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Khan, Muhammad,Maryam, Amara,Mehmood, Tahir,Zhang, Yaofang,Ma, Tonghui Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16
Multidrug resistance is a principal mechanism by which tumors become resistant to structurally and functionally unrelated anticancer drugs. Resistance to chemotherapy has been correlated with overexpression of p-glycoprotein (p-gp), a member of the ATP-binding cassette (ABC) superfamily of membrane transporters. P-gp mediates resistance to a broad-spectrum of anticancer drugs including doxorubicin, taxol, and vinca alkaloids by actively expelling the drugs from cells. Use of specific inhibitors/blocker of p-gp in combination with clinically important anticancer drugs has emerged as a new paradigm for overcoming multidrug resistance. The aim of this paper is to review p-gp regulation by dietary nutraceuticals and to correlate this dietary nutraceutical induced-modulation of p-gp with activity of anticancer drugs.
Din Maryam Salah Ud,Gohar Umar Farooq,Mukhtar Hamid,Khan Ibrar,Morris John,Pornpukdeewattana Soisuda,Massa Salvatore 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.4
Irrational and injudicious use of antibiotics, easy availability of them as over-the-counter drugs in economically developing countries, and unavailability of regulatory policies governing antimicrobial use in agriculture, animals, and humans, has led to the development of multi-drug resistance (MDR) bacteria. The use of medicinal plants can be considered as an alternative, with a consequent impact on microbial resistance. We tested extracts of Piper longum fruits as new natural products as agents for reversing the resistance to antibiotics. Six crude extracts of P. longum fruits were utilized against a clinical isolate of multidrug-resistant Staphylococcus aureus.The antibiotic susceptibility testing disc method was used in the antibiotic resistance reversal analysis. Apart from cefoxitin and erythromycin, all other antibiotics used (lincosamides [clindamycin], quinolones [levofloxacin and ciprofloxacin], and aminoglycosides [amikacin and gentamicin]) were enhanced by P. longum extracts. The extracts that showed the greatest synergy with the antibiotics were EAPL (ethyl acetate [extract of] P. longum), n-BPL (n-butanol [extract of] P. longum), and MPL (methanolic [extract of] P. longum The results of this study suggest that P. longum extracts have the ability to increase the effectiveness of different classes of antibiotics and reverse their resistance. However, future studies are needed to elucidate the molecular mechanisms behind the synergy between antibiotic and phytocompound(s) and identify the active biomolecules of P. longum responsible for the synergy in S. aureus.
( Zahoor Khan ),( Maryam Shafique ),( Amir Zeb ),( Nusrat Jabeen ),( Sehar Afshan Naz ),( Arif Zubair ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.1
Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.
Andleeb Khan,Mohammad F. Alam,Tarique Anwer,Razan Ayoub,Rana J. H. Bahkali,Hina Rashid,Saeed Alshahrani,Marwa Qadri,Yousra Nomier,Maryam Sageer,Mohammad Suhail Akhter The Korean Society for Reproductive Medicine 2023 Clinical and Experimental Reproductive Medicine Vol.50 No.1
Objective: Human exposure to multiple xenobiotics, over various developmental windows, results in adverse health effects arising from these concomitant exposures. Humans are widely exposed to bisphenol A, and acetaminophen is the most commonly used over-the-counter drug worldwide. Bisphenol A is a well-recognized male reproductive toxicant, and increasing evidence suggests that acetaminophen is also detrimental to the male reproductive system. The recent recognition of male reproductive system dysfunction in conditions of suboptimal reproductive outcomes makes it crucial to investigate the contributions of toxicant exposures to infertility and sub-fertility. We aimed to identify toxicity in the male reproductive system at the mitochondrial level in response to co-exposure to bisphenol A and acetaminophen, and we investigated whether melatonin ameliorated this toxicity. Methods: Male Wistar rats were divided into six groups (n=10 each): a control group and groups that received melatonin, bisphenol A, acetaminophen, bisphenol A and acetaminophen, and bisphenol A and acetaminophen with melatonin treatment. Results: Significantly higher lipid peroxidation was observed in the testicular mitochondria and sperm in the treatment groups than in the control group. Levels of glutathione and the activities of catalase, glutathione peroxidase, glutathione reductase, and manganese superoxide dismutase decreased significantly in response to the toxicant treatments. Likewise, the toxicant treatments significantly decreased the sperm count and motility, while significantly increasing sperm mortality. Melatonin mitigated the adverse effects of bisphenol A and acetaminophen. Conclusion: Co-exposure to bisphenol A and acetaminophen elevated oxidative stress in the testicular mitochondria, and this effect was alleviated by melatonin.
