Risk Factors for Cervical Cancer in Rural Areas of Wuhan China: a Matched Case-control Study

Zhang, Bin,Zhou, Ai-Fen,Zhu, Chang-Cai,Zhang, Ling,Xiang, Bing,Chen, Zhong,Hu, Rong-Hua,Zhang, Ya-Qi,Qiu, Lin,Zhang, Yi-Ming,Xiong, Chao-Du,Du, Yu-Kai,Shi, Yu-Qin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Cervical cancer is a serious public health problem in developing countries. We investigated possible risk factors for cervical cancer in rural areas of Wuhan China using a matched case-control study with 33 women diagnosed with cervical cancer and 132 healthy women selected from the same area as matched controls. A questionnaire, which included questions about general demography conditions, environmental and genetic factors, the first sexual intercourse, first marriage age, age at first pregnancy, pregnancy first child's age, female personal health history, social psychological factors, dietary habits, smoking and alcohol status and other living habits was presented to all participants. At the same time, HPV infection of every participant was examined in laboratory testing. Results showed HPV infection (P<0.000, OR=23.4) and pregnancy first child's age (P<0.000, OR=13.1) to be risk factors for cervical cancer. Menopause (P=0.003, OR=0.073) was a protective factor against cervical cancer. However, there was no indication of associations of environmental (drinking water, insecticide, disinfectant) genetic (cancer family history), or life-style factors (smoking status, alcohol status, physical training, sleep quality), including dietary habits (intake of fruit and vegetable, meat, fried food, bean products and pickled food) or social psychological factors with cervical cancer. The results suggest that the risk of cervical cancer in Chinese rural women may be associated with HPV infection, menopause and the pregnancy first child's age.

Inhibition of Transient Receptor Potential Melastain 7 Enhances Apoptosis Induced by TRAIL in PC-3 cells

Lin, Chang-Ming,Ma, Ji-Min,Zhang, Li,Hao, Zong-Yao,Zhou, Jun,Zhou, Zhen-Yu,Shi, Hao-Qiang,Zhang, Yi-Fei,Shao, En-Ming,Liang, Chao-Zhao Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Transient receptor potential melastain 7 (TRPM7) is a bifunctional protein with dual structure of both ion channel and protein kinase, participating in a wide variety of diseases including cancer. Recent researches have reported the mechanism of TRPM7 in human cancers. However, the correlation between TRPM7 and prostate cancer (PCa) has not been well studied. The objective of this study was to investigate the potential the role of TRPM7 in the apoptosis of PC-3 cells, which is the key cell of advanced metastatic PCa. In this study, we demonstrated the influence and potential function of TRPM7 on the PC-3 cells apoptosis induced by TNF-related apoptosis inducing-ligand (TRAIL). The study also found a novel up-regulated expression of TRPM7 in PC-3 cells after treating with TRAIL. Suppression of TRPM7 by TRPM7 non-specific inhibitors ($Gd^{3+}$ or 2-aminoethoxy diphenylborate (2-APB) ) not only markedly eliminated TRPM7 expression level, but also increased the apoptosis of TRAIL-treated PC-3 cells, which may be regulated by the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway accompany with up-regulated expression of cleaved Caspase-3, (TRAIL-receptor 1, death receptors 4) DR4, and (TRAIL-receptor 2, death receptors 5) DR5. Taken together, our findings strongly suggested that TRPM7 was involved in the apoptosis of PC-3 cells induced by TRAIL, indicating that TRPM7 may be applied as a therapeutic target for PCa.

