Selection of reference genes for RT-qPCR analysis in Trichogramma chilonis (Hymenoptera: Trichogrammatidae)

Xie Lian-Cheng,Tian Jun-Ce,Lu Yan-Hui,Xu Hongxing,Zang Lian-Sheng,Lu Zhongxian,Jin Lin-Hong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Trichogramma chilonis is an important natural enemy for control of various Lepidoperan crop pests. The biology of T. chilonis is well-studied, but the molecular mechanisms of this biology require further study. Screening suitable reference genes is a vital step for use of RT-qPCR to understand underlying molecular physiology. In the present study, nine candidate reference genes including elongation factor 2 (EF2), ribosomal proteins (RPS23, RPL13, and RPL44), malate dehydrogenase (MDH), eukaryotic translation initiation factor 3 subunit F (EIF3F), zinc finger protein 268 (ZFP268), muscle specific protein 20 (MP20), and ATP synthase subunit alpha (ATP5F1A) were evaluated at different conditions including development stage, diet, temperature, and insecticide treat ments. Four common algorithms (the Delta Ct method, geNorm, BestKeeper, and NormFinder) and RefFinder were used to analyze gene expression stability. Our results indicated that two reference genes used for normalization were sufficient, and the optimal combinations were: RPS23 and EF2 for developmental stages, ZFP268 and EF2 for feeding with different diets, ZFP268 and RPL13 for temperature treatments, and EF2 and RPL44 for insec ticide treatments. The results provide preliminary determination of suitable reference gene for standard RT-qPCR analyses in T. chilonis, which might establish the foundation for further molecular biology research.

An iterative approach for time-domain flutter analysis of bridges based on restart technique

Wen-ming Zhang,Kai-rui Qian,Lian Xie,Lian Xie,Yao-jun Ge 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.28 No.3

This paper presents a restart iterative approach for time-domain flutter analysis of long-span bridges using the commercial FE package ANSYS. This approach utilizes the recursive formats of impulse-response-function expressions for bridge's aeroelastic forces. Nonlinear dynamic equilibrium equations are iteratively solved by using the restart technique in ANSYS, which enable the equilibrium state of system to get back to last moment absolutely during iterations. The condition for the onset of flutter instability becomes that, at a certain wind velocity, the amplitude of vibration is invariant with time. A long-span suspension bridge was taken as a numerical example to verify the applicability and accuracy of the proposed method by comparing calculated results with wind tunnel tests. The proposed method enables the bridge designers and engineering practitioners to carry out time-domain flutter analysis of bridges in commercial FE package ANSYS.

Decision-Making of Determining the Start Time of Charging / Discharging of Electrical Vehicle Based on Prospect Theory

Lian Liu,Xiang Lyu,Chuanwen Jiang,Da Xie 대한전기학회 2014 Journal of Electrical Engineering & Technology Vol.9 No.3

The moment when Electrical Vehicle (EV) starts charging or discharging is one of the most important parameters in estimating the impact of EV load on the grid. In this paper, a decisionmaking problem of determining the start time of charging and discharging during allowed period is proposed and studied under the uncertainty of real-time price. Prospect theory is utilized in the decision-making problem of this paper for it describes a kind of decision making behaviors under uncertainty. The case study uses the parameters of Springo SGM7001EV and adopts the historical realtime locational marginal pricing (LMP) data of PJM market for scenario reduction. Prospect values are calculated for every possible start time in the allowed charging or discharging period. By comparing the calculated prospect values, the optimal decisions are obtained accordingly and the results are compared with those based on Expected Utility Theory. Results show that with different initial Stateof-Charge ( 0 SoC ) and different reference points, the optimal start time of charging can be the one between 12 a.m. to 3 a.m. and optimal discharging starts at 2 p.m. or 3p.m. Moreover, the decision results of Prospect Theory may differ from that of the Expected Utility Theory with the reference points changing.

