Roles of NMDA NR2B Subtype Receptor in Prefrontal Long-Term Potentiation and Contextual Fear Memory

Zhao, Ming-Gao,Toyoda, Hiroki,Lee, Yong-Seok,Wu, Long-Jun,Ko, Shanelle W.,Zhang, Xue-Han,Jia, Yongheng,Shum, Fanny,Xu, Hui,Li, Bao-Ming,Kaang, Bong-Kiun,Zhuo, Min Elsevier 2005 Neuron Vol.47 No.6

<P><B>Summary</B></P><P>Cortical plasticity is thought to be important for the establishment, consolidation, and retrieval of permanent memory. Hippocampal long-term potentiation (LTP), a cellular mechanism of learning and memory, requires the activation of glutamate N-methyl-D-aspartate (NMDA) receptors. In particular, it has been suggested that NR2A-containing NMDA receptors are involved in LTP induction, whereas NR2B-containing receptors are involved in LTD induction in the hippocampus. However, LTP in the prefrontal cortex is less well characterized than in the hippocampus. Here we report that the activation of the NR2B and NR2A subunits of the NMDA receptor is critical for the induction of cingulate LTP, regardless of the induction protocol. Furthermore, pharmacological or genetic blockade of the NR2B subunit in the cingulate cortex impaired the formation of early contextual fear memory. Our results demonstrate that the NR2B subunit of the NMDA receptor in the prefrontal cortex is critically involved in both LTP and contextual memory.</P>

Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau

Hui-bin Lu,Xiao-feng Xue,Dorji Phurbu,Peng Xing,Qing-long Wu 한국미생물학회 2018 The journal of microbiology Vol.56 No.11

Two Gram-stain negative halophilic strains, designated as LM2T and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase- positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2T and LM4 belong to the genus Roseovarius, with Roseovarius tolerans EL-172T (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084T (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were C18:1 ω7c/C18:1 ω6c, C16:0, C19:0 cyclo ω8c, and 11-methyl C18:1 ω7c. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2T and LM4, 24.9% relatedness between LM2T and R. tolerans EL-172T, and 36.3% relatedness between LM4 and R. tolerans EL-172T. Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2T and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius. It was evident that LM2T and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2T (= CGMCC 1.16230T = KCTC 62028T).

Influence of Soil Erosion Thickness on Soybean Yield and Coupling Mode of Water and Fertilizers of Black Soil in Northeast China

Hui Liu,Yongxia Wei,Long Wang,Xian Wu 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.3

Soil erosion is one of the most widespread ecological problems with the most serious damage worldwide. In the present study, the influence of soil erosion thickness on soybean yield of black soil in Northeast China is studied. With the pot experiment as the method, this study investigates the influence by peeling the surface black soil layer at 0, 5, 10, 20, 25, and 30 cm to simulate different soil erosion thicknesses, establishes a quantitative model of soybean yield to explore the response of soybean yield to soil erosion thickness. The 4-D-optimum orthogonal design (416-A) is adopted to conduct the coupling experiment of water and fertilizers under erosion thicknesses of 0 and 15 cm so that the coupling modes of water and fertilizers under the two soil erosion thicknesses are established. The results show that soil erosion thickness has a significant influence on soybean growth, that is, with the increase in the soil erosion thickness, soybean plant height, pods and grains per plant all reduce, and soybean yield decreases in a Z-shaped curve, while yield reduction rate increases in a S-shaped curve, respectively. Water, along with N, P, and K fertilizers, affect soybean yield, but the intensity varies with soil erosion thickness. Under the soil erosion thickness of 0 cm, the order is: W>P>N>K. While under the soil erosion thickness of 15 cm, the order is: N>W>P>K. With the increase in soil erosion thickness and the decrease in soil fertility, the effect of irrigation reduces, whereas that of fertilization increases. The research results can provide the theoretical basis for soil erosion prevention and control in the black soil region.

Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau

Hui-bin Lu,Peng Xing,Lei Zhai,Dorji Phurbu,Qian Tang,Qing-long Wu 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

Strains pyc13T and ZGT13 were isolated from Lake Pengyan and Lake Zigetang on Tibetan Plateau, respectively. Both strains were Gram-negative, catalase- and oxidase-positive, aerobic, rod-shaped, nonmotile, and nonflagellated bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains pyc13T and ZGT13 belong to the genus Halomonas, with Halomonas alkalicola 56-L4-10aEnT as their closest neighbor, showing 97.4% 16S rRNA gene sequence similarity. The predominant respiratory quinone of both strains was Q-9, with Q-8 as a minor component. The major fatty acids of both strains were C18:1 ω6c/C18:1 ω7c, C16:1 ω6c/C16:1 ω7c, C16:0, and C12:0 3OH. The polar lipids of both strains consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, glycolipid, phospholipids of unknown structure containing glucosamine, and unidentified phospholipids. The DNA G + C content of pyc13T and ZGT13 were 62.6 and 63.4 mol%, respectively. The DNA-DNA hybridization values of strain pyc13T were 34, 41, 61, 35, and 35% with the reference strains H. alkalicola 56-L4-10aEnT, H. sediminicola CPS11T, H. mongoliensis Z-7009T, H. ventosae Al12T, and H. fontilapidosi 5CRT, respectively. Phenotypic, biochemical, genotypic, and DNA-DNA hybridization data showed that strains pyc13T and ZGT13 represent a new species within the genus Halomonas, for which the name H. tibetensis sp. nov. is proposed. The type strain is pyc13T (= CGMCC 1.15949T = KCTC 52660T).

Performance and applications of flow-guided sieve trays for distillation of highly viscous mixtures

Hui Liu,Qun Shen Li,Chun Ying Song,Hai Long Wu,Yi Qiu Qian 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6

With a particular focus on the distillation of highly viscous or self-polymerized mixtures, this study reports the hydrodynamic and mass transfer performance of two flow-guided sieve trays, including their pressure drop, entrainment, weeping and tray efficiency, obtained experimentally with an air-water/oxygen system in a Φ600 mm plexiglass column. The results show that the 8 mm hole flow-guided sieve tray tested shows better characteristics than the 7 mm flow-guided hole tray in terms of pressure drop and mass transfer. Then we present practical industrial examples of applications of the flow guided trays for distillation of viscous mixtures, i.e., the separation of vinyl acetate (VAC) from a polyvinyl acetate (PVAC) polymer solution with dynamic viscosity μ=50,000 mPas, the separation of highly unsaturated C5 mixtures by extractive distillation, and the distillation of thick, condensed and highly viscous fermentation mixtures made from fermented mash. It is demonstrated that flow-guided sieve trays with relatively large holes are an excellent candidate for distillation of mixtures with suspended solids, or concentrated/self-polymerized polymer solutions.

Ultrasonic Vibration Assisted Cutting of Nomex Honeycomb Core Materials

Dao‑Hui Xiang,Bang‑Fu Wu,Yun‑Long Yao,Bo Zhao,Jin‑Yuan Tang 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.1

Nomex honeycomb core materials have been widely used in the aviation industry due to their special structure and performance. Conventional high-speed machining have resulted in the poor machinability of the honeycomb core so that the ultrasonic machining technology was applied. The kinematic characteristics in the ultrasonic vibration assisted cutting process were analyzed according to the movement of the sharp tool. Based on slide effect, a cutting force model was proposed to study the relationship between cutting parameters and cutting force. Ultrasonic vibration assisted cutting and ordinary cutting tests of Nomex honeycomb core material were conducted by considering feed rate, the inclined angle and the deflected angle. Besides, the effects of cutting parameters on machined surface quality of honeycomb core wall were studied. The test results show that slide effect caused by ultrasonic vibrations can reduce cutting resistance compared with ordinary cutting. The developed cutting force model can be applied to evaluated the cutting force in the ultrasonic vibration assisted cutting of Nomex honeycomb core material. The inclined angle has a great influence on the cutting force during ultrasonic vibration assisted cutting. High-speed reciprocating sliding action can effectively cut aramid fibers so that burrs and tearing defects of the incision have been greatly improved under condition of ultrasonic vibration assisted cutting.