CNOP-Based Sensitive Areas Identification for Tropical Cyclone Adaptive Observations with PCAGA Method

Lin-Lin Zhang,Shi-Jin Yuan,Bin Mu,Fei-Fan Zhou 한국기상학회 2017 Asia-Pacific Journal of Atmospheric Sciences Vol.53 No.1

In this paper, conditional nonlinear optimal perturbation (CNOP) was investigated to identify sensitive areas for tropical cyclone adaptive observations with principal component analysis based genetic algorithm (PCAGA) method and two tropical cyclones, Fitow (2013) and Matmo (2014), were studied with a 120 km resolution using the fifth-generation Mesoscale Model (MM5). To verify the effectiveness of PCAGA method, CNOPs were also calculated by an adjoint-based method as a benchmark for comparison on patterns, energies, and vertical distributions of temperatures. Comparing with the benchmark, the CNOPs obtained from PCAGA had similar patterns for Fitow and a little different for Matmo; the vertically integrated energies were located closer to the verification areas and the initial tropical cyclones. Experimental results also presented that the CNOPs of PCAGA had a more positive impact on the forecast improvement, which gained from the reductions of the CNOPs in the whole domain containing sensitive areas. Furthermore, the PCAGA program was executed 40 times for each case and all the averages of benefits were larger than the benchmark. This also proved the validity and stability of the PCAGA method. All results showed that the PCAGA method could approximately solve CNOP of complicated models without computing adjoint models, and obtain more benefits of reducing the CNOPs in the whole domain.

Overexpression of NDRG2 Can Inhibit Neuroblastoma Cell Proliferation through Negative Regulation by CYR61

Zhang, Zhi-Guo,Li, Gang,Feng, Da-Yun,Zhang, Jian,Zhang, Jing,Qin, Huai-Zhou,Ma, Lian-Ting,Gao, Guo-Dong,Wu, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Several recent studies have showed that the n-myc downstream regulated gene 2 (NDRG2) is a new tumor suppressor gene, and that it plays an important role in tumor suppression in several cancers or cancer cell lines. However, few studies focused on its function in neuroblastoma cells. In the present investigation, we demonstrated that NDRG2 overexpression inhibited their proliferation. Using a cDNA microarray, we found that overexpression of NDRG2 inhibited the expression of cysteine-rich protein 61 (CYR61), a proliferation related gene. From our research, CYR61 may partially hinder NDRG2-mediated inhibition of cell proliferation. Overexpression of NDRG2 resulted in accumulation of cells in the G1 phase, which was accompanied by upregulation of p21 and p27 and downregulation of CDK4 and cyclin D1. Taken together, these data indicate that NDRG2 inhibits the proliferation of neuroblastoma cells partially through suppression of CYR61. Our findings offer novel insights into the physiological roles of NDRG2 in neuroblastoma cell proliferation, and NDRG2 may prove to be effective candidate for the treatment of children with neuroblastoma.

Kinetics and Modeling for Extraction of Chrysin from Oroxylum indicum Seeds

Li Zhou,Ting Jing,Peipei Zhang,Liqin Zhang,Shaona Cai,Tingting Liu,Huihui Fan,Guangde Yang,Rong Lin,Jiye Zhang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Research on extraction of chrysin is crucial for theoretical purposes and for food industrial bioprocesses. Optimization and kinetics of chrysin extraction from seeds of Oroxylum indicum (L.) Vent. were analyzed using agitated solid-liquid extractions with ethanol and water mixtures. The influence of extraction process parameters was investigated. Optimized conditions for chrysin extraction were a 0.2 mole fraction of ethanol as an extraction solvent, a temperature of 318 K, an agitation speed 1,400 rpm, and a solid to solvent ratio of 1:30. The extraction kinetic behavior of chrysin followed first order kinetics. The kinetic expression developed by Spiro and Siddique was used and the model was in agreement with experimental results. The diffusion coefficient ranged from 1.38×10−11 to 19.43×10−11 m2·s−1 and the activation energy for extraction kinetics was 21.85 kJ·mol−1.

