Accuracy of cardiac output measurements during off-pump coronary artery bypass grafting: according to the vessel anastomosis sites

Sung Yong Park,Dae Hee Kim,Han Bum Joe,Ji Young Yoo,Jin Soo Kim,Min Kang,Yong Woo Hong 대한마취통증의학회 2012 Korean Journal of Anesthesiology Vol.62 No.5

Background: During beating heart surgery, the accuracy of cardiac output (CO) measurement techniques may be influenced by several factors. This study was conducted to analyze the clinical agreement among stat CO mode (SCO), continuous CO mode (CCO), arterial pressure waveform-based CO estimation (APCO), and transesophageal Doppler ultrasound technique (UCCO) according to the vessel anastomosis sites. Methods: This study was prospectively performed in 25 patients who would be undergoing elective OPCAB. Hemodynamic variables were recorded at the following time points: during left anterior descending (LAD) anastomosis at 1 min and 5 min; during obtuse marginal (OM) anastomosis at 1 min and 5 min: and during right coronary artery (RCA) anastomosis at 1 min and 5 min. The variables measured including the SCO, CCO, APCO, and UCCO. Results: CO measurement techniques showed different correlations according to vessel anastomosis site. However, the percent error observed was higher than the value of 30% postulated by the criteria of Critchley and Critchley during all study periods for all CO measurement techniques. Conclusions: In the beating heart procedure, SCO, CCO and APCO showed different correlations according to the vessel anastomosis sites and did not agree with UCCO. CO values from the various measurement techniques should be interpreted with caution during OPCAB. Background: During beating heart surgery, the accuracy of cardiac output (CO) measurement techniques may be influenced by several factors. This study was conducted to analyze the clinical agreement among stat CO mode (SCO), continuous CO mode (CCO), arterial pressure waveform-based CO estimation (APCO), and transesophageal Doppler ultrasound technique (UCCO) according to the vessel anastomosis sites. Methods: This study was prospectively performed in 25 patients who would be undergoing elective OPCAB. Hemodynamic variables were recorded at the following time points: during left anterior descending (LAD) anastomosis at 1 min and 5 min; during obtuse marginal (OM) anastomosis at 1 min and 5 min: and during right coronary artery (RCA) anastomosis at 1 min and 5 min. The variables measured including the SCO, CCO, APCO, and UCCO. Results: CO measurement techniques showed different correlations according to vessel anastomosis site. However, the percent error observed was higher than the value of 30% postulated by the criteria of Critchley and Critchley during all study periods for all CO measurement techniques. Conclusions: In the beating heart procedure, SCO, CCO and APCO showed different correlations according to the vessel anastomosis sites and did not agree with UCCO. CO values from the various measurement techniques should be interpreted with caution during OPCAB.

Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme

Kang, Wonchull,Hong, Se Hoon,Lee, Hye Min,Kim, Na Yeon,Lim, Yun Chan,Le, Le Thi My,Lim, Bitna,Kim, Hyun Chul,Kim, Tae Yeon,Ashida, Hiroki,Yokota, Akiho,Hah, Sang Soo,Chun, Keun Ho,Jung, Yong-Keun,Yang National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.1

<P>APIP, Apaf-1 interacting protein, has been known to inhibit two main types of programmed cell death, apoptosis and pyroptosis, and was recently found to be associated with cancers and inflammatory diseases. Distinct from its inhibitory role in cell death, APIP was also shown to act as a 5-methylthioribulose-1-phosphate dehydratase, or MtnB, in the methionine salvage pathway. Here we report the structural and enzymatic characterization of human APIP as an MtnB enzyme with a <I>K</I><SUB><I>m</I></SUB> of 9.32 μM and a <I>V</I><SUB><I>max</I></SUB> of 1.39 μmol min<SUP>−1</SUP> mg<SUP>−1</SUP>. The crystal structure was determined at 2.0-Å resolution, revealing an overall fold similar to members of the zinc-dependent class II aldolase family. APIP/MtnB exists as a tetramer in solution and exhibits an assembly with <I>C4</I> symmetry in the crystal lattice. The pocket-shaped active site is located at the end of a long cleft between two adjacent subunits. We propose an enzymatic reaction mechanism involving Glu139* as a catalytic acid/base, as supported by enzymatic assay, substrate-docking study, and sequence conservation analysis. We explored the relationship between two distinct functions of APIP/MtnB, cell death inhibition, and methionine salvage, by measuring the ability of enzymatic mutants to inhibit cell death, and determined that APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis. Our results establish the structural and biochemical groundwork for future mechanistic studies of the role of APIP/MtnB in modulating cell death and inflammation and in the development of related diseases.</P>

