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Studies on the storage stability of fermented red dragon fruit (Hylocereus polyrhizus) drink
Kah Yee Choo,Caryn Kho,Renee Lay Hong Lim,Chun Wai Ho,Yien Yien Ong,Yin Yin Thoo,Chin Ping Tan 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.5
The objective of this work was to study the effect of storage temperatures and duration on the stability of fermented red dragon fruit drink (FRDFD) on its betacyanins content, physicochemical and microbiological qualities (BPM) and determining sensory acceptability. Results showed that both storage temperatures and duration have a significant effect on betacyanins content and physicochemical properties of FRDFD. Aerobic mesophilic and yeast and mold counts were lower than 1 9 103 CFU/ mL for FRDFD stored at both temperatures. The loss of betanin (16.53–13.93 g/L) at 4 C was 15.73% with no significant changes in physicochemical properties from week two onwards compared to 56.32% (16.53–7.22 g/L) of betanin loss at 25 C. At week eight, FRDFD stored at 4 C still contained 13.93 g/L betanin with a pH value of 3.46, suggested its potential as a functional drink which is sensory acceptable (mean score[80% using hedonic test) among consumers.
Study on bioaccessibility of betacyanins from red dragon fruit (Hylocereus polyrhizus)
Kah Yee Choo,Yien Yien Ong,Renee Lay Hong Lim,Chin Ping Tan,Chun Wai Ho 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4
Betacyanins are bioactive dietary phytochemicalswhich can be found in red dragon fruit (RDF). Therefore, the bioaccessibility of betacyanins that presentin fermented red dragon fruit drink (RDFD) and pressedred dragon fruit juice (RDFJ) was accessed in simulatedgastric and intestinal digestion. Results disclosed thatbetacyanins from RDFD and RDFJ suffered minor loss(\25%) at gastric-like environment but greater loss wasobserved during the intestinal phase digestion. After subjectedto intestinal digestion, RDFD retained 46.42% ofbetanin while RDFJ retained 43.76%, with betanin concentrationof 17.12 mM and 12.37 mM, respectively. Findings also revealed that RDFD exhibited higherantioxidant capacity compared to RDFJ after subjected tointestinal digestion, with values of 0.88 mM Troloxequivalent antioxidant capacity (TEAC) and 0.85 mMTEAC, respectively. The data suggests that betacyaninsthat present in RDF are bioaccessible while fermentationable to enhance the bioavailability with more betacyaninsretained after intestinal digestion.
Chuah Tyng Yu,Lim Chun Yee,Tan Rui Zhen,Pratumvinit Busadee,Loh Tze Ping,Vasikaran Samuel,Markus Corey 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.5
Functional reference limits describe key changes in the physiological relationship between a pair of physiologically related components. Statistically, this can be represented by a significant change in the curvature of a mathematical function or curve (e.g., an observed plateau). The point at which the statistical relationship changes significantly is the point of curvature inflection and can be mathematically modeled from the relationship between the interrelated biomarkers. Conceptually, they reside between reference intervals, which describe the statistical boundaries of a single biomarker within the reference population, and clinical decision limits that are often linked to the risk of morbidity or mortality and set as thresholds. Functional reference limits provide important physiological and pathophysiological insights that can aid laboratory result interpretation. Laboratory professionals are in a unique position to harness data from laboratory information systems to derive clinically relevant values. Increasing research on and reporting of functional reference limits in the literature will enhance their contribution to laboratory medicine and widen the evidence base used in clinical decision limits, which are currently almost exclusively contributed to by clinical trials. Their inclusion in laboratory reports will enhance the intellectual value of laboratory professionals in clinical care beyond the statistical boundaries of a healthy reference population and pave the way to them being considered in shaping clinical decision limits. This review provides an overview of the concepts related to functional reference limits, clinical examples of their use, and the impetus to include them in laboratory reports.
Jaeyoung Cho,Pil-Jae Kong,Wanjoo Chun,Yeo-Ok Moon,Yee-Tae Park,So-Young Lim,Sung-Soo Kim 대한생리학회-대한약리학회 2003 The Korean Journal of Physiology & Pharmacology Vol.13 No.4
Kainic acid (KA) is a structural analogue of glutamate that interacts with specific presynaptic and postsynaptic receptors to potentiate the release and excitatory actions of glutamate. Systemic or intracerebroventricular (i.c.v.) administration of KA to experimental animals elicits multifocal seizures with a predominantly limbic localization, and results in neuronal death of cornu ammonia 1 (CA1), reactive gliosis and biochemical changes in the hippocampus and other limbic structures. Several lines of evidence suggest that reactive oxygen species (ROS) play a pivotal role in the pathogenesis of excitotoxic death by KA. Curcumin has been known to possess anti-oxidative and anti-inflammatory activities. In this study, the effects of curcumin on KA induced hippocampal cell death, reactive gliosis and biochemical changes in reactive glia were investigated by immunohistochemical methods. Our data demonstrated that curcumin attenuated KA-induced astroglial and microglial activation although it did not protect KA-induced hippocampal cell death.
An Objective Approach to Deriving the Clinical Performance of Autoverification Limits
Loh Tze Ping,Tan Rui Zhen,Lim Chun Yee,Markus Corey 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.5
This study describes an objective approach to deriving the clinical performance of autoverification rules to inform laboratory practice when implementing them. Anonymized historical laboratory data for 12 biochemistry measurands were collected and Box-Cox-transformed to approximate a Gaussian distribution. The historical laboratory data were assumed to be error-free. Using the probability theory, the clinical specificity of a set of autoverification limits can be derived by calculating the percentile values of the overall distribution of a measurand. The 5th and 95th percentile values of the laboratory data were calculated to achieve a 90% clinical specificity. Next, a predefined tolerable total error adopted from the Royal College of Pathologists of Australasia Quality Assurance Program was applied to the extracted data before subjecting to Box-Cox transformation. Using a standard normal distribution, the clinical sensitivity can be derived from the probability of the Z-value to the right of the autoverification limit for a one-tailed probability and multiplied by two for a two-tailed probability. The clinical sensitivity showed an inverse relationship with between-subject biological variation. The laboratory can set and assess the clinical performance of its autoverification rules that conforms to its desired risk profile.
