Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng

Sun, Hang,Liu, Fangbing,Sun, Liwei,Liu, Jianzeng,Wang, Manying,Chen, Xuenan,Xu, Xiaohao,Ma, Rui,Feng, Kai,Jiang, Rui The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2

Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

Facile microwave-assisted solvothermal synthesis of rod-like aluminum terephthalate [MIL-53(Al)] for CO2 adsorption

Liwei Sun,Meilin Yin,Zhen Li,Shaokun Tang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

To alleviate environment issues derived from CO2 emission, developing effective and economical methodsfor CO2 capture has attracted much attention for decades. Metal-organic frameworks possess highporosity and stability, which is favorable to the adsorption of small molecules. Herein, an aluminumbasedMOF, aluminum terephthalate [MIL-53(Al)], was synthesized with H2O and N,N0-dimethylformamide as co-solvent via microwave-assisted method for CO2 adsorption. The parametersincluding pre-mixing mode, microwave temperature, microwave power and reaction time were variedto study their influences on crystal structure, morphology and CO2 adsorption capacity of MW-MIL-53(Al). Wherein, MW-MIL-53(Al) prepared with pre-mixing mode of stirring at 130 C, 200 W and 3 h presentshigh crystallinity and rod-like morphology. The synthesized MW-MIL-53(Al) possesses relativelyhigh BET surface area (961 m2/g), pore volume (0.46 cm3/g) and thermal stability (up to 480 C), andits CO2 adsorption capacity is 2.16 mmol CO2/g adsorbent under 25 C and 0.1 MPa, which is higher thanCS-MIL-53(Al) synthesized via conventional solvothermal method at 130 C for 48 h.

Synthesis of bi-functionalized ionic liquid - mesoporous alumina composite material and its CO2 capture capacity

Liwei Sun,Shaokun Tang 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.10

Bi-functionalized ionic liquid (IL) - mesoporous alumina (MA) composite material was synthesized and used for CO2 capture. Ordered mesoporous alumina was synthesized by self-assembly method with aluminum isopropoxide as aluminum source. Then bi-functionalized ionic liquid 1-methoxyethyl-3-methyl imidazole glycinate ([MOEmim][Gly]) was immobilized on mesoporous alumina by ultrasonic-assisted impregnation method. Ordered mesostructure of alumina keeps well in the composite material. Compared with bi-functionalized ionic liquid, thermal stability of the composite material greatly improved. Finally, CO2 capture capacity of IL-MA composite material was studied under different temperatures. On the basis of both capture capacity and capture rate, 40 oC is the optimal temperature. The capture capacity is 1.42mol·mol IL−1 - equivalent to 144mg·g sorbent−1, which is higher than IL or MA alone. Furthermore, the capture capacity of composite material almost maintains constant after eight capture-regeneration cycles.

Research on Influence of Multimedia Network Teaching Platform and Effective Interaction on Physical Education Curriculum Based on Smart Classroom

Liwei SUN 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.11

With the development of computer information technology and multimedia Internet technology, human beings have entered a new era of knowledge and network. Education information construction is an important way for the modernization of education, in this paper, the author research on influence of multimedia network teaching platform and effective interaction on physical education curriculum. For the necessity of multimedia and network assisted instruction, 68.57% teachers think that multimedia teaching is very important, and it is necessary means of physical education teaching. Multimedia network teaching platform can also integrate the latest sports information resources, students can watch all kinds of large-scale sports events by online video.

Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng

Hang Sun,Fangbing Liu,Liwei Sun,Jianzeng Liu,Manying Wang,Xuenan Chen,Xiaohao Xu,Rui Ma,Kai Feng,Rui Jiang 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2

Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

Nitrogen removal, nitrous oxide emission and microbial community in sequencing batch and continuous-flow intermittent aeration processes

Yuepeng Sun,Liwei Xin,Guangxue Wu,Yuntao Guan 대한환경공학회 2019 Environmental Engineering Research Vol.24 No.1

Nitrogen removal, nitrous oxide (N₂O) emission and microbial community in sequencing batch and continuous-flow intermittent aeration processes were investigated. Two sequencing batch reactors (SBRs) and two continuous-flow multiple anoxic and aerobic reactors (CMRs) were operated under high dissolved oxygen (DO) (SBR-H and CMR-H) and low DO (SBR-L and CMR-L) concentrations, respectively. Nitrogen removal was enhanced under CMR and low DO conditions (CMR-L). The highest total inorganic nitrogen removal efficiency of 91.5% was achieved. Higher nitrifying and denitrifying activities in SBRs were observed. CMRs possessed higher N₂O emission factors during nitrification in the presence of organics, with the highest N₂O emission factor of 60.7% in CMR-L. SBR and low DO conditions promoted N₂O emission during denitrification. CMR systems had higher microbial diversity. Candidatus Accumulibacter, Nitrosomonadaceae and putative denitrifiers (N₂O reducers and producers) were responsible for N₂O emission.

