심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

학교 통일교육지침에 입각한 유치원의 통일교육 구현 방향 모색

윤기영,이미숙 서원대학교 교육연구소 1995 敎育發展 Vol.1995 No.14

The purpose of this study was to investigate how the kindergarten education for reunification in Korea should be, based on the 「Guides for School Teacher」published by Education Ministry. Necessity, appropriateness, and possibility of this education were considered. The results of this study were as follows; Education for reunification in Korea must be done in kindergarten as well as inordinary school because reunification of Korea is essential for us. Also many concepts and characters related with this education are formed and developed in early childhood. And it is one of reasons that early childhood deucation is very important as well as known. So, for successful this education systematic programs should be developed, and education about reunification should be reinforced for kindergarten teacher. Howerer, for the time being, since new programs are not yet developed it is desirable that the current programs such as 「human Education」, 「Democratic Citizenship Education」,「Education for peace」 be used instead to achieve this goal.

치과진료실에서의 감염방지 대책에 관한 조사

윤미숙,홍연표,정연강 중앙대학교 의과대학 간호학과 간호과학연구소 1997 중앙간호논문집 Vol.1 No.2

To investigate on infection control in some dental clinics in korea. This study was performed on 300 hygienists who are registered on the association of korea dental hygienist and work at dental clinics from November to December, 1995. Each subjects completed the questionnaire which compased of place of employment of dental hygienist, location of clinics, number of outpatients, educational experience about infection control of dental hygienist on infection control and age of dentists as independent varianbles and use of sterilization methods and protection devices as dependent variables. Data Was expressed as percentage and anlayzed as chi-square method using SPSS/PC + 4.0. There was a significant higher performance rate in use of sterilization methods and protection devices in dental clinics of general hospital than private and public dental clinics. There was no difference in use of sterilization methods and protection devices according to location, number of outpatients of dental clinics and age of dentists. In case of dental hygienists who getting educational experience about infection control, the rate of use of sterilization methods and protective devices was higher than no educational experience. This results suggested that the education about infection control and the follow-up study to investigate he causes of low performance in use sterilizsation methods and protective devices in dental clinics was needed.