Cho, Jae-Young,Kong, Pil-Jae,Chun, Wan-Joo,Moon, Yeo-Ok,Park, Yee-Tae,Lim, So-Young,Kim, Sung-Soo The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.6
Kainic acid (KA) is a structural analogue of glutamate that interacts with specific presynaptic and postsynaptic receptors to potentiate the release and excitatory actions of glutamate. Systemic or intracerebroventricular (i.c.v.) administration of KA to experimental animals elicits multifocal seizures with a predominantly limbic localization, and results in neuronal death of cornu ammonia 1 (CA1), reactive gliosis and biochemical changes in the hippocampus and other limbic structures. Several lines of evidence suggest that reactive oxygen species (ROS) play a pivotal role in the pathogenesis of excitotoxic death by KA. Curcumin has been known to possess anti-oxidative and anti-inflammatory activities. In this study, the effects of curcumin on KA induced hippocampal cell death, reactive gliosis and biochemical changes in reactive glia were investigated by immunohistochemical methods. Our data demonstrated that curcumin attenuated KA-induced astroglial and microglial activation although it did not protect KA-induced hippocampal cell death.
Calibration Practices in Clinical Mass Spectrometry: Review and Recommendations
Cheng Wan Ling,Markus Corey,Lim Chun Yee,Tan Rui Zhen,Sethi Sunil Kumar,Loh Tze Ping 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.1
Background: Calibration is a critical component for the reliability, accuracy, and precision of mass spectrometry measurements. Optimal practice in the construction, evaluation, and implementation of a new calibration curve is often underappreciated. This systematic review examined how calibration practices are applied to liquid chromatography-tandem mass spectrometry measurement procedures. Methods: The electronic database PubMed was searched from the date of database inception to April 1, 2022. The search terms used were “calibration,” “mass spectrometry,” and “regression.” Twenty-one articles were identified and included in this review, following evaluation of the titles, abstracts, full text, and reference lists of the search results. Results: The use of matrix-matched calibrators and stable isotope-labeled internal standards helps to mitigate the impact of matrix effects. A higher number of calibration standards or replicate measurements improves the mapping of the detector response and hence the accuracy and precision of the regression model. Constructing a calibration curve with each analytical batch recharacterizes the instrument detector but does not reduce the actual variability. The analytical response and measurand concentrations should be considered when constructing a calibration curve, along with subsequent use of quality controls to confirm assay performance. It is important to assess the linearity of the calibration curve by using actual experimental data and appropriate statistics. The heteroscedasticity of the calibration data should be investigated, and appropriate weighting should be applied during regression modeling. Conclusions: This review provides an outline and guidance for optimal calibration practices in clinical mass spectrometry laboratories.
Bethesda system에 의한 "atypical squamous cells of undertermined significance"의 평가
김의정,홍성란,김희숙,박종숙,김계현,임경호,심재욱,박종택,전종수,Kim, Yee-Jeong,Hong, Sung-Ran,Kim, Hy-Sook,Park, Jong-Sook,Kim, Kye-Hyun,Lim, Kyung-Ho,Shim, Jae-Uk,Park, Chong-Taik,Chun, Chong-Soo 대한세포병리학회 1993 대한세포병리학회지 Vol.4 No.2
The recently proposed Bethesda system for cervical/vaginal cytology has made a standardization related to "atypia". In cellular changes due to inflammation or repair, the word "benign cellular change" has been suggested as a substitute for atypia. Terminology related to atypical cells may become standardized, but the cytologic criteria has not been well defined yet. We evaluated 160 cases of atypical squamous cells of undetermined significance(ASCUS) by the Bethesda Sys4em(TBS). Among 30,428 cases screened, a cytologic diagnosis of ASCUS was made in 498 cases(1.6%) and 160 cases were histologically verified ASCUS was diagnosed based on nuclear enlargement and nonclassical signs of condyloma. The results are as follows: One hundred and twenty three cases(76.9%) revealed chronic cervicitis. Thirty seven cases(23.1%) demonstrated squamous intraepithelial lesion. Among intraepithelial lesions, condyloma and mild dysplasia were 28 cases(75.7%). Moderate and severe dysplasia were 5 cases(13.5%) and 4 cases(10.8%), respectively. it is concluded that patients with ASCUS should be colposcopically examined.
천세명,김태성,이기택,고준석,임영진,김국기,임언,이봉암,Chun, Sae Myoung,Kim, Tae Sung,Yee, Gi Taek,Koh, Jun Seok,Lim, Young Jin,Kim, Gook Ki,Leem, Won,Rhee, Bong Arm 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.9
Meningioma associated with intratumoral hemorrhage is rarely reported. We present two patients with intratumoral hemorrhage. One 70-year-old man was admitted to our department with a decreased level of consciousness and left hemiparesis. CT scan and MRI scan revealed huge tumor with intratumoral hemorrhage in the frontal lobe. After surgical removal of the tumor, histopathological diagnosis was a meningotheliomatous meningioma. The other seemed patients was 56-year-old woman with headache and vomiting. She showed no specific neurological deficit. CT and MRI scan revealed large size tumor with intratumoral hemorrhage that looks like meningioma. The patient died suddenly before surgery. We reviewed the relevant literature and discussed the possible mechanism of hemorrhage in the meningioma.