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Liwei Ren,Honghua Jia,Min Yu,Wenzhong Shen,Hua Zhou,Ping Wei 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5
Candida rugosa lipase (CRL) is one of the most widely used lipases. To enhance the catalytic abilities of CRL in both aqueous and non-aqueous phases, hollow silica microspheres (HSMSs) with a pore size of 18.07 nm were used as an immobilization support, and aldehydecontaining dextrans were employed to further cross-link the adsorbed CRL. In the experimental ranges examined,the loading amount of lipase linearly increased to 171 ±3.4 mgprotein/gsupport with the CRL concentration and all the adsorption equilibriums were reached within 30 min. After simple cross-linking, the tolerance to pH 4.0 ~ 8.0 as well as the thermal stability of immobilized CRL at 40 ~ 80oC were both substantially increased, and 82 ± 2.1% activity remaining after the sixth reuse. The immobilized CRL was successfully applied to the resolution of racemic ibuprofen in non-aqueous phase. The initial reaction rate increased by 1.4- and 3.6-fold compared with the rates of adsorbed and native lipases, respectively. Furthermore, the R-ibuprofen was obtained at ee > 93%, and the enantiomeric ratio reached E > 140 at the conversion of 50 ± 1.5% within 48 h.
Prediction of Failure Mode in Hole Clinching of Al Alloy and Advanced High-Strength Steel
Shen, Guo,Lee, Chan Joo,Lee, Jeong Min,Kang, Gil Seok,Park, Joon Hong,Kim, Byung Min,Ko, Dae Cheol Trans Tech Publications, Ltd. 2016 Key Engineering Materials Vol.716 No.-
<P>Common joining methods used in automotive industry are welding, adhesive bonding, friction stir welding, mechanical fastening, self-piercing rivets, mechanical clinching and so on, for multi-material designed automotive bodies. Among different joining methods, mechanical clinching which achieves geometrical interlocking by plastic deformation has several advantages such as no need of additional joining elements and fast joining. But mechanical clinching is difficult to join a ductile material with a high-strength or low ductility material. Therefore the hole clinching as a new mechanical clinching process has been proposed to join these material combinations. In the hole clinching process, as punch force is applied to a upper sheet (a ductile material), it is indented into hole of lower sheet (low ductile material) on die and then interlocked by plastic deformation. It is very important for a successful design of hole clinching to predict the failure mode such as neck fracture and button separation and the strength of hole-clinched joint. For this an analytical approach was carried out for the hole clinching process of Al6061 and DP980. Tool geometry used in hole clinching was designed by the predicted failure mode. Preliminary finite element simulation was performed to validate the geometrical interlocking and joinability. The predicted failure mode and strength were verified by the results of cross tension test.</P>
Min Shen,H. Dean Hosgood,Luoping Zhang,이경무,Roel Vermeulen,Guilan Li,Songnian Yin,Nathaniel Rothman,Stephen Chanock,Martyn T. Smith,Qing Lan 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.6
Benzene, a recognized hematotoxicant and carcinogen,can damage the human immune system. We studied the association between single nucleotide polymorphisms (SNPs) in genes involved in innate immunity and benzene hematotoxicity in a cross-sectional study of workers exposed to benzene (250 workers and 140 controls). A total of 1,236 tag SNPs in 149gene regions of six pathways were included in the analysis. Six gene regions were significant for their association with white blood cell (WBC) counts (MBP,VCAM1, ALOX5, MPO, RAC2, and CRP) based on gene-region (P < 0.05) and SNP analyses (FDR <0.05). VCAM1 rs3176867, ALOX5 rs7099684, and MPO rs2071409 were the three most significant SNPs. They showed similar effects on WBC subtypes, especially granulocytes, lymphocytes, and monocytes. A 3-SNP block in ALOXE3 (rs7215658, rs9892383, and rs3027208) showed a global association (omnibus P =0.0008) with WBCs even though the three SNPs were not significant individually. Our study suggests that polymorphisms in innate immunity genes may play a role in benzene-induced hematotoxicity; however, independent replication is necessary.
