Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

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청각장애초등학생을 위한 음악교육프로그램 개발 연구

이드보라ㆍ남선화ㆍ정미라(Debora LeeㆍNam Sun WhaㆍJeong Mi ra) 한국특수교육학회 2016 특수교육학연구 Vol.50 No.4

3′‐Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

Kang, Li‐,Jung,Kwon, Eun‐,Soo,Lee, Kwang Min,Cho, Chanmi,Lee, Jae‐,In,Ryu, Young Bae,Youm, Tae Hyun,Jeon, Jimin,Cho, Mi Ra,Jeong, Seon‐,Yong,Lee, Sang‐,Rae,Kim, Wook,Yang John Wiley and Sons Inc. 2018 British journal of pharmacology Vol.175 No.23

<P><B>Background and Purpose</B></P><P>3′‐Sialyllactose (3′‐SL) is a safe compound that is present in high levels in human milk. Although it has anti‐inflammatory properties and supports immune homeostasis, its effect on collagen‐induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3′‐SL on the progression of rheumatoid arthritis (RA) in <I>in vitro</I> and <I>in vivo</I> models.</P><P><B>Experimental Approach</B></P><P>The anti‐arthritic effect of 3′‐SL was analysed with fibroblast‐like synoviocytes <I>in vitro</I> and an <I>in vivo</I> mouse model of CIA. RT‐PCR, Western blotting and ELISA were performed to evaluate its effects <I>in vitro</I>. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin‐O and haematoxylin staining.</P><P><B>Key Results</B></P><P>3′‐SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3′‐SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro‐inflammatory cytokines, https://en.wikipedia.org/wiki/Matrix_metalloproteinases and osteoclastogenesis <I>via</I> NF‐κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF‐κB signalling pathway, was totally blocked by 3′‐SL in the RA models.</P><P><B>Conclusions and Implications</B></P><P>3′‐SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated <I>via</I> blockade of the NF‐κB signalling pathway. Therefore, 3′‐SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA.</P>

초등학생용 영어 학습 CD-ROM TITEL유형과 학습자 변인에 따른 학습 효과 비교연구

이동한,김은주,조미라 부산교육대학교 교육대학원 1999 논문집 Vol.1 No.-

The purpose of this study is to investigate the effectiveness of CD-Rom for speaking and listening study in elementary English class. This study also examines various types of CD-Rom titles used in the elementary English education and their effectiveness on learning English speaking and listening for the students who have different variables. The result shows that students mostly prefer game types of CDs in English learning. Game types of CDs are effective for improving listening competnece. However, the result of the studies represents that the effectiveness of the CDs are different for individuals. Educational effects are more significant for students who have had experience for using CDs than that of students who do not have experience. Moreover, other student variables are significantly related to the effectiveness of using CD-Rom titles in elmentary English education. Thus, teachers are required to analyze what types of CD-Rom titles should be used in their lessons and to whom they use before they use CDs in their English class.

Midazolam 투여시 선행성 건망증 발현에 관한 연구

이정하,김미라,이난영,이상호 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

최근 의식하 진정요법에 많이 이용되고 있는 Midazolam의 임상적 효과로는 항불안, 진정, 수면, 항경련, 근이완, 기억상실 등이 있는데, 이중 기억상실효과는 주로 선행성 건망증(anterograde amnesia)으로 약물투여 후 약물의 작용이 지속되는 동안에 경험한 일들을 기억하지 못하는 현상이다. 이는 시술 중 환자의 협조를 얻을 수 있으면서도 시술 후 환자는 시술중의 불편한 과정을 기억하지 못하는 효과를 얻을 수 있다는 임상적 장점이 있다. 본 연구는 Midazolam 투여 시 선행성 건망증의 발현 양상을 평가할 목적으로 약물에 대한 기왕력이 없으며 미국 마취학회 전신상태 평가기준에 따라 1등급으로 판정된 조선대학교 치과대학에 재학중인 학생 15명을 대상으로 위약, 경구용 Midazolam 투여, 주사용 Midazolam의 비강내 투여 후 Snograss와 Vanderwart에 의해 표준화된 260개 그림 시리즈를 이용하여 선행성 건망증의 발현정도를 평가한 결과 다음과 같은 결론을 얻었다. 1. Midazolam의 경구투여와 비강내 투여 시 선행성 건망증의 정도가 위약에 비해 유의하게 높았다 (P<0.01). 2. Midazolam의 비강내 투여시 선행성 건망증의 정도가 경구투여보다 높았으나 유의한 차이는 없었다(P>0.01). 3. Midazolam의 경구투여와 비강내 투여 모두 약물투여 15분 후부터 선행성 건망증을 보이기 시작하여 경구투여시는 45분, 비강내 투여시는 30분에 가장 높은 효과를 보였다. 4. 약물투여 30분이 지나면서 비강내 투여보다 경구투여에서 선행성 건망증의 정도와 진정정도가 더 높았으나 유의한 차이는 없었다(P>0.01). 본 결과로 미루어 보아 Midazolam을 투여한 후 선행성 건망증은 경구투여와 비강내 투여 모두 약물투여 15분 후부터 나타났으며 30분 전까지는 비강내 투여에서 30분이 지나면 경구투여에서 더 높은 효과를 보이는 경향이 관찰된 바, 치과치료시 Midazotam의 경구투여와 비강내 투여를 이용한 의식진정요법을 시행함에 있어 국소마취 등의 고통스러운 과정은 최소약물투여 15분 후 시행하는 것이 향후 치과진료에 대한 환자의 긍정적 자세를 유도하는 데 도움이 되리라 사료된다. The purpose of this study was to assess the anterograde amnesic effect of Midazolam administrated by in-tranasal and oral route. Fifteen healthy volunteers(ASA I) were administrated with placebo, oral Midazolam (15mg), intranasal Midazolam (0.25mg/1kg) every 2 weeks. First picture cards were shown to volunteers before medication. At 15, 30, 45 minutes, other picture cards were shown to volunteers. BP, SpO₂ and sedation scores were measured. After 24 hours, the volunteers were questioned about their memory of pictures. To assess amnesic effect, recall and recognition test were performed using a series of picture cards designed for this purpose. The obtained results were as follows; 1. Compared to placebo, Midazolam group(oral and intranasal) experienced a significant anterograde amnesic effect (P<0.01). 2. There was no difference between oral and intranasal Midazolam (P>0.01). 3. Anterograde amnesic effect of oral and intranasal Midazolam group began at 15minutes. It became increasingly, oral Midazolam group experienced extremely amnesic effect at 45minutes, intranasal Midazolam group was at 30minutes. 4. After 30minutes of Midazolam administration, anterograde amnesia of oral Midazolam group is more effective than intranasal Midazolam group.

