Zn (II) removal by amino-functionalized magnetic nanoparticles: Kinetics, isotherm, and thermodynamic aspects of adsorption

Nahid Ghasemi,Maryam Ghasemi,Saleh Moazeni,Parisa Ghasemi,Njud S. Alharbi,Vinod Kumar Gupta,Shilpi Agarwal,Irina V. Burakova,Alexey G. Tkachev 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.62 No.-

In this study, a novel adsorbent, dioctylphetalate triethylenetetraamine magnetic nanoparticles (DOP-TETA-MNP) via a solvent-free procedure were synthesized for the removal of Zn (II) ions from aqueous solution. The morphological and anatomical characterization was investigated by various analytical techniques such as FTIR and SEM. Experimental data from evaluation of various influential parameters such as initial pH (2–7), initial ion concentration (5–20 mg/L), adsorbent dose (0.005–0.040 g), contact time (5–120 min) and temperature (308–333 K) of the solution were carried out using a batch adsorption method. DOP-TETA-MNP can be simply separated from aqueous solution with magnetic separations at low magnetic field. The kinetic data of Zn (II) ions were found to follow the pseudo-second-order kinetic model and the equilibrium data of ions sorption were well described by Langmuir isotherm model. The maximum monolayer sorption capacity of Zn (II) was estimated to be 24.21 mg/g at temperature of 333 K. Thermodynamic analyses show that the adsorption process is exothermic and spontaneous.

Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei

Amir Ghasemi,Sobhan Ghafourian,Sedighe Vafaei,Reza Mohebi,Maryam Farzi,Morovat Taherikalani,Nourkhoda Sadeghifard 질병관리본부 2015 Osong Public Health and Research Persptectives Vol.6 No.6

Objectives: In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY-α- amylase. Methods: Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protein. Amylolytic activity of proteins produced by transformed E. coli cells was detected by zymography, and the rate of active α-amylase with and without the intein tag in both soluble conditions and as inclusion bodies solubilized by 4M urea were measured. Results: Amylolytic activity of ~185,000 U/L of bacterial culture was observed from the soluble form of the protein using this system. Conclusion: These results indicate that this expression system was appropriate for the production of thermostable α-amylase.

Condition medium of cerebrospinal fluid and retinoic acid induces the transdifferentiation of human dental pulp stem cells into neuroglia and neural like cells

Sara Haratizadeh,Maryam Nazm Bojnordi,Shahram Darabi,Narges Karimi,Mehrdad Naghikhani,Hatef Ghasemi Hamidabadi,Morteza Seifi 대한해부학회 2017 Anatomy & Cell Biology Vol.50 No.2

Cerebrospinal fluid (CSF) contains several molecules which are essential for neurogenesis. Human dental pulp stem cells (hDPSCs) are putatively neural crest cell-derived that can differentiate into neurons and glial cells under appropriate neurotrophic factors. The aim of this study was to induce differentiation of hDPSCs into neuroglial phenotypes using retinoic acid (RA) and CSF. The hDPSCs from an impacted third molar were isolated by mechanical and digestion and cultured. The cells have treated by 10 –7 μM RA (RA group) for 8 days, 10% CSF (CSF group) for 8 days and RA with CSF for 8 days (RA/ CSF group). Nestin, microtubule-associated protein 2 (MAP2), and glial fibrillary acidic protein immunostaining were used to examine the differentiated cells. Axonal outgrowth was detected using Bielschowsky’s silver impregnation method and Nissl bodies were stained in differentiated cells by Cresyl violet. The morphology of differentiated cells in treated groups was significantly changed after 3–5 days. The results of immunocytochemistry showed the presence of neuroprogenitor marker nestin was seen in all groups. However, the high percentage of nestin positive cells and MAP2, as mature neural markers, were observed at the pre-induction and induction stage, respectively. Nissl bodies were detected as dark-blue particles in the cytoplasm of treated cells. Our findings showed the RA as pre-inducer and CSF as inducer for using in vitro differentiation of neuron-like cells and neuroglial cells from hDPSCs.

DNA Binding Studies and Cytotoxicity of the Novel 1,10-phenanthroline Palladium(II) Complexes of Dithiocarbamate Derivatives

Hassan Mansouri-Torshizi,Maryam Saeidifar,Zahra Yekke Ghasemi,Mahmood Khastan,Adeleh Divsalar,Ali Akbar Saboury 대한화학회 2011 대한화학회지 Vol.55 No.1

Two new palladium (II) complexes, [Pd (phen)(pip-dtc)]NO_3 and [Pd(phen)(mor-dtc)]NO_3, (where phen is 1,10-Phenantroline, pip-dtc is piperidinedithiocarbamate anion and mor-dtc is morpholinedithiocarbamate anion) have been synthesized and characterized by elemental analysis, spectroscopic studies (FT-IR, ^1 H NMR, UV-Vis) and conductance measurement. In these complexes, the dithiocarbamate ligands coordinate with Pd (II) center as bidentate with two sulfur atoms. These two complexes have been tested against chronic myelogenous leukemia cell line, K562. They show IC_(50) values less than cisplatin and thus the mode of binding of the complexes to calf thymus DNA (CT-DNA) were investigated by ultraviolet difference and fluorescence spectroscopy. They can denature DNA, exhibit cooperative binding and intercalate into DNA. Several binding and thermodynamic parameters are also described.