Decision-Making of Determining the Start Time of Charging / Discharging of Electrical Vehicle Based on Prospect Theory

Liu, Lian,Lyu, Xiang,Jiang, Chuanwen,Xie, Da The Korean Institute of Electrical Engineers 2014 Journal of Electrical Engineering & Technology Vol.9 No.3

The moment when Electrical Vehicle (EV) starts charging or discharging is one of the most important parameters in estimating the impact of EV load on the grid. In this paper, a decision-making problem of determining the start time of charging and discharging during allowed period is proposed and studied under the uncertainty of real-time price. Prospect theory is utilized in the decision-making problem of this paper for it describes a kind of decision making behaviors under uncertainty. The case study uses the parameters of Springo SGM7001EV and adopts the historical realtime locational marginal pricing (LMP) data of PJM market for scenario reduction. Prospect values are calculated for every possible start time in the allowed charging or discharging period. By comparing the calculated prospect values, the optimal decisions are obtained accordingly and the results are compared with those based on Expected Utility Theory. Results show that with different initial State-of-Charge ($SoC_0$) and different reference points, the optimal start time of charging can be the one between 12 a.m. to 3 a.m. and optimal discharging starts at 2 p.m. or 3p.m. Moreover, the decision results of Prospect Theory may differ from that of the Expected Utility Theory with the reference points changing.

Effect of crystal structure on adsorption behaviors of nanosized TiO2 for heavy-metal cations

Xiaofeng Xie,Lian Gao 한국물리학회 2009 Current Applied Physics Vol.9 No.3

Adsorption behaviors of Pb(II) and Cd(II) ions on the nanosized titanium dioxide with different crystal structure, such as anatase, rutile, mix crystal (Degussa P25) and amorphism have been explored. The adsorption and desorption experiments of the Pb(II) and Cd(II) ions are carried out in titanium dioxide suspension under different pH value conditions. Adsorption kinetics has been studied with pH dependence isotherms, which indicates that both chemisorption and physisorption are existed in adsorption courses. The results show that the anatase TiO2 powders possess the highest chemisorption capacities among the samples, which can be attributed to its higher surface energy, rough surface and unsaturated oxygen bond. The mechanism of adsorption is suggested to be the soft acid/soft base interactions to form complexes for chemisorption and electrostatic force of electric double layer (EDL) model for physisorption. Adsorption behaviors of Pb(II) and Cd(II) ions on the nanosized titanium dioxide with different crystal structure, such as anatase, rutile, mix crystal (Degussa P25) and amorphism have been explored. The adsorption and desorption experiments of the Pb(II) and Cd(II) ions are carried out in titanium dioxide suspension under different pH value conditions. Adsorption kinetics has been studied with pH dependence isotherms, which indicates that both chemisorption and physisorption are existed in adsorption courses. The results show that the anatase TiO2 powders possess the highest chemisorption capacities among the samples, which can be attributed to its higher surface energy, rough surface and unsaturated oxygen bond. The mechanism of adsorption is suggested to be the soft acid/soft base interactions to form complexes for chemisorption and electrostatic force of electric double layer (EDL) model for physisorption.

从案卷移送制度的恢复谈刑事庭前审查程序的完善

謝佑平(Xie Youping),廉曉媚(lian xiaomei) 전북대학교 법학연구소 2012 法學硏究 Vol.37 No.-

The re-modified of criminal procedure law has restored the files transfer system, which is a rational choice based on the reflection of judicial practice. It is conducive to protect the reading rights of defence lawyers, to prevent the abuse the public prosecution power, to save lawsuit cost and to improve the efficiency of the proceedings. However, the preliminary hearing procedure has not been changed correspondingly. If the preliminary hearing procedure’s form is brief, function is simple and could not exclude of judges’ prejudgments, it will not only influence the expected purpose of restoring the files transfer system but also have conflicts. Therefore, the restored of files transfer system requires further reform of preliminary hearing procedure.

Differential Gene Expression in the Pathogenic Strains of Actinobacillus pleuropneumoniae Serotypes 1 and 3

( Fang Xie ),( Ming Jun Zhang ),( Shu Qing Li ),( Chong Tao Du ),( Chang Jiang Sun ),( Wen Yu Han ),( Liang Zhou ),( Lian Cheng Lei ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.4

An Updated Meta-analysis Between the Association of XRCC1 Arg399Gln Polymorphism and Hepatocellular Carcinoma Risk