한국의 외식문화 변천에 관한 연구

尹淑賢,李媄貞 호남대학교 산업기술연구소 2003 산업기술연구논문집 Vol.11 No.-

서울지역 주부들의 혼례음식에 대한 인지도 조사

윤숙자,이미영 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.5

본 연구는 서울 지역의 주부를 대상으로 하여 혼례음식(폐백과 이바지 음식)의 인식상황과 실태를 조사함으로써 혼례음식 발전의 기초자료를 제공하고자 얻은 결과를 요약하면 다음과 같다. 첫째, 폐백음식의 인식에 대해서는 "결혼 후 시댁에 인사를 드리는 것"이라는 응답이 55.2%, "옛날부터 내려오는 하나의 풍습"이 35.8%로 나타나 상당히 정확하게 알고 잇는 것으로 나타났다. 폐백음식의 중요도 인식수준은 전체 응답자의 10.2%가 "매우 중요하다"라고 응답하였으며, "중요하다" 48.5%, "중요하지 않다"는 11.9%로 나타나 폐백음식의 중요성에 대하여 공감하는 비율이 높게 나타났다. 특히 주부의 연령별로 20대 집단에서 30대 이상의 집단에 비해 "중요하지 않다"는 응답율이 높게 나타났다.(p<0.05) 둘째, 폐백음식 준비방법으로는 "가정에서 직접 마련"한다와 "시장이나 떡집에서 마련"하겠다는 응답이 비슷한 비율을 보였으며, 연령별·교육수준별(p<0.05)로 유의한 차이를 보였다. 폐백음식 장만시 드는 비용으로는 "30~50만원"이라고 응답한 비율이 50%로 가장 높게 나타났고, "10~30만원"29%>"50~100만원"18% 순으로 나타났다. 셋째, 선호하는 폐백음식으로는 "밤·대구고임"을 준비한다는 의견이 77.5%로 가장 높게 나타났으며, 그 다음으로 "떡류">"육포">"술">"한과" 등의 순으로 나타났다. 넷째, 이바지음식에 대한 의미로는 48.2%가 "신부측에서 마련한 음식을 시댁에 보내는 것"이라고 응답하였으며, 이바지 음식의 필요성에 대해서는 "반드시 준비해야 한다"라는 의견이 15.5%, "형편에 맞추어 준비한다"는 78.7%, "준비할 필요가 없다"5.8%로 나타나 전반적으로 이바지 음식의 필요성에 대해서 공감대를 형성하고 잇는 것으로 나타났다. 또한 취업주부가 "필요하다"라는 응답이 높게 나타났고, 종교적으로도 불교를 믿는 주부보다 기독교를 믿는 주부들에게서 유의적인 차이를 보였다.(p<0.01) 다섯 번째, 혼례음식의 개선점으로 메뉴의 간소화>가격을 저렴하게 하는 문제>폐백음식 선택의 다양화 등으로 나타났다. 연령별로 40대 주부집단에서 "메뉴의 간소화" 응답율이 높게 나타났다.(p<0.01) 이상의 같은 연구 결과로 보아 우리나라의 모범이 되는 혼례음식의 계승을 위해서 꾸준한 노력과 관심이 필요하다고 사료된다. 과거와 다른 신식혼례를 치르면서도 폐백을 드리고 이바지 음식 보내는 풍습은 계속 이어지고 있다. 이러한 혼례 풍습은 계속 이어질 것이기에 혼례음식이 가지고 잇는 기복과 주술적인 의미를 되살리면서 저렴한 가격으로 누구나 손쉽게 장만할 수 잇는 간편한 방법이 모색되어 전통을 살리면서 모범이 되는 혼례음식문화를 계승 발전시켜 나가야 하겠다. This study was carried out to survey the degree of the recognition and necessity of wedding food to include Pha`ebak and Ibaji foods prepared by bride's parents and sent to the bridgroom's. The participants of the survey were 293 housewives of 20 years old or more residing in Seoul area. The questionnaires used in this research consisted of 10 questions. The findings could be summarized as follows: 1. As to the degree of the recognition of Pha`ebak food, 55.2 percent of the participants perceive it as 'the food sent to the bridegroomvs house to be used when the newly-weds greet groom's relatives, whereas 35.8 percent regarded the practice as "a traditional custom from the ancient times." which is, in fact, correct and exact. As to its importance, 10.2 percent regarded Pha`ebak food very important, while 48.5 percent, "important," and 11.9 percent, "not important." 2. As for the items for Pha`ebak dishes, 77.5 percent answered their preferences for "jujube prop-nut," followed by "ricecake," "liquor" and "Korean cookies," in descending order. 3. As for the meaning of Ibaji dishes, 48.2% understand them as "a set of dishes prepared by the bride's parents to be sent to the bridegroom's"; and as for their willingness or necessity of sending the Ibaji dishes, 15.5 percent indicated that they might prepare them, whereas 78.7 percent said that they would prepare them if the situation required. On the other hand 5.8 percent did not feel the necessity. The overall result is that most females surveyed recognized the two types of food important and were inclined to prepare them when the occasion demanded.

정상 성인에서 Haloperidol이 수면구조와 정신생리적 기능에 미치는 영향

윤미숙,신석철,왕성근 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

To investigate the effects of haolperidol on sleep structure and psychophysiologic function in normal adults, author applied polysomnography for sleep structure, reaction time for attention and visual analogue scales for daytime fatigue, mood and sleepiness to normal volunteers. The subjects were 8 healthy young adults, aged from 20 to 24 years old. The results were summarized as follows. 1. Haloperidol has the effects of decreased total sleep time, delayed sleep latency, increased slow wave sleep(SWS) and REM sleep, and decreased waking after sleep onset (WASO) and stage 1 sleep. 2. Delayed sleep latency continued until first recovery night, and increased REM sleep contiued nutil second recovery night. 3. In the night of haloperidol administration, decreased total sleep time correlated with increased slow wave sleep(SWS) and REM sleep, and increased daytime sleepiness, decreased waking after sleep onset(WASO) correlated with increased slow wave sleep(SWS) and REM sleep. Increased REM sleep correlated with decreased daytime sleepiness, and daytime fatigue correlated with mood. 4. In the first recovery night, decreased total sleep time correlted with increased REM sleep and continued until second recovery night, and daytime sleepiness correlated with fatigue, sleepiness and reaction time.

