Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting

Zhang, Caixia,Wang, Zhenyu,Li, Zheng,Shen, Qingwu,Chen, Lijuan,Gao, Lingling,Zhang, Dequan 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A phosphoproteomic profile of myofibrillar and sarcoplasmic proteins of muscle in response to salting was investigated. Myofibrillar and sarcoplasmic proteins extracted from salted meat with 0, 1, 2, 3, 4, and 5% salt for 0, 2, 4, 6, 8, and 16 h were analyzed by SDS-PAGE electrophoresis and fluorescence staining. The global phosphorylation of myofibrillar proteins in salted meat was lower than that in control muscle at 16 h of salting (p<0.05), and the global phosphorylation of myofibrillar proteins in 3% salt-treated group at 16 h was the lowest. However, salting showed no significant effect on phosphorylation of sarcoplasmic proteins. Four categories of phosphorylated protein were identified by LC-MS/MS, involved in stress response (heat shock protein), glycometabolism (glycogen phosphorylase, glyceraldehyde-3-phosphate dehydrogenase), oxidation or reduction (superoxide dismutase), and others (myoglobin), the phosphorylation of which was affected by salting. Thus, salting may influence meat quality through protein phosphorylation, which regulates protein degradation and glycolysis.

Multi-objective Fuzzy-optimization of Crowbar Resistances for the Low-Voltage Ride-through of Doubly Fed Induction Wind Turbine Generation Systems

Zhang, Wenjuan,Ma, Haomiao,Zhang, Junli,Chen, Lingling,Qu, Yang The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.4

This study investigates the multi-objective fuzzy optimization of crowbar resistance for the doubly fed induction generator (DFIG) low-voltage ride-through (LVRT). By integrating the crowbar resistance of the crowbar circuit as a decision variable, a multi-objective model for crowbar resistance value optimization has been established to minimize rotor overcurrent and to simultaneously reduce the DFIG reactive power absorbed from the grid during the process of LVRT. A multi-objective genetic algorithm (MOGA) is applied to solve this optimization problem. In the proposed GA, the value of the crowbar resistance is represented by floating-point numbers in the GA population. The MOGA emphasizes the non-dominated solutions and simultaneously maintains diversity in the non-dominated solutions. A fuzzy-set-theory-based is employed to obtain the best solution. The proposed approach has been evaluated on a 3 MW DFIG LVRT. Simulation results show the effectiveness of the proposed approach for solving the crowbar resistance multi-objective optimization problem in the DFIG LVRT.

Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting

Caixia Zhang,Zhenyu Wang,Zheng Li,Qingwu Shen,Lijuan Chen,Lingling Gao,Dequan Zhang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A phosphoproteomic profile of myofibrillar and sarcoplasmic proteins of muscle inresponse to salting was investigated. Myofibrillar and sarcoplasmic proteins extracted from saltedmeat with 0, 1, 2, 3, 4, and 5% salt for 0, 2, 4, 6, 8, and 16 h were analyzed by SDS-PAGE electrophoresisand fluorescence staining. The global phosphorylation of myofibrillar proteins in salted meat was lowerthan that in control muscle at 16 h of salting (p<0.05), and the global phosphorylation of myofibrillarproteins in 3% salt-treated group at 16 h was the lowest. However, salting showed no significant effecton phosphorylation of sarcoplasmic proteins. Four categories of phosphorylated protein wereidentified by LC-MS/MS, involved in stress response (heat shock protein), glycometabolism (glycogenphosphorylase, glyceraldehyde-3-phosphate dehydrogenase), oxidation or reduction (superoxidedismutase), and others (myoglobin), the phosphorylation of which was affected by salting. Thus,salting may influence meat quality through protein phosphorylation, which regulates proteindegradation and glycolysis.

Multi-objective Fuzzy-optimization of Crowbar Resistances for the Low-Voltage Ride-through of Doubly Fed Induction Wind Turbine Generation Systems

Wenjuan Zhang,Haomiao Ma,Junli Zhang,Lingling Chen,Yang Qu 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.4

This study investigates the multi-objective fuzzy optimization of crowbar resistance for the doubly fed induction generator (DFIG) low-voltage ride-through (LVRT). By integrating the crowbar resistance of the crowbar circuit as a decision variable, a multi-objective model for crowbar resistance value optimization has been established to minimize rotor overcurrent and to simultaneously reduce the DFIG reactive power absorbed from the grid during the process of LVRT. A multi-objective genetic algorithm (MOGA) is applied to solve this optimization problem. In the proposed GA, the value of the crowbar resistance is represented by floating-point numbers in the GA population. The MOGA emphasizes the non-dominated solutions and simultaneously maintains diversity in the non-dominated solutions. A fuzzy-set-theory-based is employed to obtain the best solution. The proposed approach has been evaluated on a 3 MW DFIG LVRT. Simulation results show the effectiveness of the proposed approach for solving the crowbar resistance multi-objective optimization problem in the DFIG LVRT.

