TRIM22 promotes the proliferation of glioblastoma cells by activating MAPK signaling and accelerating the degradation of Raf-1

Fei Xiaowei,Dou Ya-nan,Sun Kai,Wei Jialiang,Guo Qingdong,Wang Li,Wu Xiuquan,Lv Weihao,Jiang Xiaofan,Fei Zhou 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

The tripartite motif (TRIM) 22 and mitogen-activated protein kinase (MAPK) signaling pathways play critical roles in the growth of glioblastoma (GBM). However, the molecular mechanism underlying the relationship between TRIM22 and MAPK signaling remains unclear. Here, we found that TRIM22 binds to exon 2 of the sphingosine kinase 2 (SPHK2) gene. An ERK1/2-driven luciferase reporter construct identified TRIM22 as a potential activator of MAPK signaling. Knockout and overexpression of TRIM22 regulate the inhibition and activation of MAPK signaling through the RING-finger domain. TRIM22 binds to Raf-1, a negative regulator of MAPK signaling, and accelerates its degradation by inducing K48-linked ubiquitination, which is related to the CC and SPRY domains of TRIM22 and the C1D domain of Raf-1. In vitro and in vivo, an SPHK2 inhibitor (K145), an ERK1/2 inhibitor (selumetinib), and the nonphosphorylated mutant Raf-1S338A inhibited GBM growth. In addition, deletion of the RING domain and the nuclear localization sequence of TRIM22 significantly inhibited TRIM22-induced proliferation of GBM cells in vivo and in vitro. In conclusion, our study showed that TRIM22 regulates SPHK2 transcription and activates MAPK signaling through posttranslational modification of two critical regulators of MAPK signaling in GBM cells.

Three partial mitochondrial genomes from Ips (Coleoptera: Curculionidae, Scolytinae) contribute to the phylogeny of Scolytinae

Lv Fei,Yang Wen-Yan,Chen Zhi-Teng,Xu Qiang,Zhou Yi-Jing,Du Yu-Zhou 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

Partial mitogenomes of three species from the genus Ips (Coleoptera; Curculionidae), Ips grandicollis, Ips sexdentatus and Ips typographus were sequenced and analyzed. The three mitogenomes are 11,553-bp with 28 genes, 13,135-bp with 30 genes, and 13,502-bp with 30 genes, respectively. Their gene arrangements are consistent with that of Drosophila yakuba. The three Ips species shared the conserved overlapping sequence motif “ATGATAG” between ATP8 and ATP6, using TTG as start codon for ND1 genes and TAG as stop codon for ATP8 genes. All tRNA genes could fold into typical cloverleaf structures except for tRNASer1. Three tRNASer1 genes lack the DHU arm and instead formed the D-loop. The phylogenetic analyses indicate that Scolytinae is paraphyly and belongs to Curculionidae. The Eurasian I. sexdentatus is sister group to the North American I. grandicollis and the Eurasian I. typographus. The phylogeny of Ips based on COI genes indicates that Ips is paraphyly, Ips perroti and Orthotomicus suturalis are sister groups and Ips spinifer is grouped with (Orthotomicus chaokhao + Orthotomicus erosus).

How “Foreign Influencer” Present Compelling Chinese Stories--Take “Chris”, a short video blogger in Tik Tok, as an example

Liu Jian(Jian Liu),Fei Jingying(Jingying Fei),Lv Mingkang(Mingkang Lv) 아시아사회과학학회 2022 International Science Research Vol.2 No.3

In recent years, a large number of excellent foreign short video bloggers have been active on major social media platforms, comparing the differences between Chinese and Western cultures, introducing Chinese traditional culture and customs to followers, and becoming a new force in telling Chinese stories. This paper takes the short video blogger “Chris” and his short videos as the research object, and analyze how “foreign influencer” tells Chinese stories in the new media environment, which brings new inspiration for telling Chinese stories and spreading Chinese voices well in the new media environment.

ACOX1 destabilizes p73 to suppress intrinsic apoptosis pathway and regulates sensitivity to doxorubicin in lymphoma cells

( Fei-meng Zheng ),( Wang-bing Chen ),( Tao Qin ),( Li-na Lv ),( Bi Feng ),( Yan-ling Lu ),( Zuo-quan Li ),( Xiao-chao Wang ),( Li-ju Tao ),( Hong-wen Li ),( Shu-you Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.9