Conditioned Media of ASC-17D Sertoli Cells Induce G1-Growth Arrest of DU145 Human Prostate Cancer Cells

Min, Bon Hong,Kang, Sang Wook,Lee, Kwang Ho,Choi, Sang Hyun,Kang, Hyeog,Chun, Boe Gwun The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.5

We studied the effects of ASC-17D rat Sertoli cell-conditioned media (rSCCM) on the proliferation of the DU145 prostate cancer cells. rSCCM was prepared from ASC-17D cells cultured in DMEM/F-12 serum-free media at a nonpermissive temperature of 40℃, which is the condition for the high expression of clusterin. We found that rSCCM could inhibit the proliferation of DU145 cells by arresting the cell cycle in the G1 phase in a dose-dependent manner. This growth arresting activity was abolished by boiling rSCCM for 5 min. The G1 growth-inhibiting activity of rSCCM was also detected in other prostate-originated cancer cells examined (ie., LNCaP and PC-3) but not in other cells (ASC-17D, HepG2, SK-N-SH, and NIH3T3). Western blot analysis of partially purified growth inhibiting fractions with the clusterin antibody showed that the cytostatic factor in rSCCM was not clusterin. This cytostatic factor was semipurified by DEAE-Sepharose, ammonium sulfate precipitation, and Phenyl-Sepharose column chromatography, and was estimated to have a molecular weight of 88 kDa by Sephacryl S-300 gel filtration.

Microfluidic generation of Prussian blue-laden magnetic micro-adsorbents for cesium removal

Kang, Sung-Min,Rethinasabapathy, Muruganantham,Hwang, Seung Kuy,Lee, Go-Woon,Jang, Sung-Chan,Kwak, Cheol Hwan,Choe, Sang-Rak,Huh, Yun Suk Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.341 No.-

<P><B>Abstract</B></P> <P>Here, we designed and synthesized a recoverable multifunctional adsorbent using a microfluidic reaction system and evaluated the removal performance of the smart adsorbent toward radioactive cesium as a model sample. Prussian blue-laden magnetic micro-adsorbents (PB-MNPs-MAs) with uniform morphology and monodispersity were generated via two-step sequential procedures using a glass capillary microfluidic system, followed by chemical co-precipitation with a high production rate. The cesium removal efficacy of the PB-MNPs-MAs was analyzed based on Langmuir and Freundlich isotherms by controlling adsorption parameters such as adsorbent size, initial cesium concentration, and contact time. The adsorption isotherm of the PB-MNPs-MAs was better fitted to the Langmuir model with a maximum cesium adsorption capacity of 58.73 mg g<SUP>−1</SUP>, which was 40% higher than that of macro-adsorbents in a dynamic magnetic field. This result can be attributed to their large specific area, which increased the kinetic rate of cesium adsorption and achieved saturation within 20 min. Additionally, the PB-MNPs-MAs were recovered from wastewater within 5 s under a static magnetic field, indicating their great potential for magnetic actuation. We believe that our PB-MNPs-MAs can encapsulate nano-functional adsorbents and prevent actuation, making them promising for environmental remediation and especially for removal of radionuclides.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PB-MNPs-MAs were generated in microfluidic device using chemical co-precipitation. </LI> <LI> The prepared PB-MNPs-MAs are monodispersed with uniform morphology. </LI> <LI> PB-MNPs-MAs exhibited high Cs adsorption capacity (58.73 mg g<SUP>−1</SUP>). </LI> <LI> 100% recovery of PB-MNPs-MAs is possible under static magnetic field after Cs adsorption. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Conditioned Media of ASC-17D Sertoli Cells Induce G1-Growth Arrest of DU145 Human Prostate Cancer Cells

Kang, Hyeog,Kang, Sang-Wook,Choi, Sang-Hyun,Lee, Kwang-Ho,Chun, Boe-Gwun,Min, Bon-Hong Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.5

We studied the effects of ASC-17D rat Sertoli cell-conditioned media (rSCCM) on the proliferation of the DU145 prostate cancer cells. rSCCM was prepared from ASC-17D cells cultured in DMEM/F-12 serum-free media at a nonpermissive temperature of $40^{\circ}C$, which is the condition for the high expression of c1usterin. We found that rSCCM could inhibit the proliferation of DU145 cells by arresting the cell cycle in the G1 phase in a dose-dependent manner. This growth arresting activity was abolished by boiling rSCCM for 5 min. The G1 growth-inhibiting activity of rSCCM was also detected in other prostate-originated cancer cells examined (i.e., LNCaP and PC-3) but not in other cells (ASC-17D, HepG2, SK-N-SH, and NIH3T3). Western blot analysis of partially purified growth inhibiting fractions with the clusterin antibody showed that the cytostatic factor in rSCCM was not c1usterin. This cytostatic factor was semi purified by DEAE-Sepharose, ammonium sulfate precipitation, and Phenyl-Sepharose column chromatography, and was estimated to have a molecular weight of 88 kDa by Sephacryl S-300 gel filtration.