Preparation of Intumescent Flame Retardant Poly(butylene succinate) Using Urea Intercalated Kaolinite as Synergistic Agent

Lingling Gu,Sheng Zhang,Hongfei Li,Jun Sun,WuFei Tang,Liqian Zhao,Xiaoyu Gu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.8

Urea (U) was successfully intercalated into Kaolinite (Kaol) and the product K-U was introduced intopoly(butylene succinate) (PBS)/intumescent flame retardants (IFR) by melt blending. The structure of K-U was characterizedby X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The flame retardancy, thermal degradation,and combustion behavior of PBS composites were characterized by limiting oxygen index (LOI), vertical burning test to getUL-94 rating, thermogravimetricanalysis (TGA), and cone calorimeter test (Cone). With the introduction of K-U and IFRinto PBS, the LOI obviously increased from 21.9 % for neat PBS to 40.1 % for composite containing 20 wt% IFR/5 wt% KU;PBS cannot pass any UL-94 rating but the composite passed UL-94 V-0 rating. Similarly, the peak heat release rate(PHRR) in cone test decreased from 576 kW/m2 to 292 kW/m2. SEM results showed the char of the composite became morecompact and smooth.

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Lingling Zhou,Yuxi Di,Mingfei Zhang,Yichang Chen,Ruonan Sun,Meiyu Shen,Fengxiang Tian,Pei Yang,Feiya Qian 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.1

Purpose: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, and Th17 cells are key factors in the pathogenesis of human inflammatory conditions, such as RA. Catalpol (CAT), a component in Rehmanniae Radix (RR), has been found to regulate human immunity. However, the effects of CAT on Th17 cell differentiation and improvement of RA are not clear. Materials and Methods: Collagen-induced arthritis (CIA) mice were constructed to detect the effects of CAT on arthritis and Th17 cells. The effect of CAT on Th17 differentiation was evaluated with let-7g-5p transfection experiments. Flow cytometry was used to detect the proportion of Th17 cells after CAT treatment. Levels of interleukin-17 and RORγt were assessed by qRT-PCR and enzyme-linked immunosorbent assay. The expression of signal transducer and activator of transcription 3 (STAT3) was determined by qRT-PCR and Western blot. Results: We found that the proportion of Th17 cells was negatively associated with let-7g-5p expression in CIA mice. In in vitro experiments, CAT suppressed traditional differentiation of Th17 cells. Simultaneously, CAT significantly decreased Tregs-to-Th17 cells transdifferentiation. Our results demonstrated that CAT inhibited Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p and that the suppressive effect of CAT on traditional differentiation of Th17 cells is not related with let-7-5p. Conclusion: Our data indicate that CAT may be a potential modulator of Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p to reduce the expression of STAT3. These results provide new directions for research into RA treatment.

Eco-friendly Grafting of Chitosan as a Biopolymer onto Wool Fabrics Using Horseradish Peroxidase

Lingling Xu,Nan Zhang,Qiang Wang,Jiugang Yuan,Yuanyuan Yu,Ping Wang,Xuerong Fan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

Chitosan and enzymes have been extensively used in modification of wool, due to their non-toxic and eco-friendly characteristics, as well as to reduce pollution in textile production. In the present work, chitosan was grafted onto wool fabrics using horseradish peroxidase (HRP) in order to endow wool with outstanding properties. The reaction mechanism, grafting percentage, and properties of chitosan-grafted pretreated wool fabric including wettability, dyeability, shrink resistance, and antibacterial activity were studied. P-hydroxyphenylacetamide (PHAD), as a model compound for tyrosine residues in wool,was used to study the mechanism of HRP-mediated grafting of chitosan onto wool. UV and FTIR analyses indicated that quinones, which are intermediate of HRP/H2O2-catalyzed PHAD, reacted with the amino groups of chitosan by Schiff base or Michael addition reactions. Scanning electron microscopy (SEM) showed that dichloroisocyanuric acid pretreatment had an etching effect and some substances were deposited onto the wool fiber. SEM and Fourier transform infrared (FTIR) spectroscopy further confirmed the covalent grafting of chitosan onto wool. Compared with chitosan-grafted unpretreatedwool, there was an obvious improvement in grafting efficiency of chitosan onto wool fabric, physical and mechanical properties after pretreatment and chitosan grafting on the wool. For chitosan-grafted pretreated wool fabrics, there was no observable change in the thermal behavior, while the hydrophilicity, dyeability, shrinkage resistance, and antibacterial activity were remarkably improved compared to that of untreated wool. The present work offers a new ecologically acceptable process of grafting chitosan on the wool.