Lymphoma is one of the most curable types of cancer. However, drug resistance is the main challenge faced in lymphoma treatment. Peroxisomal acyl-CoA oxidase 1 (ACOX1) is the rate-limiting enzyme in fatty acid β-oxidation. Deregulation of ACOX1 has been linked to peroxisomal disorders and carcinogenesis in the liver. Currently, there is no information about the function of ACOX1 in lymphoma. In this study, we found that upregulation of ACOX1 promoted proliferation in lymphoma cells, while downregulation of ACOX1 inhibited proliferation and induced apoptosis. Additionally, overexpression of ACOX1 increased resistance to doxorubicin, while suppression of ACOX1 expression markedly potentiated doxorubicin-induced apoptosis. Interestingly, downregulation of ACOX1 promoted mitochondrial location of Bad, reduced mitochondrial membrane potential and provoked apoptosis by activating caspase-9 and caspase-3 related apoptotic pathway. Overexpression of ACOX1 alleviated doxorubicin-induced activation of caspase-9 and caspase-3 and decrease of mitochondrial membrane potential. Importantly, downregulation of ACOX1 increased p73, but not p53, expression. p73 expression was critical for apoptosis induction induced by ACOX1 downregulation. Also, overexpression of ACOX1 significantly reduced stability of p73 protein thereby reducing p73 expression. Thus, our study indicated that suppression of ACOX1 could be a novel and effective approach for treatment of lymphoma. [BMB Reports 2019; 52(9): 566-571]

Joint MR image reconstruction and super-resolution via mutual co-attention network

Lv Tiantong,Wu Fei,Wang Wanliang 한국CDE학회 2024 Journal of computational design and engineering Vol.11 No.1

In the realm of medical diagnosis, recent strides in deep neural network-guided magnetic resonance imaging (MRI) restoration have shown promise. Nevertheless, persistent drawbacks overshadow these advancements. Challenges persist in balancing acquisition speed and image quality, while existing methods primarily focus on singular tasks like MRI reconstruction or super-resolution (SR), neglecting the interplay between these tasks. To tackle these challenges, this paper introduces the mutual co-attention network (MCAN) specifically designed to concurrently address both MRI reconstruction and SR tasks. Comprising multiple mutual cooperation attention blocks (MCABs) in succession, MCAN is tailored to maintain consistency between local physiological details and global anatomical structures. The intricately crafted MCAB includes a feature extraction block, a local attention block and a global attention block. Additionally, to ensure data fidelity without compromising acquired data, we propose the channel-wise data consistency block. Thorough experimentation on the IXI and fastMRI dataset showcases MCAN’s superiority over existing state-of-the-art methods. Both quantitative metrics and visual quality assessments validate the enhanced performance of MCAN in MRI restoration. The findings underscore MCAN’s potential in significantly advancing therapeutic applications. By mitigating the trade-off between acquisition speed and image quality while simultaneously addressing both MRI reconstruction and SR tasks, MCAN emerges as a promising solution in the domain of magnetic resonance image restoration.

A Hierarchical Single-Surface Model for an Unsaturated Soil-Structure Interface

Fei Peng,Xin Li,Meng-fan Lv,Yong-Hui Li,Yuan-Cheng Guo 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.6

In this paper, according to the shear test of the interface between unsaturated soil and steel plate, the modified apparent roughness is used to describe interface roughness. And considering the influence of matrix suction and interface roughness, the stress variable is constructed to improve the yield and plastic potential functions. Then a modified hierarchical single-surface elastoplastic interface constitutive model (HISS-Unsaturated-Int model) is set up. The finite element calculation of HISS-Unsaturated-Int model is achieved by compiling and calling the user subroutine FRIC of ABAQUS. The interface shear test between unsaturated soil and structure with different water contents and the pull-out shear test between unsaturated soil and soil nail are simulated. The calculation results are consistent with those of experiments, which shows the modified interface constitutive model can effectively predict the shear mechanical properties of soil-structure interface under different soil moisture content, normal stress and interface roughness.

Differences and Visualization of University Students’ Physical Health in Different Grade Levels: Based on College Student Samples in Shanxi Province

Lv Tao,Wang Fei,Liu Sheng-jie 아시아건강운동학회 2020 Journal of Asian Society for Health & Exercise Vol.2 No.1

Based on national students physical health database, we selected 6531 students from some universities in China and divided them into different grade levels according to test results. SOM (Self-organizing Map) was used to visually cluster and qualitatively analyze the physical health parameters of male and female students in each score interval. The key influencing factors and relevant explaining variables of physical fitness were conducted in each region based on principle component analysis (PCA). The results showed that body weight and BMI were the most influencing factors on physical fitness for both male and female, as well as the common explanatory variables for physical fitness. Weight, standing long jump and 50-meter run were the main influencing factors for male students’ physical fitness, as well as the main explanatory factors for the score of physical test. Obvious variations were observed on the influencing factors and explanatory variables in each score levels for female. Weight was both the main influencing factor and explanatory variable for female physical fitness. The clustering results by PCA indicated the clustering characteristics with BMI and body weight were relatively stable, whereas other variables showed different clustering characteristics in different score levels. Our findings provide valuable information and guidance for regional physical activities without ignoring the overall physical quality improvement.

