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Maruthamuthu, Murali kannan,Hong, Jiyeon,Arulsamy, Kulandaisamy,Somasundaram, Sivachandiran,Hong, SoonHo,Choe, Woo-Seok,Yoo, Ik-Keun Springer-Verlag 2018 Bioprocess and biosystems engineering Vol.41 No.4
<P>Peptide-displaying Escherichia coli cells were investigated for use in adsorptive removal of bisphenol A (BPA) both in Luria-Bertani medium including BPA or ATM thermal paper eluted wastewater. Two recombinant strains were constructed with monomeric and dimeric repeats of the 7-mer BPA-binding peptide (KSLENSY), respectively. Greater than threefold increased adsorption of BPA [230.4 A mu mol BPA per g dry cell weight (DCW)] was found in dimeric peptide-displaying cells compared to monomeric strains (63.4 A mu mol per g DCW) in 15 ppm BPA solution. The selective removal of BPA from a mixture of BPA analogs (bisphenol F and bisphenol S) was verified in both monomeric and dimeric peptide-displaying cells. The binding chemistry of BPA with the peptide was assumed, based on molecular docking analysis, to be the interaction of BPA with serine and asparagine residues within the 7-mer peptide sequence. The peptide-displaying cells also functioned efficiently in thermal paper eluted wastewater containing 14.5 ppm BPA.</P>
Maruthamuthu, S,Chandrasekaran, J,Manoharan, D,Karthick, SN,Kim, Hee‐,Je,Saravanakumar, B John Wiley Sons, Ltd 2016 Polymer international Vol.65 No.5
<P><B>Abstract</B></P><P>Nanocolloidal polypyrrole (PPy):poly(styrene sulfonate) (PSS) particles were synthesized by chemical oxidative polymerization using 15 wt% of PSS. The highly processable polymer composite (PPy:PSS) was spin‐coated at 4000 rpm on fluorine‐doped tin oxide glass and subsequently employed as a counter electrode (CE) for dye‐sensitized solar cells (DSCs). PPy:PSS multilayer (one, three, five) CEs were treated with CuBr<SUB>2</SUB> salt, which enhances the efficiency of the DSCs. Optical studies reveal that a bulkier counterion hinders interchain interactions of PPy which on salt treatment shows a moderate redshift in absorption maxima. Salt‐treated PPy:PSS films exhibit lower charge transfer resistance, higher surface roughness and better catalytic performance for the reduction of I<SUB>3</SUB><SUP>−</SUP>, when compared with untreated films. The improved catalytic performance of salt‐treated PPy:PSS multilayer films is attributed to charge screening and conformational change of PPy, along with the removal of excess PSS. Under standard AM 1.5 sunlight illumination, salt treatment is shown to boost the efficiency of multilayer PPy:PSS composite film‐based DSCs, leading to enhanced power conversion efficiency of 6.18, 6.33 and 6.37% for one, three and five layers, respectively. These values are significantly higher (<I>ca</I> 50%) than those for corresponding devices without CuBr<SUB>2</SUB> salt treatment (3.48, 2.90 and 2.01%, respectively). © 2016 Society of Chemical Industry</P>
Maruthamuthu, Murali kannan,Selvamani, Vidhya,Nadarajan, Saravanan Prabhu,Yun, Hyungdon,Oh, You-Kwan,Eom, Gyeong Tae,Hong, Soon Ho Springer-Verlag 2018 Journal of industrial microbiology & biotechnology Vol.45 No.1
<P>In a cell-surface display (CSD) system, successful display of a protein or peptide is highly dependent on the anchoring motif and the position of the display in that anchoring motif. In this study, a recombinant bacterial CSD system for manganese (Mn) and cobalt (Co) recovery was developed by employing OmpC as an anchoring motif on three different external loops. A portion of Cap43 protein (TRSRSHTSEG)(3) was employed as a manganese and cobalt binding peptide (MCBP), which was fused with OmpC at three different external loops. The fusions were made at the loop 2 [fusion protein-2 (FP2)], loop 6 (FP6), and loop 8 (FP8) of OmpC, respectively. The efficacy of the three recombinant strains in the recovery of Mn and Co was evaluated by varying the concentration of the respective metal. Molecular modeling studies showed that the short trimeric repeats of peptide probably form a secondary structure with OmpC, thereby giving rise to a difference in metal recovery among the three recombinant strains. Among the three recombinant strains, FP6 showed increased metal recovery with both Mn and Co, at 1235.14 (1 mM) and 379.68 (0.2 mM) mu mol/g dry cell weight (DCW), respectively.</P>
Impact of Ammonia Producing Bacillus sp. on Corrosion of Cupronickel alloy 90:10
S. Maruthamuthu,P. Dhandapani,S. Ponmariappan,배정효,N. Palaniswamy,Pattanathu K.S.M. Rahman 대한금속·재료학회 2009 METALS AND MATERIALS International Vol.15 No.3
