Research Progress in Applying Proteomics Technology to Explore Early Diagnosis Biomarkers of Breast Cancer, Lung Cancer and Ovarian Cancer

Luo, Lu,Dong, Li-You,Yan, Qi-Gui,Cao, San-Jie,Wen, Xin-Tian,Huang, Yong,Huang, Xiao-Bo,Wu, Rui,Ma, Xiao-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

According to the China tumor registry 2013 annual report, breast cancer, lung cancer, and ovarian cancer are three common cancers in China nowadays, with high mortality due to the absence of early diagnosis technology. However, proteomics has been widespreadly implanted into every field of life science and medicine as an important part of post-genomics era research. The development of theory and technology in proteomics has provided new ideas and research fields for cancer research. Proteomics can be used not only for elucidating the mechanisms of carcinogenesis focussing on whole proteins of the tissue or cell, but also seeking the biomarkers for diagnosis and therapy of cancer. In this review, we introduce proteomics principles, covering current technology used in exploring early diagnosis biomarkers of breast cancer, lung cancer and ovarian cancer.

Role of a Novel Pyridostigmine Bromide-Phospholipid Nanocomplex in Improving Oral Bioavailability

Qun-you Tan,Jing-qing Zhang,Ni-ni Hu,Guo-dong Liu,Hua-feng Yin,Li Zhang,Hong Wang,Lu-yang Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

A novel pyridostigmine bromide (PB)-phospholipid nanocomplex (PBPLC) was prepared to increase the bioavailability of PB. A central composite design approach was employed for process optimization. The physicochemical properties of PBPLC were investigated by means of differential scanning calorimetry, ultraviolet spectroscopy, Fourier transformed infrared spectroscopy and the n-octano/water partition coefficient. The intestinal permeability of PBPLC was observed via a single pass intestinal perfusion in rats. After oral administration of PBPLC, the concentrations of PB at predetermined time points were determined by HPLC, and the pharmacokinetic parameters were computed by DAS 2.1.1 software. Multiple linear regression analysis for process optimization revealed that the optimal PBPLC was obtained when the values of X1, X2, and X3 were 8, 40oC, and 4 mg/mL, respectively. The average particle size and zeta potential of PBPLC with the optimized formulation were 204.60 nm and −25.12 mV, respectively. Non-covalent interactions between PB and phospholipids were found in the PBPLC. The n-octanol/water partition coefficient of PBPLC was substantially increased. PBPLC had better intestinal permeability in comparison with free PB. Mean plasma drug concentration-time curves of PBPLC and free PB after oral administration were both in accordance with the two-compartment open model. The values of pharmacokinetic parameters of PBPLC and free PB were the peak time (Tmax) 2 h vs 2 h, the maximum concentration (Cmax) 22.79 μg/mL vs 6.00 μg/mL, and the value of the area under the concentration vs time curve (AUC0-∞) 7128.21 μg·min/mL vs 1772.36 μg·min/mL, respectively. In conclusion, compared with free PB, PBPLC remarkably improves the oral bioavailability of PB, which is likely due to its higher lipophilicity and permeability.

선물의 역할(giver-receiver)에서 관계적 특성이 해석 수준(construal level) 선호도 차이에 관한 연구

유인식 ( You In Sik ),이동명 ( Lee Dong Myung ),진로 ( Chen Lu ) 한국소비자학회 2019 소비자학연구 Vol.30 No.4

