Bioinspired Fabric with Superhydrophilicity and Superoleophobicity for Efficient Oil/Water Separation

Liping Liang,Chenlu Wang,Hongfang Wang,Haihua Zhan,Xu Meng 한국섬유공학회 2018 Fibers and polymers Vol.19 No.9

The cotton fabric was modified with dopamine methacrylamide (DMA) based on mussel-inspired reaction and polymerized with zwitterionic sulfobetaine methacrylate (SBMA) through free radical polymerization reaction. The poly(DMA-SBMA) contained not only key chemical constituents of dopamine hydrochloride, which strongly adsorbed to fabric substrates, but also hydrophilic groups, providing a hydrophilic surface for fabric due to its strong interaction with water via electrostatic interactions. The chemical structure, surface topography, and surface wettability of the fabric were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and contact angle experiments, respectively. The results showed that the water contact angle (WCA) of the treated fabric was ~0 o, whereas the underwater oil contact angle (OCA) was ~161 o, as compared to ~25 o for the control one. It is expected that as-prepared fabrics could be applied in oil/water separation due to such special superhydrophilicity and superoleophobicity.

Large-scale production of foreign proteins via the novel plant transient expression system in Pisum sativum L

Liang Li,Xiufeng Wang,Liping Yang,Yajun Fan,Xiaojuan Zhu,Xingzhi Wang 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.4

Transient expression of foreign genes by Agrobacterium infiltration is a versatile technique that can be used as a rapid tool for functional protein production in plants. A reproducible protocol of large-scale production of foreign proteins via the novel plant transient expression system in Pisum sativum L. was established in our study. Non-detached plants from soil-independent culture were used as the target organ, and vacuum infiltrating mediated by Agrobacterium tumefaciens harboring green fluorescent protein (GFP) gene was performed. Step-by-step optimization was performed and showed that the quality of plant material as well as agro-infiltration conditions were the major factors influencing the gene expression. Monitoring the transient GFP expression daily, the highest expression level was achieved on the 8th day post-infiltration. Evidence of anti-acidic fibroblast growth factorsingle chain variable fragment (anti-aFGF-scFv) gene expression in pea seedling was also achieved using agromediated vacuum infiltration system. Our work proves that the system is suitable for the largescale production of pharmaceutical proteins. The in planta infiltration system described here provides a powerful tool to explore easily gene expression in Pisum sativum L. avoiding tissue culture steps and the labor-intensive generation of transgenic plants.

Composite action of concrete-filled double circular steel tubular stub columns

Liping Wang,Xing-xing Cao,Fa-Xing Ding,Liang Luo,Yi Sun,Xue-mei Liu,Hui-lin Su 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.1

This paper presents a combined numerical, experimental, and theoretical study on the behavior of the concrete-filled double circular steel tubular (CFDT) stub columns under axial compressive loading. Four groups of stub column specimens were tested in this study to find out the effects of the concrete strength, steel ratio and diameter ratio on the mechanical behavior of CFDT stub columns. Nonlinear finite element (FE) models were also established to study the stresses of different components in the CFDT stub columns. The change of axial and transverse stresses in the internal and external steel tubes, as well as the change of axial stress in the concrete sandwich and concrete core, respectively, was thoroughly investigated for different CFDT stub columns with the same steel ratio. The influence of inner-to-outer diameter ratio and steel ratio on the ultimate bearing capacity of CFDT stub columns was identified, and a reasonable section configuration with proper inner-to-outer diameter ratio and steel ratio was proposed. Furthermore, a practical formula for predicting the ultimate bearing capacity was proposed based on the ultimate equilibrium principle. The predicted results showed satisfactory agreement with both experimental and numerical results, indicating that the proposed formula is applicable for design purposes.