AHMED, Wahab,KHAN, Hadi Hassan,RAUF, Abdul,ULHAQ, SM Nabeel,BANO, Safia,SARWAR, Bilal,HUDA, Shams ul,KHAN, Mirwaise,WALI, Ahmed,DURRANI, Maryam Najeeb Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.3
In the last few years, there is growing interest in the field of simultaneous equation estimation in finance due to the endogeneity problem caused by measurement errors, simultaneity, or omitted variables. This study aims to discuss the endogeneity problem in corporate financing decisions and investigate the interrelationship of financial decision-making such as investment decision, dividend decision, and external financing decision in Pakistan Stock Exchange (PSX) using two-stage least squares (2SLS) and generalized method of moment (GMM) estimation. The Bruech-Pagan test shows that the data has no heteroskedasticity issue and 2SLS is a better approach in the context of this study as compared to the GMM approach, and internal instruments are also sufficiently strong and valid. The three financial decision-making attributes are not jointly determined, and the dividend is influenced by one-sided investment. In the emerging stock market context, external financing and investment are not inter-related and did not affect each other. The question of whether the simultaneous equation estimation can be useful in the context of the emerging stock markets and newly-growing firms remains unanswered. The inclusive evidence shows that the theoretical link in the emerging stock market is difficult to prove like in developed stock markets.
Nuzhat Noureen,Saadia Khan,Asim Khursheed,Imran Iqbal,Moallah Maryam,Syed Muhammad Sharib,Neeta Maheshwary 대한신경과학회 2019 Journal of Clinical Neurology Vol.15 No.4
Background and Purpose Tere is sparsity of quality evidence for the use of drugs afer frst-line benzodiazepines in convulsive status epilepticus in children. Te aim of the study was to compare the clinical efcacy and safety of intravenous levetiracetam versus intravenous phenytoin as second-line drugs in the management of generalized convulsive status epilepticus in children. Methods Tis open-label randomized controlled trial was conducted in the Emergency Department of Te Children’s Hospital and Te Institute of Child Health, Multan, Pakistan over a period of 4 years and 6 months from January 2014 to June 2018. Tis study included 600 children with generalized convulsive status epilepticus: 300 in the 40 mg/kg levetiracetam group, and 300 in the 20 mg/kg phenytoin group. Cessation of a clinical seizure (seizure cessation rate) within 30 minutes afer the end of drug administration was the primary outcome in this study, and the presence or absence of adverse efects was noted as the secondary outcome. Data were analyzed using SPSS (version 20.0). Results Te children in the levetiracetam and phenytoin were aged 3.5±0.2 and 3.4±0.2 years (mean±SD), respectively, their seizure durations before the start of treatment were 25.1±0.6 and 23.8±0.4 minutes, and their treatment efficacies were 278/300 (92.7%) and 259/300 (83.3%). Levetiracetam was signifcantly more efective than phenytoin (p=0.012), with no signifcant diference in safety. Adverse events were observed in eight children in the phenytoin group. Conclusions Levetiracetam is signifcantly more efective than phenytoin for the treatment of convulsive status epilepticus in children who have failed to respond to benzodiazepines.
Electrochemical sensing of H2O2 using cobalt oxide modified TiO2 nanotubes
Ullah Rahim,Rasheed Muhammad Asim,Abbas Shafqat,Rehman Khalil-ul,Shah Attaullah,Ullah Karim,Khan Yaqoob,Bibi Maryam,Ahmad Mashkoor,Ali Ghafar 한국물리학회 2022 Current Applied Physics Vol.38 No.-
Cobalt oxide (Co3O4) modified anatase titanium dioxide nanotubes (ATNTs) have been investigated for the electrochemical sensing of hydrogen peroxide (H2O2). ATNTs have been synthesized by a two-step anodization process. ATNTs were then modified with Co3O4 employing chemical bath deposition method. The structure and morphology of ATNTs and their modification with Co3O4 has been confirmed by X-ray diffraction by scanning electron microscopy. H2O2 sensing has been studied in 0.1 M PBS solution, by cyclic voltammetry and amperometry. Variation in the peak positions and current densities was observed with addition of H2O2 for Co3O4 modified ATNTs. Sensitivity and limit of detection improved with modification of ATNTs with Co3O4 with precursor concentration up to 0.8 M. However, at higher precursor concentrations sensitivity and limit of detection toward H2O2 deteriorated. Co3O4 Modified ATNTS using 0.8 M precursor concentration are comparatively more suitable for H2O2 sensing applications due to the optimum formation of Co3O4/ATNTs heterojunctions.