cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

洞長의 名稱·地位·役割에 關한 一硏究

李南久 경북대학교 1985 새마을 硏究論叢 Vol.5 No.-

연폭로여성근로자들에서의 생물학적 연폭로 지표들의 상호관계

남택승,한구웅,김형아,김정만,이광묵 가톨릭대학 산업의학쎈타 산업의학연구소 1986 韓國의 産業醫學 Vol.25 No.3

For the purpose of obtaining of an effective biological monitoring data in terms of evaluation the health consequences of lead absorption, the female workers in the electronic industry who have been exposed to low-level lead occupationally were chosen as the subjects of our studies. We observed the 9 parameters in not only 305 lead-exposed female workers who have been exposed to lead occupationally also 254 normal female subjects who have not been exposed to lead occupationally. The parameters chosen for this study were as follows, blood lead (PbB), urine lead (PbU), δ-aminolevulinic acid in urine(ALAU), coproporphyrin in urine(CPU), zinc protoporphyrin in whole blood(ZPP), hemoglobin(Hb), hematocrit(Ht), δ-aminolevulinic acid dehydratase(ALAD) activity & ALAD activity ratio(A/R; absorbance at substrate pH 6.6/absorbance at substrate pH 5.8). The results obtained were as follows: 1. The average age of the lead-exposed female workers in the electronic industry and in the normal female subjects were 20.5±4.2 years and 21.7±3.5 years, respectively. 2. The average work duration of the lead-exposed female workers in the electronic industry was 26.4±19.4 months 3. The mean value of PbB of the lead-exposed female workers in the electronic industry and in the normal female subjects were 30.18±6.64㎍/100ml and 21.61±3.84㎍/100ml respectively. 4. In the lead-exposed female workers of the electronic industry and of the normal female subjects, the mean value of ZPP were 36.22±0.69㎍/100ml and 24.64±7.21㎍/100ml, respectively 5. In the lead-exposed female workers of the electronic industry and of the normal female subjects, the mean value of Hb were 13.98±0.57g/100ml and 14.09±0.66g/100ml, respectively. 6. The mean value of the lead-exposed female workers in the electronic industry were 55.07±17.61㎍/1 for PbU, 1.93±0.69mg/1 for ALAU, and 49.74±22.79㎍/1 for CPU, while in the normal female subjects, 44.27±15.76㎍/1 for PbU, 1.46±0.55mg/ 1 for ALAU, and 38.66±17.99㎍/1 for CPU. 7. Among various pairing of the parameters of lead exposure in the female lead-exposed workers and in the normal female subjects, the correlation between PbB and ZPP showed the highest coefficient value.

친환경농업 시범마을에 대한 환경영향평가

이남종,고병구,노기안,한민수,김민경,곽한강,박문희 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

1999년부터 2001년까지 3년간 친환경농업 시범마을에 적용되고 있는 INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) 실천에 따른 추진효과를 분석하기 위하여 시범마을로 지정된 옥천과 양평지역의 농가 포장에서 시비량, 양분유실 및 생태계 변화 등을 조사 분석하였다. 3요소 시비량은 토양검정시비로 인하여 농가관행시비와 비교하여 28.6~39.4% 절감할 수 있었으며, 쌀 수량도 시비량이 비교적 적은 토양검정 시비구에서 3~10% 증수하였다. 영농기간동안 토양 침투수중 NO₃-N 농도는 농가관행 시비구 2.9 ㎎/L에 비하여 토양검정 시비구는 1.5 ㎎/L로 감소하였으며, 시범마을 논으로 유입되는 관개수의 COD, NH₄-N, NO₃-N 농도는 각각 15.0, 0.67, 1.39 ㎎/L, 시범마을 논에서 배출된 용수는 각각 12.4, 0.29, 2.42 ㎎g/L로 감소되는 경향이었다. 처리별 수서곤충, 환형동물, 패류 및 감각류 등의 개체수는 농가관행구 100에 대하여 토양검정 시비구 293, 토양검정과 개량제 혼용구 276, 무비구 90으로 토양검정 시비구에서 많았으며 수서 무척추 동물은 관행농업을 수행하고 있는 지역을 100으로 볼 때 친환경농업마을은 242와 443으로 INM과 IPM을 실천하는 시범마을에서 생물종이 다양하고 그 개체수가 많은 것으로 나타났다. 친환경농업 시범마을 조성 후 작물 추천시비량 준수와 적기 예찰을 통하여 필요이상 사용되는 비료와 농약을 절감하여 생태계의 개선과 토양이 건전화된 것으로 평가되었다. In order to preserve the soundness of ago-ecosystem and products safety in agriculture, this study was evaluated environmental impact in relation to the INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) at paddy fields at Ok-Chun and Yang-Pyung region. By introduction of INM with the application of BB(bulk-blanded) fertilizer based on soil analysis and EM, the application rate of fertilizer was reduced to about 28.6-39.4% and the yield of brown rice was increased to about 3-10% compared to conventional practices. The concentrations of COD, NH4-N, and NO3-N in irrigation water flowed to the environmental-friendly agriculture practices were 15.0, 0.67, and 1.39 ㎎/L, respectively. The concentrations of COD, NH₄-N, and NO₃-N from paddy fields in drainage water were 12.4, 0.29, and 2.42 ㎎/L, respectively. The total number of the freshwater invertebrates was higher in field treated with fektilhtion by prescription with soil testing. Also, the population density of aquatic insects was higher than the other fields at both demonstration villages. In conclusion, it was possible to reduce the amount of fertilizer and agricultural chemicals application, and increase the yield of rice by application of the environmental-friendly agriculture practices.