DNA-Binding and Thermodynamic Parameters, Structure and Cytotoxicity of Newly Designed Platinum(II) and Palladium(II) Anti-Tumor Complexes

Hassan Mansouri-Torshizi,Maryam Saeidifar,Fatemeh Khosravi,Adeleh Divsalar,Ali.Akbar Saboury,Zahra Yekke Ghasemi 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.3

The complexes [Pd(bpy)(Hex-dtc)]NO_3 and [Pt(bpy)(Hex-dtc)]NO_3 (bpy is 2,2'-bipyridine and Hex-dtc is hexyldithiocarbamato ligands) were synthesized and characterized by elemental analysis and spectroscopic studies. The cytotoxicity assay of the complexes has been performed on chronic myelogenous leukemia cell line, K562, at micromolar concentration. Both complexes showed cytotoxic activity far better than that of cisplatin under the same experimental conditions. The binding parameters of the complexes with calf thymus DNA (CT-DNA) was investigated using UV-visible and fluorescence techniques. They show the ability of cooperatively intercalating in CT-DNA. Gel filtration studies demonstrated that platinum complex could cleave the DNA. In the interaction studies between the Pd(II) and Pt(II) complexes with CT-DNA, several binding and thermodynamic parameters have been determined, which may provide deeper insights into the mechanism of action of these types of complexes with nucleic acids.

DNA-Binding and Thermodynamic Parameters, Structure and Cytotoxicity of Newly Designed Platinum(II) and Palladium(II) Anti-Tumor Complexes

Mansouri-Torshizi, Hassan,Saeidifar, Maryam,Khosravi, Fatemeh,Divsalar, Adeleh,Saboury, Ali.Akbar,Ghasemi, Zahra Yekke Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.3

The complexes [Pd(bpy)(Hex-dtc)]$NO_3$ and [Pt(bpy)(Hex-dtc)]$NO_3$ (bpy is 2,2'-bipyridine and Hex-dtc is hexyldithiocarbamato ligands) were synthesized and characterized by elemental analysis and spectroscopic studies. The cytotoxicity assay of the complexes has been performed on chronic myelogenous leukemia cell line, K562, at micromolar concentration. Both complexes showed cytotoxic activity far better than that of cisplatin under the same experimental conditions. The binding parameters of the complexes with calf thymus DNA (CT-DNA) was investigated using UV-visible and fluorescence techniques. They show the ability of cooperatively intercalating in CT-DNA. Gel filtration studies demonstrated that platinum complex could cleave the DNA. In the interaction studies between the Pd(II) and Pt(II) complexes with CT-DNA, several binding and thermodynamic parameters have been determined, which may provide deeper insights into the mechanism of action of these types of complexes with nucleic acids.

Million Visual Analogue Scale Questionnaire: Validation of the Persian Version

Hesam Akbari,Mohammad Ghasemi,Taha Yegani,Mohammad Gholami- Fesharaki, PhD,Maryam Saraei,Yalda Barsam,Hamed Akbari 대한척추외과학회 2019 Asian Spine Journal Vol.13 No.2

Study Design: Descriptive cross-sectional study. Purpose: To validate the Persian version of the Million Visual Analogue Scale Questionnaire (MVAS), a self-administered low back pain (LBP) questionnaire. Overview of Literature: The majority of LBP questionnaires translated into Persian evaluate the impact of LBP on daily living. The MVAS is one of the most commonly used self-administered LBP questionnaires, and was developed to assess a different direction and effect of activities of daily living on LBP intensity. Methods: The questionnaire was translated into Persian with the forward-backward method and was administered to 150 patients randomly sampled from an occupational medicine clinic in Tehran in 2017. Results: Cronbach’s alpha for all subscales ranged between 0.670 and 0.799. Confirmatory factor analysis showed adequate construct validity of the Persian version of the MVAS, with root mean square error of approximation 0.046, goodness of fit index 0.902, and comparative fit index 0.969. Other indexes were satisfactory. Conclusions: The Persian MVAS is a valid and reliable instrument that can assess the effect of various daily activities on the intensity of LBP.

Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells

Ghazaleh Goudarzi,Hatef Ghasemi Hamidabadi,Maryam Nazm Bojnordi,Azim Hedayatpour,Ali Niapour,Maria Zahiri,Forouzan Absalan,Shahram Darabi 대한해부학회 2020 Anatomy & Cell Biology Vol.53 No.3

Human dental pulp stem cells (hDPSCs) could be differentiated into neuron like-cells under particular microenvironments. It has been reported that a wide range of factors, presented in cerebrospinal fluid (CSF), playing part in neuronal differentiation during embryonic stages, we herein introduce a novel culture media complex to differentiate hDPSCs into neuron-like cells. The hDPSCs were initially isolated and characterized. The CSF was prepared from the Cisterna magna of 19-day-old Wistar rat embryos, embryonic cerebrospinal fluid (E-CSF). The hDPSCs were treated by 5% E-CSF for 2 days, then neurospheres were cultured in DMEM/F12 supplemented with 10⁻⁶ μm retinoic acid (RA), glial-derived neurotrophic factor and brain-derived neurotrophic factor for 6 days. The cells which were cultured in basic culture medium were considered as control group. Morphology of differentiated cells as well as process elongation were examined by an inverted microscope. In addition, the neural differentiation markers (Nestin and MAP2) were studied employing immunocytochemistry. Neuronal-like processes appeared 8 days after treatment. Neural progenitor marker (Nestin) and a mature neural marker (MAP2) were expressed in treated group. Moreover Nissl bodies were found in the cytoplasm of treated group. Taking these together, we have designed a simple protocol for generating neuron-like cells using CSF from the hDPSCs, applicable for cell therapy in several neurodegenerative disorders including Alzheimer’s disease.

DNA Binding Studies and Cytotoxicity of the Novel 1,10-phenanthroline Palladium(II) Complexes of Dithiocarbamate Derivatives

Mansouri-Torshizi, Hassan,Saeidifar, Maryam,Ghasemi, Zahra Yekke,Khastan, Mahmood,Divsalar, Adeleh,Saboury, Ali Akbar Korean Chemical Society 2011 대한화학회지 Vol.55 No.1

Two new palladium (II) complexes, [Pd (phen)(pip-dtc)]$NO_3$ and [Pd(phen)(mor-dtc)]$NO_3$, (where phen is 1,10-Phenantroline, pip-dtc is piperidinedithiocarbamate anion and mor-dtc is morpholinedithiocarbamate anion) have been synthesized and characterized by elemental analysis, spectroscopic studies (FT-IR, $^1H$ NMR, UV-Vis) and conductance measurement. In these complexes, the dithiocarbamate ligands coordinate with Pd (II) center as bidentate with two sulfur atoms. These two complexes have been tested against chronic myelogenous leukemia cell line, K562. They show $IC_{50}$ values less than cisplatin and thus the mode of binding of the complexes to calf thymus DNA (CT-DNA) were investigated by ultraviolet difference and fluorescence spectroscopy. They can denature DNA, exhibit cooperative binding and intercalate into DNA. Several binding and thermodynamic parameters are also described. 두 종류의 새로운 팔라디움(II) 착물인 [Pd(phen)(pip-dtc)]$NO_3$와 [Pd(phen)(mor-dtc)]$NO_3$ (여기서 phen은 1,10-페난트롤린, pip-dtc은 피페리딘디티오카르바메이트 음이온, mor-dtc은 모르폴린디티오카르바메이트 음이온을 가리킨다)를 합성하고 원소분석, 분광법(FT-IR, $^1H$ NMR, UV-Vis) 및 전기전도도 측정을 이용하여 특성을 조사하였다. 이 착물들에서, 디티오카르베이트 리간드는 팔라디움 (II) 중심과 두 개의 황 원자와 이중으로 배위 결합을 하고 있다. 이 착물의 세포독성을 K562 세포주를 이용하여 조사하였을 때 $IC_{50}$ 값이 시스플라틴보다 작았기 때문에 착물들의 송아지 흉선 DNA(CT-DNA)와의 결합 모드를 UV 흡광도 차이와 형광 분광법으로 조사하였다. 착물들은 DNA를 변형시키고, 협동결합을 하고, DNA에 끼어 들어간다. 또한 몇 가지 결합 및 열역학적인 변수들이 기술되었다.

Anesthesia and analgesia for common research models of adult mice

Siavash Ahmadi-Noorbakhsh,Mohammad Farajli Abbasi,Maedeh Ghasemi,Gholamreza Bayat,Nahid Davoodian,Ehsan Sharif-Paghaleh,Seyedeh Mahsa Poormoosavi,Melika Rafizadeh,Maryam Maleki,Hesamaddin Shirzad-Aski 한국실험동물학회 2022 Laboratory Animal Research Vol.38 No.4

Anesthesia and analgesia are major components of many interventional studies on laboratory animals. However, various studies have shown improper reporting or use of anesthetics/analgesics in research proposals and published articles. In many cases, it seems “anesthesia” and “analgesia” are used interchangeably, while they are referring to two different concepts. Not only this is an unethical practice, but also it may be one of the reasons for the proven suboptimal quality of many animal researches. This is a widespread problem among investigations on various species of animals. However, it could be imagined that it may be more prevalent for the most common species of laboratory animals, such as the laboratory mice. In this review, proper anesthetic/analgesic methods for routine procedures on laboratory mice are discussed. We considered the available literature and critically reviewed their anesthetic/analgesic methods. Detailed dosing and pharmacological information for the relevant drugs are provided and some of the drugs’ side effects are discussed. This paper provides the necessary data for an informed choice of anesthetic/analgesic methods in some routine procedures on laboratory mice.