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RISS 인기검색어
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- 저자 : Jinjian Lu (검색결과 3 건)
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Qian Ding,Jiaolin Bao,Wenwen Zhao,Jinjian Lu,Hong Zhu,Xiuping Chen,Xiuping Chen 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.1
Ethanol is a common risk factor for liver injury. Cucurbitacin B (CuB) is a natural product with potent cytotoxic activities mediated by inducing apoptosis. This study investigated the effect of ethanol on CuB-induced cytotoxicity in LO2 hepatocytes. Low concentration of ethanol alone showed no significant cytotoxic effect on LO2 cells. CuB dose-dependently decreased cell viability. However, ethanol co-treatment significantly enhanced CuB-induced cytotoxicity. CuB-induced mitochondria membrane potential (ΔΨ) depolarization was further decreased by ethanol. Furthermore, CuB-induced apoptosis was augmented by ethanol, as evidenced by DNA fragmentation, Annexin V staining, and apoptotic protein expression. Ethanol inhibited CuB-induced autophagy, as determined by MDC staining, autophagic protein expression, and transmission electron microscopy. Therefore, the present data suggested that ethanol enhanced CuB cytotoxicity in LO2 hepatocytes, which was mediated by inhibiting autophagy and augmenting apoptosis.
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Xuenong Zhang,Weiwei Luo,Wenwen Zhao,Jinjian Lu,Xiuping Chen 한국유방암학회 2015 Journal of breast cancer Vol.18 No.2
Purpose: Isocryptotanshinone (ICTS) is a natural bioactive product that is isolated from the roots of the widely used medical herb Salvia miltiorrhiza. However, few reports exist on the mechanisms underlying the therapeutic effects of ICTS. Here, we report that ICTS has anticancer activity and describe the mechanism underlying this effect. Methods: The antiproliferative effect of ICTS was determined using 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2-H-tetrazolium bromide (MTT) and clonogenic assays. The effect of ICTS on the cell cycle was measured using flow cytometry. Apoptosis was determined by Hoechst 33342 staining, DNA fragmentation assays, and Western blotting for apoptotic proteins. Finally, the effect of ICTS on mitogen-activated protein kinases (MAPKs) was determined by Western blotting. Results: ICTS significantly inhibited proliferation of MCF-7 and MDA-MB-231 human breast cancer cells, HepG2 human liver cancer cells, and A549 human lung cancer cells in vitro. Among the tested cell lines, MCF-7 cells showed the highest sensitivity to ICTS. ICTS significantly inhibited colony formation by MCF-7 cells. Furthermore, exposure of MCF-7 cells to ICTS induced cell cycle arrest at the G1 phase and decreased mitochondrial membrane potential. Hoechst 33342 staining and Western blot analysis for apoptotic proteins suggested that ICTS induced apoptosis in MCF-7 cells. In addition, ICTS activated MAPK signaling in MCF-7 cells by inducing time- and concentration-dependent phosphorylation of JNK, ERK, and p38 MAPK. Conclusion: Our results suggest that ICTS inhibited MCF-7 cell proliferation by inducing apoptosis and activating MAPK signaling pathways.
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Efficient Mucosal Immunization by Mucoadhesive and pH-Sensitive Polymeric Vaccine Delivery System
Lei Xing,Tian-Jiao Zhou,Ya-Tong Fan,Yu-jing He,Tao Pang,조기현,Jinjian Lu,Hu-Lin Jiang,조종수 한국고분자학회 2019 Macromolecular Research Vol.27 No.3
Mucosal surfaces as the largest immune organ of human body cover 400 m2 of the body including the gastrointestinal, urogenital, and respiratory tracts. The local mucosal immunity is an important first line of defense against many pathogens because most pathogens initiate their infection through access to the mucosal region of body. Also, the mucosal vaccines induce mucosal and systemic immunity simultaneously. Therefore, attracted by the advantages of mucosal immunity, researchers keep an eye on the mucoadhesive and pH-sensitive polymeric vaccine delivery system to solve several limitations of mucosal administration, making mucosal immunity receive great interests lately. In this review, we discuss natural polymer- and synthetic polymer-based mucoadhesive and pHsensitive systems used for mucosal vaccine delivery. Also, we cover how to target antigen presenting cells and M cells for the cell specificity. Finally, we conclude the significant progress in mucosal vaccine and the prospect mucosal vaccine research in the future.
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