integration. Extensive simulations are performed to validate the appealing discovery. This philosophy offers guideline for the renovation of existing anti-ship missiles.

Improved temperature stability of Ba0.5Sr0.5TiO3/ZnAl2O4 ceramics by controlling microstructure with sintering behavior

Yuze Xue,Mingwei Zhang,Le Xin,Luchao Ren,Panpan Lv,Hang Zhan,Jing He,Jiwei Zhai 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2

0.2ZnAl2O4/0.8Ba0.5Sr0.5TiO3 ceramics with good dielectric temperature stability were synthesized by controlling sinteringbehavior. The relationship between sintering conditions, microstructure, and dielectric properties of ceramics was studied. Cubic structures were confirmed in all ceramics. Double sintering (DS) behavior can effectively improve the density, and thegrain size has no obvious change compared with non-repeated sintering. The maximum dielectric permittivity (220 at 10 kHz)and minimum leakage current (3.98×10-6 A/cm2 at 0.25 kV/cm) are obtained in the DS ceramics, which is superior to thoseof the single sintering ceramics. This can be ascribed to the higher relative density of the DS ceramics in contrast to the singlesintering ceramics. More importantly, the temperature stability of the DS sample in all samples is optimal due to the highsurface energy at grain boundaries and improved density. This work demonstrates a route to produce ceramics with weaktemperature sensitivity for microwave applications.

Enhancement of Photoluminescence by Ag Localized Surface Plasmon Resonance for Ultraviolet Detection

Yanlei Lyu,Jun Ruan,Mingwei Zhao,Ruijin Hong,Hui Lin,Dawei Zhang,Chunxian Tao 한국광학회 2021 Current Optics and Photonics Vol.5 No.1

For higher sensitivity in ultraviolet (UV) and even vacuum ultraviolet (VUV) detection of siliconbased sensors, a sandwich-structured film sensor based on Ag Localized Surface Plasmon Resonance (LSPR) was designed and fabricated. This film sensor was composed of a Ag nanoparticles (NPs) layer, SiO 2 buffer and fluorescence layer by physical vapour deposition and thermal annealing. By tuning the annealing temperature and adding the SiO 2 layer, the resonance absorption wavelength of Ag NPs matched with the emission wavelength of the fluorescence layer. Due to the strong plasmon resonance coupling and electromagnetic field formed on the surface of Ag NPs, the radiative recombination rate of the luminescent materials and the number of fluorescent molecules in the excited state increased. Therefore, the fluorescent emission intensity of the sandwich-structured film sensor was 1.10–1.58 times at 120–200 nm and 2.17–2.93 times at 240–360 nm that of the single-layer film sensor. A feasible method is provided for improving the detection performance of UV and VUV detectors.

PFP@PLGA/Cu12Sb4S13-mediated PTT ablates hepatocellular carcinoma by inhibiting the RAS/MAPK/MT-CO1 signaling pathway

Dong Tianxiu,Jiang Jian,Zhang Hao,Liu Hongyuan,Zou Xiaomeng,Niu Jiamei,Mao Yingxuan,Zhu Mingwei,Chen Xi,Li Zizhuo,Chen Yaodong,Shi Chunying,Yang Xiuhua 나노기술연구협의회 2021 Nano Convergence Vol.8 No.29

