Efficient Mucosal Immunization by Mucoadhesive and pH-Sensitive Polymeric Vaccine Delivery System

Lei Xing,Tian-Jiao Zhou,Ya-Tong Fan,Yu-jing He,Tao Pang,조기현,Jinjian Lu,Hu-Lin Jiang,조종수 한국고분자학회 2019 Macromolecular Research Vol.27 No.3

Mucosal surfaces as the largest immune organ of human body cover 400 m2 of the body including the gastrointestinal, urogenital, and respiratory tracts. The local mucosal immunity is an important first line of defense against many pathogens because most pathogens initiate their infection through access to the mucosal region of body. Also, the mucosal vaccines induce mucosal and systemic immunity simultaneously. Therefore, attracted by the advantages of mucosal immunity, researchers keep an eye on the mucoadhesive and pH-sensitive polymeric vaccine delivery system to solve several limitations of mucosal administration, making mucosal immunity receive great interests lately. In this review, we discuss natural polymer- and synthetic polymer-based mucoadhesive and pHsensitive systems used for mucosal vaccine delivery. Also, we cover how to target antigen presenting cells and M cells for the cell specificity. Finally, we conclude the significant progress in mucosal vaccine and the prospect mucosal vaccine research in the future.

Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis

Lei Cheng,Jianjun Chen,Qingling Fu,Shaoheng He,Huabin Li,Zheng Liu,Guolin Tan,Zezhang Tao,Dehui Wang,Weiping Wen,Rui Xu,Yu Xu,Qintai Yang,Chonghua Zhang,Gehua Zhang,Ruxin Zhang,Yuan Zhang,Bing Zhou,Do 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Allergic rhinitis (AR) is a global health problem that causes major illnesses and disabilities worldwide. Epidemiologic studies have demonstrated that the prevalence of AR has increased progressively over the last few decades in more developed countries and currently affects up to 40% of the population worldwide. Likewise, a rising trend of AR has also been observed over the last 2-3 decades in developing countries including China, with the prevalence of AR varying widely in these countries. A survey of self-reported AR over a 6-year period in the general Chinese adult population reported that the standardized prevalence of adult AR increased from 11.1% in 2005 to 17.6% in 2011. An increasing number of original articles and imporclinical trials on the epidemiology, pathophysiologic mechanisms, diagnosis, management and comorbidities of AR in Chinese subjects have been published in international peer-reviewed journals over the past 2 decades, and substantially added to our understanding of this disease as a global problem. Although guidelines for the diagnosis and treatment of AR in Chinese subjects have also been published, they have not been translated into English and therefore not generally accessible for reference to non-Chinese speaking international medical communities. Moreover, methods for the diagnosis and treatment of AR in China have not been standardized entirely and some patients are still treated according to regional preferences. Thus, the present guidelines have been developed by the Chinese Society of Allergy to be accessible to both national and international medical communities involved in the management of AR patients. These guidelines have been prepared in line with existing international guidelines to provide evidence-based recommendations for the diagnosis and management of AR in China.

A Discrete State-Space Control Scheme for Dynamic Voltage Restorers

He Lei,Xin-Chun Lin,Ming-Yu Xue,Yong Kang 전력전자학회 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.3

This paper presents a discrete state-space controller using state feedback control and feed-forward decoupling to provide a desirable control bandwidth and control stability for dynamic voltage restorers (DVR). The paper initially discusses three typical applications of a DVR. The load-side capacitor DVR topology is preferred because of its better filtering capability. The proposed DVR controller offers almost full controllability because of the multi-feedback of state variables, including one-beat delay feedback. Feed-forward decoupling is usually employed to prevent disturbances of the load current and source voltage. Directly obtaining the feed-forward paths of the load current and source voltage in the discrete domain is a complicated process. Fortunately, the full feed-forward decoupling strategy can be easily applied to the discrete state-space controller by means of continuous transformation. Simulation and experimental results from a digital signal processor-based system are included to support theoretical analysis.

A Discrete State-Space Control Scheme for Dynamic Voltage Restorers

Lei, He,Lin, Xin-Chun,Xue, Ming-Yu,Kang, Yong The Korean Institute of Power Electronics 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.3

This paper presents a discrete state-space controller using state feedback control and feed-forward decoupling to provide a desirable control bandwidth and control stability for dynamic voltage restorers (DVR). The paper initially discusses three typical applications of a DVR. The load-side capacitor DVR topology is preferred because of its better filtering capability. The proposed DVR controller offers almost full controllability because of the multi-feedback of state variables, including one-beat delay feedback. Feed-forward decoupling is usually employed to prevent disturbances of the load current and source voltage. Directly obtaining the feed-forward paths of the load current and source voltage in the discrete domain is a complicated process. Fortunately, the full feed-forward decoupling strategy can be easily applied to the discrete state-space controller by means of continuous transformation. Simulation and experimental results from a digital signal processor-based system are included to support theoretical analysis.