肥滿 CLINIC 來院患者 453 CASES에 對한 臨床的 考察

이남구,안경순 又石大學校 1993 論文集 Vol.15 No.-

In 1991, Obesity rate of South Korea has reached to 18.7%. Because of economical development, the pattern of diet is exchanged from carbohydrate to rich protein and fat. The more problem is not only obesity of adult but also one of little child. Obesity is induced to diabetes mellitus, hypertension, artherosclerosis, hyperlipoidemia, heart and C.V.A disease, etc. In Woman, special important problem is the complex of beauty about woman's figure. In Oriental Medicine, the factor of obesity is mainly regarded as dampness. And there are many treatments and methods to body weight loss, but obesity patients dislike to use them because of their side effects and inconvenience, intolerance. Now ear acupuncture is applied on so many disease because of its easy handly, non-side effect and high efficiency in clinics. Here obesity acupuncture is used to ear and whole body acupuncture. Because they react eachother for their lack point. Therefore, in order to investigate the effect of obesity acupuncture and develop non-drug, non-starvation etc, we analyzed 453 the cases of body weight loss patients treated with ear and whole body acupuncture in Oriental Medicine Hospital in Jeon-Ju Woo-Sug University from April.1.1992. to March. 17.1993. The results were summarized as follows. 1. Distribution of sex ; Male (4.4%), Female (95.6%) 2. Distribution of age in descending order ; 30s, 20s, 40s, 10s, 50s, below 10s, above 60s. The 20s-30s are group made up 60.7% of the group. 3. Distribution of occupation in descending order ; housewife, student, service, salaried, merchant, teacher, farmer, inoccupation. 4. Distribution of human coporal constitution in descending order ; Tae-Eum-In, So-Eum-In, So-Yang-In. 5.Distribution of body height and weight, 155-164cm ; 71.1%, 60-70kg, 74.6% are majority. 6. Distribution of weight variation, 2-6kg(71.0%) is majority, also 13-14kg(0.4%). 7. Distribution of duration in descending order ; 1-3 years, 3-6 years, 1-12 months, above 10 years but in success, 1-12 months, 1-3 years, 3-6 years, above 10 years. Therefore, we know that the shorter duration of obesity is, the more loss of body weight. 8. Past experiences to body weight loss; Yes(69.5%), No(30.5%). The success rate accordant with the past temporay experiences shows that the cases without experience is higher than the ones with experience. 9. In distribution of times(treatments), 10 times is top. the rate of body weight loss is the highest in 14 times. Therefore, I think that one would need at least 10 times, in order to lose body weight. 10. Distribution of body weight variation in treatments times is at 2 times(3-4kg loss), and surprisingly is 14kg loss at above 15 times. 11. Distribution of symptoms improvement, in descending order ; heavy sense in body, dec. of appetite, inc. of exercise, lumbago, edema, knee pain, inc. of urine, inc. of fullness sense, thirsty, disease of gynecology, white tung, chest burning, heart burning, dec. of tobacco, drink taste, motion sickness, allergy, water eczema, arthma, belching. 12. Distribution of snack ; Yes(87.4%), No(12.6%) 13. Distribution of exercise ; Yes(21.4%). No(78.6%) 14. Distribution of sleeping times, above 7 hours(79.0%) 15. Distribution of reason to body loss, the complex of beauty(68.7%) is top. 16. Distribution of side effect in obesity acupuncture, constipation(17.4%) is top. 17. Distribution of method in body weight loss ; dietary treatment(31.1%), sauna(26.7%), exercise(19.7%), the center of body weight loss(15.0%) herb-med and starvation treatments(5.1%), hand-finger acupuncture(hand-foot acupuncture) is 1.6%, diet pill(0.3%), etc(0.6%)