Hepatocellular carcinoma (HCC) is one of the most malignant tumors in the world, and patients with HCC face a poor prognosis. The conventional therapeutic strategies for HCC have undergone a challenge-riddled evolution owing to side effects and unsatisfactory efficacy. Here, aiming to provide a new method of HCC elimination, we formulated a novel multifunctional nanocapsule (PFP@PLGA/Cu 12 Sb 4 S 13 , PPCu) with applications in contrast-enhanced ultrasound imaging (CEUS) and photothermal therapy (PTT). These PPCu were successfully constructed with an average diameter of 346 nm (polydispersity index, PDI = 0.276). The reinforced contrast ratio of these PPCu was determined by CEUS, revealing their promising applications in image-guided monitoring of HCC treatment. Furthermore, the excellent photoabsorption and biocompatibility indicated by organ H&E staining indicated that PPCu meet quality expectations for use as photothermal transduction agent (PTA). PPCu treatment at 50 °C and higher temperatures efficiently repressed the proliferation, induced the apoptosis and decreased the motility of HCC cells. These effects might have been results of RAS/MAPK/MT-CO1 signaling pathway inhibition. In summary, PPCu were constructed to integrate CEUS and PTT successfully into therapy, which can lead to HCC elimination through RAS/MAPK/MT-CO1 signaling pathway repression.

A Two-Dimensional Risk Assessment Method for Oil and Gas Pipelines Based on “Accident Probability–Reputation Loss”

Chen Xuefeng,Chen Wentao,Zhang Yifu,Peng Bin,Wang Yuling,Xu Mingwei 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.1

Oil and gas pipeline accidents not only caused personnel casualties, economic losses and environmental pollution but also brought great panic to the public. To provide accurate accident prevention strategies, risk assessment (RA) has been widely used in the oil and gas pipeline industry. It aims to quantify the potential risk due to unexpected eventualities. However, the current RA methods mainly pay attention to the tangible risk (such as the human safety risk, economic risk and environmental risk), and the intangible risk (such as reputation loss risk) is often neglected. This cannot refl ect the real risk for the oil and gas pipeline. To settle existing research limitation, a two-dimensional risk assessment method for oil and gas pipelines based on “accident probability–reputation loss” is proposed. The method mainly involves four steps, namely, risk identifi cation, probability calculation, consequence assessment and risk assessment. The proposed method is used to quantitatively assess a natural gas pipeline in central China. Its results indicate that pipeline risk is unacceptable due to the unacceptability of accident probability and various dimensions of risk. Therefore, this study can enrich and develop the current oil and gas pipeline RA method to refl ect the real risk of oil and gas pipeline accidents.

Fabrication of MnFe2O4 and MnCO3 Nanoparticles Anchored on Amorphous Carbon-Coated Carbon Nanotubes for High-Performance Lithium Batteries and Supercapacitors

Quanwu Wen,Shaohua Zhang,Mingwei Sun,Rencheng Jin 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5

Transition metal oxides as Li-ion batteries (LIBs) anodes have attracted much attention because of their high theoretical capacity. However, the large volume change and low electrical conductivity still hinder their application in LIBs. In this work, a new strategy is proposed to enhance the electrochemical performance by anchoring ultrafine MnFe2O4 and MnCO3 (~5 nm) on amorphous carbon-coated carbon nanotubes. Benefiting from the unique structure, the electrode displays excellent cyclability with a reversible specific capacity of 1012mAh g -1 at 0.1A g -1 after 100 cycles and outstanding rate performance accompanied by a high specific capacity of 568mAh g -1 at 5A g -1 as anode for lithium batteries. When evaluated as supercapacitors, the electrode delivers the specific capacitance of 588.9 F g -1 at a current density of 1A g -1 and maintains the capacitance retention 94.7% after 4000 cycles at 5A g -1.

SAXS investigations of the fractal character of additive silica xerogels

Fei He,Xiaodong He,Mingwei Li,Sumei Zhang 한양대학교 세라믹연구소 2008 Journal of Ceramic Processing Research Vol.9 No.4