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liping Xie,Zhen Liang,Shiqi Li,Xin Xu,Xianglai Xu,Xiao Wang,Jian Wu,Yi Zhu,Zhenghui Hu,Yiwei Lin,Yeqing Mao,Hong Chen,Jindan Luo,Ben Liu,Xiangyi Zheng 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3 -UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3 -UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liang, Zhen,Li, Shiqi,Xu, Xin,Xu, Xianglai,Wang, Xiao,Wu, Jian,Zhu, Yi,Hu, Zhenghui,Lin, Yiwei,Mao, Yeqing,Chen, Hong,Luo, Jindan,Liu, Ben,Zheng, Xiangyi,Xie, Liping Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

NEW PRODUCT INTRODUCTION VIA SOCIAL MEDIA INFLUENCER

Zhenhao Li,Liping Liang 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07

Firms are increasingly using social media influencers to promote their products. We develop a two-period model to investigate a firm’s strategy for introducing a product via an influencer, where there is uncertainty in the influencer-product match. In the second period, the influencer exerts an effort to promote the product to her followers, who can spread the product information to non-followers via word-of-mouth (WOM). In the second period, the firm sells to the non-followers. We show that the firm’s pricing, production, and commission contract decisions depend on the influencer’s incentive-independent excess payoff from the promotion and on the difference between the WOM effect of followers who do or do not make a purchase rather than the WOM effect of each group. As the influencer’s incentive-independent payoff increase, the firm will increase (decrease) commissison rate and commission rate when the followers’ sensitivity to product price is relatively low (high) compared with that to the influencer’s effort. As the marginal WOM benefit of the first-period sales increases, the firm tends to reduce his unit net profit from sales. The influencer with a medium-sized follower base receives the highest commission rate and exerts the largest promotion effort. While the followers of influencers with a medium-sized follower base may pay the highest price. We also show that (i) there exists a threshold for the probability of match, above which the firm faces zero demand in the first period if an influencer-product mismatch occurs; and (ii) the firm may charge followers a lower price than non-followers, even though followers are less sensitive to price than non-followers. Finally, regarding influencer selection, we find that the firm may not be better off employing an influencer with a larger follower base.

A facile approach to preparing palladium nanoparticles-embedded polyvinylpyrrolidone (PVP) heterogeneous hybrid nanofibers mats by electrospinning

Jie Bai,Liping Guo,Haiou Liang,Tong Xu,Chunping Li,Qingrun Meng,Huan Liu,Yarong Huang 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.12

Well-dispersed palladium nanoparticles (Pd NPs) were prepared under the condition that trisodium citrate was the reduction agent and polyvinylpyrrolidone (PVP) was the stabilizing agent via sol-gel process. By making good use of the advanced electrospinning technology we obtained Pd NPs/PVP composite nanofibers films. Optical properties were examined by UV-visible absorbance spectra (UV-vis) and Fourier transform infrared spectroscopy (FTIR). The morphology and distribution of Pd NPs in/on PVP matrix were characterized by scanning electron microscope (SEM)and transmission electron microscope (TEM). The results showed that Pd NPs presented as spherical spots and distributed in/on PVP nanofibers uniformly; their diameter was 4-10 nm and decreased with the increase of PVP. The as-prepared Pd NPs/PVP hybrid mats possess catalytic activity, stability and reusability, as verified by performance in Heck reaction.

Evaluation of Dry Matter Intake and Average Daily Gain Predicted by the Cornell Net Carbohydrate and Protein System in Crossbred Growing Bulls Kept in a Traditionally Confined Feeding System in China

Du, Jinping,Liang, Yi,Xin, Hangshu,Xue, Feng,Zhao, Jinshi,Ren, Liping,Meng, Qingxiang Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.11