Analysis and Proofs of Properties in Intransitive Noninterference

Congdong Lv,Xiaoyong Li,Fei Li,Wei Ma 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.7

The goal of high assurance systems development by formal verification motivates the investigation of techniques whereby a systems design or implementation can be formally shown to satisfy a formal definition of security. The security of unwinding relations provides a proof method that has been applied to establish that a system satisfies noninterference properties, but it requires significant human ingenuity to define an unwinding relation that forms the basis for the proof, and typically also has involved manual driving (proof rule selection) of the theorem proving tool within which the proof is conducted. The property of purge-based definition proposed by Goguen and Meseguer, intransitive purge-based definition proposed by Haigh and Young, and some more definitions TA-secure, TO-secure, ITO-secure proposed by van der Meyden are considered in this paper. The property can be used in the proof of noninterference property without unwinding relations.

A Fast Surface Defect Detection Method based on Background Reconstruction

Chengkan Lv,Zhengtao Zhang,Fei Shen,Feng Zhang,Hu Su 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.3

In this paper, we propose an unsupervised background reconstruction method to detect defects on surfaces with unevenly distributed textures. An improved deep convolutional autoencoder is utilized to reconstruct the textured background of the original image as a defect-free reference. Specifically, a weighted loss function based on structural similarity (SSIM) is utilized to adapt to the unevenly distributed texture background and improve the reconstruction accuracy. Furthermore, combined with the reconstructed defect-free reference, a novel difference analysis method based on the discrete cosine transform (DCT) is given to accurately segment the defect regions from the original image. A series of experiments for the defect detection on mobile phone cover glass (MPCG) are conducted. The processing time for an image of 512 × 512 pixels is only 20 ms, which satisfies the requirement of online detection. The experimental results verify the effectiveness of the proposed method.

Simultaneous Single-Position Lateral Lumbar Interbody Fusion Surgery and Unilateral Percutaneous Pedicle Screw Fixation for Spondylolisthesis

Hui Lv,Yu Sheng Yang,Jian Hong Zhou,Yuan Guo,Hui Chen,Fei Luo,Jian Zhong Xu,Zhong Rong Zhang,Ze Hua Zhang 대한척추신경외과학회 2023 Neurospine Vol.20 No.3

Objective: To evaluate the clinical and radiological efficacy of a combine of lateral single screw-rod and unilateral percutaneous pedicle screw fixation (LSUP) for lateral lumbar interbody fusion (LLIF) in the treatment of spondylolisthesis. Methods: Sixty-two consecutive patients with lumbar spondylolisthesis who underwent minimally invasive (MIS)-TLIF with bilateral pedicle screw (BPS) or LLIF-LSUP were retrospectively studied. Segmental lordosis angle (SLA), lumbar lordosis angle (LLA), disc height (DH), slipping percentage, the cross-sectional areas (CSA) of the thecal sac, screw placement accuracy, fusion rate and foraminal height (FH) were used to evaluate radiographic changes postoperatively. Visual analogue scale (VAS) and Oswestry Disability Index (ODI) were used to evaluate the clinical efficacy. Results: Patients who underwent LLIF-LSUP showed shorter operating time, less length of hospital stay and lower blood loss than MIS-TLIF. No statistical difference was found between the 2 groups in screw placement accuracy, overall complications, VAS, and ODI. Compared with MIS-TLIF-BPS, LLIF-LSUP had a significant improvement in sagittal parameters including DH, FH, LLA, and SLA. The CSA of MIS-TLIF-BPS was significantly increased than that of LLIF-LSUP. The fusion rate of LLIF-LSUP was significantly higher than that of MIS-TLIF-BPS at the follow-up of 3 months postoperatively, but there was no statistical difference between the 2 groups at the follow-up of 6 months, 9 months, and 12 months. Conclusion: The overall clinical outcomes and complications of LLIF-LSUP were comparable to that of MIS-TLIF-BPS in this series. Compared with MIS-TLIF-BPS, LLIF-LSUP for lumbar spondylolisthesis represents a significantly shorter operating time, hospital stay and lower blood loss, and demonstrates better radiological outcomes to maintain lumbar lordosis, and reveal an overwhelming superiority in the early fusion rate.