Age-stage, two-sex life tables of the maple poison moth (Lymantria nebulosa Wileman) at different ambient temperatures

Lei Ling,Huang Hui Jing,Yi Jin Yu,Qiu Lin,Ding Wen Bin,He Hua Liang,Yi Tu Yong,Li You Zhi,Xue Jin 한국곤충학회 2021 Entomological Research Vol.51 No.4

The maple poison moth (MPM), Lymantria nebulosa (Wileman), is a Lepidopteran defoliator and one of the most important pests of Liquidambar formosana Hance. The objective of this study was to determine the effects of temperature on the population growth and reproduction of this species. MPM were raised in artificial climate chambers in a laboratory at 21 ± 1°C, 24 ± 1°C, 27 ± 1°C, 30 ± 1°C, 33 ± 1°C and 36 ± 1°C, under a relative humidity (RH) of 70% ± 10% and a 14 L:10D photoperiod, and age-stage, two-sex life tables complied for insects at each temperature. The developmental duration, adult preoviposition period and overall preoviposition period decreased with temperature in a time-dependent manner. The growth curves of the different temperature groups overlapped considerably. The highest larval survival rate (80%) was recorded at 30 ± 1°C. At 36°C only a few eggs hatched and the first instar could not complete the first molt. We consequently regard 36°C as the lethal temperature threshold for this species. Life expectancy decreased with age under all six temperatures. The endogenous growth rate was significantly lower at 21 ± 1°C than at the other temperatures, and was highest in 30 ± 1°C group. The net growth rate of the 30 ± 1°C group was also significantly higher than others. Reproductive parameters were highest at 27 ± 1°C and 30 ± 1°C. Although environmental factors absent from the laboratory environment may limit the generality of our results, they nonetheless provide a theoretical basis for developing integrated control methods for MPM in the wild.

Effects of different carbon sources on the phase composition and microstructure of synthesized SiC-B4C composite powders

Yu Cao,Ruyi Deng,Jilin Hu,Jinxiu He,Dapeng Lei,Zhanjun Chen,Yangxi Peng 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2

SiC-B4C composite powders were synthesized by the carbothermal reduction method under an argon atmosphere usingdifferent kinds of carbon sources (carbon black and starch) and silica sol and boric acid as the precursor raw materials. Basedon thermodynamic analysis and calculation, the effects of different carbon sources and reaction temperatures on the mass lossrate, phase composition, and microstructure of SiC-B4C ultrafine composite powders were comparatively studied. Resultsshowed that the optimum conditions for synthesizing SiC-B4C composite powders with carbon black as the carbon source were1550 ºC for 2 h, whereas the optimum conditions for synthesizing SiC-B4C composite powders with starch as the carbon sourcewere 1450 ºC-1550 ºC for 2 h. The powder samples synthesized with carbon black as the carbon source at 1550 ºC were mainlycomposed of flaky, columnar-like, spherical, and irregular polyhedral particles (about 100-200 nm in diameter). Mutualcohesion or agglomeration between particles was minimal. In the powder samples synthesized at 1550 ºC with an excess of 10wt% starch, in addition to a certain amount of flaky, spherical, and other irregular structure particles, a certain amount ofuniform, slender whiskers (about 50-100 nm in diameter) and a certain phenomenon of lap and winding between the whiskerswere noted. The powder samples synthesized at 1550 ºC with an excess of 20 wt% starch had no whisker-like substance.

Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells

Yu-qi He,Jian-qiu Sheng,Xian-long Ling,Lei Fu,Peng Jin,Lawrence Yen,Jianyu Rao 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.12

Estrogen has anti-colorectal cancer effects which are thought to be mediated by mismatch repair gene (MMR) activity. Estrogen receptor (ER) expression is associated with microRNA (miRNA) expression in ER-positive tumors. However, studies of direct link between estrogen (especially estradiol E2), miRNA expression, and MMR in colorectal cancer (CRC) have not been done. In this study, we first evaluated the effects of estradiol (E2) and its antagonist ICI182,780 on the expression of miRNAs (miR-31, miR-155 and miR-135b) using COLO205, SW480 and MCF-7 cell lines, followed by examining the association of tissue miRNA expression and serum E2 levels using samples collected from 18 colorectal cancer patients. E2inhibited the expressions of miRNAs in COLO205 cells,which could be reversed by E2 antagonist ICI 182.780. The expression of miR-135b was inversely correlated with serum E2 level and ER-β mRNA expression in CRC patients’ cancer tissues. There were significant correlations between serum E2 level and expression of ER-β, miR-135b, and MMR in colon cancer tissue. This study suggests that the effects of estrogen on MMR function may be related to regulating miRNA expression via ER-β, which may be the basis for the anti-cancer effect in colorectal cells.

