Pre-harvest 1-Methylcyclopropene Application Affects Post-harvest Physiology and Storage Life of the Cut Rose cv. Carola

Shuai Huang,Huiling Ma,Bi Gong,Fujuan Wei 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.2

We evaluated and optimized the effects of an alternative pre-harvest application of the non-toxic ethyleneaction inhibitor 1-methylcyclopropene (1-MCP)on the physiology and storage life of the cut rose (Rosa hybrida) cv. Carola. Cut rose was treated with a single pre-harvest liquid formula spray of 10 μL·L-11-MCP or a single treatmentof a modified atmosphere packaging of 50-μm thick polyethylene film (PE50), or a combination of the two (1-MCP+PE50),and the effects on vase life, storage life, and cold storage senescence related physiological changes were evaluated. Cut rose subjected to the pre-harvest 1-MCP spray treatment had the longest storage life (26 days), while those subjectedto PE50, 1-MCP+PE50, and the untreated control treatment had mean storage lives of 22, 19, and 16 days, respectively. Additionally, the pre-harvest 1-MCP spray treatment significantly extended the vase life of cold-stored flowers forzero or six days, by 27.08% and 42.55%, respectively. The pre-harvest spray treatment significantly suppressed respirationintensity, ethylene production, soluble protein degradation, 1-aminocyclopropane-1-carboxylic acid (ACC) production,and ACC oxidase activity. The 1-MCP+PE50 treatment compromised all the effects of the 1-MCP treatment exceptthe effect on soluble protein content. We conclude that a pre-harvest 1-MCP spray treatment has positive effects onthe vase life and storage longevity of cut rose, and that combining this treatment with a PE50 package diminishesthese effects.

Lactiplantibacillus sp. G6 isolated from goose intestine as starter culture for degrading nitrite and improving quality in Chinese pickle fermentation

Ziya Lan,Huiling Huang,Chenyang Liu,Jincai Li,Xueling Li 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.6

Animal intestines is considered as a source of lactic acid bacteria (LAB) that have potential to decrease the nitrite level during fermentation of food such as pickles. It was hypothesized that optimized level of LAB has a high capacity to degrade nitrite during Chinese pickle fermentation and benefit a higher acceptability of the Chinese pickle product. This study aims to investigate the performance of a goose intestine-isolated LAB strain G6 under the species Lactiplantibacillus plantarum as a starter culture of Chinese pickles. The results showed that Lactiplantibacillus sp. G6 had a nitrite degradation rate close to 100% under the MRS broth condition of 25 °C, 2% inoculum volume and pH at 5. As a starter culture for Chinese pickle, this strain was able to achieve a higher LABs amount, lower nitrite residue after fermentation, compared with the group without the starter, which implicates its feasibility of applying on fermented food for reducing nitrite level.

Salp swarm algorithm with iterative mapping and local escaping for multi-level threshold image segmentation: a skin cancer dermoscopic case study

Hao Shuhui,Huang Changcheng,Heidari Ali Asghar,Chen Huiling,Li Lingzhi,Algarni Abeer D.,Elmannai Hela,Xu Suling 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.2

If found and treated early, fast-growing skin cancers can dramatically prolong patients’ lives. Dermoscopy is a convenient and reliable tool during the fore-period detection stage of skin cancer, so the efficient processing of digital images of dermoscopy is particularly critical to improving the level of a skin cancer diagnosis. Notably, image segmentation is a part of image preprocessing and essential technical support in the process of image processing. In addition, multi-threshold image segmentation (MIS) technology is extensively used due to its straightforward and effective features. Many academics have coupled different meta-heuristic algorithms with MIS to raise image segmentation quality. Nonetheless, these meta-heuristic algorithms frequently enter local optima. Therefore, this paper suggests an improved salp swarm algorithm (ILSSA) method that combines iterative mapping and local escaping operator to address this drawback. Besides, this paper also proposes the ILSSA-based MIS approach, which is triumphantly utilized to segment dermoscopic images of skin cancer. This method uses two-dimensional (2D) Kapur’s entropy as the objective function and employs non-local means 2D histogram to represent the image information. Furthermore, an array of benchmark function test experiments demonstrated that ILSSA could alleviate the local optimal problem more effectively than other compared algorithms. Afterward, the skin cancer dermoscopy image segmentation experiment displayed that the proposed ILSSA-based MIS method obtained superior segmentation results than other MIS peers and was more adaptable at different thresholds.