Preparation and Properties of Polyurethane Modified with Polyorganosiloxane Sealant

Kang, Doo Whan,Kim, Young Min,Lee, Sang Moon,Han, Mi Sun,Kang, Ho Jong 한국공업화학회 2001 Journal of Industrial and Engineering Chemistry Vol.7 No.4

α, ω-Trimethoxysilyl polyurethane modified with polyorganosiloxane (TUMPS) sealant was prepared by adding additives, such as plasticizer, crosslinking agent, and viscosity increasing agent, to α, ω-trimethoxysilyl polyurethane modified with polyorganosiloxane hybrid elastomer at room temperature under nitrogen atmosphere. The TUMPS hybrid elastomer was crosslinked by the sol-gel reaction of methoxy group of TUMPS with hydroxyl group in the building stone and moisture of the air. It was found that TUMPS sealant exhibited more stable rheological and sealant properties than those of TUMPS hybrid elastomer. The shrinkage rate of TUMPS sealant was 5.82-6.0%, and skin over time was about 40 min. Alkaline resistance showed good characteristics for 15 days, slump was about 4.0-4.3㎜, and oil content, as the diameter soaked oil from wetted filter paper was 1-2㎜ after 7 days.

친환경 세척제의 처리 방법을 달리하여 착즙한 당근 주스의 미생물 안전성 및 품질

임상욱(Sang-Wook Lim),최다정(Da-Jeong Choe),강민정(Min-Jung Kang),김종현(Jong-Hyun Kim),김묘정(Myo-Jeong Kim),김민주(Min-Ju Kim) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.10

본 연구에서는 당근의 세척에 사용되는 친환경 세척제의 초기 미생물 제어 효과를 향상시키기 위해 탄산수소나트륨과 구연산의 단독 및 병합 처리 조건을 설정하였으며 저장 중 당근 주스의 미생물 수 및 품질 특성 변화를 분석하였다. 친환경 세척제의 단독 처리 방법으로는 0.5, 1, 2% 탄산나트륨수와 0.2, 0.5, 1% 구연산수를 적용하였고, 살균 효과를 비교하기 위해 무처리구, 수돗물 처리구, 50 ppm 차아염소산나트륨 처리구를 사용하였다. 당근의 세척을 위한 병합처리 방법으로는 단독 처리에서 우수한 살균 효과를 나타낸 1% 탄산수소나트륨수와 1% 구연산수를 이용하였고, 1% 탄산수소나트륨수 처리 후 1% 구연산수로 처리하는 단계적 병합 처리구가 가장 우수한 살균 효과를 나타내었다. 1% 탄산수소나트륨수와 1% 구연산수의 단계적 병합 처리구는 무처리구에 비해 일반 세균수 1.87 log CFU/mL, 대장균군 1.56 log CFU/mL를 감소시켰고, 50 ppm 차아염소산수에 비해서도 일반 세균수 1.07 log CFU/mL, 대장균군 0.22 log CFU/mL의 미생물 감균효과를 나타냈다. 1% 탄산수소나트륨수 처리 후 1% 구연산수의 단계적 병합 처리구를 4°C에서 7일 동안 저장한 결과 수돗물, 50 ppm 차아염소산수나 혼합 병합 처리구, 1% 구연산수 처리 후 1% 탄산수소나트륨 처리구에 비해 미생물 제어 효과가 확실하게 나타났지만, pH, 당도, 산도, 색도에는 유의적인 차이가 나타나지 않았다. 본 연구 결과 1% 탄산수소나트륨과 1% 구연산의 단계적 병합 처리는 당근 세척 시 초기 미생물 제어와 품질 유지에 있어 효과적인 살균처리 방법으로 비가열 당근 주스의 유통기간 동안 미생물 안전기준에 부합하며 주스의 품질을 유지할 수 있는 바람직한 세척방법으로 판단된다. The aim of this study was to evaluate the microbial inhibitory activity and physicochemical quality of fresh carrot juice prepared with different environmentally-friendly washing methods during low temperature storage. Individual and combined treatments with sodium bicarbonate (baking soda, NaHCO₃) and citric acid were applied to carrots for 10 min. Tap water and 50 ppm of sodium hypochlorite (NaOCl) were used as the control. Combined treatment of 1% NaHCO₃ and 1% citric acid significantly reduced total aerobic counts and coliforms. In addition, combined treatment of 1% NaHCO₃ and 1% citric acid inhibited microbial growth for 7 days at 4°C and 10°C in a shelf-life study. There were no significant differences among the sanitizers in terms of °Brix, acidity, pH, and color. Changes in physicochemical quality were not significantly different by sanitizer but were affected by storage temperature. These results indicate that washing with combined treatment of 1% NaHCO₃ and 1% citric acid is an effective method to inhibit the microbial population and maintain physicochemical quality. Therefore, combined treatment of 1% NaHCO₃ and 1% citric acid can be effectively used to sanitize and prepare carrot juice without affecting other properties.

