Purification and Characterization of an Antioxidant Protein from Fertilized Eggs

Shaohua Yang,Zhengjiang Tang,ShanShan Tang,Tingfang Zhang,Fei Tang,Yu Wu,Ying Wang,Lulu Wang,Guoqing Liu 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.6

Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was 57.16ºC. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ·OH assays, and its antioxidant activity was stable at 30-50ºC in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.

Damage detection using the improved Kullback-Leibler divergence

Shaohua Tian,Xuefeng Chen,Zhibo Yang,Zhengjia He,Xingwu Zhang 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.3

Structural health monitoring is crucial to maintain the structural performance safely. Moreover, the Kullback-Leibler divergence (KLD) is applied usually to asset the similarity between different probability density functions in the pattern recognition. In this study, the KLD is employed to detect the damage. However the asymmetry of the KLD is a shortcoming for the damage detection, to overcoming this shortcoming, two other divergences and one statistic distribution are proposed. Then the damage identification by the KLD and its three descriptions from the symmetric point of view is investigated. In order to improve the reliability and accuracy of the four divergences, the gapped smoothing method (GSM) is adopted. On the basis of the damage index approach, the new damage index (DI) for detect damage more accurately based on the four divergences is developed. In the last, the grey relational coefficient and hypothesis test (GRCHT) is utilized to obtain the more precise damage identification results. Finally, a clear remarkable improvement can be observed. To demonstrate the feasibility and accuracy of the proposed method, examples of an isotropic beam with different damage scenarios are employed so as to check the present approaches numerically. The final results show that the developed approach successfully located the damaged region in all cases effect and accurately.

A Cooperative Intrusion Detection Model for Cloud Computing Networks

Shaohua Teng,Chaoyu Zheng,Haibin Zhu,Dongning Liu,Wei Zhang 보안공학연구지원센터 2014 International Journal of Security and Its Applicat Vol.8 No.3

Beijing Natural Gas Supply and Environment Protection

Dong Shaohua,Zhang Hong 한국에너지학회 2008 한국에너지공학회 학술발표회 Vol.2008 No.-

This paper analyses the main cause of pollution in Beijing city-the limiting of the pollution caused by coal burning has seen important progress, and natural gas is assuming an important role in China’s energy strategy as the country actively seeks new and cleaner sources of energy, since 1997 to 2007, the gas supply for Beijing was given, compared with the air quality standard above II which set up by WHO(World Healthy Organization), as a result the important relationship between the environment and gas using was found, then the percent of SO₂, NO₂, PM10, CO, O₃ of air was shown from August 8<SUP>th</SUP> -24<SUP>th</SUP> 2006, Which it was verified that the quality of air will be perfect within the standard of WHO during the Olympic Game 2008 China.

Damage detection using the improved Kullback-Leibler divergence

Tian, Shaohua,Chen, Xuefeng,Yang, Zhibo,He, Zhengjia,Zhang, Xingwu Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.3

Structural health monitoring is crucial to maintain the structural performance safely. Moreover, the Kullback-Leibler divergence (KLD) is applied usually to asset the similarity between different probability density functions in the pattern recognition. In this study, the KLD is employed to detect the damage. However the asymmetry of the KLD is a shortcoming for the damage detection, to overcoming this shortcoming, two other divergences and one statistic distribution are proposed. Then the damage identification by the KLD and its three descriptions from the symmetric point of view is investigated. In order to improve the reliability and accuracy of the four divergences, the gapped smoothing method (GSM) is adopted. On the basis of the damage index approach, the new damage index (DI) for detect damage more accurately based on the four divergences is developed. In the last, the grey relational coefficient and hypothesis test (GRCHT) is utilized to obtain the more precise damage identification results. Finally, a clear remarkable improvement can be observed. To demonstrate the feasibility and accuracy of the proposed method, examples of an isotropic beam with different damage scenarios are employed so as to check the present approaches numerically. The final results show that the developed approach successfully located the damaged region in all cases effect and accurately.

Beijing Natural Gas Supply and Environment Protection

Dong Shaohua,Zhang Hong 한국가스학회 2008 한국가스학회 학술대회논문집 Vol.2008 No.4

Purification and Characterization of an Antioxidant Protein from Fertilized Eggs

Yang, Shaohua,Tang, Zhengjiang,Tang, ShanShan,Zhang, Tingfang,Tang, Fei,Wu, Yu,Wang, Ying,Wang, Lu Lu,Liu, Guoqing Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.6

Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was $57.16^{\circ}C$. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ${\cdot}OH$ assays, and its antioxidant activity was stable at $30-50^{\circ}C$ in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.

