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Yahaya, M.S.,Kimura, A.,Harai, J.,Nguyen, H.V.,Kawai, M.,Takahashi, J.,Matsuoka, S. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.12
The evaluation of structural carbohydrate losses and its effect on silages digestibility in alfalfa (Medicago sativa L.) and orchardgrass (Dactylis glomerata L.) was studied during 5, 21 and 56 days ensiling. About 70 and 60 kg fresh matter of the two forages were ensiled in 9 silos of 120 L capacity. The digestion trials were conducted in two phases using the two grasses in two $4{\times}4$ Latin square design according to the four treatments being the grass and the three silages. There were no differences in the DM and CP contents resulting from 5 to 56 days ensiling in both forages. The water-soluble carbohydrates (WSC), hemicelllose, pectin, and energy were slightly reduced and appeared lower in 56 days silage. The ether extract and cellulose contents slightly increased as the ensiling process advanced in the two species. Hemicellulose losses of 29 and 41 g/kg DM were obtained in alfalfa and orchardgrass, respectively, 56 days after ensiling. While the cellulose losses in both species were very little, compared to that for hemicellulose, the pectin losses, 56 days after ensiling were 15 and 12 g/kg DM in alfalfa and orchardgras respectively. The total structural carbohydrates lost (ie., hemicellulose + cellulose + pectin) in g/kg DM of fresh material forage ensiled, is about four fifths the amount lost by WSC, in alfalfa and about two thirds, in orchardgrass, by 21 days ensiling after the activity of microorganism terminated, indicating that appreciable amount was used as substrate for silage fermentation. Ensiling alfalfa and orchardgrass for 0, 5, 21 and 56 days maintained a decreasing trend of 83.8, 82.5, 79.3 and 78.9% digestibility in alfalfa and 80.5, 77.0, 77.1 and 76.4% digestibility in orchardgrass. While the digestibility of cellulose and ether extract increased in silage in both species, the digestible energy values in silage were reduced from 2.6 to 2.3 and 2.9 to 2.7 Mcal/kg DM respectively in alfalfa and orchard during 5-56 days ensiling.
Electrostatic Electron Cyclotron Harmonic Waves as a Candidate to Cause Pulsating Auroras
Fukizawa, M.,Sakanoi, T.,Miyoshi, Y.,Hosokawa, K.,Shiokawa, K.,Katoh, Y.,Kazama, Y.,Kumamoto, A.,Tsuchiya, F.,Miyashita, Y.,Tanaka, Y. ‐,M.,Kasahara, Y.,Ozaki, M.,Matsuoka, A.,Matsuda, S.,Hikish American Geophysical Union 2018 Geophysical research letters Vol.45 No.23
Yahaya, M.S.,Kibon, A.,Aregheore, E.M.,Abdulrazak, S.A.,Takahashi, J.,Matsuoka, S. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.4
In vitro and in vivo experiments were carried out to evaluate the nutritive value of three tropical browse species as assessed by DM and CP digestibility, and NDF and ADF degradability with incubation times (T) of 6, 12, 24 and 48h. During the in vivo digestibility experiment three male castrated sheep (age 16 - 25 months) with a mean liveweight of $11.5{\pm}0.9kg$ were placed in individual metabolism stalls and were allocated to one of the three browse species in a $3{\times}3$ Latin square design. The browse species were all leguminous and consisted of: Acacia sieberina (A. sieberina), Ficus polita (F. polita), and Ficus sycomorus (F. sycomorus). The mean DM and CP contents of F. polita were higher than for A. sieberina and F. sycomorus (p<0.05). In contrast the NDF and ADF contents of F. sycomorus were higher compared to the other species examined (p<0.05). The in vitro DM and CP digestibility, and NDF and ADF degradability observed at different stages of incubation were higher in F. polita followed by A. sieberina and F. sycomorus. The DM and CP digestibility at 48 h incubation were 72.92, 74.84 and 53.52% and 77.38, 77.68 and 63.64% for A. seiberina, F. polita and F. sycomorus, respectively. This shows that F. polita contains more soluble materials which ruminant can benefit from and hence has more feeding value. The fermentation of F. sycomorus was slower for all the nutrients evaluated due to the presence of more fibre. Similarly, higher in vivo digestibility coefficient of DM, CP, NDF, ADF and hemicellulose were observed for F. polita reflecting its higher values of CP, ether extract (EE) and hemicellulose associated with lower values of NDF and ADF. Higher DMI and daily gain were recorded in sheep during feeding of F. polita compared to the other species evaluated. The digestibility of all the nutrients examined were higher in the in vivo than in the in vitro trial except for CP and DM. Sheep showed no visual signs of toxicity throughout the study periods. These results showed A. seiberina, F. polita and F. sycomorus can sustain sheep on a maintenance diet and could as well be used as a supplementary feed to low producing animals during the tropical dry season. Further research is needed to ascertain the viability of using these browse species on a long-term basis.