Differential responses of walnut cultivars to cold storage and their correlation with postharvest physiological parameters

Xue Yuan,Shuai Huang,Huiling Ma,Ning Huang,Niu Ye 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3

In this study, we screened eight walnut ( Juglans regia L.) cultivars (‘Xifu2’, ‘Shaanhe’, ‘Weina’, ‘Xiangling’, ‘Xiluo3’,‘Xifu1’, ‘Liaoning4’, and ‘Qingxiang’) in order to establish reliable selection criteria for storage-tolerance. Fresh walnutfruit were stored at 0 ± 0.5 °C and 70–80% relative humidity. Browning index, postharvest physio-biochemical characteristics,acid value, and sensory quality were measured. The results showed that ‘Xifu2’, ‘Shaanhe’, and ‘Weina’ developedbrowning early and quickly and storage was terminated for these cultivars at 60 days. ‘Xiluo3’, ‘Xifu1’, ‘Liaoning4’, and‘Qingxiang’ had a slow browning rate and storage was terminated at 72 days. ‘Xiangling’ developed browning at a mediumrate and storage was terminated at 72 days. Correlation analysis suggested that the terminal browning index of each cultivarpositively correlated with lipoxygenase activity at 18 days of storage, polyphenol oxidase activity at 18–30 days, malondialdehydecontent at 18 days, and the respiration rate at 42 days, while it negatively correlated with the total phenolic contentat 18 days and phenylalanine ammonia lyase activity at 12 days. The storage tolerance of each cultivar was evaluatedaccording to the terminal browning index, acid value, and sensory quality. Comprehensive evaluation and cluster analysiswere performed to rank the cold storage tolerance of the eight cultivars as follows: ‘Qingxiang’ and ‘Xifu1’ > ‘Liaoning4’and ‘Xiluo3’ > ‘Xifu2’ and ‘Xiangling’ > ‘Shaanhe’ and ‘Weina’. In conclusion, cultivars used for fresh in-husk walnut fruitwith lower levels of membrane peroxidation and phenol oxidation, and higher level of phenol synthesis around 18 days ofstorage would be suitable candidates for cold storage. ‘Qingxiang’ and ‘Xifu1’ were selected as storage-tolerant cultivars.

Increased Expression of EMMPRIN and VEGF in the Rat Brain after Gamma Irradiation

Ming Wei,Hong Li,Huiling Huang,Desheng Xu,Dashi Zhi,Dong Liu,Yipei Zhang 대한의학회 2012 Journal of Korean medical science Vol.27 No.3

The extracellular matrix metalloproteinase inducer (EMMPRIN) has been known to play a key regulatory role in pathological angiogenesis. A elevated activation of vascular endothelial growth factor (VEGF) following radiation injury has been shown to mediate blood-brain barrier (BBB) breakdown. However, the roles of EMMPRIN and VEGF in radiation-induced brain injury after gamma knife surgery (GKS) are not clearly understood. In this study, we investigated EMMPRIN changes in a rat model of radiation injury following GKS and examined potential associations between EMMPRIN and VEGF expression. Adult male rats were subjected to cerebral radiation injury by GKS under anesthesia. We found that EMMPRIN and VEGF expression were markedly upregulated in the target area at 8-12 weeks after GKS compared with the control group by western blot,immunohistochemistry, and RT-PCR analysis. Immunofluorescent double staining demonstrated that EMMPRIN signals colocalized with caspase-3 and VEGF-positive cells. Our data also demonstrated that increased EMMPRIN expression was correlated with increased VEGF levels in a temporal manner. This is the first study to show that EMMPRIN and VEGF may play a role in radiation injuries of the central nervous system after GKS.

Pre-harvest ethylene control affects vase life of cut rose ‘Carola’ by regulating energy metabolism and antioxidant enzyme activity

Bi Gong,Shuai Huang,Niu Ye,Xue Yuan,Huiling Ma 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.6

We studied the role of ethylene control in regulating energy metabolism, antioxidant enzyme activity, and vase life of cutrose Rosa hybrida ‘Carola’. Rose fl owers at stage II were sprayed with one of the following solutions: water (control), 10μL L −1 1-methylcyclopropene (1-MCP), or 0.5 g L −1 2-chloroethanephosphonic acid (ethephon). After harvest, ethyleneproduction rate, respiration intensity, energy charge (EC), activities of energy metabolism-related and antioxidant enzymes,and malondialdehyde (MDA) content were measured. Results showed that 1-MCP enhanced the activities of superoxidedismutase, H + -adenosine triphosphatase, Ca 2+ -adenosine triphosphatase, succinic dehydrogenase, and cytochrome c oxidase,increased adenosine triphosphate (ATP) content, maintained high EC levels, inhibited respiration intensity, reduced peroxidase(POD) and polyphenol oxidase (PPO) activity and MDA accumulation, and prolonged vase life. Ethephon promotedethylene production and respiration intensity, increased POD and PPO activity, reduced ATP content and EC levels, andaccelerated senescence. Our results support a novel role for ethylene control in regulating senescence of ‘Carola’.