Top-gate amorphous IGZO thin-film transistors with a SiO buffer layer inserted between active channel layer and gate insulator

F. Zhou,H.P. Lin,L. Zhang,J. Li,X.W. Zhang,D.B. Yu,X.Y. Jiang,Z.L. Zhang 한국물리학회 2012 Current Applied Physics Vol.12 No.1

In this paper, top-gate thin-film transistors (TFTs) using amorphous IneGaeZneO as the n-channel active layer and SiO2 as gate insulator were fabricated by radio frequency magnetron sputtering at room temperature. In this device, a SiO layer was used to be a buffer layer between active layer and gate insulator for preventing the damage of the InGaZnO surface by the process of sputtering SiO2 with relatively high sputtering power. The thickness of buffer layers was studied and optimized for enhancing the TFTs performances. Contrasting to the TFTs without buffer layer, the optimized thickness of 10 nm SiO buffer layer improved the top-gate TFTs performances greatly: mobility increases 30%, reached 1.29 cm2/V s, the Ion/Ioff ratio increases 3 orders, and the trap density at the interface of channel/insulator decreases about 1 order, indicated that the improvement of semiconductor/dielectric interface by buffering the sputtering power.

PVA Hydrogel Functionalization via PET-RAFT Grafting with Glycidyl Methacrylate and Immobilization with 2-Hydroxypropyltrimethyl Ammonium Chloride Chitosan via Ring-Open Reaction

Jinsheng Zhou,Yanming Lin,Lin Ye,Ling Wang,Li Zhou,Huiyuan Hu,Qilong Zhang,Hui Yang,Zhongkuan Luo 한국고분자학회 2019 Macromolecular Research Vol.27 No.11

To solve the biofouling problem of polyvinyl alcohol (PVA) hydrogel as the artificial cornea, glycidyl methacrylate (GMA) and 2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) were grafted on the surface of PVA hydrogel via a new method of photoinduced electron transfer–reversible addition fragmentation chain transfer (PET-RAFT) polymerization and ring-open reaction. Both attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM), and thermogravimetric analysis (TGA) confirmed that GMA and HACC were successfully grafted on the surface of PVA hydrogel. A series of experiments to test the hydrophilicity of PVA hydrogel showed that it became hydrophobic due to the introduction of hydrophobic groups after grafting with GMA and HACC. In addition, cytotoxicity in vitro of PVA-g-p(GMA-HACC) hydrogel could be considered as not cytotoxicity according to ISO 10993-5: 2009. The anti-fouling property of hydrogel decreased after grafting with GMA due to the hydrophobic surface, while increased after grafting with HACC due to the steric repulsion of p(GMA-HACC) polymer brush. It’s no doubt that PET-RAFT was a feasible and reliable surface modification method which could be used in many biomolecules due to the excellent advantages.

Effects of alanyl-glutamine supplementation on the small intestinal mucosa barrier in weaned piglets

Xing, Shen,Zhang, Bolin,Lin, Meng,Zhou, Ping,Li, Jiaolong,Zhang, Lin,Gao, Feng,Zhou, Guanghong Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: The study was to investigate the effects of alanyl-glutamine (Ala-Gln) and glutamine (Gln) supplementation on the intestinal mucosa barrier in piglets. Methods: A total of 180 barrows with initial weight $10.01{\pm}0.03kg$ were randomly allocated to three treatments, and each treatment consisted of three pens and twenty pigs per pen. The piglets of three groups were fed with control diet [0.62% alanine (Ala)], Ala-Gln diet (0.5% Ala-Gln), Gln diet (0.34% Gln and 0.21% Ala), respectively. Results: The results showed that in comparison with control diet, dietary Ala-Gln supplementation increased the height of villi in duodenum and jejunum (p<0.05), Gln supplementation increased the villi height of jejunum (p<0.05), Ala-Gln supplementation up-regulated the mRNA expressions of epidermal growth factor receptor and insulin-like growth factor 1 receptor in jejunal mucosa (p<0.05), raised the mRNA expressions of Claudin-1, Occludin, zonula occludens protein-1 (ZO-1) and the protein levels of Occludin, ZO-1 in jejunal mucosa (p<0.05), Ala-Gln supplementation enlarged the number of goblet cells in duodenal and ileal epithelium (p<0.05), Gln increased the number of goblet cells in duodenal epithelium (p<0.05) and Ala-Gln supplementation improved the concentrations of secretory immunoglobulin A and immunoglobulin G in the jejunal mucosa (p<0.05). Conclusion: These results demonstrated that dietary Ala-Gln supplementation could maintain the integrity of small intestine and promote the functions of intestinal mucosa barriers in piglets.