( Min Shen ),( Xin Ju ),( Xinqi Xu ),( Xuemei Yao ),( Liangzhi Li ),( Jiajia Chen ),( Cuiying Hu ),( Jiaolong Fu ),( Lishi Yan ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.7
In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of L-rhamnose to L-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions (50oC, pH 8.0, and 1 mM Ca<sup>2+</sup>) were obtained to maximize the potential of OsRpiB in preparing L-rhamnulose. The catalytic properties of OsRpiB, including K<sub>m</sub>, K<sub>cat</sub>, and catalytic efficiency (K<sub>cat</sub>/K<sub>m</sub>), were determined as 43.47 mM, 129.4 sec<sup>-1</sup>, and 2.98 mM/sec. The highest conversion rate of L-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of L-rhamnose to L-rhamnulose by OsRpiB biocatalysis.
Adaptive Nonsingular Fast Terminal Sliding Mode Guidance Law with Impact Angle Constraints
Shen-Min Song,Junhong Song,Huibo Zhou 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.1
This paper considers the terminal guidance problem of missiles intercepting maneuvering targets withimpact angle constraints. Based on an advanced nonsingular fast terminal sliding mode control scheme and adaptivecontrol, an adaptive nonsingular fast terminal sliding mode guidance law is proposed in the presence of the targetacceleration as an unknown bounded external disturbance. In the design procedure, an adaptive law is presentedto estimate the unknown upper bound of the external disturbance. Theoretical analysis shows that the proposedguidance law can guarantee the finite-time convergence in both the reaching phase and the sliding phase by applyinga Lyapunov-based approach. Numerical simulations are presented to demonstrate the effectiveness of the proposedguidance law. Although the proposed guidance law is developed for the constant speed missiles, by the extensivenumerical simulations with a realistic missile model, the performance is shown to be equally good for the varyingspeed missiles.
A Literature Survey on High-Dimensional Sparse Principal Component Analysis
Shen Ning-min,Li Jing 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.6
Principal Component Analysis (PCA) is a classical method for dimensionality reduction, data pre-processing, compression and visualization of multivariate data for different applications in biology, social science and engineering. The limitation of PCA is lacking of interpretation due to the non-zero loadings and the inconsistence for highdimensional data. Sparse principal component analysis (sparse PCA) is proposed mainly for the challenges of PCA above. For the past decades, many works of the development methods and theoretical analysis for sparse PCA have been presented. The goal of this paper is to give a comprehensive literatures review to recent progress in highdimensional sparse PCA from algorithm and statistical theory. Firstly we give the overview for PCA and sparse PCA. Secondly the algorithms of sparse PCA are categorized into different classes and provide detailed descriptions for typical formulations and methods in each category, and the typical packages of sparse PCA are also given. Considering that statistical analysis in high dimension becomes more involved in sparse PCA, and then the survey of theoretical analysis of sparse PCA is also presented. Finally the future trends as well as challenges are given.
Shen, Min,Zhang, Luoping,Lee, Kyoung-Mu,Vermeulen, Roel,Hosgood, H. Dean,Li, Guilan,Yin, Songnian,Rothman, Nathaniel,Chanock, Stephen,Smith, Martyn T.,Lan, Qing Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.6
Benzene, a recognized hematotoxicant and carcinogen, can damage the human immune system. We studied the association between single nucleotide polymorphisms (SNPs) in genes involved in innate immunity and benzene hematotoxicity in a cross-sectional study of workers exposed to benzene (250 workers and 140 controls). A total of 1,236 tag SNPs in 149 gene regions of six pathways were included in the analysis. Six gene regions were significant for their association with white blood cell (WBC) counts ($MBP$, $VCAM1$, $ALOX5$, $MPO$, $RAC2$, and $CRP$) based on gene-region (P < 0.05) and SNP analyses (FDR <0.05). $VCAM1$ rs3176867, $ALOX5$ rs7099684, and $MPO$ rs2071409 were the three most significant SNPs. They showed similar effects on WBC subtypes, especially granulocytes, lymphocytes, and monocytes. A 3-SNP block in $ALOXE3$ (rs7215658, rs9892383, and rs3027208) showed a global association (omnibus P = 0.0008) with WBCs even though the three SNPs were not significant individually. Our study suggests that polymorphisms in innate immunity genes may play a role in benzene-induced hematotoxicity; however, independent replication is necessary.
Targeting Orthotopic Glioma in Mice with Genetically Engineered Salmonella typhimurium
Wen, Min,Jung, Shin,Moon, Kyung-Sub,Jiang, Shen Nan,Li, Song-Yuan,Min, Jung-Joon The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.55 No.3
Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.