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Transcript Profiling of Toll-Like Receptor mRNAs in Selected Tissues of Mink (Neovison vison)
( Mingwei Tong ),( Li Yi ),( Yuening Cheng ),( Miao Zhang ),( Zhigang Cao ),( Jianke Wang ),( Hang Zhao ),( Peng Lin ),( Yong Yang ),( Shipeng Cheng ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12
Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.
Enhancement of Photoluminescence by Ag Localized Surface Plasmon Resonance for Ultraviolet Detection
Yanlei Lyu,Jun Ruan,Mingwei Zhao,Ruijin Hong,Hui Lin,Dawei Zhang,Chunxian Tao 한국광학회 2021 Current Optics and Photonics Vol.5 No.1
For higher sensitivity in ultraviolet (UV) and even vacuum ultraviolet (VUV) detection of siliconbased sensors, a sandwich-structured film sensor based on Ag Localized Surface Plasmon Resonance (LSPR) was designed and fabricated. This film sensor was composed of a Ag nanoparticles (NPs) layer, SiO 2 buffer and fluorescence layer by physical vapour deposition and thermal annealing. By tuning the annealing temperature and adding the SiO 2 layer, the resonance absorption wavelength of Ag NPs matched with the emission wavelength of the fluorescence layer. Due to the strong plasmon resonance coupling and electromagnetic field formed on the surface of Ag NPs, the radiative recombination rate of the luminescent materials and the number of fluorescent molecules in the excited state increased. Therefore, the fluorescent emission intensity of the sandwich-structured film sensor was 1.10–1.58 times at 120–200 nm and 2.17–2.93 times at 240–360 nm that of the single-layer film sensor. A feasible method is provided for improving the detection performance of UV and VUV detectors.
Zhihu Yan,Caili Dai,Mingwei Zhao,Yongpeng Sun,Guang Zhao 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.37 No.-
Through extensive experiments, a reusable viscoelastic surfactant (rVES) fracturing fluid using a selfdesignedand synthesized surfactant was developed. Laboratory performance evaluation results showedthat various performances of rVES fracturing fluid are excellent. The most attractive aspect is the gellingand gel breaking of rVES fracturing fluid was achieved by altering the pH value. The recycled fracturingfluid still maintains good performances through three times cycles, which represents that it has goodprospects for re-use of flowback waters. In addition, large-scale coarse-grained molecular dynamics(CGMD) simulations results clearly revealed the formation mechanism of rVES fracturing fluid at themolecular level.
Yifei Liu,Caili Dai,Kai Wangb,Mingwei Zhao,Guang Zhao,Shuai Yang,Zhihu Yan,Qing You 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.35 No.-
Aiming at water shut-off in high temperature reservoirs, the hydroquinone (HQ)–hexamethylenetetra-mine (HMTA) gel system was studied. The gelation performance and effects of various parameters weresystematically evaluated. With increase of the concentrations and temperature, gelation time decreasesand gel strength increases. In addition, gelation rate and gel strength were enhanced on adding acid. Moreover, the gel system shows good salt tolerance. The thermal stability measurement indicates thatthe gel can maintain stability up to 140 8C. Uniformly distributed three-dimensional network structurewas formed in the gel. At last, the gelation mechanism was proposed, illuminating the detailed gelationprocess clearly.
Synthesis, surface adsorption and micelle formation of a class of morpholinium gemini surfactants
Caili Dai,Sisi Fang,Mei Hu,Xiaojuan He,Mingwei Zhao,Xuepeng Wu,Shuai Yang,Yining Wua 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-
A series of novel gemini surfactants with morpholinium headgroup, 4, 40-(alkane-4, n-40)bis(4-decyl, dodecyl or tetradecyl morpholinium)bromide (Mm-n-Mm, m = 10, 12, 14, n = 3, 4), were synthesized and characterized using 1H NMR. The surfactants were investigated for their adsorption and micellization in aqueous solutions through surface tension and conductivity methods It was found that the physicochemical properties, such as surface activity and thermodynamic property of the aqueous surfactant system, were dependent on the lengths of both hydrophobic tail chain and spacer. With the increase of the hydrophobic chain length or the decrease of spacer length, the cmc value of the surfactant decreases and the ability to reduce the surface tension is stronger. The calculation result of thermodynamic parameters indicated that the surfactants we synthesized are more inclined to aggregate in solution as compared with traditional m-s-m type gemini surfactants, and the micellization of Mm-n-Mm was an entropy-driven process.
CO2-responsive smart wormlike micelles based on monomer and “pseudo” gemini surfactant
Xuepeng Wu,Yongping Huang,Sisi Fang,Caili Dai,Hao Li,Zhongliang Xu,Mingwei Zhao 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.60 No.-
CO2-responsive wormlike micelles based on monomer erucic acid 3-(N,N-dimethylamino)propylamide (EADP) with or without sodium salicylate (NaSal) in aqueous solution were studied and compared with “pseudo” gemini system. The “pseudo” gemini system were formed by a mixture of EADP and maleic acid (MA) with molar ratio between 2:1–1:1. Different morphological transformations of microstructures in response to EADP concentrations, additives and CO2 stimuli were further investigated in these systems, the wormlike micelle or vesicles can be obtained. The mechanism of these transitions were further investigated by rheology, cryo-TEM and DFT calculation, the results showed that the mechanism behind this transition involved reversible reaction of CO2 with EADP. The mechanism in this research may give further evidence to the self-assembly of CO2-responsive surfactants.