성상교 세포종에서 p53 유전자발현에 대한 면역조직화학적 연구

황금,한용표,조미연,정순이,김헌주 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.3

Nineteen astrocytic neoplasms, such as 9 case of glioblastoma multiforme, 6 case of anaplastic astrocytoma and 4 case of low grade astrocytoma, were analysed in view of the relationship between histopathologic grade, nuclear pleomorhism, grade of mutant p53 gene expression and mean survival time after operation. The histopathologic calssification by Ringertz and immunohistochemical stain for mutant p53 gene with the DO-7 anti-p53 oncoprotein mouse monoclonal antibody were applied, and the results obtained were as follows : 1) Among 19 cases, 18 cases were located in the supratentorium where 13 cases(42%) were located in the left and 17 cases(55%) in the right. 2) The p53 gene expression was detected in 12(63.2%) of the cases, as revealed by positive nuclear staining. All cases of glioblastoma multiforme showed p53 gene expression, compared with two(33.3%) cases of anaplastic astrocytoma and one(25%) case of low grade astrocytoma. The frequency and degree of histopathologic grade(p=0.03) 3) The mean survival time after operation was 29.49±4.08 months in case with p53-negative tumors and 12.93±3.14 months in cases with p53-positive tumors(p<0.05). 4) Both histopathological calssification and p53 gene expression showed a significant influence on servival (p=0.02 and p=0.03, respectively) 5) The relative risk for survival time was the highest in p53 gene expression. In conclusion, p53 gene expression appears to be one of the recommendable prognosticators among astrocytic neoplasms.

Kainic Acid로 처리한 해마박편배양 마우스 간질모델에서 치아이랑 Parvalbumin 면역 반응성 사이신경세포의 형태학적 변화

정희선,신미영,김영훈,이인구,황경태,김명석 대한소아과학회 2002 小兒科 Vol.45 No.12

MCM-41 Supported Chiral Amino Alcohols as Enantioselective Catalysts for the Reduction of Ketones

Whang, Mi Sun,Kwon, Yong Ku,Kim, Geon-Joong 한국공업화학회 2002 Journal of Industrial and Engineering Chemistry Vol.8 No.3

The introduction of functional organic groups onto the mesoporous walls has been performed through the co-condensation of siloxanes and organosiloxane precursors during MCM-formation by solvent evaporation method using C_16TMAC1 surfactant as a template. The organo-functionalized mesoporous materials were used to immobilize the chiral ligands and MCM-41-supported amino alcohols were prepared to use as asymmetric catalysts for reduction of aromatic ketones. The results were compared with those obtained using unsupported amino alcohols. Higher enantioselectivity was achieved on the MCM-41-supported amino alcohols than silica gel-supported ones.

Scopoletin from <em>Cirsium setidens</em> Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells

Mi-Ja Ahn,Sun-Jung Hur,Eun-Hyun Kim,Seung Hoon Lee,Jun Seob Shin,Myo-Kyoung Kim,James A. Uchizono,Wan-Kyunn Whang,Dong-Seok Kim 대한생리학회-대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4

In this study, we isolated scopoletin from <em>Cirsium setidens</em> Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 μM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.

Molecular Analysis of Anthocyanin Biosynthetic Genes and Control of Flower Coloration by Flavonoid 3',5'-Hydroxylase (F3'5'H) in Dendrobium moniliforme

Whang, Sung-Soo,Um, Wan-Sook,Song, In-Ja,Lim, Pyung-Ok,Choi, Kyung,Park, Kwang-Woo,Kang, Kyung-Won,Choi, Mi-Sun,Koo, Ja-Choon 한국식물학회 2011 Journal of Plant Biology Vol.54 No.3

Dendrobium moniliforme is a native species of Korea. The flower of this species is composed of a reproductive column and white perianths including petals, sepals and lip, but the base of the column bears reddish purple pigment spots. Anthocyanins are major pigments that contribute flower color in Dendrobium. Three key anthocyanin biosynthetic genes encoding dihydroflavonol 4-reductase (DFR), chalcone synthase (CHS), and flavonoid 3',5'-hydroxylase (F3'5'H) were isolated and analyzed for their expression patterns in floral organs to understand the molecular mechanism underlying flower color development. Quantitative RT-PCR analysis revealed that F3'5'H transcripts were highly accumulated in the base of the column compared with those of perianths, but the other two genes showed no significant difference among the floral organs. Microprojectile bombardment using the white perianths revealed that the transient expression of F3'5'H gene, but not DFR and CHS genes, was sufficient to produce reddish purple colored pigmentation. These results suggest that the lack of colors in perianths of D. moniliforme is at least due to transcriptional control of F3'5'H. The data presented here may improve our understanding of the mechanisms underlying floral color development in D. moniliforme and contribute to advances in orchid biotechnology.