Zhang, Xiao-Lian,Lu, Yu,Yang, Shi,Peng, Qi-Liu,Wang, Jian,Xie, Li,Deng, Yan,He, Yu,Li, Tai-Jie,Qin, Xue,Li, Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Various studies have evaluated the relationship between X-ray repair cross-complementing group 1 (XRCC1) Arg399Gln polymorphism and hepatocellular carcinoma (HCC) risk, but the conclusions have been inconsistent and underpowered. The purpose of this updated meta-analysis was to examine whether XRCC1 Arg399Gln polymorphism confers susceptibility to HCC. Methods: Eligible studies extracted from PubMed, Embase, Cochrane Library, VIP (chinese) and CNKI (chinese) up to November 2013 were included in the study. Pooled odds ratio (OR) together with their 95% confidence interval (CI) were estimated to evaluate XRCC1 Arg399Gln polymorphism and HCC risk. Results: Finally, 21 studies with 4,170 cases and 5,030 controls were involved in our meta-analysis. The results demonstrated that there was significant association between Arg399Gln polymorphism and HCC risk under two contrast models in overall populations (AG vs GG: OR=1.265, 95%CI=1.036-1.545, p=0.021; AA+AG vs GG: OR=1.240, 95%CI=1.021-1.506, p=0.030). In subgroup analyses, significant association was found in Asians (A vs G: OR=1.175, 95%CI=1.013-1.362, p=0.033; AG vs GG: OR=1.317, 95%CI=1.070-1.622, p=0.009; AA+AG vs GG: OR=1.289, 95%CI=1.055-1.575, p=0.013) and Caucasians (A vs G: OR=0.591, 95%CI=0.361-0.966, p=0.036; AA+AG vs GG: OR=0.468, 95%CI=0.234-0.934, p=0.031). Conclusions: The results suggest that XRCC1 Arg399Gln polymorphism may increase HCC risk especially among Asians. However, XRCC1 Arg399Gln polymorphism might act as a protective role against HCC among Caucasians.

Identification of a Methyltransferase Encoded by Gene ste16 and Its Function in Ebosin Biosynthesis of Streptomyces sp. 139

Hong-Guan Xie,Yong-Gang Bao,Li-ping Bai,Jun-Jie Shan,Rong Jiang,Yang Zhang,Lian-Hong Guo,Ren Zhang,Yuan Li 한국미생물학회 2009 The journal of microbiology Vol.47 No.2

Streptomyces sp. 139 generates a novel exopolysaccharide (EPS) designated as Ebosin, which exerts an antagonistic effect on IL-1R in vitro and anti-rheumatic arthritis activity in vivo. A ste gene cluster for Ebosin biosynthesis consisting of 27 ORFs was previously identified in our laboratory. In this paper, ste16 was expressed in Escherichia coli BL21 and the recombinant protein was purified, which has the ability to catalyze the transfer of the methyl group from S-adenosylmethionine (AdoMet) to dTDP-4-keto-6-deoxy-D-glucos, which was thus identified as a methyltransferase. In order to determine the function of ste16 in Ebosin biosynthesis, the gene was disrupted with a double crossover via homologous recombination. The monosaccharide composition of EPS-m generated by the mutant strain Streptomyces sp. 139 (ste16-) was found to differ from that of Ebosin. The IL-1R antagonist activity of EPS-m was markedly lower than that of Ebosin. These experimental results have shown that the ste16 gene codes for a methyltransferase which is involved in Ebosin biosynthesis.

MiRNA-15a Mediates Cell Cycle Arrest and Potentiates Apoptosis in Breast Cancer Cells by Targeting Synuclein-γ

Li, Ping,Xie, Xiao-Bing,Chen, Qian,Pang, Guo-Lian,Luo, Wan,Tu, Jian-Cheng,Zheng, Fang,Liu, Song-Mei,Han, Lu,Zhang, Jian-Kun,Luo, Xian-Yong,Zhou, Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: Recent studies have indicated that microRNA-15a (miR-15a) is dysregulated in breast cancer (BC). We aimed to evaluate the expression of miR-15a in BC tissues and corresponding para-carcinoma tissues. We also focused on effects of miR-15a on cellular behavior of MDA-MB-231 and expression of its target gene synuclein-${\gamma}$ (SNCG). Materials and Methods: The expression levels of miR-15a were analysed in BC formalin fixed paraffin embedded (FFPE) tissues by microarray and quantitative real-time PCR. CCK-8 assays, cell cycle and apoptosis assays were used to explore the potential functions of miR-15a in MDA-MB-231 human BC cells. A luciferase reporter assay confirmed direct targets. Results: Downregulation of miR-15a was detected in most primary BCs. Ectopic expression of miR-15a promoted proliferation and suppressed apoptosis in vivo. Further studies indicated that miR-15a may directly interact with the 3'-untranslated region (3'-UTR) of SNCG mRNA, downregulating its mRNA and protein expression levels. SNCG expression was negatively correlated with miR-15a expression. Conclusions: MiR-15a has a critical role in mediating cell cycle arrest and promoting cell apoptosis of BC, probably by directly targeting SNCG. Thus, it may be involved in development and progression of BC.