소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

다단계 검출알고리즘을 이용한 웨이블렛기반 이미지 워터마킹

이구영,정용덕,이종원,김정화 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.2

In this paper, we propose a watermarking algorithm of multiresolution watermarking based on wavelet transform for digital images. Watermark insertion is add to pseudo random sequences to the wavelet coefficients at the high and middle frequency band of the discrete wavelet transform. Instead of previous method, proposed multiresolution watermark decoder that allows efficient identification of the embedded a bank of watermarking key without prior notice of it. Proposed detection method aims at ensuring the maximum exactitude in the detection of the owner identification key and, minimizing the number of false positive detection.

市·郡 統合型 地域農業의 組織化에 관한 硏究

이기웅,박종구 순천대학교 지역개발연구소 1997 地域開發硏究 Vol.8 No.-

Every circumstance surrounding Korean agriculture is getting worse and unfavorable. First of all, in view of domestic situation, Korean agriculture faces widespread small scale farming, greater proportion of an old labor force, rice monoculture, stagnant and neglible investments, and the poor infrastructure in support of agricultural production. Thus, the agricultural and rural areas remain underdeveloped with low productivity. In addition to the challenging internal problems of agriculture, the agricultural industry is not internationally competitive and not well prepared for the market liberalization. It is clear that, under open international competition, many low productivity farms would be forced out of business, and the resulting social cost and unrest would be significant. In this point of view Korean government has enforced urban-rural integration Under these considerations, the main objectives of this paper are 1) to analyze the regional agricultural structure of urban-rural integration(Sunchon City) and 2) to find out the organization direction of regional agriculture to urban-rural integration. In conclusion, in order to organize regional agriculture to urban-rural integration, we should make all-out efforts such as Production-Pursuiting Type, Marketing-Oriented Type, and Regional Self-Supporting Type.

農業法人 經營體의 運營實態와 育成方向

이기웅,박종구 순천대학교 지역개발연구소 1998 地域開發硏究 Vol.9 No.-

In order to deal with the rapidly changing environment such as trade liberalization and the problem of local autonomy, the Korean government has implemented a comprehensive master policy entitled, "Agricultural Development Plans". It especially focuses on improving the agricultural structure and enhancing the farmers' living standard. In 1994, the government issued a new plan "Agriculture and Fisheries Development Plan(AFDP)". Increasing concern from the rural dwellers about the future of their life has made the government establish new policy, focusing on more welfare-oriented program. These changes and other pressures indicate that agriculture should move in the direction of less cost, improved efficiency, transparency and consistency, and market-orientation. Most have recommended general and widespread reform of agricultural policies based on market-oriented direction with competitiveness in world market. The purposes of this study are to identify the operating situation of farming corporation and to suggest future direction to improve the agricultural structure so that it meets the requirements of a competitive agriculture under the WTO system.

성인 급성 신부전의 원인 및 예후인자 분석

구영선,장윤경,양종오,강민규,황평주,김종학,나기량,이강욱,신영태 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Acute renal failure is a frequent complication in hospitalized patients and is strongly related to the mortality. The Clinical outcome and prognostic factors of acute renal failure(ARF) have been analyzed by many authors. The present study describes the etiologic and clinical aspects, as well as other factors related to mortality. All the patients suffering from acute renal failure admitted during the period of January 1993 - August 1998 were included in the study. The average age of the patients was 52±17 years and mortality rate was 24%. The causes of acute renal failure were hemorrhagic fever with renal syndrome(HFRS), sepsis, renal hypoperfusion, urinary tract obstruction, acute tubular necrosis, etc. The etiology of ARF was a significant prognostic factor on mortality in ARF. Other significant prognostic factors were oliguria, organ failure, use of vasoconstrictors, hypotension, serum bicarbonate, premorbid conditions, sepsis, neurologic complications, gastrointestinal bleeding. On the other hands, operation, sex, anemia, thrombocytopenia, hypoalbuminemia, BUN, serum creatinine, and hyperkalemia were not significant factors for the mortality. We conclude that major prognostic factors of acute renal failure arc premorbid conditions, sepsis and multiorgan failure, and they are responsible for persistent high mortality of acute renal failure despite of advances of medical care.