Metastasis Versus Focal Eosinophilic Infiltration of the Liver in Patients With Extrahepatic Abdominal Cancer: An Evaluation With Gadobenate Dimeglumine-Enhanced Magnetic Resonance Imaging

Park, Mi-Suk,Kim, Myeong-Jin,Lim, Joon Suk,Kim, Seung Hyung,Kim, Hua-Sun,Chung, Yong Eun,Kim, Ki Whang Lippincott Williams Wilkins, Inc. 2009 Journal of computer assisted tomography Vol.33 No.1

PURPOSE:: To determine the performance of gadobenate dimeglumine (Gd-BOPTA)-enhanced magnetic resonance imaging (MRI) to differentiate focal eosinophilic infiltration (FEI) from metastasis. MATERIALS AND METHODS:: Gadobenate dimeglumine-enhanced MRI conducted in 38 patients (lesions of 43 metastases and 33 FEIs) with extrahepatic abdominal carcinoma were reviewed retrospectively. Images were divided into 2 sets. Set 1 was composed of precontrast MRI with dynamic phase images. Set 2 included hepatobiliary phase images in addition to the images of set 1. Two blinded radiologists independently categorized each set of images into 5 grades to differentiate metastases from FEI. The area under the receiver operating characteristic curve (Az) was calculated for each set of images. The observers evaluated the shape, signal intensity (SI), and enhancement pattern of the lesions in consensus. RESULTS:: The Az values for set 2 images (reader 1: 0.976, and reader 2: 0.743) were greater than those of set 1 (0.961 and 0.709), without statistical significance (P = 0.470 and 0.223). Target appearance was seen in 27 of 43 (63%) metastases on hepatobiliary phase imaging, but none on the FEI. Twenty-five (76%) of the 33 FEIs showed intermingled hypo-SI and iso-SI on hepatobiliary phase images with changing size and shape compared to the T2-weighted images, whereas none of the metastases did. CONCLUSION:: Gadobenate dimeglumine-enhanced MRI may assist in the differentiation of FEI from metastasis by highlighting the distinctive features between them, even without hepatobiliary phase imaging.

새우난초류 꽃 기관 내 차등 발현 유전자들의 분석 및 발굴

최미선 ( Mi-sun Choi ),황성수 ( Sung-soo Whang ),구자춘 ( Ja-choon Koo ) 전북대학교 과학교육연구소 2019 과학과 과학교육 논문지 Vol.44 No.2

한라새우난은 한국에 자생하는 Calanthe 속의 난초이다. 한라새우난은 화색, 화형, 향기 등 독특한 꽃 관련 형질로 인해 상업적으로도 중요한 난초로 주목을 받아왔다. 특히 한라새우란의 꽃 기관은 자색의 꽃잎과 꽃받침, 노란색 설판을 가지고 있어 동일한 유전적 배경하에 각 기관의 색을 조절하는 유전자 발굴에 이상적인 샘플이라 할 수 있겠다. 따라서 꽃 발달이나 화색 조절에 관련된 유전자를 differential display기법을 이용하여 분석하였다. 총 42개의 유전자가 차등발현되는 것을 발굴하였고 이중 31개의 유전자가 최종적으로 각 기관마다 차등 발현되는 것을 확인 하였다. 이 유전자들을 분석한 결과 calmodulin, annextin과 GTPase SAR1 등 신호전달에 관련된 것으로 보이는 유전자들과 안토시아닌 생합성과 관련 있는 것으로 알려져 화색과 관련될 것으로 예상되는 Flavonoid 3'-hydroxylase, MYB1을 발견하였고, 꽃잎발달 과정에 관련되었다고 알려진 MYR-related protein, 식물의 성숙과 노화에 관련된 호르몬인 에틸렌의 생합성 초기 단계에 관여하는 S-adenosylmethionine synthase와 대표적인 식물의 생장호르몬으로 알려진 옥신 family중 하나인 IAA1, 그리고 꽃의 향기와 관련될 것으로 예상되는 4-coumarate-CoA ligase등의 유전자를 확인하였다. 본 연구는 우리나라 자생종인 한라새우난에 대한 첫 분자적 접근의 연구라는 점과 더 나아가 각 조직별로 비교하여 차등발현되는 유전자를 선별하였다는 데 그 의미를 갖는다. 또한 같은 꽃 기관을 사용하여서 종간 유전자 차이를 배제 할 수 있어, 한라새우난의 화형과 화색에 관한 유전자 기능연구에 큰 도움이 될 것으로 예상된다. Calanthe bicolor is a species of the genus Calanthe native to Korea. C. bicolor flower contains valuable floral traits including unique flower colors, morphologies and scents, thus it has been regarded as one of the most important commercial orchid in floricultural industry. C. bicolor is a natural hybrid orchid between C. bicolor Lindl and C. striata Ohwi, which is generally found in the southern island of Korea. Since flower of C. bicolor bears bicolored perianth organs including reddish brown sepals and petals and yellow lip, the each floral organ was dissected and subjected to gene expression analysis by differential display RT-PCR (DDRT-PCR) to understand the molecular mechanisms underlying floral development associated with floral color and morphology. A total of 42 gene were amplified by DD-RTPCR. Of these, 31 of the 42 genes were found to be differentially expressed in each floral tissue. Moreover, the sequence analysis indicated that the expression patterns of a subset of genes encoding proteins presumably involved in anthocyanin biosynthetic genes, signal transduction, metabolic pathways and hormonal signaling were differed among floral organs. This suggests that the isolated genes might have unique roles in floral development of each floral tissues. The data presented here may improve our understanding of the mechanisms underlying floral development and contribute to improve orchid biotechnology as gene sources.