보문 : 항고혈압성 안지오텐신 전환효소 저해제를 생산하는 새로운 효모의 선별 및 저해물질 최적 생산조건

강민구 ( Min Gu Kang ),김하근 ( Ha Kun Kim ),이성훈 ( Sung Hun Yi ),임성일 ( Sung Il Lim ),이종수 ( Jong Soo Lee ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3

Forty eight strains of yeast were cultured in potato dextorse(PD) broth at 30˚C for 24 hr and centrifuged with 12,000 rpm for 20 min. After concentrated the cultures, antihypertensive angiotensin I-converting enzyme(ACE) inhibitory activities of its concentrates were investigated. Among them, the concentrates from Saccharomyces cerevisiae Y183-3 showed the highest ACE inhibitory activity of 71.8%. The ACE inhibitor from Saccharomyces cerevisiae Y183-3 was maximally produced when Saccharomyces cerevisiae Y183-3 cultured in PD broth at 30˚C for 36 hr.

레미콘 슬러지를 이용한 불소제거

강민구(Min Koo Kang),신관우(Gwan Woo Shin),이상일(Sang Ill Lee) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.11

본 연구의 목적은 칼슘염을 함유한 레미콘 슬러지를 이용하여 불소함유폐수를 물에 난용성인 CaF₂ 화합물 형태로 침전시켜 처리하는데 있어 pH, 레미콘슬러지 주입량, Seed물질 주입량, 교반속도, 교반시간의 최적 조건을 도출하고자 하는데 있다. 실험결과 레미콘 슬러지를 이용한 대상폐수의 CaF₂ 침전 반응에서 함수율 및 불소의 제거효율 등을 고려할 경우 최적의 pH 6, 레미콘 슬러지 주입량은 10 g/L, Seed 물질 주입량은 2 g/L, 교반속도은 100 rpm, 교반시간은 60 min으로 관찰되었다. 이 때 Seed 물질의 주입은 불소제거효율에는 영향이 없는 것으로 나타났지만 침전물의 형성이 되는 반응을 촉진시키고 플럭형성을 원활하게 하여 고액 분리가 잘 되어 함수율이 낮아지는 결과가 나타났다. The purpose of this study was performed to investigate the optimum conditions of pH, concrete sludge, seed dosage, mixing intensity, operation time in treating fluoride-containing wastewater as CaF₂ using the ready-mixed concrete sludge. Considering fluoride removal, water content, that pH 6, concrete sludge dosage of 10 g/L, Seed dosage (CaF₂) of 2 g/L, mixing intensity of 100 rpm and operation time of 60 min were found to be optimum. Correspondingly, removal of fluoride and water content was about 85% and 64%, respectively. Increase in amount of seed dosage did not affect fluoride removal efficiency. but the result that the water content is decreased was shown up in occuring the solid-liquid separation well.

Analysis of Lignans in Acanthopanax sessiliflorus Fruits and Their Fermented Wine by HPLC

Hye-Min Kim,Ju-Sun Kim,Seon-Haeng Cho,Sam-Sik Kang,Dae-Sung Cheoi,Sang-Hyun Lee 한국약용작물학회 2006 한국약용작물학회지 Vol.14 No.5

High performance liquid chromatography (HPLC) was used for the determination of lignans, eleutherosides B and E, in Acanthopanax sessiliflorus fruits and their fermented wine. The lignans were quantified by a reversed-phase system using a gradient of H2O and acetonitrile as a mobile phase within 20 min. The analysis was successfully carried out within 20 min. The contents of eleutherosides Band E as main active principles of Acanthopanax species were measured in A. sessiliflorus fruits (1.15 and 8.49 μg/mg, respectively), their fermented wine (0.45 and 1.33 μg/mg, respectively) and wine residues (no detection).