유산소운동이 고혈압환자의 혈청지질 및 호르몬에 미치는 영향

윤미숙 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

The purpose of this study was to investigate the changes of body composition, circulorespiratory functions, serum lipids and hormones related controlling blood pressure after 16weeks aerobic exrcise. Subjects were composed of twenty old female hypertensive patients. V˙O_2max was determined for each subjects by administering a treadmill exercise test. The exercise intensities are decided by regression equations between %HRmax and speed(m/min). Subjects were trained during 16weeks(50%HRmax, 4 frequencies/week, 1 hour). Paired t-test was used to evaluate the difference of the pre and post training. The 0.05 level of significance was as critical level for this study. The result of this study are as follows: 1. Weight and %fat were decreased after 16weeks aerobic training. 2. Circulorespiratory function was improved because of increasing in V˙O_2max, V˙O_2max/kg after 16weeks aerobic training. 3. As Total cholesterol, Low density lipoprotein cholesterol, Triglyceride and Apolipoprotein B were density and High density lipoprotein cholesterol was increased after 16weeks aerobic training, Blood pressure was decreased. 4. As plasma norepinephrine, renin, angiotension Ⅱ, Aldosterone and α-ANP wee decreased, so blood pressure was decreased because of decreasing total peripheral resistance In conclusion, Aerobic training was shown to positive influence on decreasing blood pressure because of improving plasma lipids and decreasing peripheral vascular resistance in hypertensive patients.

중량물 운반시 잡기유형과 부하단계에 따른 생리학적 연구

윤양진,이훈식,윤길환,정미라,이재규,이중숙,서국웅 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to examine physiological changes of the body according to various weight loads and the holding during carrying object. The experiments were conducted with utilizing physiological analyses for five male and five female adults as subjects. The results were as followed. 1. Any significant difference was not shown in heart rate, ventilation and amount of oxygen uptake of male subjects in the case of no load and 5kg. And also no significant difference was shown in expiratory exchange ratio of male subjects of no load, 5kg and 10kg, but in other measure items and other stages of weight was shown. 2. Significant differences were shown in heart rate, ventilation, defluxion of carbon dioxide, oxygen uptake per body weights, respiratory exchange rate, respiration rate and all other measure items of female subjects in all stages of load. 3. In the MMH job, there was no influence on male subjects up to 5kg loads, but there was an significant difference in male subjects in load walking from 5kg. It shows that even in 5kg weight MMH work, female subject's expiratory function was influenced. 4. 10kg load was the limit weight of female subjects and 15kg was of male subjects when they walked on treadmills with 4km/h velocity for 3 minutes, holding loads in the right and vertical types.

노루궁뎅이 버섯 분말을 첨가한 설기떡의 품질특성

윤숙자,이미영 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.6

This study was carried out to investigate the characteristics of Hericium erinaceus sulgidduk. The moisture content of Hericium erinaceus powder sulgidduk showed no significant difference between the added Hericium erinaceus powder and control groups. The L value decreased as the Hericium erinaceus powder concentration increased. The 'a' and 'b' color values were increased significantly when the amount of Hericium erinaceus powder was increased. The hardness, cohesiveness, chewiness and gumminess decreased with increasing Hericium erinaceus powder addition. From the sensory evaluation, Hericium erinaceus sulgidduk with the additions of 3 and 6% Hericium erinaceus powder were the most preferred with regard tochewiness, softness and overall acceptability. These results indicated that Hericium erinaceus sulgidduk with the addition of 3 and 6% Hericium erinaceus powder have the best quality.