영병에 關한 文獻的 考察

南泰宣,李光揆,李南九 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

영병은 頸部의 結候兩側에 腫瘍이 발생되는 질병을 總稱하는 것으로 包括的인 면으로 볼 때 西洋醫學적으로 볼 때 甲狀腺腫大와 같은 것으로 본다. 이러한 병에 對하여 이미 오래전부터 地理적 環境과의 相關關係를 言及하였을 뿐만 아니라 좀더 나아가서는 情志失調도 이병을 일으키는 主要原因이 된다고 보았다. 現代醫學에 있어서는 甲狀腺腫大는 갑상선기능항진증에서 볼수 있는 것으로 보아 영병과 갑상선기능항진증과의 密接한 關係가 있음을 暗示해 주고 있다. 이와같이 古典적인 病名과 現代적인 症狀과의 比較分析을 위해 文獻考察方法도 韓醫學發展에 寄與할것으로 思慮된다. Goiter means all diseases causing tumor on the both sides of Adam's apple, and in the western medicine it is similar to tumefaction in thyroid gland. In the past the correlation between geographical features of deficiency of the kidney Yin as an important cause. In the present medicine, they suggest there should be close relation between goiter and hyperthyroidism. Therefore the comparative literature study on the ancient names of disease and present symptoms will contribute to the development of the Korean oriental medicine. The results of this study are summarized as follows: 1. In the past, the stagnation of qi and phlegm due to soil and water-borne diseases, and damage on being the response of the mind to the environmental stimuli was considered as the greatest causes of goiter, whereas in the present they give attention to goiter's symptoms caused by deficiency of Yin and think of deficiency of the kidney yin as important cause. 2. The ancient medicine books described the symptoms centering on the outer signs having tumefaction of diffuse on the neck, however modern oriental medicine writes symtoms of the whole body caused hyperactivity of yang due to yin deficiency inqi goiter addition to the outer symtoms and they are very similar to hyperthyroidism. 3. For the treatment, according to the treatment for the differentiation of symptoms and signs, they mainly use smoothing the liver and regulating the circulation of qi for the stagnation of qi; promoting blood circulation to remove blood stasis for the blood stasis; resolving sputum and softening hard masses for the lumps of phlegm; removing heat phlegm for the stagnation of phlegm fire, however in recent books we can find many ways for the nourishing Yin and prescription.

地方自治와 새마을 運動에 관한 硏究

李南久 경북대학교 1986 새마을 硏究論叢 Vol.6 No.-

다변량해석기법을 이용한 만경강水系의 水質評價

오연찬,이남도,김종구 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

This study was conducted to evaluate water quality in the Mankyung River using multivariate analysis. The analysis data which was surveyed from January 1996 to December 2002 in Mankyung river was aquired by the ministry of environment. Twelve water quality parameters were determined on each survey. The results were summarized as follow ; Water quality in the Mankyung River could be explained up to 74.90% by four factors which were included in loading of organic matter and nutrients by the tributaries(43.28%), seasonal variation(10.40%), loading of pathogenic bacteria by domestic sewage of Gapcheon (12.41%) and internal metabolism in river(8.81%). The result of cluster analysis by station was classified into three group that has different water quality characteristics. Especially, Iksan river was appeared to considerable water quality characteristics against other station. In monthly cluster analysis, three group was classified by seasonal characteristics. Also, in yearly cluster analysis, three group was classified. It is necessary to control the pollutant loadings by domestic sewage and livestock waste for water quality management of Mankyung river.

針灸學 發達의 文獻的 考察

宋範龍,李南九 又石大學校 1995 論文集 Vol.17 No.-