고춧가루와 Capsaicin 이 위배출에 미치는 영향

윤선,이상인,박효진,이송미,박인서,김정호,나상규,김기황,정대호 대한소화기학회 1999 대한소화기학회지 Vol.33 No.4

Background/Aims: Capsaicin stimulates the release of several neuropeptides and has diverse effects on gastrointestinal function. We investigated the effect of intragastric red pepper or capsaicin on the gastric emptying in human. Methods: Fourteen healthy male volunteers were recruited. Gastric emptying was assessed by radio-opaque markers (ROMs) method and plasma acetaminophen (AAP) levels. Results: The clearance of ROMs at 2 hours and 3 hours was 10.6±15.9 and 73.1 ±34.6% after administration of placebo, 17.6±26.0 and 78.7±40.2% after administration of red pepper 3 g 27.8 ±34.0 and 73.2 ±31.9% after administration of red pepper 6 g, 2.1 ±5.1 and 15.5 ±20.7% afte administration of capsaicin 17.3 mg. Capsaicin significantly delayed the gastric emptying of ROMs The serum AAP concentrations were measured at 30, 45, 60, 75 and 90 min after administration o placebo (4.09 ±3.45, 8.09 ±4.13, 13.55 ±4.90, 15.50 ±3.44 and 13.0 ±7.53 ㎍/ml), red pepper 3 g (5.63 ±4.84, 8.88 ±4.76, 14.25 ±5.01, 15.0 ±6.57 ㎍/ml), red pepper 6 g (7.0 ± 7.19, 8.09 ±5.63, 12.09 ±6.04, 13.73 ±4.65 and 14.28 ±3.77 ㎍/ml), capsaicin 17.3 mg (4.50 ±2.88, 7.17 ±3.19, 11.50 ±4.76, 11.17 ±3.71 and 13.33 ±3.72 ㎍/ml). Intragastric red pepper or capsaicin made no significant difference of serum acetaminophen level from placebo. Conclusions: Intragastric administration of capsaicin delayed gastric emptying of indigestible solid meal, whereas red pepper did not. The gastric emptying of liquid meal was affected by neither capsaicin nor red pepper.

Retinoic Acid Enhances Drug-Induced Cell Death in Anticancer Drug-Resistant Cell Lines

Young Mi Whang,Yeul Hong Kim,Sang Won Shin,Byung Soo Kim,Jun Suk Kim,Young Do Yoo,Sun-Hee Park 대한암학회 2002 Cancer Research and Treatment Vol.34 No.3

Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells

Ahn, Mi-Ja,Hur, Sun-Jung,Kim, Eun-Hyun,Lee, Seung Hoon,Shin, Jun Seob,Kim, Myo-Kyoung,Uchizono, James A.,Whang, Wan-Kyunn,Kim, Dong-Seok The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4

In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than $50{\mu}M$ and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.