BGA 솔더 볼 로딩장치의 최적설계

이준환,이종원,구홍모 중앙대학교 생산공학연구소 1999 생산공학연구소 논문집 Vol.8 No.2

최근의 정보화 장비와 멀티미디어의 소형화 추세는 생산 설비중 반도체 패키지 기술레 힘입은 바 크다. BGA(Ball Grid Array types of package)와 CPS(Chip Scale Packages)는 QFP(Quad Flat Packages)를 대체하는 기술로 인정되고 있다. 볼을 탑재하는 장치는 기존에 진공 - 흡입장치가 많이 사용되어 왔다. 시소 타입 로더에서는 플래이트의 각도와 카트리지의 속도가 중요한 요소로 간주된다. 본 연구에서는 이에 대한 최적 속도와 각도를 제안하였다. With the trends of the recent micro-miniaturization of mutimedia and information equipment, electronic components packaging technology is playing a key role in production technology. BGA(Ball Grid Array thpes of package) and CSP(Chip Scale Packages) are replacing QFT(Quad Flat Packages). Vacuum-suction type loader is a popular loading device. For the seesaw type loader, the velocity fo cartridge and the angle of plate are very important elements. In this study, the optimal values of the velocity and the angle are proposed for the seesaw type loader.

이식 탈회골의 골화에 대한 실험적 연구

구순상,이종원,이택호,신태선 大韓成形外科學會 1984 Archives of Plastic Surgery Vol.11 No.2

The most effective osseous graft material is the fresh autogenous bone in clinical practice and experimental studies. However, there are some problems in use of fresh autogenous bone graft, such as donor-site morbidity and the limitation in amount of available autogenous bone. Since the introduction of decalcified bone as grafting material by Senn(1889), decalcified bone has been studied as substitute for autogenous bone. The implantation of decalcified bone segment or powder in soft tissue has been established as a laboratory model for studying bone induction. Bone powders (particle size: 74∼420㎛) of rat and ox were decalcified with 0.5M HCI and were implanted i rat's abdomen subcutaneously. Thereafter, periodic observation was made histologically up to 30 days. Comparative studies were carried out to determine differences in histological sequence of induced osteogenesis and quantity of new bone. The results were as follows; 1. Implantation of decalcified bone powders of rat and ox to subcutaneous site in rats resulted in new bone formation by an enchondral sequence and there were no differences in histological change periodically. 2. There were no differences in quantity of new bone.

가공속도와 슬러리의 가공온도에 따른 웨이퍼 폴리싱의 표면특성에 관한 연구

이정택,원종구,신태희,이은상 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

Silicon wafers which are the starting materials for the industry of semiconductor must be very flat in order to print circuits on them by lithographic processes. Therefore, the wafer polishing is an essential process used in the fabrication of silicon wafers to achieve a globally planar and mirror-like wafer surface. The surface roughness in the wafer depends on the surface properties of the carrier head unit along with other machining conditions such as polishing pad, Processing velocity, machining temperature, down-force distribution and so on. Also the applied down-force is an important parameter of polishing process. This study shows the surface characteristics between the polishing pad and the wafer carrier unit by wafer polishing system head. The experiments were performed to observe the down-force and temperature when the wafer carrier head unit was pressed down onto the polishing pad. To measure the down-force distribution in processing velocity, the experimental set up used the loadcell to get the signal of the applied pressure against the polishing pad in actual processing with detecting temperature in slurry. Thus the optimum condition selection of ultra precision wafer polishing is using loadcell and infrared temperature sensor. The optimum condition is selected to use a result data that measure a pressure in processing velocity. By using optimum condition, it helps to achieve an ultra precision mirror like surface.