Yahaya, M.S.,Kawai, M.,Takahashi, J.,Matsuoka, S. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.2
This study determined the influence of moisture, ensiling time and their interactions on the losses of hemicellulose and cellulose during ensiling of orchardgrass. Orchardgrass containing 80 (HM), 70 (MM) and 55% (LM) moisture was ensiled in 3 laboratory silos of 500 ml capacity for 3, 7, 21 and 91 days. The dry matter (DM), water-soluble carbohydrates (WSC), hemicellulose and cellulose contents of the ensiled orchardgrass was lowered than that of the untreated grass regardless of moisture content. Ensiling orchardgrass for 91 days (d) decreased (p<0.01) hemicellulose contents from 19 to 15%, 20 to 15% and 18 to 12% and cellulose from 31 to 29%, 29 to 26% and 27 to 26% for LM, MM and HM silage, respectively. Results from fermentation of LM and MM silages were within acceptable guidelines except for butyric acid and ammonia after 3 weeks of ensiling of MM which appeared to be lower than ideal. The results of the fermentation of HM silages were poor showing higher concentration of acetic, propionic and butyric acids and traces of isovaleric, valeric and caproic acids with ammonia at all stage of time. While the DM losses from LM and MM silages over the ensiling period were acceptable, that for HM silage increased to 13% after 91 d ensiling, confirming a poor fermentation process occurred. The greatest WSC losses occurred within 7 d of ensiling and the lowest losses occurred after 3 weeks of ensiling. Except in HM silage, the hemicellulose and cellulose losses were highest (p<0.01) in the first 3 weeks of ensiling. Hemicellulose losses were between 19 and 22% and 4.2 and 5.9% up to 3 weeks and after 3 weeks of ensiling LM and MM silages, respectively. Cellulose losses were small. In contrast, hemicellulose losses after 3 weeks of ensiling of HM silage was about 50% higher than over the first 3 weeks possibly due to clostridial type fermentation. The results showed that increasing ensiling time of high moisture orchardgrass would result in the excessive losses of DM, WSC, hemicellulose and cellulose in the silage.
K. Iino,M. Kitano,M. Takeuchi,M. Matsuoka,M. Anpo 한국물리학회 2006 Current Applied Physics Vol.6 No.6
Advanced ion-beam techniques such as metal ion-implantation, ionized cluster beam (ICB) deposition and RF-magnetron sputtering (RF-MS) deposition were found to enable the development of unique titanium oxide photocatalyst materials which are able to absorb and work not only under UV but also visible or solar light irradiation. Thus prepared visible light-responsive TiO2 act as efficient photocatalysts for the NO decomposition reaction into N2 and O2 as well as the H2 and O2 evolution reaction from water.
Adhim, Z,Matsuoka, T,Bito, T,Shigemura, K,Lee, K-M,Kawabata, M,Fujisawa, M,Nibu, K,Shirakawa, T Nature Publishing Group 2011 The British journal of cancer Vol.105 No.3
<P><B>Background:</B></P><P>Although the anti-tumour effect of cyclooxygenase-2 (Cox-2) inhibitors in invasive bladder cancer has been confirmed, its mechanisms of action are unclear. Recently, the concept of an epithelial-to-mesenchymal transition (EMT) promoting carcinoma progression has been suggested, and a key feature of the EMT is the downregulation of E-cadherin. In this study, we investigated the effect of Cox-2 inhibitors on reversal EMT and tumour growth inhibition in bladder cancer cells.</P><P><B>Methods:</B></P><P>We used three Cox-2 inhibitors, etodolac, celecoxib and NS-398 and three human bladder cancer cell lines, T24, 5637 and KK47, in this study. T24 xenograft tumour mouse model was used in the <I>in vivo</I> study.</P><P><B>Results:</B></P><P>Within the clinical drug concentrations, only etodolac showed the <I>in vitro</I> growth inhibition in T24 not in the other cell lines. Etodolac reduced <I>SNAIL</I> mRNA and vimentin cell surface expression, and induced <I>E-cadherin</I> mRNA and E-cadherin cell surface expression, in T24. Etodolac also most strongly inhibited the cell migration of T24 <I>in vitro</I> and showed the highest tumour growth inhibition in T24 tumour <I>in vivo</I>.</P><P><B>Conclusion:</B></P><P>Etodolac at clinical doses exhibited induced <I>in vitro</I> and <I>in vivo</I> anti-tumour effects and reversal effect of EMT in T24. These results suggest that etodolac is a good candidate for an anti-tumour or chemopreventive reagent for high-grade bladder cancer.</P>
Plasma-Confinement Physics Study in Compact Helical System
stoichi Okamura,A. Fujisawa,A. Shimizu,C. Takahashi,C. Suzuki,H. Iguchi,H. Nakano,H. Matsushita,K. Nagaoka,K. Matsuo,K. Ida,K. Toi,K. Nakamura,K. Matsuoka,M. Yoshinuma,M. Isobe,M. Takeuchi,R. Ikeda,S. 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.III
In a CHS experiment, various types of transport barrier have been studied. An internal transport barrier (ITB) for electrons in a stellarator was first found in CHS, and the ITB for improved ion confinement was also found. H-mode (edge transport barrier) study in CHS showed many common features in transition to tokamak experiments, although the magnetic surface quantities are very different. Discharges with a combination of ETB and ITB were also found. Two heavy ion beam diagnostics in CHS showed a new direct measurement of zonal flow structure in toroidal confinement. The relation between the fluctuation and the transport barrier was also studied.