Synergistic interaction of b-galactosyl-pyrrolidinyl diazeniumdiolate with cisplatin against three tumor cells

Lingling Deng,Erli Zhang,Chang Chen 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.5

Cisplatin is a platinum-based compound that islargely employed as an effective antitumor drug against awide spectrum of solid neoplasms for many years. Despiteof its initial therapeutic success, cisplatin often results inhigh incidence of chemoresistance and high-dose cytotoxicity. Consequently, considerable efforts in hopes ofreducing the dose-dependent side effects of cisplatin whileretaining, or even enhancing, its antitumor properties havebeen undertaken throughout the past three decades. Nitricoxide (NO) is a small lipophilic free radical gas possessingversatile biological functions, including antitumor activities. However, NO, of itself, is difficult to be used, becauseof its extreme instability and short half-life. Previously, wehave reported a stable NO donor, b-galactosyl-pyrrolidinyldiazeniumdiolate (b-Gal-NONOate), which exerts tumorkilling effects through site-specific intracellular release ofexogenous NO. In this study, we further investigated thecombined inhibitory effect of b-Gal-NONOate and cisplatinagainst C6/LacZ, 9L/LacZ, and HeLa/LacZ tumorcells. It was shown that, in combination with b-Gal-NONOate, the antitumor effects of cisplatin against thesecommon tumor cell lines were increased in a dose-dependentmanner. Furthermore, the combination of thesechemicals resulted in a synergistic suppression on tumorgrowth, which was achieved under a much lower cisplatindosage. Collectively, our findings indicate that b-Gal-NONOate can synergistically improve the antitumor effectof cisplatin, and may therefore reduce its side effectscaused by high dose cisplatin monochemotherapies. Accordingly, b-Gal-NONOate is an important therapeuticassistant reagent with great potential of clinical applicability,and thus worth of continuous research in the comingfuture.

A Modeling and Analysis Method for CCM Fractional Order Buck‑Boost Converter by Using R–L Fractional Defnition

Lingling Xie,Zhipei Liu,Bo Zhang 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

Fractional defnition is important for the application of fractional-order theory in electrical engineering, and fractional-order theory directly afects the accuracy of the model and the operating characteristics of the power converter. Most researchers used the fractional defnition of Caputo to study fractional order power converter. However, it is found that the model based on Caputo fractional defnition is inconsistent with the actual situation. A novel modeling and analysis method for the fractional buck–boost converter is proposed in this paper. Firstly, the fractional converter model operating in continuous conduction mode (CCM) is established and the DC analysis is completed by using Riemann–Liouville (R–L) fractional defnition. The R–L fractional defnition is nearly 1.1% more accurate than the Caputo defnition in the DC analysis. Secondly, the infuence of the capacitor and inductor orders on the converter is performed. The results show that the capacitor and inductor orders have great efect on the DC components of the state variable and the steady-state characteristics of the fractional order buckboost converter. Finally, simulation and experiment results are provided that the validity of theoretical analysis.

Synthesis and Properties of Arylacetylene Resins with Siloxane Units

Fei Gao,Lingling Zhang,Lemin Tang,Jian Zhang,Yan Zhou,Farong Huang,Lei Du 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.4

A series of arylacetylene resins with siloxane units were synthesized by the condensation reactions of m-diethynylbenzene magnesium reagents with various α,ω-bis(chloro)dimethylsiloxanes. These resins are liquids and are miscible with common organic solvents at room temperature. The structures of the resins were characterized by FT-IR, 1H NMR,13C NMR, 29Si NMR, and gel permeation chromatography (GPC). The thermal behaviors of the resins were examined with differential scanning calorimetry (DSC). These resins have good processability. They can be thermally crosslinked through the ethynyl groups to produce cured resins. The thermal and thermooxidative stabilities of the cured resins were studied by thermogravimetric analysis (TGA). The cured resins possess high thermal and thermooxidative stability. Their decomposition occurs at above 500 oC in both N2 and air. With increasing the length of siloxane units in the resins, the thermal stability of the cured resins decreases in N2. When the cured resins were sintered above 1450 oC under argon, hard and glassy SiOC ceramics were obtained. These SiOC ceramics have the decomposition temperatures at 5% weight loss above 800 oC in air.