Spatial Regionalization on Surface Ozone in the Yangtze River Delta of China

Lei Tong,Hang Xiao,Hui Yi,Yu Liu,Jie Zheng,Cenyan Huang,Mengmeng He 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.2

In order to better understand the tempo-spatial characteristics of surface ozone (O3) on the regional scale of Yangtze River Delta (YRD), China, cluster analysis was applied to surface O3 monitored from 2016 to 2017 at 110 state-controlled stations. Eight spatial regions with distinct patterns of O3 variations were identified. The exceedances over the Grade I (100 μg/m3) andGrade II (160μg/m3) National Ambient Air Quality Standard of maximum daily average 8 h O3 (MDA8 O3) varied within 99~192 and 1~41 days, respectively, for different subregions. The central and east YRD were the most polluted subregions with higher incidence of O3 episodes, while the southwest and southeast YRD were relatively clean. The spatial disparity of O3 over YRD was strongly affected by local emissions, with larger precursor emissions contributing to higher frequencies of O3 pollution and larger amplitudes of temporal variations. The regionalO3 pollution mainly occurred in spring, with higher peaks ofMDA8O3 being observed duringApril ~May for most subregions. While for east YRD, O3 pollution was most serious in summer, when its monthly MDA8 O3 reached the highest value (141.0 μg/m3). During thewhole study period, the spatial differences of weekly/monthly amplitudes ofMDA8O3 were similar to those of NO2 among the eight subregions, indicating the significant influence of NO2 on O3 over YRD on the yearly timescale. This influence was especially evident in warm seasons for the coastal subregions, where O3 production was controlled by NOx. While for the west inland subregion, O3was less affected by NO2 variation, indicating a NOx-saturated characteristic of O3 formation.

Branched polymeric prodrug/programmed cell death 4 complexes for combinational cancer therapy

Yu‑Jing He,Lei Xing,Peng‑Fei Cui,Jia‑Liang Zhang,Jian‑Bin Qiao,Cheng‑Qiong Luo,Ge Jiang,Hu‑Lin Jiang 한국약제학회 2017 Journal of Pharmaceutical Investigation Vol.47 No.2

Here, we demonstrate a co-delivery system constructed by integrating chemotherapeutic molecules into branched polymeric prodrug which can condense nucleic acids. Demethylcantharidate (DCA) was chosen as a model drug and premodified through nucleophilic substitution reaction by its two carboxylic groups with allyl chloride. The synthesized intermediate (DCA-dially) was then used to polymerize with tris (2-aminoethyl) amine (TAEA) through progressively ammonolysis reaction. The obtained poly (DCA-alt-TAEA) (DCAT) was used to pack PDCD4 into spherical-like nanoparticles through electrostatic interaction. Gel retardation assays implied that DCAT could integrate DNA at the weight ratio of 1 and protect it from digestion by nuclease. Acid-base titration experiments showed that DCAT obtained preferable buffering capability which was beneficial for the endosomal escape of DCAT/PDCD4 complexes. Cellular tests involving gene transfection efficiency and cytotoxicity indicated that DCA and PDCD4 co-delivered by the complexes significantly and synergistically suppressed the viability of SMMC-7721 cells. These results suggest that integrating chemotherapeutic molecules into nucleic acid-packing polymeric prodrug as cationic polymer/PDCD4 complexes is a highly efficient way to co-deliver chemotherapeutic drugs and nucleic acids for cancer therapy.

Radiation shielding optimization design research based on bare-bones particle swarm optimization algorithm

Jichong Lei,Chao Yang,Huajian Zhang,Chengwei Liu,Dapeng Yan,Guanfei Xiao,Zhen He,Zhenping Chen,Tao Yu Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

In order to further meet the requirements of weight, volume, and dose minimization for new nuclear energy devices, the bare-bones multi-objective particle swarm optimization algorithm is used to automatically and iteratively optimize the design parameters of radiation shielding system material, thickness, and structure. The radiation shielding optimization program based on the bare-bones particle swarm optimization algorithm is developed and coupled into the reactor radiation shielding multi-objective intelligent optimization platform, and the code is verified by using the Savannah benchmark model. The material type and thickness of Savannah model were optimized by using the BBMOPSO algorithm to call the dose calculation code, the integrated optimized data showed that the weight decreased by 78.77%, the volume decreased by 23.10% and the dose rate decreased by 72.41% compared with the initial solution. The results show that the method can get the best radiation shielding solution that meets a lot of different goals. This shows that the method is both effective and feasible, and it makes up for the lack of manual optimization.