한국인 사춘기성 성장기에서의 수완부 골성숙도와 치아발육에 관한 연구

이상호,이미라 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.1

The purpose of this study was to investigate the relationship of the development of teeth and the bone maturity of hand-wrist during pubertal growth period in Korean. The subjects used in this study were 295 children (male 147, female 148), ranging from 8 to 16 years of age, with good general health, none of whom had received orthodontic therapy. Panoramic radiographs and hand-wrist radiographs were used to evaluate to stage of teeth development and the bone marurity of hand-wrist and their relationship. The results were as follows; 1. In bone maturity of hand-wrist, a definite trend toward acceleration among females was evident, and in tooth maturation, a definite trend toward acceleration among females was evident in upper and lower canines. 2. In stage 3 of bone maturity of hand-wrist(onset of pubertal growth), the percentage distribution of developmental stage G in lower canine was the most most high(77%). The two es of the above were highly coincided with each other. 3. In spite of variety in correlation between teeth development and bone maturity of hand-wrist, there was the relatively close correlation in the lower first premolar in male(R=0.57) and the lower second premolar in female(R-0.73). 4. There was the highly close relationship between chronologic age and bone maturity of hand-wrist(R=0.82 in male, R=0.84 in female). However the correlation between chronologic age and teeth maturation was low, and different among various teeth(R=0.11-0.52).

회귀분석을 이용한 A17075의 표면거칠기에 관한 엔드밀 가공조건의 상관관계 추정(Ⅰ)

이상재,전환영,류미라,박흥식 東亞大學交附設 生産技術硏究所 2005 生産技術硏究所硏究論文集 Vol.10 No.-

End-milling have been used widely in industrial system because it is effective to a material manufacturing with various shape. Recently the end-milling processing is needed the high-precise technique with good surface roughness and rapid time in precision machine part and electronic part. The optimum surface roughness has an effect on end-milling condition such as, cutting direction, spindle speed, feed rate and depth of cut, and so on. Therefore this study was carried out to presume for mutual relation of end-milling condition to get the optimum surface roughness by regression analysis. The results shown that coefficient of determination(R^(2)) of regression equation has a fine reliability of 88.2% and regression equation of surface rough is made by regression analysis.

노인의 안전의식과 안전사고 발생에 관한 연구

이경자,이미라,조윤희 노인간호학회 2008 노인간호학회지 Vol.10 No.1

Purpose: The purpose of this study was to investigate safety awareness and accidents in elders. Method: A descriptive survey was done to determine the incidence of accidents and level of safety awareness. The participants were 300 elders 65 or over living in Seoul. Data were collected from May 1 to July 31. 2007 through personal interview using a structured questionnaire. Descriptive statistics. t-test, ANOVA. and logistic regression were used to analyze the data. Results: The results are as follows. 1. The average safety awareness score was 44.57 of a maximum of 54. 2. Most of accidents were caused by falls (25.7%). followed by bumping into something (11.0%). and traffic accidents (3.6%). 3. There was no statistically significant difference between accidents and safety awareness level. 4. There were significant correlations between incidence of burns and health status (p<.05). and incidence of bumps and age (p<.Ol) and incidence of arthritis (p<.05), 5. There were significant correlations among safety awareness level and age (p<.05). health status (p(.01). and incidence of hypertension (p<.Ol). Conclusion: These results suggest that safety education programs are necessary to achieve safety for elders and to prevent accidents.