Combination of supercritical fluid elution and resin adsorption for removal of procymidone from ginseng extracts

Guangtao Li,Liwei Sun,Shaokun Tang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.4

We propose a new method of resin adsorption (RA) coupled with supercritical fluid elution (SFE) for removal of pesticide residue and recovery of ginsenosides from ginseng extracts. D-101-1 resin was selected as the proper adsorption resin, acetone-n-hexane (4 : 6, v : v) served as the modifier with the flow rate of 1.5mL/min during supercritical CO2 elution of procymidone at 25MPa, 55 oC for 2 h, and absolute ethanol as the modifier at a flow rate of 1mL/min for supercritical CO2 elution of ginsenosides at 20MPa, 60 oC and 1 h. The results showed that the content of procymidone in the final products was only 0.0089mg/kg. Meanwhile, the recovery rate of ginsenosides reached up to 92.5%. RA-SFE procedure provides an efficient approach to remove pesticide residue traces with little loss of active ingredients. The used resin can be recycled without any additional regeneration.

Efficient Constitutive Expression of Cellulolytic Enzymes in Penicillium oxalicum for Improved Efficiency of Lignocellulose Degradation

( Pankajkumar Ramdas Waghmare ),( Pratima Pankajkumar Waghmare ),( Liwei Gao ),( Wan Sun ),( Yuqi Qin ),( Guodong Liu ),( Yinbo Qu ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.5

Efficient cellulolytic enzyme production is important for the development of lignocellulose-degrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

Trisodium citrate-assisted synthesis of BiOBr nanostructure catalyst for efficient activity under visible light

Weiming Zhou,Yifan Jiang,Shichang Sun,Mingxin Zhang,Ibrahim Lawan,Gerard Franklyn Fernando,Liwei Wang,Zhanhui Yuan 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.2

A simple one step wet-chemical method assisted by trisodium citrate was employed in the synthesis of BiOBr applied as a photocatalyst. Photocatalytic activity of the BiOBr was investigated for the degradation of Rhodamine B (RhB) dye under visible light irradiation (>420 nm). The results indicated that trisodium citrate is more favorable to the formation of hierarchical architectures and reduces the particle size of BiOBr photocatalyst. BiOBr with hierarchical architectures exhibiting significantly higher catalytic activity than that with ordinary nanostructure. The significant improvement could be attributed to the high specific surface area (24.14m2·g1), average pore sizes (34.09 nm) and average pore volume (0.24 cm3·g1).

Feasibility of Three-Dimensional Balanced Steady-State Free Precession Cine Magnetic Resonance Imaging Combined with an Image Denoising Technique to Evaluate Cardiac Function in Children with Repaired Tetralogy of Fallot

Peng YaFeng,Su XinYu,Hu LiWei,Wang Qian,Ouyang RongZhen,Sun AiMin,Guo Chen,Yao XiaoFen,Zhang Yong,Wang LiJia,Zhong YuMin 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.9

Objective: To investigate the feasibility of cine three-dimensional (3D) balanced steady-state free precession (b-SSFP) imaging combined with a non-local means (NLM) algorithm for image denoising in evaluating cardiac function in children with repaired tetralogy of Fallot (rTOF). Materials and Methods: Thirty-five patients with rTOF (mean age, 12 years; range, 7–18 years) were enrolled to undergo cardiac cine image acquisition, including two-dimensional (2D) b-SSFP, 3D b-SSFP, and 3D b-SSFP combined with NLM. End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and ejection fraction (EF) of the two ventricles were measured and indexed by body surface index. Acquisition time and image quality were recorded and compared among the three imaging sequences. Results: 3D b-SSFP with denoising vs. 2D b-SSFP had high correlation coefficients for EDV, ESV, SV, and EF of the left (0.959– 0.991; p < 0.001) as well as right (0.755–0.965; p < 0.001) ventricular metrics. The image acquisition time ± standard deviation (SD) was 25.1 ± 2.4 seconds for 3D b-SSFP compared with 277.6 ± 0.7 seconds for 2D b-SSFP, indicating a significantly shorter time with the 3D than the 2D sequence (p < 0.001). Image quality score was better with 3D b-SSFP combined with denoising than with 3D b-SSFP (mean ± SD, 3.8 ± 0.6 vs. 3.5 ± 0.6; p = 0.005). Signal-to-noise ratios for blood and myocardium as well as contrast between blood and myocardium were higher for 3D b-SSFP combined with denoising than for 3D b-SSFP (p < 0.05 for all but septal myocardium). Conclusion: The 3D b-SSFP sequence can significantly reduce acquisition time compared to the 2D b-SSFP sequence for cine imaging in the evaluation of ventricular function in children with rTOF, and its quality can be further improved by combining it with an NLM denoising method.