Effect of stocking density and alpha-lipoic acid on the growth performance, physiological and oxidative stress and immune response of broilers

Wenjia Li,Fengxian Wei,Bin Xu,Quanyou Sun,Wen Deng,Huihui Ma,Jie Bai,Shaoyu Li 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: The study was conducted to evaluate the effect of stocking density and alpha-lipoic acid (ALA) on the growth performance, feed utilization, carcass traits, antioxidative ability and immune response of broilers. Methods: A total of 1,530 22-day-old male broilers (Arbor Acres) with comparable body weights (731.92±5.26) were placed into 18 cages (2.46×2.02 m) in groups of 75 birds (15 birds/m2, 37.5 kg/m2; low stocking density [LD]), 90 birds (18 birds/m2, 45.0 kg/m2; high stocking density [HD]) and 90 birds with 300 mg/kg ALA added to the basal diet (18 birds/m2, 45.0 kg/m2; HD+ALA, high stocking density+α-lipoic acid); each treatment was represented by 6 replicates. The experimental period was 3 weeks. Results: The results showed that the high stocking density regimen resulted in a decreased growth, feed conversion ratio, carcass weight, thigh yield and bursa weight relative to body weight (p<0.05) on d 42. The abdominal fat yield in the HD+ALA group was lower (p = 0.031) than that of the LD group at 42 d. The superoxide dismutase and glutathione peroxidase activities in serum were increased, and malondialdehyde content decreased after adding ALA product (p<0.05) on d 42. Additionally, the serum concentrations of immunoglobulin A (IgA) and IgG were decreased (p<0.05) and the level of diamine oxidase was higher (p< 0.01) in the HD group on d 42. Conclusion: The high stocking density significantly decreased broiler growth performance, feed utilization and carcass traits, increased physiological and oxidative stress and induced intestinal mucosal injury. The supplementation of ALA product in broiler diet at 300 mg/kg may reduce the adverse effects of high stocking density-mediated stress by maintaining the antioxidant system and humoral immune system.

Function of Rhs proteins in porcine extraintestinal pathogenic Escherichia coli PCN033

Lu Wenjia,Tan Jia,Lu Hao,Wang Gaoyan,Dong Wenqi,Wang Chenchen,Li Xiaodan,Tan Chen 한국미생물학회 2021 The journal of microbiology Vol.59 No.9

Extraintestinal pathogenic Escherichia coli (ExPEC) is an important zoonotic pathogen that places severe burdens on public health and animal husbandry. There are many pathogenic factors in E. coli. The type VI secretion system (T6SS) is a nano-microbial weapon that can assemble quickly and inject toxic effectors into recipient cells when danger is encountered. T6SSs are encoded in the genomes of approximately 25% of sequenced Gram-negative bacteria. When these bacteria come into contact with eukaryotic cells or prokaryotic microbes, the T6SS assembles and secretes associated effectors. In the porcine ExPEC strain PCN033, we identified four classic rearrangement hotspot (Rhs) genes. We determined the functions of the four Rhs proteins through mutant construction and protein expression. Animal infection experiments showed that the Δrhs-1CT, Δrhs-2CT, Δrhs-3CT, and Δrhs-4CT caused a significant decrease in the multiplication ability of PCN033 in vivo. Cell infection experiments showed that the Rhs protein is involved in anti-phagocytosis activities and bacterial adhesion and invasion abilities. The results of this study demonstrated that rhs1, rhs3, and rh4 plays an important role in the interaction between PCN033 and host cell. Rhs2 has contribution to cell and mice infection. This study helps to elucidate the pathogenic mechanism governing PCN033 and may help to establish a foundation for further research seeking to identify potential T6SS effectors.