선물의 선호도는 주는 사람과 받는 사람의 두가지 해석 수준에 따라 달라진다. 첫 번째 해석 수준은 가치와 목적에 치중하는 즉 왜(Why)에 가까운 바람직성(desirability)이며, 두 번째는 사용 수단과 용이성에 치중하는 즉 어떻게(how)와 밀접한 실행가능성(feasibility)으로 구분된다. 이에 본 연구에서는 선물을 주는 사람과 받는 사람에 따라 어떠한 해석 수준의 선물을 선호하는지를 살펴보았다. 또한 관계적 특성을 사회적 관계와 심리적 친밀도로 구분하여, 관계적 특성에 따라 어떠한 해석 수준의 선물을 선호하는지를 추가적으로 심도 있게 연구하였다. 선물을 주는 사람과 받는 사람의 상황 별로 각 150명을 설문하여 유효 응답자 291명을 대상으로 연구한 결과, 다음과 같은 연구결과를 도출하였다. 첫째, 관계적 특성을 고려하지 않은 상황에서는 미국의 선물 문화에 대한 선행연구와 동일하게, 선물을 주는 사람은 바람직성이 높은 선물을 선호하였으며 받는 사람은 실행가능성이 높은 선물을 선호하였다. 둘째, 친밀도가 높은 가족, 친척, 친구의 관계에서는 받는 사람과 주는 사람 모두 실행 가능성이 높은 선물을 선호하였다. 세 번째 친밀도가 낮은 사적인 관계인 경우 주는 사람과 받는 사람 모두 바람직성이 높은 선물을 선호하였으며 이는 한국의 선물 문화에서 공동체의식과 체면 의식이 반영된 의무적인 선물 상황에서 발생하는 것으로 추정되며 반면에 공적인 관계에서는 친밀도가 높은 경우 주는 사람과 받는 사람 모두 실행 가능성이 높은 선물을 선호하였으며 친밀도가 낮은 경우 주는 사람은 바람직성이 높은 선물을 받는 사람은 실행 가능성이 높은 선물을 선호하였다. 이러한 연구 결과는 관계 특성과 친밀도가 선물 해석 수준 선호도 차이가 있음을 규명하였고 학문적이고 실무적인 시사점을 제공하였다. Gift preference depends on the construal level of two people, giver and receiver. 1st construal level depends on the value and the purpose. In other words, desirability close to why. 2nd construal level is about means of use and convenience. So, this study observes which gift is preferred depends on giver and receiver as per the level of interpretation of gift. Also, by distinguishing the characteristic of relationship depends on social relationship and emotional closeness, additional in-depth study on gift preference is done. Study on 150 of receiver and giver respectively, 291 responses were selected, and we have the following results. 1st, without considering relationship, similar to gift culture in the States, giver prefers a gift with high desirability and receiver prefers a gift with high level of feasibility. 2nd, within close family, friends and friends, both receiver and giver prefers a gift with high level of feasibility. 3rd, in a personal relationship with low closeness, both giver and receiver prefer a gift with high desirability. This seems to reflect Korean gifting culture where community spirit and respect for each other exists. In public relations, between giver and receiver who are close prefer a gift with high feasibility while who are not close prefer a gift with desirability. This study reveals the level of relationship and the closeness affects the interpretation of gift preference and provides the academical implication.

Medicinal Chemistry : ERK1/2 antagonize AMPK-dependent regulation of FcεRI-mediated mast cell activation and anaphylaxis

( Seung Lark Hwang ),( Yue Lu ),( Xian Li Msc ),( Yong Deuk Kim ),( You Sook Cho ),( Yurn Dong Jahng ),( Jong Keun Son ),( Youn Ju Lee ),( Won Ku Kang ),( Yoshitaka Taketomi ),( Makoto Murakami ),( Ta 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

Background: Extracellular signal-regulated kinases 1/2 (ERK1/2) make important contributions to allergic responses via their regulation of degranulation, eicosanoid production, and cytokine expression by mast cells, yet the mechanisms underlying their positive effects on FcεRI-dependent signaling are not fully understood. Recently, we reported that mast cell activation and anaphylaxis are negatively regulated by AMP-activated protein kinase (AMPK). However, little is known about the relationship between ERK1/2-mediated positive and the AMPK-mediated negative regulation of FcεRI signaling in mast cells. Objective: We investigated possible interactions between ERK1/2 and AMPK in the modulation of mast cell signaling and anaphylaxis. Methods: Wild-type or AMPKa22/2 mice, or bone marrow. derived mast cells obtained from these mice, were treated with either chemical agents or small interfering RNAs that modulated the activity or expression of ERK1/2 or AMPK to evaluate the functional interplay between ERK1/2 and AMPK in FcεRI-dependent signaling. Results: The ERK1/2 pathway inhibitor U0126 and the AMPK activator 5-aminoimidazole-4-carboxamide-1-b-4-ribofuranoside similarly inhibited FcεRI-mediated mast cell signals in vitro and anaphylaxis in vivo. ERK1/2-specific small interfering RNA also mimicked this effect on FcεRI signals. Moreover, AMPKa2 knockdown or deficiency led to increased FcεRI-mediated mast cell activation and anaphylaxis that were insensitive to U0126 or activator 5-aminoimidazole-4-carboxamide-1-b-4-ribofuranoside, suggesting that the suppression of FcεRI signals by the inhibition of the ERK1/2 pathway relies largely on AMPK activation. ERK1/2 controlled AMPK activity by regulating its subcellular translocation. Conclusions: ERK1/2 ablated the AMPK-dependent negative regulatory axis, thereby activating FcεRI signals in mast cells. (J Allergy Clin Immunol 2014;134:714-21.)