Mycobacterium vaccae induces a strong Th1 response that subsequently declines in C57BL/6 mice

Lijiao Zhang,Yanlong Jiang,Ziyin Cui,Wentao Yang,Limin Yue,Yingcong Ma,Shaohua Shi,Chun-fang Wang,Chun-feng Wang,Aidong Qian 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4

Mycobacterium (M.) vaccae is a fast-growing species of saprophytic bacteria that is widely distributed. To understand the host immuneresponses induced by M. vaccae isolated from bovine submaxillary lymph nodes, C57BL/6 mice were infected with reference strain M. bovisBacillus Calmette-Guérin (BCG) and isolated M. vaccae using intraperitoneal injections. Comparison of the bacterial replication and organpathology between M. vaccae and M. bovis BCG revealed that M. vaccae was more malignant than M. bovis in mice. We also demonstratedthat serum from the M. vaccae-infected mice contained a higher expression level of gamma-interferon (IFN-), tumor necrosis factor alpha,monocyte chemoattractant protein-1, interleukin (IL)-4, IL-12, IL-10 and transforming growth factor beta than did the other groups, especiallyafter week 4. Furthermore, when the numbers of CD3+CD4+IFN-+ and CD3+CD4+IL4+ cells in the infected mice were observed by flowcytometry, we found that a powerful T helper 1 (Th1) response was induced by M. vaccae infection, which was associated with the emergenceof CD3+CD4+IFN-+ cells. However, the Th1 response declined over time, which was associated with appearance of the CD4+CD25+FoxP3+and CD4+CD25+CD152+Treg cell reaction. In addition, a strong Th2 response was found. Finally, we found that M. vaccae infection increasedthe production of type I IFNs, which was associated with a reduced Th1 response.

Pd–Fe bimetallic nanoparticles anchored on N‑doped carbon‑modified graphene for efficient catalytic organic reactions

Yue Zhang,Jie Huang,Zixuan Dong,Yu Zhan,Jiangbo Xi,Jian Xiao,Shaohua Huang,Fan Tian 한국탄소학회 2023 Carbon Letters Vol.33 No.1

A promising approach to enhance catalytic performance of supported heterogeneous nano-metal catalysts is to uniformly disperse active nanoparticles on the support. In this work, N-doped carbon-modified graphene (G@NC) nanosheet is designed and prepared to anchor Pd–Fe bimetallic nanoparticles (Pd–Fe/G@NC). The N-doped carbon modification on graphene surface could construct a sandwich-like structure (G@NC), which not only prevented the re-stacking of graphene nanosheets but also provided confined space for stable anchoring of bimetallic Pd–Fe nanoparticles. Benefitted from the unique structural property and synergetic effect of metal Pd and Fe species, the as-obtained Pd–Fe/G@NC composite displays excellent catalytic activity toward 4-nitrophenol reduction reaction with a turnover frequency of 613.2 min? 1, which is far superior to that of the mono-metal counterparts (Fe/G@NC and Pd/G@NC). More importantly, Pd–Fe/G@NC catalyst also exhibits favorable catalytic performance in the reduction of other nitroaromatic compounds (nitrobenzene, 4-nitrotoluene, 4-chloronitrobenzene, and so on). In addition, Pd–Fe/G@NC can catalyze the oxidation of furfuraldehyde to furoic acid with a high yield of 88.64%. This work provides a new guide for rationally designing and developing advanced supported heterogeneous bimetallic catalyst.

Larvicidal activity of <i>Magnolia denudata</i> seed hydrodistillate constituents and related compounds and liquid formulations towards two susceptible and two wild mosquito species

Wang, Zhang‐,qian,Perumalsamy, Haribalan,Wang, Mo,Shu, Shaohua,Ahn, Young‐,Joon John Wiley Sons, Ltd 2016 Pest Management Science Vol.72 No.5

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P><I>Anopheles sinensis</I>, <I>Aedes aegypti</I> and <I>Aedes albopictus</I> and <I>Culex pipiens pallens</I> mosquitoes transmit malaria, dengue fever and West Nile virus diseases respectively. This study was conducted to determine the toxicity of 17 constituents from <I>Magnolia denudata</I> seed hydrodistillate (MD‐SHD) and four experimental MD‐SHD liquid formulations (10–50 mg L<SUP>−1</SUP> liquid) to third‐instar larvae from insecticide‐susceptible <I>Cx. p. pallens</I> and <I>Ae. aegypti</I> as well as wild <I>Ae. albopictus</I> and <I>An. sinensis</I>.</P><P><B>RESULTS</B></P><P>2,4‐Di‐<I>tert</I>‐butylphenol was the most toxic constituent (LC<SUB>50</SUB> 1.98–3.90 mg L<SUP>−1</SUP>), followed by linoleic acid (7.19–10.49 mg L<SUP>−1</SUP>), towards larvae of the four mosquito species. High toxicity was also produced by nerolidol, (±)‐limonene, <I>α</I>‐terpinene and <I>γ</I>‐terpinene (LC<SUB>50</SUB> 9.84–36.42 mg L<SUP>−1</SUP>). The toxicity of these compounds was virtually identical towards larvae of the four mosquito species, even though <I>An. sinensis</I> larvae were resistant to deltamethrin and temephos. The MS‐SHD 50 mg L<SUP>−1</SUP> liquid resulted in 92–100% control towards larvae of the four mosquito species, while commercial temephos 200 g L<SUP>−1</SUP> emulsifiable concentrate was almost ineffective towards <I>An. sinensis</I> larvae (30% mortality).</P><P><B>CONCLUSION</B></P><P>Reasonable mosquito control in the aquatic environment can be achieved by MD‐SHD 50 mg L<SUP>−1</SUP> liquid as a potential larvicide. © 2015 Society of Chemical Industry</P>