Erratum - The effect of various sandblasting conditions on surface changes of dental zirconia and shear bond strength between zirconia core and indirect composite resin

Naichuan Su,Li Yue,Yunmao Liao,Wenjia Liu,Hai Zhang,Xin Li,Hang Wang,Jiefei Shen 대한치과보철학회 2015 The Journal of Advanced Prosthodontics Vol.7 No.6

Evolution of Mechanical Properties of Ti–6Al–4V Alloy in the Temperature Range of 20 to − 196 °C

Wei Zhao,Wenjia Su,Liang Li,Ding Fang,Ni Chen 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9

Cryogenic cooling is becoming an attractive method for machining difficult-to-cut materials to extend their machinability. However, it’s a challenging task to analyze the effect of cryogenic cooling on the machining. In order to better understandthe various physical phenomena in the cryogenic cutting process, cryogenic impact test, and tensile test of titanium alloyTi–6Al–4V were undertaken in this work. The impact and tensile properties of Ti–6Al–4V alloy in the temperature range of20 to − 196 °C were investigated. Later on, the macroscopic and microscopic morphology of the produced fractures wereanalyzed. Findings have depicted a decreasing impact toughness and an increase of tensile and yield strength of Ti–6Al–4Valloy in the temperature range from 20 to − 196 °C. The impact fracture appeared as a tendency of becoming material brittle. Meanwhile, three kinds of zones of shear-lip, fiber, and radiation were found on the fracture morphology. Those zonescorrespond to the shear fracture zone, crack initiation zone, and crack extension zone, respectively. With the temperaturedecrease, the proportion of fiber zone was decreased, while a gradual increase in the radiation zone was observed. Besides,the fiber zone was observed for the macroscopic morphology of the fracture under the temperature of − 196 °C. Somedimples were found in the tensile fracture at − 196 °C indicating the ability of Ti–6Al–4V alloy to deform plastically undersuch low temperature. This work is a fundamental support to analyze the behavior of Ti–6Al–4V alloy under cryogeniccooling conditions.

Optimized Iterative Algorithm for Energy-Efficient Power Allocation in Two-tier Heterogeneous Networks

Wang Junliang,Li Wenjia,Liu Haitao 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.12

In heterogeneous networks, the issue of interference between femtocells and macrocells should be carefully considered. Resource allocation schemes with cognitive technologies have been a key challenge to manage interference. In this paper, we investigate price-based power allocation strategies with the energy efficiency criterion for a spectrum-sharing heterogeneous cognitive network from the aspect of energy efficiency, and provide the utility function of macrocell and femtocells based on a non-cooperative Stackleberg game model. We build a combination of price vector and power allocation values by standard Lagrangian method and propose an improved iteration algorithm based on price updating to obtain the Stackleberg equilibrium solution. The simulation results verify the proposed method can improve energy efficiency and achieve better utility.

The effect of various sandblasting conditions on surface changes of dental zirconia and shear bond strength between zirconia core and indirect composite resin

Su, Naichuan,Yue, Li,Liao, Yunmao,Liu, Wenjia,Zhang, Hai,Li, Xin,Wang, Hang,Shen, Jiefei The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.3

PURPOSE. To measure the surface loss of dental restorative zirconia and the short-term bond strength between an indirect composite resin (ICR) and zirconia ceramic after various sandblasting processes. MATERIALS AND METHODS. Three hundred zirconia bars were randomly divided into 25 groups according to the type of sandblasting performed with pressures of 0.1, 0.2, 0.4 and 0.6 MPa, sandblasting times of 7, 14 and 21 seconds, and alumina powder sizes of 50 and $110{\mu}m$. The control group did not receive sandblasting. The volume loss and height loss on zirconia surface after sandblasting and the shear bond strength (SBS) between the sandblasted zirconia and ICR after 24-h immersion were measured for each group using multivariate analysis of variance (ANOVA) and Least Significance Difference (LSD) test (${\alpha}$=.05). After sandblasting, the failure modes of the ICR/zirconia surfaces were observed using scanning electron microscopy. RESULTS. The volume loss and height loss were increased with higher sandblasting pressure and longer sandblasting treatment, but they decreased with larger powder size. SBS was significantly increased by increasing the sandblasting time from 7 seconds to 14 seconds and from 14 seconds to 21 seconds, as well as increasing the size of alumina powder from $50{\mu}m$ to $110{\mu}m$. SBS was significantly increased from 0.1 MPa to 0.2 MPa according to the size of alumina powder. However, the SBSs were not significantly different with the sandblasting pressure of 0.2, 0.4 and 0.6 MPa. The possibilities of the combination of both adhesive failure and cohesive failure within the ICR were higher with the increases in bonding strength. CONCLUSION. Based on the findings of this study, sandblasting with alumina particles at 0.2 MPa, 21 seconds and the powder size of $110{\mu}m$ is recommended for dental applications to improve the bonding between zirconia core and ICR.