A high performance of BPhen-based white organic light-emitting devices with a dual-emitting layer and its electroluminescent spectral property

Hua-Ping Lin,Fan Zhou,Jun Li,Xiao-Wen Zhang,Dong-Bin Yu,Liang Zhang,Xue-Yin Jiang,Zhi-Lin Zhang 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.4

This work demonstrated the fabrication of white organic light-emitting devices (WOLEDs) using a dual emitting layer (d-EML) consisting of blue and ‘white’ emitters. In this d-EML system, the blue emitter not only emits but also assists the incomplete energy transfer. More importantly, it behaves as an effective trapping site for holes, which contributes to the efficient recombination of electron–hole pairs. The d-EML was constructed between the hole-transmitting layer (HTL) and the electron-transmitting layer (ETL) of Alq3 and BPhen. The thickness of the blue emitter used in the d-EML devices has an important effect on chromaticity and efficiency. Through the optimization of device structure, the reasonable white emission with Commission Internationale de L’Eclairage (CIE) color coordinates of (0.33, 0.33) and little color shift was obtained. The device showed an applicable luminance with its maximum luminance of 22,874 cd/m2 at a driving voltage of 16 V. The maximum luminance efficiency was achieved 8.10 cd/A,and the maximum power efficiency was reached 5.07 Lm/W. The result is explained with the help of the excitons generation and diffusion theory. According to the theory of excitons generation and diffusion,an equation has been set up which concerns electroluminescent spectra to the thickness of the two emitters and to the exciton diffusion length.

Elucidations of weak microcavity effect and improved pixel contrast ratio in Si-based top-emitting organic light-emitting diode

Xiao-Wen Zhang,Hua-Ping Lin,Jun Li,Fan Zhou,Bin Wei,Xue-Yin Jiang,Zhi-Lin Zhang 한국물리학회 2012 Current Applied Physics Vol.12 No.5

Microcavity effect and pixel contrast ratio (PCR) are key issues for top-emitting organic light-emitting diode (TOLED). Si and Ag are two widely used reflective anodes for constructing effective TOLEDs. The mechanism contributing to microcavity effect and PCR has been clarified by using conventional optoelectronic theory. Consequently, the differences of microcavity effect and PCR between Si-based and Agbased TOLEDs are discovered. Our results indicate that the Si-based TOLED possesses weak microcavity effect and improved PCR in comparison with Ag-based device. This is resulted from the low reflectivity and less reflective phase change of Si. The weak microcavity effect in Si-based TOLED also contributes to negligible variation of electroluminescent spectra with viewing angles and low device efficiency. Furthermore, Si-based TOLED shows rather high PCR which is about quintuple that of Ag-based device. Microcavity effect and pixel contrast ratio (PCR) are key issues for top-emitting organic light-emitting diode (TOLED). Si and Ag are two widely used reflective anodes for constructing effective TOLEDs. The mechanism contributing to microcavity effect and PCR has been clarified by using conventional optoelectronic theory. Consequently, the differences of microcavity effect and PCR between Si-based and Agbased TOLEDs are discovered. Our results indicate that the Si-based TOLED possesses weak microcavity effect and improved PCR in comparison with Ag-based device. This is resulted from the low reflectivity and less reflective phase change of Si. The weak microcavity effect in Si-based TOLED also contributes to negligible variation of electroluminescent spectra with viewing angles and low device efficiency. Furthermore, Si-based TOLED shows rather high PCR which is about quintuple that of Ag-based device.