Heavy concrete shielding properties for carbon therapy

Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm³ to 4.14 g/cm³, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm³, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm³ with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.

H₂O₂ Inhibits Proliferation and Mediates Suppression of Migration via DLC1/RhoA Signaling in Cancer Cells

Ma, Long,Zhu, Wen-Zhen,Liu, Ting-Ting,Fu, Hui-Ling,Liu, Zhao-Jun,Yang, Bing-Wu,Song, Tai-Yu,Li, Guo-Rong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Background: RhoGTPase-activating proteins (RhoGAPs) regulate RhoGTPases in cells, but whether individual reactive oxygen species (ROS) regulate RhoGAPs is unknown. Our previous published papers have shown that deleted in liver cancer 1 (DLC1) inhibits cancer cell migration by its RhoGAP activity. The present study was designed to explore the role of $H_2O_2$ in regulation of DLC1. Materials and Methods: We treated cells with $H_2O_2$ for 24h and phenotypic changes were analyzed by MTT, RT-PCR, Western blotting, immunofluorescence staining and wound healing assays. Results: $H_2O_2$ downregulated cyclin D1 and cyclin E to inhibit proliferation, and upregulated BAX to induce apoptosis in MCF-7 cells. Compared with non-tumorigenic cells, $H_2O_2$ increased expression of DLC1 and reduced activity of RhoA in cancer cells. Stress fiber production and migration were also suppressed by $H_2O_2$ in MDA-MB-231 cells. Conclusions: Our study suggests that $H_2O_2$ inhibits proliferation through modulation of cell cycle and apoptosis-related genes, and inhibits migration by decreasing stress fibers via DLC1/RhoA signaling.

FEM analysis of the modular prefabricated steel-concrete composite beam-column internal joint under reciprocating action

Cheng Long Wu,Jian Cheng Kan,Qi Hui Wang,Ji Ming Liu,Zun Qiang Li 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.1

Prefabricated steel-concrete composite structure has the advantages of large bearing capacity, reliable connection, and good durability, which is of great significance to the development of building industrialization. In this paper, a finite element model (FEM) of the modular prefabricated SRC column-steel beam composite joint is established and verified its accuracy through ABAQUS. The effects of various parameter variables on the hysteresis curve, skeleton curve, ductility, energy consumption, and performance deterioration are investigated. The results show that as the axial compression ratio increases, the joint bearing capacity first increases and then decreases, the strength and stiffness have relatively stable degradation characteristics. However, the hysteresis curve has a certain “pinch” phenomenon, which reduces the energy consumption performance. Different column end bolts margin and apertures have little effect on the bearing capacity, strength and stiffness degradation performance, but affect energy consumption and ductility. The width-to-thickness ratio of the square steel tube has a greater impact on the bearing capacity, energy consumption and ductility, and has less impact on the strength and stiffness degradation performance. As the thickness of the joint cover plate increases, the bearing capacity, energy consumption and initial stiffness of the joint gradually increase, the ductility first increases and then decreases.

Enhancement of Polysialic Acid Yield by Reducing Initial Phosphate and Feeding Ammonia Water to Escherichia coli CCTCC M208088

Jian-Rong Wu,Jin-Long Liu,Xiao-Bei Zhan,Chi-Chung Lin,Hui Zhao 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4

Polysialic acid (PSA) is a capsular polysaccharide obtained from aerobic fermentation with Escherichia coli. To enhance PSA production and eliminate the influence of phosphate on the PSA purification process, a lower level of initial phosphate was adopted with pH control. The resulting PSA yield reached 4.1 g/L in fed-batch fermentation with 2.5 g/L K2HPO4 and E. coli strain CCTCC M208088. In addition, an ammonia water (NH4OH) feeding strategy to control the pH at 6.4 was developed resulting in PSA production that reached as high as 5.2 g/L. NMR spectra confirmed the purified biopolymer as a α-2,8linked PSA, identical to the published NMR spectra, with a molecular weight in the range of 16 ~ 50 kDa.