The effect of various sandblasting conditions on surface changes of dental zirconia and shear bond strength between zirconia core and indirect composite resin

Jiefei Shen,Xin Li,Naichuan Su,Wenjia Liu,Yunmao Liao,Li Yue,Hang Wang,Hai Zhang 대한치과보철학회 2015 The Journal of Advanced Prosthodontics Vol.7 No.3

PURPOSE To measure the surface loss of dental restorative zirconia and the short-term bond strength between an indirect composite resin (ICR) and zirconia ceramic after various sandblasting processes. MATERIALS AND METHODS Three hundred zirconia bars were randomly divided into 25 groups according to the type of sandblasting performed with pressures of 0.1, 0.2, 0.4 and 0.6 MPa, sandblasting times of 7, 14 and 21 seconds, and alumina powder sizes of 50 and 110 µm. The control group did not receive sandblasting. The volume loss and height loss on zirconia surface after sandblasting and the shear bond strength (SBS) between the sandblasted zirconia and ICR after 24-h immersion were measured for each group using multivariate analysis of variance (ANOVA) and Least Significance Difference (LSD) test (α=.05). After sandblasting, the failure modes of the ICR/zirconia surfaces were observed using scanning electron microscopy. RESULTS The volume loss and height loss were increased with higher sandblasting pressure and longer sandblasting treatment, but they decreased with larger powder size. SBS was significantly increased by increasing the sandblasting time from 7 seconds to 14 seconds and from 14 seconds to 21 seconds, as well as increasing the size of alumina powder from 50 µm to 110 µm. SBS was significantly increased from 0.1 MPa to 0.2 MPa according to the size of alumina powder. However, the SBSs were not significantly different with the sandblasting pressure of 0.2, 0.4 and 0.6 MPa. The possibilities of the combination of both adhesive failure and cohesive failure within the ICR were higher with the increases in bonding strength. CONCLUSION Based on the findings of this study, sandblasting with alumina particles at 0.2 MPa, 21 seconds and the powder size of 110 µm is recommended for dental applications to improve the bonding between zirconia core and ICR.

De-cloaking Malicious Activities in Smartphones Using HTTP Flow Mining

( Xin Su ),( Xuchong Liu ),( Jiuchuang Lin ),( Shiming He ),( Zhangjie Fu ),( Wenjia Li ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.6

Android malware steals users` private information, and embedded unsafe advertisement (ad) libraries, which execute unsafe code causing damage to users. The majority of such traffic is HTTP and is mixed with other normal traffic, which makes the detection of malware and unsafe ad libraries a challenging problem. To address this problem, this work describes a novel HTTP traffic flow mining approach to detect and categorize Android malware and unsafe ad library. This work designed AndroCollector, which can automatically execute the Android application (app) and collect the network traffic traces. From these traces, this work extracts HTTP traffic features along three important dimensions: quantitative, timing, and semantic and use these features for characterizing malware and unsafe ad libraries. Based on these HTTP traffic features, this work describes a supervised classification scheme for detecting malware and unsafe ad libraries. In addition, to help network operators, this work describes a fine-grained categorization method by generating fingerprints from HTTP request methods for each malware family and unsafe ad libraries. This work evaluated the scheme using HTTP traffic traces collected from 10778 Android apps. The experimental results show that the scheme can detect malware with 97% accuracy and unsafe ad libraries with 95% accuracy when tested on the popular third-party Android markets.