The objectives of the present investigation were to characterize ammonia-producing bacteria (Bacillus sp.) and study its impact on biodeterioration of cupronickel alloy 90:10. in a nitrogen free environment. It is well known that iron sulphate and molybdenum are good inhibitors when used in a cooling water system. The interactions between inhibitor and ammonia producing bacteria on the corrosion of cupronickel 90:10 were studied. The predominated ammonia producing bacteria Bacillus spp. (AG1-EU202683; AG2-EU202684; AG3-EU202685 and AG4-EU202686) were identified by 16S rRNA gene sequencing from the biofilm. These bacteria fixed with atmospheric nitrogen for their cell protein synthesis and converted into ammonia. Ammonia enhanced pH and ammonical solution were formed in the presence of Bacillus spp. which acted as an etchant. The presence of some anodic spots in the presence of bacteria was affected by ammonia and then underwent pitting corrosion. The present study reveals that Bacillus spp. encourage intergranular attack without any stress in the cooling water system. The objectives of the present investigation were to characterize ammonia-producing bacteria (Bacillus sp.) and study its impact on biodeterioration of cupronickel alloy 90:10. in a nitrogen free environment. It is well known that iron sulphate and molybdenum are good inhibitors when used in a cooling water system. The interactions between inhibitor and ammonia producing bacteria on the corrosion of cupronickel 90:10 were studied. The predominated ammonia producing bacteria Bacillus spp. (AG1-EU202683; AG2-EU202684; AG3-EU202685 and AG4-EU202686) were identified by 16S rRNA gene sequencing from the biofilm. These bacteria fixed with atmospheric nitrogen for their cell protein synthesis and converted into ammonia. Ammonia enhanced pH and ammonical solution were formed in the presence of Bacillus spp. which acted as an etchant. The presence of some anodic spots in the presence of bacteria was affected by ammonia and then underwent pitting corrosion. The present study reveals that Bacillus spp. encourage intergranular attack without any stress in the cooling water system.
Vidhya Selvamani,Murali Kannan Maruthamuthu,Kulandaisamy Arulsamy,엄경태,홍순호 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.6
Methylobacterium organophilum XX is a type II facultative methylotroph that can grow on methanol. In M. organophilum XX, the MxcQ/MxcE two-component system (TCS) is involved in methanol metabolism. EnvZ/OmpR in E. coli TCS was exploited to develop a methanol biosensor by engaging the MxcQ/MxcE TCS system. The MxcQZ/ OmpR methanol sensing chimeric TCS was constructed by integrating the sensing domain of M. organophilum MxcQ with the transmitter domain of E. coli EnvZ. The response regulator of the chimeric TCS system is OmpR, which regulates the expression of the ompC and gfp. The expression of ompC was monitored by real-time quantitative PCR analysis. The expression of gfp also confirmed the expression of the ompC. The maximum expression of ompC and gfp occurred with 0.05% of methanol, and the expression started to decline with further increases in methanol concentration. This system delivers rapid detection of methanol in the environment.
Ilangovan, A.,Muralidharan, S.,Maruthamuthu, S. Korean Chemical Society 2011 대한화학회지 Vol.55 No.6
A systematic study of Knoevenagel reaction and Nazarov cyclization was made on variety of less reactive carbonyl compounds such as ${\beta}$-ketoesters, 1,3-diketones and cyclic active methylene compounds using $Yb(OTf)_3$ as the catalyst. Recycling study confirms reusability of the catalyst without much loss of activity.
홍순호,( Murali Kannan Maruthamuthu ),석정욱,정재훈 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Chimeric sensor kinase (SK)-based biosensor was constructed using Pseudomonas putida AauS. The AauZ chimera SK was constructed by integration of the sensing domain of AauS with the catalytic domain of EnvZ to control the expression of the ompC gene in response to acidic amino acids. Real-time quantitative PCR and GFP fluorescence studies showed increased ompC gene expression and GFP fluorescence as the concentration of acidic amino acids increased. These data suggest that AauS-based recombinant E. coli can be used as a bacterial biosensor of acidic amino acids. By employing the chimeric SK strategy, various bacteria biosensors for use in the development of biochemical-producing recombinant microorganisms can be constructed. <sup>**</sup>This work was supported by a grant from the Next-Generation Bio- Green 21 Program (SSAC, grant number: PJ011116) by RDA and Basic Science Research Program by the Ministry of Education (NRF-2014R 1A1A2054726).