Two-step acid-base catalyzed silica xerogels with different amounts of additives, including TiO2 powder, silica xerogel powders and short glass fibers, were prepared through a sol-gel route and ambient pressure drying. The fractal dimensions of these additive silica xerogels were investigated by the means of small-angle X-ray scattering (SAXS). Specific surface area and the average chord length were calculated by the Debye method. The changes of the porous structure were compared between the pure silica xerogels and the impure ones. The results show that additive silica xerogels do not possess a surface fractal character. The mass fractal range of silica xerogels decreases and colloid particle size increases with an increase of additives. The adding of excessive silica xerogel powders results in agglomeration of particles which goes beyond the metrical limit of SAXS. The mass fractal dimension and fractal range decrease with the addition of additional short fibers. The shrinkage was restrained in the course of drying by adding short fibers Two-step acid-base catalyzed silica xerogels with different amounts of additives, including TiO2 powder, silica xerogel powders and short glass fibers, were prepared through a sol-gel route and ambient pressure drying. The fractal dimensions of these additive silica xerogels were investigated by the means of small-angle X-ray scattering (SAXS). Specific surface area and the average chord length were calculated by the Debye method. The changes of the porous structure were compared between the pure silica xerogels and the impure ones. The results show that additive silica xerogels do not possess a surface fractal character. The mass fractal range of silica xerogels decreases and colloid particle size increases with an increase of additives. The adding of excessive silica xerogel powders results in agglomeration of particles which goes beyond the metrical limit of SAXS. The mass fractal dimension and fractal range decrease with the addition of additional short fibers. The shrinkage was restrained in the course of drying by adding short fibers

In vitro Antioxidant Activities of Proanthocyanidins Extracted from the Lotus Seedpod and Ameliorative Effects on Learning and Memory Impairment in Scopolamine-induced Amnesia Mice

Juan Xiao,Shuyi Li,Yong Sui,Xiaopeng Li,Qian Wu,Ruifen Zhang,Mingwei Zhang,Bijun Xie,Zhida Sun 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4

The antioxidant activity of proanthocyanidins extracted from the lotus seedpods (LSPC) in vitro and ameliorative effects on memory impairment induced using scopolamine in mice were studied. 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging ability, oxygen radical absorbance capacity, and cellular antioxidant activity assays revealed a potent LSPC antioxidant activity. Y-maze and step-down avoidance testing showed that LSPC administration at 30, 60, and 90 mg/kg ameliorated memory impairment. LSPC improved glutathione peroxidase and superoxide dismutase activities, inhibited activities of monoamine oxidase-B, total nitric oxide synthase, neuronal nitric oxide synthase, and acetylcholinesterase, and had no influence on inducible nitric oxide synthase and nitric oxide levels in the brain. LSPC ameliorated scopolamineinduced memory impairment based on improvement of the antioxidant system and cholinergic activity, which may be associated with potent antioxidant ability.

Increased Expression of EMMPRIN and VEGF in the Rat Brain after Gamma Irradiation

Ming Wei,Hong Li,Huiling Huang,Desheng Xu,Dashi Zhi,Dong Liu,Yipei Zhang 대한의학회 2012 Journal of Korean medical science Vol.27 No.3

The extracellular matrix metalloproteinase inducer (EMMPRIN) has been known to play a key regulatory role in pathological angiogenesis. A elevated activation of vascular endothelial growth factor (VEGF) following radiation injury has been shown to mediate blood-brain barrier (BBB) breakdown. However, the roles of EMMPRIN and VEGF in radiation-induced brain injury after gamma knife surgery (GKS) are not clearly understood. In this study, we investigated EMMPRIN changes in a rat model of radiation injury following GKS and examined potential associations between EMMPRIN and VEGF expression. Adult male rats were subjected to cerebral radiation injury by GKS under anesthesia. We found that EMMPRIN and VEGF expression were markedly upregulated in the target area at 8-12 weeks after GKS compared with the control group by western blot,immunohistochemistry, and RT-PCR analysis. Immunofluorescent double staining demonstrated that EMMPRIN signals colocalized with caspase-3 and VEGF-positive cells. Our data also demonstrated that increased EMMPRIN expression was correlated with increased VEGF levels in a temporal manner. This is the first study to show that EMMPRIN and VEGF may play a role in radiation injuries of the central nervous system after GKS.