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RISS 인기검색어
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- 저자 : Jinxiu He (검색결과 4 건)
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Diversity and Antifungal Activity of Endophytic Fungi Associated with Camellia oleifera
( Jinxiu Yu ),( Ying Wu ),( Zhen He ),( Mi Li ),( Kaiming Zhu ),( Bida Gao ) 한국균학회 2018 Mycobiology Vol.46 No.2
Endophytic fungi strains (n¼81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson’s and Shannon’s diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.
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Yu Cao,Ruyi Deng,Jilin Hu,Jinxiu He,Dapeng Lei,Zhanjun Chen,Yangxi Peng 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2
SiC-B4C composite powders were synthesized by the carbothermal reduction method under an argon atmosphere usingdifferent kinds of carbon sources (carbon black and starch) and silica sol and boric acid as the precursor raw materials. Basedon thermodynamic analysis and calculation, the effects of different carbon sources and reaction temperatures on the mass lossrate, phase composition, and microstructure of SiC-B4C ultrafine composite powders were comparatively studied. Resultsshowed that the optimum conditions for synthesizing SiC-B4C composite powders with carbon black as the carbon source were1550 ºC for 2 h, whereas the optimum conditions for synthesizing SiC-B4C composite powders with starch as the carbon sourcewere 1450 ºC-1550 ºC for 2 h. The powder samples synthesized with carbon black as the carbon source at 1550 ºC were mainlycomposed of flaky, columnar-like, spherical, and irregular polyhedral particles (about 100-200 nm in diameter). Mutualcohesion or agglomeration between particles was minimal. In the powder samples synthesized at 1550 ºC with an excess of 10wt% starch, in addition to a certain amount of flaky, spherical, and other irregular structure particles, a certain amount ofuniform, slender whiskers (about 50-100 nm in diameter) and a certain phenomenon of lap and winding between the whiskerswere noted. The powder samples synthesized at 1550 ºC with an excess of 20 wt% starch had no whisker-like substance.
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Xia Wu,Xiaoyan Chi,Yanhua Wang,Kailu Zhang,Le Kai,Qiuning He,Jinxiu Tang,Kewen Wang,Longshuo Sun,Xiuying Hao,Weihai Xie,Yihe Ge 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.4
In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role in suppression of mycelial growth of some fungal pathogens that cause plant diseases in agriculture. Although some regulators for pyrrolnitrin biosynthesis were identified, the pyrrolnitrin regulation pathway was not fully constructed. During our screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant G05ΔphzΔprn::lacZ and E. coli S17- 1 (pUT/mini-Tn5Kan). By cloning and sequencing of the transposon-flanking DNA fragment, we found that a vfr gene in the conjugant G05W02 was disrupted with mini-Tn5Kan. In one other previous study on P. fluorescens, however, it was reported that the deletion of the vfr caused increased production of pyrrolnitrin and other antifungal metabolites. To confirm its regulatory function, we constructed the vfr-knockout mutant G05Δvfr and G05ΔphzΔprn::lacZΔvfr. By quantifying β-galactosidase activities, we found that deletion of the vfr decreased the prn operon expression dramatically. Meanwhile, by quantifying pyrrolnitrin production in the mutant G05Δvfr, we found that deficiency of the Vfr caused decreased pyrrolnitrin production. However, production of phenazine-1-carboxylic acid was same to that in the wild-type strain G05. Taken together, Vfr is required for pyrrolnitrin but not for phenazine-1-carboxylic acid biosynthesis in P. chlororaphis G05.
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Wu, Xia,Chi, Xiaoyan,Wang, Yanhua,Zhang, Kailu,Kai, Le,He, Qiuning,Tang, Jinxiu,Wang, Kewen,Sun, Longshuo,Hao, Xiuying,Xie, Weihai,Ge, Yihe The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.4
In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role in suppression of mycelial growth of some fungal pathogens that cause plant diseases in agriculture. Although some regulators for pyrrolnitrin biosynthesis were identified, the pyrrolnitrin regulation pathway was not fully constructed. During our screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant $G05{\Delta}phz{\Delta}prn::lacZ$ and E. coli S17-1 (pUT/mini-Tn5Kan). By cloning and sequencing of the transposon-flanking DNA fragment, we found that a vfr gene in the conjugant G05W02 was disrupted with mini-Tn5Kan. In one other previous study on P. fluorescens, however, it was reported that the deletion of the vfr caused increased production of pyrrolnitrin and other antifungal metabolites. To confirm its regulatory function, we constructed the vfr-knockout mutant $G05{\Delta}vfr$ and $G05{\Delta}phz{\Delta}prn::lacZ{\Delta}vfr$. By quantifying ${\beta}-galactosidase$ activities, we found that deletion of the vfr decreased the prn operon expression dramatically. Meanwhile, by quantifying pyrrolnitrin production in the mutant $G05{\Delta}vfr$, we found that deficiency of the Vfr caused decreased pyrrolnitrin production. However, production of phenazine-1-carboxylic acid was same to that in the wild-type strain G05. Taken together, Vfr is required for pyrrolnitrin but not for phenazine-1-carboxylic acid biosynthesis in P. chlororaphis G05.
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