Microwave-assisted Approach for the Rapid Enzymatic Digestion of Rapeseed Meal

Ju-Fang Li,Fang Wei,Lu-Lu Guo,Gang-You Yuan,Feng-Hong Huang,Mu-Lan Jiang,Yuan-Di Zhao,Xu-Yan Dong,Guang-Ming Li,Hong Chen 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2

This study demonstrates the use of a new microwave-assisted approach for accelerating the enzymatic digestion of rapeseed meal. The effects of different microwave parameters, such as the time, temperature, and power level, on the degree of hydrolysis (DH) were investigated by using response surface methodology (RSM). The maximum predicted DH value (10.2%) was in good agreement with the value obtained experimentally using an alkaline protease, which was 12.57% under optimal conditions. In only 7 min, the microwave-assisted method achieved a DH value similar to that obtained by the conventional enzymatic digestion method (4 hr). Therefore,this new technique for rapid enzymatic digestion will improve the application of rapeseed meal in the preparation of protein hydrolysates for use in food and feed.

EGFR-TKI Combined with Pemetrexed versus EGFR-TKI Monotherapy in Advanced EGFR-Mutated NSCLC: A Prospective, Randomized, Exploratory Study

Weiguang Gu,Hua Zhang,Yiyu Lu,Minjing Li,Shuang Yang,Jianmiao Liang,Zhijian Ye,Zhihua Li,Minhong He,Xiaoliang Shi,Fei Wang,Dong You,Weiquan Gu,Weineng Feng 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose We aimed to evaluate whether the addition of pemetrexed is effective in improving progression-free survival (PFS) in epidermal growth factor receptor (EGFR)–mutated patients with or without concomitant alterations. Materials and Methods This multicenter clinical trial was conducted in China from June 15, 2018, to May 31, 2019. A total of 92 non–small cell lung cancer (NSCLC) patients harboring EGFR-sensitive mutations were included and divided into concomitant and non-concomitant groups. Patients in each group were randomly treated with EGFR–tyrosine kinase inhibitor (TKI) monotherapy or EGFR-TKI combined with pemetrexed in a ratio of 1:1. PFS was recorded as the primary endpoint. Results The overall median PFS of this cohort was 10.1 months. There were no significant differences in PFS between patients with and without concomitant and between patients received TKI monotherapy and TKI combined with pemetrexed (p=0.210 and p=0.085, respectively). Stratification analysis indicated that patients received TKI monotherapy had a significantly longer PFS in non-concomitant group than that in concomitant group (p=0.002). In concomitant group, patients received TKI combined with pemetrexed had a significantly longer PFS than patients received TKI monotherapy (p=0.013). Molecular dynamic analysis showed rapidly emerging EGFR T790M in patients received TKI monotherapy. EGFR mutation abundance decreased in patients received TKI combined chemotherapy, which supports better efficacy for a TKI combined chemotherapy as compared to TKI monotherapy. A good correlation between therapeutic efficacy and a change in circulating tumor DNA (ctDNA) status was found in 66% of patients, supporting the guiding role of ctDNA minimal residual disease (MRD) in NSCLC treatment. Conclusion EGFR-TKI monotherapy is applicable to EGFR-sensitive patients without concomitant alterations, while a TKI combined chemotherapy is applicable to EGFR-sensitive patients with concomitant alterations. CtDNA MRD may be a potential biomarker for predicting therapeutic efficacy.

Performance of water-jet pump under acceleration

Wu Xian-Fang,Li Ming-Hui,Liu Houlin,Tan Minggao,Lu You-Dong 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

The instantaneous acceleration affects the performance of the water-jet pump obviously. Here, based on the user-defined function, the method to simulate the inner flow in water-jet pumps under acceleration conditions was established. The effects of two different acceleration modes (linear acceleration and exponential acceleration) and three kinds of different acceleration time (0.5s, 1s and 2s) on the performance of the water-jet pump were analyzed. The results show that the thrust and the pressure pulsation under exponential acceleration are lower than that under linear acceleration at the same time; the vapor volume fraction in the impeller under linear acceleration is 27.3% higher than that under exponential acceleration. As the acceleration time increases, the thrust gradually increases and the pressure pulsation amplitude at the impeller inlet and outlet gradually decreases, while the law of pressure pulsation is the opposite at the diffuser outlet. The main frequency of pressure pulsation at the impeller outlet is different under different acceleration time. The research results can provide some reference for the optimal design of water-jet pumps.