Fabrication and Characterization of Moisture Slow-releasing Embroidered Electrode and ECG Monitoring Belt

Liyan Liu,Meijing Ma,Dongyu Tang,Yao Hu,Hao Liu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.12

The microenvironmental humidity at the interface between the bioelectrical electrode and the human skin canaffect the quality of the bioelectrical signal collected by the electrode. This paper utilized superabsorbent polyacrylic fiber asthe base material to fabricate a novel moisture slow-releasing embroidered electrode. The moisture-locking capacity ofpolyester non-woven fabric (PENWF) substrate, polyacrylic non-woven fabric (PANWF) substrate, polyester-basedelectrode (PE-E) and polyacrylic-based electrode (PA-E) were compared in this paper. The non-woven fabric withpolyacrylic fiber owned excellent moisture-locking capacity, so it could realize the slow-releasing of moisture and provide asuitable wet environment for the dry bioelectric electrode to collect ECG signals. With the extension of time, the amplitude ofthe ECG signal collected by PA-E did not change much, and only decreased by 17.1 % after evaporating for 10 h; but afterevaporating for 10 h, the amplitude of the ECG signal collected by the PE-E decreased by 48.9 %. In addition, 5 mm, 10 mm,and 15 mm thick polyurethane sponge (PUS) filling materials were used to make the ECG monitoring belts, and their signalto-noise ratios were analyzed under different states of static, swinging arms and walking. The 5 mm thick elastic fillermaterial has the highest signal-to-noise ratio among the three thicknesses. It could collect ECG signals stably under swingarm and walking at a constant speed, and the signal-to-noise ratio (SNR) were 25.393 dB and 30.086 dB respectively. Thepolyurethane sponge filling materials with a thickness of 5 mm provided an appropriate pre-stress for the ECG signaldynamic measurement, which could provide a reference parameter for the production of smart ECG garment in the future.

Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi

Defu Wang,Liyan Cui,Li Zhang,Zhennan Ma,Yanbing Niu 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.2

Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soy- bean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross- family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly tech- niques to carry out SMV full-length genome amplifi- cation from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes a polyprotein consisting of 3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and ami- no acid sequence identities of 97.03% and 98.50%, re- spectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected plant tissue. This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infec- tious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli, thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms.

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

Defu Wang,Liyan Cui,Yanni Pei,Zhennan Ma,Shaofei Shen,Dandan Long,Lingyu Li,Yanbing Niu 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5

Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCVSX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCVSX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for “A” and “U” at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease.

Occurrence of ethyl carbamate in three types of Chinese wines and its possible reasons

Zhengfu Wang,Lili Yang,Liyan Ma,Xingzhi Liu,Jingming Li 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A total of 75 wines including 30 white wines, 31 red wines, and 14 sparkling wines wereobtained from several regions in China (Sinkiang, Tonghua, Huailai, Yantai, Changli, Shanxi, Gansu, andNingxia). Ethyl carbamate (EC) was detected by gas chromatography mass spectrometry. The ECconcentration ranged from less than 1.16 to 38.56 μg/L, and the concentrations in 17 wines exceededthe U.S. limit for table wines (15 μg/L). The concentrations of EC increased in the order of white, red,and sparkling wines with the corresponding mean concentrations of 6.12, 9.22, and 14.03 μg/L. Therelationship between EC concentration and wine type suggested that EC concentrations in wines mightbe affected by vinification patterns, most likely due to the difference between EC precursors indifferent vinification processes. This work provides a novel clue for EC contamination in different wines.

Occurrence of ethyl carbamate in three types of Chinese wines and its possible reasons

Fu, Zheng,Yang, Lili,Ma, Liyan,Liu, Xingzhi,Li, Jingming 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A total of 75 wines including 30 white wines, 31 red wines, and 14 sparkling wines were obtained from several regions in China (Sinkiang, Tonghua, Huailai, Yantai, Changli, Shanxi, Gansu, and Ningxia). Ethyl carbamate (EC) was detected by gas chromatography mass spectrometry. The EC concentration ranged from less than 1.16 to $38.56{\mu}g/L$, and the concentrations in 17 wines exceeded the U.S. limit for table wines ($15{\mu}g/L$). The concentrations of EC increased in the order of white, red, and sparkling wines with the corresponding mean concentrations of 6.12, 9.22, and $14.03{\mu}g/L$. The relationship between EC concentration and wine type suggested that EC concentrations in wines might be affected by vinification patterns, most likely due to the difference between EC precursors in different vinification processes. This work provides a novel clue for EC contamination in different wines.

Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

Multicenter, randomized study of genetically modified recombinant human interleukin-11 to prevent chemotherapy-induced thrombocytopenia in cancer patients receiving chemotherapy

Wu, Shikai,Zhang, Yang,Xu, Liyan,Dai, Yun,Teng, Yuee,Ma, Shanshan,Ho, Seong-Hyun,Kim, Jong-Mook,Yu, Seung Shin,Kim, Sunyoung,Song, Santai Springer-Verlag 2012 Supportive care in cancer Vol.20 No.8

The Effect of Dipping Pretreatment on Ochratoxin A Accumulation in Sultanas and Currants

Xiaoxu Zhang,Jingming Li,Dong Wang,Saisai Feng,Liyan Ma 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

The dipping pretreatment on the occurrence of ochratoxin A (OTA) in sultanas and currants was investigated. Grape samples were divided into two groups before a drying process. One group was dipped with potassium carbonate-ethyl oleate, and the other group was left without treatment (the control). OTA were detected using solid-phase extraction clean-up and a high-performance liquid chromatography fluorescence detector. Results showed that OTA content was below the limit of detection in the fresh grapes of both varieties. OTA were not detected in dried vine fruits before storage. However, a real naturally environmental storage revealed that the dipping pretreated samples were contaminated with OTA earlier and to a greater extent than the control. The OTA concentration in the treated and control samples after one-year storage was 0.22 and 0.19 μg/kg for sultanas and 0.34 and 0.21 μg/kg for currants, respectively. These results indicated that dipping pretreatment might increase the toxin contamination and safety risk.