PREPARATION OF FOLATE-CONJUGATED BOVINE SERUM ALBUMIN NANOPARTICLES ADSORBING EPIRUBICIN HYDROCHLORIDE

XIUHUA ZHAO,JIAMING TANG,YUANGANG ZU,WEIGUO WANG,NA LI,WENJIA XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

This work investigated the preparation process of folate-conjugated bovine serum albumin nanoparticles (FA–BSANPs) adsorbing epirubicin hydrochloride (EPI) nanoparticles (FA–EPI–BSANPs), a specific-targeting drug delivery system in cancer chemotherapy. The BSANPs were prepared by desolvation as a drug carrier system and conjugated with folate to produce FA–EPI–BSANPs that specifically target tumors by cross-linking. EPI, an anticancer drug, was adsorbed by this drug carrier system. The influences of six experimental parameters, namely, the adsorption time, FA–BSANPs solution-adsorbed EPI concentration, stirring speed, FA–BSANPs solution pH, the ratio of glutaraldehyde and BSA, and mass ratio of FA–BSANPs to EPI, on the drug loading efficiency (DLR) and drug entrapment efficiency (DER) of FA–EPI–BSANPs were investigated via the single factor method. The results indicated that the optimum operation conditions were obtained with 145.4 nm±0.5 nm MPS, 23.41% DLR and 98.93% DER. The N-hydroxysuccinimide-folate content associated with BSANPs was up to 0.9757% (wt). The DLR and DER of EPI increased with increasing adsorption time, FA–BSANPs solution concentration, and pH value, peaking at 1750 rpm with increasing stirring speed, but decreasing thereafter. The FA–EPI–BSANPs obtained were characterized by laser light scattering, scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and thermogravimetric analysis. Drug release in vitro was investigated, as well. The characterization results showed that EPI in FA–EPI–BSANPs existed in an amorphous, instead of crystalline state. Most of the EPI was enclosed by FA–BSANPs, and a small amount was adsorbed onto the surface of the FA–BSANPs. The FA–EPI–BSANPs particles are nearly ellipsoidal and significantly affect sustained release. The inhibitory rate of FA–EPI–BSANP was mensurated by MTT method. The inhibitory rate of FA–EPI–BSANPs for SMMC 7721 cell developed with raise of concentration and was higher than other samples. The IC50 values of FA–EPI–BSANPs and EPI were 11.5 μg/mL and 18.8 μg/mL, respectively. The target ability of FA–EPI–BSANP for SMMC 7721 cell was mensurated by fluorescence (FITC) modified albumin techniques. The uptake rate of FA–EPI–BSANPs was higher than samples without folate conjugated, and increased with increased concentration.

The association between obesity and glaucoma in older adults: evidence from the China Health and Retirement Longitudinal Study

Zhao Xiaohuan,Bo Qiyu,Sun Junran,Chen Jieqiong,Li Tong,Huang Xiaoxu,Zhou Minwen,Wang Jing,Liu Wenjia,Sun Xiaodong 한국역학회 2023 Epidemiology and Health Vol.45 No.-

Objectives: This study evaluated the association between obesity and glaucoma in middle-aged and older people. A population-based retrospective cohort study was conducted using data from the China Health and Retirement Longitudinal Study.Methods: Glaucoma was assessed via self-reports. Multivariate logistic regression analysis and a Cox proportional hazards model were used to assess the relationship between obesity and glaucoma risk.Results: Older men living in urban areas who were single, smokers, and non-drinkers were found to have a significantly higher incidence of glaucoma (all P<0.05). Diabetes, hypertension, and kidney disease were also associated with higher glaucoma risk, while dyslipidemia was associated with lower risk (all P<0.05). After the model was adjusted for demographic, socio-economic, and health-related variables, obesity was significantly associated with a 10.2% decrease in glaucoma risk according to the Cox proportional hazards model (hazard ratio, 0.898; 95% CI, 0.829-0.973) and an 11.8% risk reduction in the multivariate logistic regression analysis (odds ratio, 0.882; 95% CI, 0.803-0.968). A further subgroup analysis showed that obesity was associated with a reduced risk of glaucoma in people living in rural areas, in smokers, and in those with kidney disease (all P<0.05). Obesity also reduced glaucoma risk in people with diabetes, hypertension, or dyslipidemia more than in healthy controls (all P<0.05).Conclusions: This cohort study suggests that obesity was associated with a reduced risk of glaucoma, especially in rural residents, smokers, and people with kidney disease. Obesity exerted a stronger protective effect in people with diabetes, hypertension, or dyslipidemia than in healthy people.