Pulse Oximeter를 이용한 치수생활력측정

이제호,구본경,이종갑 大韓小兒齒科學會 2000 大韓小兒齒科學會誌 Vol.27 No.1

치수 생활력 검사시 전통적인 방법으로 전기치수검사나 온도변화검사 등이 있다. EPT와 ice test 즉, 전기치수검사와 온도변화검사는 치아의 신경학적 반응에 의해 치아의 실활여부를 판단하는 방법으로 환자가 아동일 때는 정확한 반응을 얻기가 어렵고, 환자의 주관적 반응을 판단해야 하므로 객관적이지 못하고, 소아환자에게 좋지 못한 자극을 주어 행동 조절의 문제를 일으키며, 거짓 양성반응과 거짓 음성반응이 나올 수 있다는 등의 한계가 있다. 최근에는 전통적인 방법의 한계를 극복하려는 시도에서 혈관의 보전성을 평가하는 방법인 laser doppler flowmetry와 pulse oximeter를 이용하는 방법이 소개되고 있다. Pulse oximeter의 원리는 두 가지 종류의 파장의 빛을 귀, 손가락 등 생체의 말단에 투과시켜 발산된 빛과 감지된 빛간의 두 파장의 흡수비로 산소포화 정도를 알아내는 것으로서 이에 착안하여 또 하나의 생체 말단인 치아에 이를 적용하여 치아의 실활 여부를 판단하는 것이다. 이 보고에서는 치수 생활력 검사시 pulse oximeter의 사용 가능성에 대해 검증하고 이의 임상적용에 대해 기초적 자료를 제공하고자 함을 목적으로 했는데 생활치에서는 평균 96.3%의 산소 포화도를 실활치에서는 평균 0.0%의 산소 포화도를 얻어냄으로서 pulse oximeter가 치아의 실활여부 판단에 있어 유용한 진단도구로서의 가치가 있다는 것이 밝혀졌다. Traditionally, EPT and thermal tests were used as diagnostic methods for pulp vitality test. The thermal and electrical stimulation tests are the methods to determine the vitality of a tooth based on its neuronal response. These have certain limitations, one of them is the difficulty of approaching the correct result in case of treatment of children. The reason is management problem caused by the unpleasant stimulation. Also, the response from patients are not objective, and false positive or false negative could be happened. Recently, laser doppler flowmetry and pulse oximeter which evaluate vascular integrity are introduced-in an effort of overcoming to limitation of traditional methods. The principle of pulse oximeter is to find out level of oxygen saturation by ratio of the two pulses between emitted light and detected light penetrating them to the termination of body, such as ears or fingers. From this point of view, it can be applied to a tooth to determine its vitality. The objective of this study lies mainly on varifying pulse oximeter as a method of determining tooth vitality and providing basic data of its clinical implementation. The result of the research showed that level of oxygen saturation in vital teeth was average of 96.3% and 0.0% in pulpless teeth. As a comprehensive result, pulse oximeter could be an useful diagnostic equipment in determining of tooth vitality.

웨이퍼 폴리싱의 가공 조건에 따른 표면 특성에 관한 연구

이정택,원종구,이은상 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-

The wafer polishing is an essential process used in the fabrication of silicon wafers to achieve a globally planar and mirror-like wafer surface. The surface roughness in the wafer depends on the surface properties of the carrier head unit along with other machining conditions such as polishing pad, working speed, temperature, down-force and ETC. Also the applied down-force is an important parameter of polishing process. This study shows the surface characteristics between the polishing pad and the wafer carrier unit by wafer polishing system head. The experiments were performed to observe the down-force and temperature when the wafer carrier head unit was pressed down onto the polishing pad. To measure the down-force distribution, the experimental set up used the loadcell to get the signal of the applied pressure against the polishing pad in actual processing with detecting temperature using IR temperature sensor.