Two separate animal trials were conducted to evaluate the coincidence of dry matter intake (DMI) and average daily gain (ADG) predicted by the Cornell Net Carbohydrate and Protein System (CNCPS) and observed actually in crossbred growing bulls kept in a traditionally confined feeding system in China. In Trial 1, 45 growing Simmental${\times}$Mongolia crossbred F1 bulls were assigned to three treatments (T1-3) with 15 animals in each treatment. Trial 2 was conducted with 60 Limousin${\times}$Fuzhou crossbred F2 bulls allocated to 4 treatments (t1-4). All of the animals were confined in individual stalls. DMI and ADG for each bull were measured as a mean of each treatment. All of the data about animals, environment, management and feeds required by the CNCPS model were collected, and model predictions were generated for animals on each treatment. Subsequently, model-predicted DMI and ADG were compared with the actually recorded results. In the three treatments in Trial 1, 93.3, 80.0 and 73.3% of points fell within the range from -0.4 to 0.4 kg/d for DMI mean bias; similarly, in the four treatments in Trial 2, about 86.7, 73.3, 73.3 and 80.0% of points fell within the same range. These results indicate that the CNCPS model can accurately predict DMI of crossbred bulls in the traditionally confined feeding system in China. There were no significant differences between predicted and observed ADG for T1 (p = 0.06) and T2 (p = 0.09) in Trial 1, and for t1 (p = 0.07), t2 (p = 0.14) and t4 (p = 0.83) in Trial 2. However, significant differences between predicted and observed ADG values were observed for T3 in Trial 1 (p<0.01) and for t3 in Trial 2 (p = 0.04). By regression analysis, a statistically different value of intercept from zero for the regression equation of DMI (p<0.01) or an identical value of ADG (p = 0.06) were obtained, whereas the slopes were significantly different (p<0.01) from unity for both DMI and ADG. Additionally, small root mean square error (RMSE) values were obtained for the unbiased estimator of the two variances (DMI and ADG). Thus, the present results indicated that the CNCPS model can give acceptable estimates of DMI and ADG of crossbred growing bulls kept in a traditionally confined feeding system in China.

Construction of Block-LDPC Codes based on Quadratic Permutation Polynomials

Wu Guan,Liping Liang 한국통신학회 2015 Journal of communications and networks Vol.17 No.2

A new block low-density parity-check (Block-LDPC) code based on quadratic permutation polynomials (QPPs) is proposed. The parity-check matrix of the Block-LDPC code is composed of a group of permutation submatrices that correspond to QPPs. The scheme provides a large range of implementable LDPC codes. Indeed, the most popular quasi-cyclic LDPC (QC-LDPC) codes are just a subset of this scheme. Simulation results indicate that the proposed scheme can offer similar error performance and implementation complexity as the popular QC-LDPC codes.

Ardipusilloside-I stimulates gastrointestinal motility and phosphorylation of smooth muscle myosin by myosin light chain kinase

Xu, Zhili,Liang, Hanye,Zhang, Mingbo,Tao, Xiaojun,Dou, Deqiang,Hu, Liping,Kang, Tingguo The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.6

Ardipusilloside-I is a natural triterpenoid saponin, which was isolated from Ardisia pusilla A. DC. The aim of the study was to evaluate the stimulation of ardipusilloside-I on gastrointestinal motility in vitro and in vivo. The experiment of smooth muscle contraction directly monitored the contractions of the isolated jejunal segment (IJS) in different contractile states, and the effects of ardipusilloside-I on myosin were measured in the presence of $Ca^{2+}$-calmodulin using the activities of 20 kDa myosin light chain ($MLC_{20}$) phosphorylation and myosin $Mg^{2+}$-ATPase. The effects of ardipusilloside-I on gastro emptying and intestinal transit in constipation-predominant rats were observed, and the MLCK expression in jejuna of constipated rats was determined by western blot. The results showed that, ardipusilloside-I increased the contractility of IJS in a dose-dependent manner and reversed the low contractile state (LCS) of IJS induced by low $Ca^{2+}$, adrenaline, and atropine respectively. There were synergistic effects on contractivity of IJS between ardipusilloside-I and ACh, high $Ca^{2+}$, and histamine, respectively. Ardipusilloside-I could stimulate the phosphorylation of $MLC_{20}$ and $Mg^{2+}$-ATPase activities of $Ca^{2+}$- dependent phosphorylated myosin. Ardipusilloside-I also stimulated the gastric emptying and intestinal transit in normal and constipated rats in vivo, respectively, and increased the MLCK expression in the jejuna of constipation-predominant rats. Briefly, the findings demonstrated that ardipusilloside-I could effectively excite gastrointestinal motility in vitro and in vivo.